Restriction map for
pFN21AB8443
(Circular) (Six-base) MAPSORT of: pFN21AB8443.seq Check: 5772 from: 1 to: 6463
pFN21AB8443 6463 bp
With 182 enzymes: SgfI *
August 16, 2010 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4273 278
Size: 53 83 186 936 2737 2468
Fragments arranged by size:
2737 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3806 1937
Size: 1869 4594
Acc65I G'GTAC_C
Cuts at: 3784 3784
Size: 6463
AclI AA'CG_TT
Cuts at: 1563 4590 4963 1563
Size: 3027 373 3063
Fragments arranged by size:
3063 3027 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2285 3333 3461 3501 3575 4526 5910 6178
Size: 653 1048 128 40 74 951 1384 268
Cuts at: 6178 1632
Size: 1917
Fragments arranged by size:
1917 1384 1048 951 653 268 128 74
40
AflII C'TTAA_G
Cuts at: 820 1017 3574 820
Size: 197 2557 3709
Fragments arranged by size:
3709 2557 197
AflIII A'CryG_T
Cuts at: 1203 2858 5362 6339 1203
Size: 1655 2504 977 1327
Fragments arranged by size:
2504 1655 1327 977
AgeI A'CCGG_T
Cuts at: 5294 5294
Size: 6463
AhdI GACnn_n'nnGTC
Cuts at: 5192 5192
Size: 6463
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6463
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6463
AlwNI CAG_nnn'CTG
Cuts at: 2055 2333 4206 5778 2055
Size: 278 1873 1572 2740
Fragments arranged by size:
2740 1873 1572 278
ApaI G_GGCC'C
Cuts at: 2299 2299
Size: 6463
ApaLI G'TGCA_C
Cuts at: 4520 5676 4520
Size: 1156 5307
ApoI r'AATT_y
Cuts at: 1493 2958 3772 3998 4150 6259 1493
Size: 1465 814 226 152 2109 1697
Fragments arranged by size:
2109 1697 1465 814 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5017 160
Size: 872 3985 1606
Fragments arranged by size:
3985 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6463
AvaI C'yCGr_G
Cuts at: 1870 1943 2010 3788 1870
Size: 73 67 1778 4545
Fragments arranged by size:
4545 1778 73 67
BamHI G'GATC_C
Cuts at: 3541 3793 4160 3541
Size: 252 367 5844
Fragments arranged by size:
5844 367 252
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2424 2819 3307 3719 3784
Size: 325 217 627 637 395 488 412 65
Cuts at: 3784 5239 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 637 627 488 412 395 325
217 65
BanII G_rGCy'C
Cuts at: 1391 2189 2287 2299 3782 1391
Size: 798 98 12 1483 4072
Fragments arranged by size:
4072 1483 798 98 12
BbeI G_GCGC'C
Cuts at: 3311 3311
Size: 6463
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3606 928
Size: 971 1707 3785
Fragments arranged by size:
3785 1707 971
BbvCI CC'TCA_GC
Cuts at: 2389 2389
Size: 6463
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4654 4688 1903
Size: 34 2717 34 3678
Fragments arranged by size:
3678 2717 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3414 4355 5565 3414
Size: 941 1210 4312
Fragments arranged by size:
4312 1210 941
BclI T'GATC_A
Cuts at: 1548 2755 1548
Size: 1207 5256
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2282 5074 136
Size: 107 122 71 631 605 610 2792 1525
Fragments arranged by size:
2792 1525 631 610 605 122 107 71
BglII A'GATC_T
Cuts at: 3622 6458 3622
Size: 2836 3627
BlpI GC'TnA_GC
Cuts at: 2031 3880 2031
Size: 1849 4614
Bme1580I G_kGCm'C
Cuts at: 1790 2299 3246 4524 5680 1790
Size: 509 947 1278 1156 2573
Fragments arranged by size:
2573 1278 1156 947 509
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6463
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2077 4194 5152
Size: 416 53 83 399 24 660 2117 958
Cuts at: 5152 442
Size: 1753
Fragments arranged by size:
2117 1753 958 660 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6463
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2134 2152 3385 5123 1239
Size: 895 18 1233 1738 2579
Fragments arranged by size:
2579 1738 1233 895 18
Bpu10I CC'TnA_GC
Cuts at: 2389 2389
Size: 6463
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2342 3189 3414 4588 5453 5751 2342
Size: 847 225 1174 865 298 3054
Fragments arranged by size:
3054 1174 865 847 298 225
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3257 3719 5126 882
Size: 617 1758 462 1407 2219
Fragments arranged by size:
2219 1758 1407 617 462
BsaAI yAC'GTr
Cuts at: 493 1204 4254 493
Size: 711 3050 2702
Fragments arranged by size:
3050 2702 711
BsaBI GATnn'nnATC
Cuts at: 6457 6457
Size: 6463
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3308 4270 4652 5287
Size: 53 83 186 936 1775 962 382 635
Cuts at: 5287 275
Size: 1451
Fragments arranged by size:
1775 1451 962 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4896 5294 5568 5715 6126 4896
Size: 398 274 147 411 5233
Fragments arranged by size:
5233 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6238
Fragments arranged by size:
6238 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2086 2089 2116 2276 1186
Size: 900 3 27 160 5373
Fragments arranged by size:
5373 900 160 27 3
BseYI C'CCAG_C
Cuts at: 2405 2929 3338 3672 3739 5666 6162 2405
Size: 524 409 334 67 1927 496 2706
Fragments arranged by size:
2706 1927 524 496 409 334 67
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3158 1163
Size: 573 1422 4468
Fragments arranged by size:
4468 1422 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3913 4674 5702 1540
Size: 275 186 1912 761 1028 2301
Fragments arranged by size:
2301 1912 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2189 4524 4609 5680 2189
Size: 2335 85 1071 2972
Fragments arranged by size:
2972 2335 1071 85
BsmI GAATG_Cn'
Cuts at: 3974 4067 3974
Size: 93 6370
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2189 2287 2299 3246 3782 4524 4609
Size: 399 399 98 12 947 536 742 85
Cuts at: 4609 5680 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 947 742 536 399 399 98
85 12
BspEI T'CCGG_A
Cuts at: 6126 6126
Size: 6463
BspHI T'CATG_A
Cuts at: 3148 3273 4352 6082 3148
Size: 125 1079 1730 3529
Fragments arranged by size:
3529 1730 1079 125
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3110 3818 844
Size: 2266 708 3489
Fragments arranged by size:
3489 2266 708
BsrBI CCG'CTC
Cuts at: 1394 1823 4350 1394
Size: 429 2527 3507
Fragments arranged by size:
3507 2527 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2601 2635 4958 5132 65
Size: 1183 1353 34 2323 174 1396
Fragments arranged by size:
2323 1396 1353 1183 174 34
BsrFI r'CCGG_y
Cuts at: 1700 1810 5107 5294 1700
Size: 110 3297 187 2869
Fragments arranged by size:
3297 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6463
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6463
BssSI C'ACGA_G
Cuts at: 4523 5535 4523
Size: 1012 5451
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6463
BstBI TT'CG_AA
Cuts at: 5267 5267
Size: 6463
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6463
BstXI CCAn_nnnn'nTGG
Cuts at: 2405 3256 3304 2405
Size: 851 48 5564
Fragments arranged by size:
5564 851 48
BstYI r'GATC_y
Cuts at: 1972 3541 3622 3793 4160 4545 4562 6014 6100
Size: 1569 81 171 367 385 17 1452 86
Cuts at: 6100 6112 6458 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1569 1452 385 367 346 171 86
81 17 12
Bsu36I CC'TnA_GG
Cuts at: 2229 2244 2259 2772 2229
Size: 15 15 513 5920
Fragments arranged by size:
5920 513 15 15
BtgI C'CryG_G
Cuts at: 513 1065 2068 3584 513
Size: 552 1003 1516 3392
Fragments arranged by size:
3392 1516 1003 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5724
Fragments arranged by size:
5724 683 56
BtsI GCAGTG_nn'
Cuts at: 2056 2516 3979 4773 4793 2056
Size: 460 1463 794 20 3726
Fragments arranged by size:
3726 1463 794 460 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6463
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6428
DraI TTT'AAA
Cuts at: 3767 3767
Size: 6463
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3433 5470 809
Size: 507 2117 2037 1802
Fragments arranged by size:
2117 2037 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2335 2435 3910
Size: 54 1425 50 275 18 505 100 1475
Cuts at: 3910 4799 6428 8
Size: 889 1629 43
Fragments arranged by size:
1629 1475 1425 889 505 275 100 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3910 1537
Size: 275 2098 4090
Fragments arranged by size:
4090 2098 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3204 3602 4393 1353
Size: 1851 398 791 3423
Fragments arranged by size:
3423 1851 791 398
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5050 5424 5570 387
Size: 1064 138 3461 374 146 1280
Fragments arranged by size:
3461 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2187 2187
Size: 6463
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2134 2152 2285 3333 3385 3461 3501
Size: 393 502 18 133 1048 52 76 40
Cuts at: 3501 3575 4526 5123 5910 6178 1239
Size: 74 951 597 787 268 1524
Fragments arranged by size:
1524 1048 951 787 597 502 393 268
133 76 74 52 40 18
EcoO109I rG'GnC_Cy
Cuts at: 2296 2585 2296
Size: 289 6174
EcoRI G'AATT_C
Cuts at: 3772 4150 3772
Size: 378 6085
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2578 2578
Size: 6463
FspI TGC'GCA
Cuts at: 4969 4969
Size: 6463
HaeII r_GCGC'y
Cuts at: 1678 3311 3739 5610 1678
Size: 1633 428 1871 2531
Fragments arranged by size:
2531 1871 1633 428
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2186 2218 2315 2347 2413
Size: 32 135 32 614 32 97 32 66
Cuts at: 2413 2445 2744 2776 3614 3646 5104 5136 5178
Size: 32 299 32 838 32 1458 32 42
Cuts at: 5178 5210 1373
Size: 32 2626
Fragments arranged by size:
2626 1458 838 614 299 135 97 66
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3807 4053 669
Size: 988 281 1869 246 3079
Fragments arranged by size:
3079 1869 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6463
HpaI GTT'AAC
Cuts at: 4053 4053
Size: 6463
KasI G'GCGC_C
Cuts at: 3307 3307
Size: 6463
KpnI G_GTAC'C
Cuts at: 3788 3788
Size: 6463
MfeI C'AATT_G
Cuts at: 4062 4062
Size: 6463
MluI A'CGCG_T
Cuts at: 6339 6339
Size: 6463
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1998 5577 5761 1305
Size: 693 3579 184 2007
Fragments arranged by size:
3579 2007 693 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2337 10
Size: 54 1425 343 505 4136
Fragments arranged by size:
4136 1425 505 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4421 4780 4939 518
Size: 648 3255 359 159 2042
Fragments arranged by size:
3255 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3743 3879 4556 5704 5949 1606
Size: 2137 136 677 1148 245 2120
Fragments arranged by size:
2137 2120 1148 677 245 136
NarI GG'CG_CC
Cuts at: 3308 3308
Size: 6463
NcoI C'CATG_G
Cuts at: 513 1065 2068 513
Size: 552 1003 4908
Fragments arranged by size:
4908 1003 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6463
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6463
NotI GC'GGCC_GC
Cuts at: 3910 3910
Size: 6463
NspI r_CATG'y
Cuts at: 1132 1229 2862 3821 5366 1132
Size: 97 1633 959 1545 2229
Fragments arranged by size:
2229 1633 1545 959 97
PasI CC'CwG_GG
Cuts at: 1348 2110 2280 1348
Size: 762 170 5531
Fragments arranged by size:
5531 762 170
PciI A'CATG_T
Cuts at: 2858 5362 2858
Size: 2504 3959
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6463
PmeI GTTT'AAAC
Cuts at: 3767 3767
Size: 6463
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4532 4564 6045 6077 4532
Size: 32 1481 32 4918
Fragments arranged by size:
4918 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2585 2585
Size: 6463
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6463
PsiI TTA'TAA
Cuts at: 3224 4033 3224
Size: 809 5654
PspOMI G'GGCC_C
Cuts at: 2295 2295
Size: 6463
PstI C_TGCA'G
Cuts at: 830 3815 830
Size: 2985 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6463
PvuII CAG'CTG
Cuts at: 3743 3743
Size: 6463
SacI G_AGCT'C
Cuts at: 2189 2189
Size: 6463
SalI G'TCGA_C
Cuts at: 1936 3805 1936
Size: 1869 4594
SbfI CC_TGCA'GG
Cuts at: 3815 3815
Size: 6463
ScaI AGT'ACT
Cuts at: 4711 6423 4711
Size: 1712 4751
SfcI C'TryA_G
Cuts at: 826 1045 2748 3811 4946 5627 5818 826
Size: 219 1703 1063 1135 681 191 1471
Fragments arranged by size:
1703 1471 1135 1063 681 219 191
SfoI GGC'GCC
Cuts at: 3309 3309
Size: 6463
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6463
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6463
SmaI CCC'GGG
Cuts at: 1872 3790 1872
Size: 1918 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2321 3204 3429 3574 4567 5468
Size: 197 926 378 883 225 145 993 901
Cuts at: 5468 5730 820
Size: 262 1553
Fragments arranged by size:
1553 993 926 901 883 378 262 225
197 145
SnaBI TAC'GTA
Cuts at: 493 4254 493
Size: 3761 2702
SpeI A'CTAG_T
Cuts at: 152 3683 152
Size: 3531 2932
SphI G_CATG'C
Cuts at: 1132 3821 1132
Size: 2689 3774
SspI AAT'ATT
Cuts at: 5 52 2501 4387 5
Size: 47 2449 1886 2081
Fragments arranged by size:
2449 2081 1886 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6463
StyI C'CwwG_G
Cuts at: 513 1065 2068 2089 3902 513
Size: 552 1003 21 1813 3074
Fragments arranged by size:
3074 1813 1003 552 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4490 4507 4660 4845 5290 325
Size: 1255 2910 17 153 185 445 1498
Fragments arranged by size:
2910 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4709 6421 96
Size: 274 80 33 51 1443 2732 1712 138
Fragments arranged by size:
2732 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2401 2433 6423 6455 2401
Size: 32 3990 32 2409
Fragments arranged by size:
3990 2409 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2200 2329 3518 1316
Size: 884 129 1189 4261
Fragments arranged by size:
4261 1189 884 129
XbaI T'CTAG_A
Cuts at: 3799 3799
Size: 6463
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6463
XmaI C'CCGG_G
Cuts at: 1870 3788 1870
Size: 1918 4545
XmnI GAAnn'nnTTC
Cuts at: 4592 4592
Size: 6463
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4271 276
Size: 53 83 186 936 2737 2468
Fragments arranged by size:
2737 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FseI FspAI
NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI
PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI
SwaI XcmI