Restriction map for
pFN21AB8453
(Circular) (Six-base) MAPSORT of: pFN21AB8453.seq Check: 6001 from: 1 to: 5668
pFN21AB8453 5668 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2499 3478 278
Size: 53 83 186 936 963 979 2468
Fragments arranged by size:
2468 979 963 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3011 1937
Size: 1074 4594
Acc65I G'GTAC_C
Cuts at: 2079 2989 2079
Size: 910 4758
AclI AA'CG_TT
Cuts at: 1563 3795 4168 1563
Size: 2232 373 3063
Fragments arranged by size:
3063 2232 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3731 5115 5383 1632
Size: 2099 1384 268 1917
Fragments arranged by size:
2099 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5471
AflIII A'CryG_T
Cuts at: 1203 2232 4567 5544 1203
Size: 1029 2335 977 1327
Fragments arranged by size:
2335 1327 1029 977
AgeI A'CCGG_T
Cuts at: 4499 4499
Size: 5668
AhdI GACnn_n'nnGTC
Cuts at: 4397 4397
Size: 5668
AleI CACnn'nnGTG
Cuts at: 1166 2944 1166
Size: 1778 3890
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5668
AlwNI CAG_nnn'CTG
Cuts at: 2470 2791 3411 4983 2470
Size: 321 620 1572 3155
Fragments arranged by size:
3155 1572 620 321
ApaI G_GGCC'C
Cuts at: 2148 2876 2148
Size: 728 4940
ApaLI G'TGCA_C
Cuts at: 3725 4881 3725
Size: 1156 4512
ApoI r'AATT_y
Cuts at: 1493 2977 3203 3355 5464 1493
Size: 1484 226 152 2109 1697
Fragments arranged by size:
2109 1697 1484 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4222 160
Size: 872 3190 1606
Fragments arranged by size:
3190 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5668
AvaI C'yCGr_G
Cuts at: 1870 1943 2120 2140 2193 2964 2993 1870
Size: 73 177 20 53 771 29 4545
Fragments arranged by size:
4545 771 177 73 53 29 20
BamHI G'GATC_C
Cuts at: 2998 3365 2998
Size: 367 5301
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2079 2960 2989 4444 618
Size: 325 217 627 292 881 29 1455 1842
Fragments arranged by size:
1842 1455 881 627 325 292 217 29
BanII G_rGCy'C
Cuts at: 1391 2041 2067 2148 2876 2987 1391
Size: 650 26 81 728 111 4072
Fragments arranged by size:
4072 728 650 111 81 26
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2164 2192 2260 928
Size: 971 265 28 68 4336
Fragments arranged by size:
4336 971 265 68 28
BbvCI CC'TCA_GC
Cuts at: 2792 2825 2792
Size: 33 5635
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3859 3893 1903
Size: 34 50 34 1838 34 3678
Fragments arranged by size:
3678 1838 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3560 4770 3560
Size: 1210 4458
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5668
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4279 136
Size: 107 122 71 631 605 2607 1525
Fragments arranged by size:
2607 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5663 5663
Size: 5668
BlpI GC'TnA_GC
Cuts at: 2126 3085 2126
Size: 959 4709
Bme1580I G_kGCm'C
Cuts at: 1790 2148 2876 2965 3729 4885 1790
Size: 358 728 89 764 1156 2573
Fragments arranged by size:
2573 1156 764 728 358 89
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5668
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2315 2601 3399
Size: 416 53 83 399 24 898 286 798
Cuts at: 3399 4357 442
Size: 958 1753
Fragments arranged by size:
1753 958 898 798 416 399 286 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5668
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2060 2914 2060
Size: 854 4814
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2055 2237 2279 2795 2884 4328 1239
Size: 816 182 42 516 89 1444 2579
Fragments arranged by size:
2579 1444 816 516 182 89 42
Bpu10I CC'TnA_GC
Cuts at: 2151 2408 2792 2825 2151
Size: 257 384 33 4994
Fragments arranged by size:
4994 384 257 33
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3793 4658 4956 3793
Size: 865 298 4505
Fragments arranged by size:
4505 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2771 4331 882
Size: 617 1272 1560 2219
Fragments arranged by size:
2219 1560 1272 617
BsaAI yAC'GTr
Cuts at: 493 1204 2235 3459 493
Size: 711 1031 1224 2702
Fragments arranged by size:
2702 1224 1031 711
BsaBI GATnn'nnATC
Cuts at: 5662 5662
Size: 5668
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2496 3475 3857 4492
Size: 53 83 186 936 963 979 382 635
Cuts at: 4492 275
Size: 1451
Fragments arranged by size:
1451 979 963 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4101 4499 4773 4920 5331 4101
Size: 398 274 147 411 4438
Fragments arranged by size:
4438 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5443
Fragments arranged by size:
5443 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2033 2903 1186
Size: 847 870 3951
Fragments arranged by size:
3951 870 847
BseYI C'CCAG_C
Cuts at: 2353 2513 2731 2750 2952 2956 4871 5367 2353
Size: 160 218 19 202 4 1915 496 2654
Fragments arranged by size:
2654 1915 496 218 202 160 19 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2686 2784 1163
Size: 573 950 98 4047
Fragments arranged by size:
4047 950 573 98
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2740 3118 3879 4907 1540
Size: 275 186 739 378 761 1028 2301
Fragments arranged by size:
2301 1028 761 739 378 275 186
BsiHKAI G_wGCw'C
Cuts at: 3729 3814 4885 3729
Size: 85 1071 4512
Fragments arranged by size:
4512 1071 85
BsmI GAATG_Cn'
Cuts at: 3179 3272 3179
Size: 93 5575
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2041 2067 2148 2876 2965 2987 3729
Size: 399 251 26 81 728 89 22 742
Cuts at: 3729 3814 4885 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 728 399 251 89 85
81 26 22
BspEI T'CCGG_A
Cuts at: 5331 5331
Size: 5668
BspHI T'CATG_A
Cuts at: 3557 5287 3557
Size: 1730 3938
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3023 844
Size: 2179 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3555 1394
Size: 429 1732 3507
Fragments arranged by size:
3507 1732 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2175 4163 4337 65
Size: 1183 927 1988 174 1396
Fragments arranged by size:
1988 1396 1183 927 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2013 2523 4312 4499 1700
Size: 110 203 510 1789 187 2869
Fragments arranged by size:
2869 1789 510 203 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5668
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5668
BssSI C'ACGA_G
Cuts at: 2563 3728 4740 2563
Size: 1165 1012 3491
Fragments arranged by size:
3491 1165 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5668
BstBI TT'CG_AA
Cuts at: 4472 4472
Size: 5668
BstEII G'GTnAC_C
Cuts at: 1184 2323 2818 1184
Size: 1139 495 4034
Fragments arranged by size:
4034 1139 495
BstYI r'GATC_y
Cuts at: 1972 2998 3365 3750 3767 5219 5305 5317 5663
Size: 1026 367 385 17 1452 86 12 346
Cuts at: 5663 1972
Size: 1977
Fragments arranged by size:
1977 1452 1026 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2021 2337 2642 2706 513
Size: 552 956 316 305 64 3475
Fragments arranged by size:
3475 956 552 316 305 64
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4929
Fragments arranged by size:
4929 683 56
BtsI GCAGTG_nn'
Cuts at: 2015 2187 3184 3978 3998 2015
Size: 172 997 794 20 3685
Fragments arranged by size:
3685 997 794 172 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5668
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5633
DraI TTT'AAA
Cuts at: 2972 2972
Size: 5668
DraIII CAC_nnn'GTG
Cuts at: 2320 2320
Size: 5668
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4675 809
Size: 507 3359 1802
Fragments arranged by size:
3359 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2397 2737 3115
Size: 54 1425 50 275 18 567 340 378
Cuts at: 3115 4004 5633 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 567 378 340 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2737 3115 1537
Size: 275 925 378 4090
Fragments arranged by size:
4090 925 378 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3598 1353
Size: 2245 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4255 4629 4775 387
Size: 1064 138 2666 374 146 1280
Fragments arranged by size:
2666 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2055 2237 2279 2795 2884 3731 4328
Size: 393 423 182 42 516 89 847 597
Cuts at: 4328 5115 5383 1239
Size: 787 268 1524
Fragments arranged by size:
1524 847 787 597 516 423 393 268
182 89 42
EcoO109I rG'GnC_Cy
Cuts at: 2026 2115 2144 2145 2310 2872 2873 2893 2026
Size: 89 29 1 165 562 1 20 4801
Fragments arranged by size:
4801 562 165 89 29 20 1 1
EcoRI G'AATT_C
Cuts at: 2977 3355 2977
Size: 378 5290
FspI TGC'GCA
Cuts at: 4174 4174
Size: 5668
HaeII r_GCGC'y
Cuts at: 1678 2459 4815 1678
Size: 781 2356 2531
Fragments arranged by size:
2531 2356 781
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4309 4341 4383 4415 1373
Size: 32 135 32 2737 32 42 32 2626
Fragments arranged by size:
2737 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3012 3258 669
Size: 988 281 1074 246 3079
Fragments arranged by size:
3079 1074 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5668
HpaI GTT'AAC
Cuts at: 3258 3258
Size: 5668
KpnI G_GTAC'C
Cuts at: 2083 2993 2083
Size: 910 4758
MfeI C'AATT_G
Cuts at: 3267 3267
Size: 5668
MluI A'CGCG_T
Cuts at: 5544 5544
Size: 5668
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4782 4966 1305
Size: 3477 184 2007
Fragments arranged by size:
3477 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2399 10
Size: 54 1425 343 567 3279
Fragments arranged by size:
3279 1425 567 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2180 2944 3626 3985 4144 518
Size: 648 1014 764 682 359 159 2042
Fragments arranged by size:
2042 1014 764 682 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2023 2104 2339 2485 2788 2956 3084 3761
Size: 417 81 235 146 303 168 128 677
Cuts at: 3761 4909 5154 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 417 303 245 235 168
146 128 81
NaeI GCC'GGC
Cuts at: 2015 2015
Size: 5668
NcoI C'CATG_G
Cuts at: 513 1065 2706 513
Size: 552 1641 3475
Fragments arranged by size:
3475 1641 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5668
NgoMIV G'CCGG_C
Cuts at: 2013 2013
Size: 5668
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5668
NotI GC'GGCC_GC
Cuts at: 2737 3115 2737
Size: 378 5290
NspI r_CATG'y
Cuts at: 1132 1229 3026 4571 1132
Size: 97 1797 1545 2229
Fragments arranged by size:
2229 1797 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5668
PciI A'CATG_T
Cuts at: 4567 4567
Size: 5668
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2320 1108
Size: 1212 4456
PfoI T'CCnGG_A
Cuts at: 2243 2295 2243
Size: 52 5616
PmeI GTTT'AAAC
Cuts at: 2972 2972
Size: 5668
PmlI CAC'GTG
Cuts at: 2235 2235
Size: 5668
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3737 3769 5250 5282 3737
Size: 32 1481 32 4123
Fragments arranged by size:
4123 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2115 2310 2893 2115
Size: 195 583 4890
Fragments arranged by size:
4890 583 195
PshAI GACnn'nnGTC
Cuts at: 1613 2264 2308 1613
Size: 651 44 4973
Fragments arranged by size:
4973 651 44
PsiI TTA'TAA
Cuts at: 3238 3238
Size: 5668
PspOMI G'GGCC_C
Cuts at: 2144 2872 2144
Size: 728 4940
PstI C_TGCA'G
Cuts at: 830 2466 2748 2787 3020 830
Size: 1636 282 39 233 3478
Fragments arranged by size:
3478 1636 282 233 39
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5668
PvuII CAG'CTG
Cuts at: 2788 2956 2788
Size: 168 5500
SacII CC_GC'GG
Cuts at: 2024 2340 2024
Size: 316 5352
SalI G'TCGA_C
Cuts at: 1936 3010 1936
Size: 1074 4594
SanDI GG'GwC_CC
Cuts at: 2115 2310 2115
Size: 195 5473
SbfI CC_TGCA'GG
Cuts at: 3020 3020
Size: 5668
ScaI AGT'ACT
Cuts at: 3916 5628 3916
Size: 1712 3956
SfcI C'TryA_G
Cuts at: 826 1045 2156 2462 2744 2783 2867 3016 4151
Size: 219 1111 306 282 39 84 149 1135
Cuts at: 4151 4832 5023 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1111 681 306 282 219 191
149 84 39
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5668
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5668
SmaI CCC'GGG
Cuts at: 1872 2142 2195 2995 1872
Size: 270 53 800 4545
Fragments arranged by size:
4545 800 270 53
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3772 4673 4935 820
Size: 197 926 1829 901 262 1553
Fragments arranged by size:
1829 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3459 493
Size: 2966 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5668
SphI G_CATG'C
Cuts at: 1132 3026 1132
Size: 1894 3774
SspI AAT'ATT
Cuts at: 5 52 3592 5
Size: 47 3540 2081
Fragments arranged by size:
3540 2081 47
StuI AGG'CCT
Cuts at: 1517 2292 1517
Size: 775 4893
StyI C'CwwG_G
Cuts at: 513 1065 2450 2706 3107 513
Size: 552 1385 256 401 3074
Fragments arranged by size:
3074 1385 552 401 256
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2367 2948 3695 3712 3865 4050 4495
Size: 1255 787 581 747 17 153 185 445
Cuts at: 4495 325
Size: 1498
Fragments arranged by size:
1498 1255 787 747 581 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3914 5626 96
Size: 274 80 33 51 1443 1937 1712 138
Fragments arranged by size:
1937 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5628 5660 5628
Size: 32 5636
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5668
XbaI T'CTAG_A
Cuts at: 3004 3004
Size: 5668
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2940 2940
Size: 5668
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5668
XmaI C'CCGG_G
Cuts at: 1870 2140 2193 2993 1870
Size: 270 53 800 4545
Fragments arranged by size:
4545 800 270 53
XmnI GAAnn'nnTTC
Cuts at: 3797 3797
Size: 5668
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2497 3476 276
Size: 53 83 186 936 963 979 2468
Fragments arranged by size:
2468 979 963 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PspXI PsrI RsrII SacI SapI SexAI SfiI SfoI
SrfI SwaI