Restriction map for
pFN21AB8448
(Circular) (Six-base) MAPSORT of: pFN21AB8448.seq Check: 3375 from: 1 to: 6040
pFN21AB8448 6040 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2159 2159
Size: 6040
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3850 278
Size: 53 83 186 936 2314 2468
Fragments arranged by size:
2468 2314 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3383 1937
Size: 1446 4594
Acc65I G'GTAC_C
Cuts at: 3361 3361
Size: 6040
AclI AA'CG_TT
Cuts at: 1563 4167 4540 1563
Size: 2604 373 3063
Fragments arranged by size:
3063 2604 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4103 5487 5755 1632
Size: 2471 1384 268 1917
Fragments arranged by size:
2471 1917 1384 268
AfeI AGC'GCT
Cuts at: 2126 2126
Size: 6040
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5843
AflIII A'CryG_T
Cuts at: 1203 2091 4939 5916 1203
Size: 888 2848 977 1327
Fragments arranged by size:
2848 1327 977 888
AgeI A'CCGG_T
Cuts at: 4871 4871
Size: 6040
AhdI GACnn_n'nnGTC
Cuts at: 4769 4769
Size: 6040
AleI CACnn'nnGTG
Cuts at: 1166 2153 1166
Size: 987 5053
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6040
AlwNI CAG_nnn'CTG
Cuts at: 3783 5355 3783
Size: 1572 4468
ApaI G_GGCC'C
Cuts at: 2300 2668 2300
Size: 368 5672
ApaLI G'TGCA_C
Cuts at: 4097 5253 4097
Size: 1156 4884
ApoI r'AATT_y
Cuts at: 1493 3349 3575 3727 5836 1493
Size: 1856 226 152 2109 1697
Fragments arranged by size:
2109 1856 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4594 160
Size: 872 3562 1606
Fragments arranged by size:
3562 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6040
AvaI C'yCGr_G
Cuts at: 1870 1943 3054 3365 1870
Size: 73 1111 311 4545
Fragments arranged by size:
4545 1111 311 73
BamHI G'GATC_C
Cuts at: 3370 3737 3370
Size: 367 5673
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2281 2372 2510 3361 4816
Size: 325 217 627 494 91 138 851 1455
Cuts at: 4816 618
Size: 1842
Fragments arranged by size:
1842 1455 851 627 494 325 217 138
91
BanII G_rGCy'C
Cuts at: 1391 2300 2668 3359 1391
Size: 909 368 691 4072
Fragments arranged by size:
4072 909 691 368
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2962 928
Size: 971 1063 4006
Fragments arranged by size:
4006 1063 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2047 2081 4231 4265 1903
Size: 34 50 34 26 34 2150 34 3678
Fragments arranged by size:
3678 2150 50 34 34 34 34 26
BciVI GTATCCnnnnn_n'
Cuts at: 3932 5142 3932
Size: 1210 4830
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6040
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2025 2330 2528
Size: 107 122 71 631 605 353 305 198
Cuts at: 2528 3194 4651 136
Size: 666 1457 1525
Fragments arranged by size:
1525 1457 666 631 605 353 305 198
122 107 71
BglII A'GATC_T
Cuts at: 6035 6035
Size: 6040
BlpI GC'TnA_GC
Cuts at: 3457 3457
Size: 6040
Bme1580I G_kGCm'C
Cuts at: 1790 2043 2300 2444 2668 4101 5257 1790
Size: 253 257 144 224 1433 1156 2573
Fragments arranged by size:
2573 1433 1156 257 253 224 144
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6040
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3102 3771 4729
Size: 416 53 83 399 24 1685 669 958
Cuts at: 4729 442
Size: 1753
Fragments arranged by size:
1753 1685 958 669 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6040
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2285 2861 2894 3233 3275 3299 4700 1239
Size: 1046 576 33 339 42 24 1401 2579
Fragments arranged by size:
2579 1401 1046 576 339 42 33 24
Bpu10I CC'TnA_GC
Cuts at: 2108 2108
Size: 6040
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4165 5030 5328 4165
Size: 865 298 4877
Fragments arranged by size:
4877 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4703 882
Size: 617 3204 2219
Fragments arranged by size:
3204 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2038 2356 2659 3831 493
Size: 711 834 318 303 1172 2702
Fragments arranged by size:
2702 1172 834 711 318 303
BsaBI GATnn'nnATC
Cuts at: 6034 6034
Size: 6040
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3847 4229 4864 275
Size: 53 83 186 936 2314 382 635 1451
Fragments arranged by size:
2314 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2421 3246 4473 4871 5145 5292 5703 2421
Size: 825 1227 398 274 147 411 2758
Fragments arranged by size:
2758 1227 825 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2687 2717 2828 2858 3299
Size: 30 165 30 1284 30 111 30 441
Cuts at: 3299 3329 1178
Size: 30 3889
Fragments arranged by size:
3889 1284 441 165 111 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2219 2450 2849 2990 3173 3182 3185 3245
Size: 1033 231 399 141 183 9 3 60
Cuts at: 3245 1186
Size: 3981
Fragments arranged by size:
3981 1033 399 231 183 141 60 9
3
BseYI C'CCAG_C
Cuts at: 2667 5243 5739 2667
Size: 2576 496 2968
Fragments arranged by size:
2968 2576 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5467
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3189 3490 4251 5279 1540
Size: 275 186 1188 301 761 1028 2301
Fragments arranged by size:
2301 1188 1028 761 301 275 186
BsiHKAI G_wGCw'C
Cuts at: 4101 4186 5257 4101
Size: 85 1071 4884
Fragments arranged by size:
4884 1071 85
BsmI GAATG_Cn'
Cuts at: 3551 3644 3551
Size: 93 5947
BsmBI CGTCTCn'nnnn_
Cuts at: 2387 2387
Size: 6040
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2043 2300 2444 2668 3359 4101 4186
Size: 399 253 257 144 224 691 742 85
Cuts at: 4186 5257 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 691 399 257 253 224
144 85
BspEI T'CCGG_A
Cuts at: 3246 5703 3246
Size: 2457 3583
BspHI T'CATG_A
Cuts at: 2181 2502 3929 5659 2181
Size: 321 1427 1730 2562
Fragments arranged by size:
2562 1730 1427 321
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2159 2637 2715 3395 844
Size: 1315 478 78 680 3489
Fragments arranged by size:
3489 1315 680 478 78
BsrBI CCG'CTC
Cuts at: 1394 1823 3927 1394
Size: 429 2104 3507
Fragments arranged by size:
3507 2104 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4535 4709 65
Size: 1183 3287 174 1396
Fragments arranged by size:
3287 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3032 4684 4871 1700
Size: 110 1222 1652 187 2869
Fragments arranged by size:
2869 1652 1222 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6040
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6040
BssSI C'ACGA_G
Cuts at: 2792 4100 5112 2792
Size: 1308 1012 3720
Fragments arranged by size:
3720 1308 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2557 2604 3042 794
Size: 1763 47 438 3792
Fragments arranged by size:
3792 1763 438 47
BstBI TT'CG_AA
Cuts at: 4844 4844
Size: 6040
BstEII G'GTnAC_C
Cuts at: 1184 2258 1184
Size: 1074 4966
BstXI CCAn_nnnn'nTGG
Cuts at: 2155 2155
Size: 6040
BstYI r'GATC_y
Cuts at: 1972 2937 3370 3737 4122 4139 5591 5677 5689
Size: 965 433 367 385 17 1452 86 12
Cuts at: 5689 6035 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 965 433 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2193 2254 2892 3189 513
Size: 552 1128 61 638 297 3364
Fragments arranged by size:
3364 1128 638 552 297 61
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2440 487
Size: 683 56 1214 4087
Fragments arranged by size:
4087 1214 683 56
BtsI GCAGTG_nn'
Cuts at: 3556 4350 4370 3556
Size: 794 20 5226
Fragments arranged by size:
5226 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6040
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6005
DraI TTT'AAA
Cuts at: 3344 3344
Size: 6040
DraIII CAC_nnn'GTG
Cuts at: 2647 2647
Size: 6040
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5047 809
Size: 507 3731 1802
Fragments arranged by size:
3731 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2026 2080 2520
Size: 54 1425 50 275 18 196 54 440
Cuts at: 2520 2895 2928 3030 3186 3195 3487 4376 6005
Size: 375 33 102 156 9 292 889 1629
Cuts at: 6005 8
Size: 43
Fragments arranged by size:
1629 1425 889 440 375 292 275 196
156 102 54 54 50 43 33 18
9
EagI C'GGCC_G
Cuts at: 1537 1812 3186 3487 1537
Size: 275 1374 301 4090
Fragments arranged by size:
4090 1374 301 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2237 2975 3150 3970 1353
Size: 884 738 175 820 3423
Fragments arranged by size:
3423 884 820 738 175
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2062 2150 2331 4627 5001 5147
Size: 1064 138 473 88 181 2296 374 146
Cuts at: 5147 387
Size: 1280
Fragments arranged by size:
2296 1280 1064 473 374 181 146 138
88
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2285 2861 2894 3233 3275 3299 4103
Size: 393 653 576 33 339 42 24 804
Cuts at: 4103 4700 5487 5755 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 804 787 653 597 576 393 339
268 42 33 24
EcoNI CCTnn'n_nnAGG
Cuts at: 3322 3322
Size: 6040
EcoO109I rG'GnC_Cy
Cuts at: 2296 2725 2296
Size: 429 5611
EcoRI G'AATT_C
Cuts at: 3349 3727 3349
Size: 378 5662
FspI TGC'GCA
Cuts at: 2633 4546 2633
Size: 1913 4127
HaeII r_GCGC'y
Cuts at: 1678 2056 2128 2173 5187 1678
Size: 378 72 45 3014 2531
Fragments arranged by size:
3014 2531 378 72 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2828 2860 3299 3331 4681
Size: 32 135 32 1256 32 439 32 1350
Cuts at: 4681 4713 4755 4787 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1350 1256 439 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3384 3630 669
Size: 988 281 1446 246 3079
Fragments arranged by size:
3079 1446 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6040
HpaI GTT'AAC
Cuts at: 3630 3630
Size: 6040
KpnI G_GTAC'C
Cuts at: 3365 3365
Size: 6040
MfeI C'AATT_G
Cuts at: 3639 3639
Size: 6040
MluI A'CGCG_T
Cuts at: 5916 5916
Size: 6040
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2826 5154 5338 1305
Size: 1521 2328 184 2007
Fragments arranged by size:
2328 2007 1521 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2522 2930 10
Size: 54 1425 343 690 408 3120
Fragments arranged by size:
3120 1425 690 408 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2153 2180 2354 2459 2516 2585 2897
Size: 648 987 27 174 105 57 69 312
Cuts at: 2897 3248 3998 4357 4516 518
Size: 351 750 359 159 2042
Fragments arranged by size:
2042 987 750 648 359 351 312 174
159 105 69 57 27
MspA1I CmG'CkG
Cuts at: 1606 2195 2446 2722 3179 3191 3304 3456 4133
Size: 589 251 276 457 12 113 152 677
Cuts at: 4133 5281 5526 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 589 457 276 251 245
152 113 12
NaeI GCC'GGC
Cuts at: 3034 3034
Size: 6040
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5488
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6040
NgoMIV G'CCGG_C
Cuts at: 3032 3032
Size: 6040
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6040
NotI GC'GGCC_GC
Cuts at: 3186 3487 3186
Size: 301 5739
NspI r_CATG'y
Cuts at: 1132 1229 2179 3289 3398 4943 1132
Size: 97 950 1110 109 1545 2229
Fragments arranged by size:
2229 1545 1110 950 109 97
PasI CC'CwG_GG
Cuts at: 1348 2276 1348
Size: 928 5112
PciI A'CATG_T
Cuts at: 4939 4939
Size: 6040
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2647 1108
Size: 1539 4501
PfoI T'CCnGG_A
Cuts at: 2871 2871
Size: 6040
PmeI GTTT'AAAC
Cuts at: 3344 3344
Size: 6040
PmlI CAC'GTG
Cuts at: 2038 2356 2659 2038
Size: 318 303 5419
Fragments arranged by size:
5419 318 303
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4109 4141 5622 5654 4109
Size: 32 1481 32 4495
Fragments arranged by size:
4495 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2725 2725
Size: 6040
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6040
PsiI TTA'TAA
Cuts at: 3610 3610
Size: 6040
PspOMI G'GGCC_C
Cuts at: 2296 2664 2296
Size: 368 5672
PstI C_TGCA'G
Cuts at: 830 3066 3392 830
Size: 2236 326 3478
Fragments arranged by size:
3478 2236 326
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6040
PvuII CAG'CTG
Cuts at: 3179 3179
Size: 6040
SacII CC_GC'GG
Cuts at: 2196 3192 2196
Size: 996 5044
SalI G'TCGA_C
Cuts at: 1936 3382 1936
Size: 1446 4594
SapI GCTCTTCn'nnn_
Cuts at: 2975 2975
Size: 6040
SbfI CC_TGCA'GG
Cuts at: 3392 3392
Size: 6040
ScaI AGT'ACT
Cuts at: 4288 6000 4288
Size: 1712 4328
SfcI C'TryA_G
Cuts at: 826 1045 3062 3388 4523 5204 5395 826
Size: 219 2017 326 1135 681 191 1471
Fragments arranged by size:
2017 1471 1135 681 326 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3194 3194
Size: 6040
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6040
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6040
SmaI CCC'GGG
Cuts at: 1872 3056 3367 1872
Size: 1184 311 4545
Fragments arranged by size:
4545 1184 311
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4144 5045 5307 820
Size: 197 926 2201 901 262 1553
Fragments arranged by size:
2201 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3831 493
Size: 3338 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6040
SphI G_CATG'C
Cuts at: 1132 3289 3398 1132
Size: 2157 109 3774
Fragments arranged by size:
3774 2157 109
SspI AAT'ATT
Cuts at: 5 52 3964 5
Size: 47 3912 2081
Fragments arranged by size:
3912 2081 47
StuI AGG'CCT
Cuts at: 1517 3204 1517
Size: 1687 4353
StyI C'CwwG_G
Cuts at: 513 1065 3225 3479 513
Size: 552 2160 254 3074
Fragments arranged by size:
3074 2160 552 254
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2360 2921 4067 4084 4237 4422 4867
Size: 1255 780 561 1146 17 153 185 445
Cuts at: 4867 325
Size: 1498
Fragments arranged by size:
1498 1255 1146 780 561 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2094 4286 5998
Size: 274 80 33 51 1443 117 2192 1712
Cuts at: 5998 96
Size: 138
Fragments arranged by size:
2192 1712 1443 274 138 117 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6000 6032 6000
Size: 32 6008
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6040
XbaI T'CTAG_A
Cuts at: 3376 3376
Size: 6040
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6040
XmaI C'CCGG_G
Cuts at: 1870 3054 3365 1870
Size: 1184 311 4545
Fragments arranged by size:
4545 1184 311
XmnI GAAnn'nnTTC
Cuts at: 2363 4169 2363
Size: 1806 4234
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3848 276
Size: 53 83 186 936 2314 2468
Fragments arranged by size:
2468 2314 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacII SalI SapI SbfI ScaI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
BsiWI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI
KasI NarI NruI NsiI PacI PspXI PsrI RsrII
SacI SanDI SexAI SfoI SrfI SwaI XcmI