(Circular) (Six-base) MAPSORT of: pFN21AB8445.seq Check: 5718 from: 1 to: 5608 pFN21AB8445 5608 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2560 2628 2560 Size: 68 5540 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3418 278 Size: 53 83 186 936 1882 2468 Fragments arranged by size: 2468 1882 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2454 2951 1937 Size: 517 497 4594 Fragments arranged by size: 4594 517 497 Acc65I G'GTAC_C Cuts at: 2929 2929 Size: 5608 AclI AA'CG_TT Cuts at: 1563 3735 4108 1563 Size: 2172 373 3063 Fragments arranged by size: 3063 2172 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2754 3671 5055 5323 1632 Size: 1122 917 1384 268 1917 Fragments arranged by size: 1917 1384 1122 917 268 AfeI AGC'GCT Cuts at: 2090 2894 2090 Size: 804 4804 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5411 AflIII A'CryG_T Cuts at: 1203 4507 5484 1203 Size: 3304 977 1327 Fragments arranged by size: 3304 1327 977 AgeI A'CCGG_T Cuts at: 4439 4439 Size: 5608 AhdI GACnn_n'nnGTC Cuts at: 2494 4337 2494 Size: 1843 3765 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5608 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5608 AlwNI CAG_nnn'CTG Cuts at: 2395 3351 4923 2395 Size: 956 1572 3080 Fragments arranged by size: 3080 1572 956 ApaI G_GGCC'C Cuts at: 2014 2438 2014 Size: 424 5184 ApaLI G'TGCA_C Cuts at: 3665 4821 3665 Size: 1156 4452 ApoI r'AATT_y Cuts at: 1493 2917 3143 3295 5404 1493 Size: 1424 226 152 2109 1697 Fragments arranged by size: 2109 1697 1424 226 152 AseI AT'TA_AT Cuts at: 160 1032 4162 160 Size: 872 3130 1606 Fragments arranged by size: 3130 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5608 AvaI C'yCGr_G Cuts at: 1870 1943 2111 2933 1870 Size: 73 168 822 4545 Fragments arranged by size: 4545 822 168 73 BamHI G'GATC_C Cuts at: 2938 3305 2938 Size: 367 5241 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2929 4384 618 Size: 325 217 627 1142 1455 1842 Fragments arranged by size: 1842 1455 1142 627 325 217 BanII G_rGCy'C Cuts at: 1391 2014 2120 2438 2731 2927 1391 Size: 623 106 318 293 196 4072 Fragments arranged by size: 4072 623 318 293 196 106 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2896 928 Size: 971 997 3640 Fragments arranged by size: 3640 997 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3799 3833 1903 Size: 34 1862 34 3678 Fragments arranged by size: 3678 1862 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3500 4710 3500 Size: 1210 4398 BclI T'GATC_A Cuts at: 1548 1548 Size: 5608 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4219 136 Size: 107 122 71 631 605 337 2210 1525 Fragments arranged by size: 2210 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5603 5603 Size: 5608 BlpI GC'TnA_GC Cuts at: 2018 3025 2018 Size: 1007 4601 Bme1580I G_kGCm'C Cuts at: 1790 2014 2438 3669 4825 1790 Size: 224 424 1231 1156 2573 Fragments arranged by size: 2573 1231 1156 424 224 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5608 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2042 2433 3339 Size: 416 53 83 399 24 625 391 906 Cuts at: 3339 4297 442 Size: 958 1753 Fragments arranged by size: 1753 958 906 625 416 399 391 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5608 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2281 2394 2417 2690 4268 1239 Size: 1042 113 23 273 1578 2579 Fragments arranged by size: 2579 1578 1042 273 113 23 Bpu10I CC'TnA_GC Cuts at: 2664 2664 Size: 5608 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3733 4598 4896 3733 Size: 865 298 4445 Fragments arranged by size: 4445 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4271 882 Size: 617 2772 2219 Fragments arranged by size: 2772 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2875 3399 493 Size: 711 1671 524 2702 Fragments arranged by size: 2702 1671 711 524 BsaBI GATnn'nnATC Cuts at: 5602 5602 Size: 5608 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3415 3797 4432 275 Size: 53 83 186 936 1882 382 635 1451 Fragments arranged by size: 1882 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4041 4439 4713 4860 5271 4041 Size: 398 274 147 411 4378 Fragments arranged by size: 4378 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5383 Fragments arranged by size: 5383 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2783 2816 2819 1186 Size: 1597 33 3 3975 Fragments arranged by size: 3975 1597 33 3 BseYI C'CCAG_C Cuts at: 2013 2205 4811 5307 2013 Size: 192 2606 496 2314 Fragments arranged by size: 2606 2314 496 192 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2679 1163 Size: 573 943 4092 Fragments arranged by size: 4092 943 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2152 3058 3819 4847 1540 Size: 275 186 151 906 761 1028 2301 Fragments arranged by size: 2301 1028 906 761 275 186 151 BsiHKAI G_wGCw'C Cuts at: 2043 3669 3754 4825 2043 Size: 1626 85 1071 2826 Fragments arranged by size: 2826 1626 1071 85 BsmI GAATG_Cn' Cuts at: 3119 3212 3119 Size: 93 5515 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2014 2043 2120 2438 2731 2927 3669 Size: 399 224 29 77 318 293 196 742 Cuts at: 3669 3754 4825 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 318 293 224 196 85 77 29 BspEI T'CCGG_A Cuts at: 5271 5271 Size: 5608 BspHI T'CATG_A Cuts at: 2073 2586 3497 5227 2073 Size: 513 911 1730 2454 Fragments arranged by size: 2454 1730 911 513 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2560 2628 2963 844 Size: 1716 68 335 3489 Fragments arranged by size: 3489 1716 335 68 BsrBI CCG'CTC Cuts at: 1394 1823 3495 1394 Size: 429 1672 3507 Fragments arranged by size: 3507 1672 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4103 4277 65 Size: 1183 2855 174 1396 Fragments arranged by size: 2855 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4252 4439 1700 Size: 110 2442 187 2869 Fragments arranged by size: 2869 2442 187 110 BsrGI T'GTAC_A Cuts at: 96 2061 96 Size: 1965 3643 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5608 BssSI C'ACGA_G Cuts at: 3668 4680 3668 Size: 1012 4596 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5608 BstBI TT'CG_AA Cuts at: 4412 4412 Size: 5608 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5608 BstYI r'GATC_y Cuts at: 1972 2938 3305 3690 3707 5159 5245 5257 5603 Size: 966 367 385 17 1452 86 12 346 Cuts at: 5603 1972 Size: 1977 Fragments arranged by size: 1977 1452 966 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2886 513 Size: 552 939 882 3235 Fragments arranged by size: 3235 939 882 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2316 487 Size: 683 56 1090 3779 Fragments arranged by size: 3779 1090 683 56 BtsI GCAGTG_nn' Cuts at: 3124 3918 3938 3124 Size: 794 20 4794 Fragments arranged by size: 4794 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5608 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5573 DraI TTT'AAA Cuts at: 2912 2912 Size: 5608 DraIII CAC_nnn'GTG Cuts at: 2611 2613 2611 Size: 2 5606 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2521 4615 809 Size: 507 1205 2094 1802 Fragments arranged by size: 2094 1802 1205 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2230 2749 3055 Size: 54 1425 50 275 18 400 519 306 Cuts at: 3055 3944 5573 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 519 400 306 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3055 1537 Size: 275 1243 4090 Fragments arranged by size: 4090 1243 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2048 3538 1353 Size: 695 1490 3423 Fragments arranged by size: 3423 1490 695 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4195 4569 4715 387 Size: 1064 138 2606 374 146 1280 Fragments arranged by size: 2606 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2281 2394 2417 2690 2754 3671 4268 Size: 393 649 113 23 273 64 917 597 Cuts at: 4268 5055 5323 1239 Size: 787 268 1524 Fragments arranged by size: 1524 917 787 649 597 393 273 268 113 64 23 EcoO109I rG'GnC_Cy Cuts at: 2010 2434 2435 2593 2010 Size: 424 1 158 5025 Fragments arranged by size: 5025 424 158 1 EcoRI G'AATT_C Cuts at: 2917 3295 2917 Size: 378 5230 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2359 2359 Size: 5608 FspI TGC'GCA Cuts at: 4114 4114 Size: 5608 HaeII r_GCGC'y Cuts at: 1678 2092 2182 2896 4755 1678 Size: 414 90 714 1859 2531 Fragments arranged by size: 2531 1859 714 414 90 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2480 2512 4249 4281 4323 Size: 32 135 32 908 32 1737 32 42 Cuts at: 4323 4355 1373 Size: 32 2626 Fragments arranged by size: 2626 1737 908 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2952 3198 669 Size: 988 281 1014 246 3079 Fragments arranged by size: 3079 1014 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5608 HpaI GTT'AAC Cuts at: 3198 3198 Size: 5608 KpnI G_GTAC'C Cuts at: 2933 2933 Size: 5608 MfeI C'AATT_G Cuts at: 3207 3207 Size: 5608 MluI A'CGCG_T Cuts at: 5484 5484 Size: 5608 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2506 2523 4722 4906 1305 Size: 1201 17 2199 184 2007 Fragments arranged by size: 2199 2007 1201 184 17 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3786 Fragments arranged by size: 3786 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2561 3566 3925 4084 518 Size: 648 1395 1005 359 159 2042 Fragments arranged by size: 2042 1395 1005 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2137 2372 2812 3024 3701 4849 5094 1606 Size: 531 235 440 212 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 531 440 245 235 212 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4117 Fragments arranged by size: 4117 939 552 NdeI CA'TA_TG Cuts at: 387 2034 387 Size: 1647 3961 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5608 NotI GC'GGCC_GC Cuts at: 3055 3055 Size: 5608 NspI r_CATG'y Cuts at: 1132 1229 2407 2966 4511 1132 Size: 97 1178 559 1545 2229 Fragments arranged by size: 2229 1545 1178 559 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5608 PciI A'CATG_T Cuts at: 4507 4507 Size: 5608 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5608 PfoI T'CCnGG_A Cuts at: 2778 2778 Size: 5608 PmeI GTTT'AAAC Cuts at: 2912 2912 Size: 5608 PmlI CAC'GTG Cuts at: 2875 2875 Size: 5608 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3677 3709 5190 5222 3677 Size: 32 1481 32 4063 Fragments arranged by size: 4063 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2593 2593 Size: 5608 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5608 PsiI TTA'TAA Cuts at: 3178 3178 Size: 5608 PspOMI G'GGCC_C Cuts at: 2010 2434 2010 Size: 424 5184 PstI C_TGCA'G Cuts at: 830 2571 2960 830 Size: 1741 389 3478 Fragments arranged by size: 3478 1741 389 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5608 SalI G'TCGA_C Cuts at: 1936 2950 1936 Size: 1014 4594 SapI GCTCTTCn'nnn_ Cuts at: 2048 2048 Size: 5608 SbfI CC_TGCA'GG Cuts at: 2960 2960 Size: 5608 ScaI AGT'ACT Cuts at: 3856 5568 3856 Size: 1712 3896 SexAI A'CCwGG_T Cuts at: 2268 2325 2268 Size: 57 5551 SfcI C'TryA_G Cuts at: 826 1045 2567 2956 4091 4772 4963 826 Size: 219 1522 389 1135 681 191 1471 Fragments arranged by size: 1522 1471 1135 681 389 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5608 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5608 SmaI CCC'GGG Cuts at: 1872 2935 1872 Size: 1063 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2111 3712 4613 4875 820 Size: 197 926 168 1601 901 262 1553 Fragments arranged by size: 1601 1553 926 901 262 197 168 SnaBI TAC'GTA Cuts at: 493 3399 493 Size: 2906 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5608 SphI G_CATG'C Cuts at: 1132 2407 2966 1132 Size: 1275 559 3774 Fragments arranged by size: 3774 1275 559 SspI AAT'ATT Cuts at: 5 52 3532 5 Size: 47 3480 2081 Fragments arranged by size: 3480 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5608 StyI C'CwwG_G Cuts at: 513 1065 2004 3047 513 Size: 552 939 1043 3074 Fragments arranged by size: 3074 1043 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2305 2369 2654 2851 3635 3652 3805 Size: 1255 725 64 285 197 784 17 153 Cuts at: 3805 3990 4435 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 784 725 445 285 197 185 153 64 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2061 3854 5566 Size: 274 80 33 51 1443 84 1793 1712 Cuts at: 5566 96 Size: 138 Fragments arranged by size: 1793 1712 1443 274 138 84 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2826 2858 5568 5600 2826 Size: 32 2710 32 2834 Fragments arranged by size: 2834 2710 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2327 1316 Size: 1011 4597 XbaI T'CTAG_A Cuts at: 2944 2944 Size: 5608 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2446 2446 Size: 5608 XhoI C'TCGA_G Cuts at: 1943 2111 1943 Size: 168 5440 XmaI C'CCGG_G Cuts at: 1870 2933 1870 Size: 1063 4545 XmnI GAAnn'nnTTC Cuts at: 2081 3737 2081 Size: 1656 3952 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3416 276 Size: 53 83 186 936 1882 2468 Fragments arranged by size: 2468 1882 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI PvuII RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI