Restriction map for
pFN21AB8440
(Circular) (Six-base) MAPSORT of: pFN21AB8440.seq Check: 7960 from: 1 to: 5968
pFN21AB8440 5968 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2717 2717
Size: 5968
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3778 278
Size: 53 83 186 936 2242 2468
Fragments arranged by size:
2468 2242 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3311 1937
Size: 1374 4594
Acc65I G'GTAC_C
Cuts at: 2211 3289 2211
Size: 1078 4890
AclI AA'CG_TT
Cuts at: 1563 4095 4468 1563
Size: 2532 373 3063
Fragments arranged by size:
3063 2532 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4031 5415 5683 1632
Size: 2399 1384 268 1917
Fragments arranged by size:
2399 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5771
AflIII A'CryG_T
Cuts at: 1203 2075 2219 2602 4867 5844 1203
Size: 872 144 383 2265 977 1327
Fragments arranged by size:
2265 1327 977 872 383 144
AgeI A'CCGG_T
Cuts at: 4799 4799
Size: 5968
AhdI GACnn_n'nnGTC
Cuts at: 4697 4697
Size: 5968
AleI CACnn'nnGTG
Cuts at: 1166 2220 1166
Size: 1054 4914
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5968
AlwNI CAG_nnn'CTG
Cuts at: 3711 5283 3711
Size: 1572 4396
ApaLI G'TGCA_C
Cuts at: 4025 5181 4025
Size: 1156 4812
ApoI r'AATT_y
Cuts at: 1493 2239 3277 3503 3655 5764 1493
Size: 746 1038 226 152 2109 1697
Fragments arranged by size:
2109 1697 1038 746 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4522 160
Size: 872 3490 1606
Fragments arranged by size:
3490 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5968
AvaI C'yCGr_G
Cuts at: 1870 1943 3293 1870
Size: 73 1350 4545
Fragments arranged by size:
4545 1350 73
BamHI G'GATC_C
Cuts at: 3298 3665 3298
Size: 367 5601
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2211 2356 3289 4744 618
Size: 325 217 627 424 145 933 1455 1842
Fragments arranged by size:
1842 1455 933 627 424 325 217 145
BanII G_rGCy'C
Cuts at: 1391 3246 3287 1391
Size: 1855 41 4072
Fragments arranged by size:
4072 1855 41
BbeI G_GCGC'C
Cuts at: 2360 2360
Size: 5968
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3203 928
Size: 971 1304 3693
Fragments arranged by size:
3693 1304 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4159 4193 1903
Size: 34 2222 34 3678
Fragments arranged by size:
3678 2222 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2697 3860 5070 2697
Size: 1163 1210 3595
Fragments arranged by size:
3595 1210 1163
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5968
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4579 136
Size: 107 122 71 631 605 2907 1525
Fragments arranged by size:
2907 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2187 5963 2187
Size: 3776 2192
BlpI GC'TnA_GC
Cuts at: 3385 3385
Size: 5968
Bme1580I G_kGCm'C
Cuts at: 1790 2806 4029 5185 1790
Size: 1016 1223 1156 2573
Fragments arranged by size:
2573 1223 1156 1016
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5968
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2635 3699 4657
Size: 416 53 83 399 24 1218 1064 958
Cuts at: 4657 442
Size: 1753
Fragments arranged by size:
1753 1218 1064 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5968
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2168 2320 3057 4628 1239
Size: 929 152 737 1571 2579
Fragments arranged by size:
2579 1571 929 737 152
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4093 4958 5256 4093
Size: 865 298 4805
Fragments arranged by size:
4805 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3227 4631 882
Size: 617 1728 1404 2219
Fragments arranged by size:
2219 1728 1404 617
BsaAI yAC'GTr
Cuts at: 493 1204 2076 2196 2603 3759 493
Size: 711 872 120 407 1156 2702
Fragments arranged by size:
2702 1156 872 711 407 120
BsaBI GATnn'nnATC
Cuts at: 5962 5962
Size: 5968
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2357 3775 4157 4792
Size: 53 83 186 936 824 1418 382 635
Cuts at: 4792 275
Size: 1451
Fragments arranged by size:
1451 1418 936 824 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4401 4799 5073 5220 5631 4401
Size: 398 274 147 411 4738
Fragments arranged by size:
4738 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5743
Fragments arranged by size:
5743 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5968
BseYI C'CCAG_C
Cuts at: 2535 2547 2787 3030 5171 5667 2535
Size: 12 240 243 2141 496 2836
Fragments arranged by size:
2836 2141 496 243 240 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2133 1163
Size: 573 397 4998
Fragments arranged by size:
4998 573 397
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2930 3418 4179 5207 1540
Size: 275 186 929 488 761 1028 2301
Fragments arranged by size:
2301 1028 929 761 488 275 186
BsiHKAI G_wGCw'C
Cuts at: 2256 3246 4029 4114 5185 2256
Size: 990 783 85 1071 3039
Fragments arranged by size:
3039 1071 990 783 85
BsmI GAATG_Cn'
Cuts at: 3479 3572 3479
Size: 93 5875
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2256 2806 3246 3287 4029 4114 5185
Size: 399 466 550 440 41 742 85 1071
Cuts at: 5185 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 550 466 440 399 85
41
BspEI T'CCGG_A
Cuts at: 5631 5631
Size: 5968
BspHI T'CATG_A
Cuts at: 2395 3857 5587 2395
Size: 1462 1730 2776
Fragments arranged by size:
2776 1730 1462
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2717 3323 844
Size: 1873 606 3489
Fragments arranged by size:
3489 1873 606
BsrBI CCG'CTC
Cuts at: 1394 1823 3855 1394
Size: 429 2032 3507
Fragments arranged by size:
3507 2032 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2430 4463 4637 65
Size: 1183 1182 2033 174 1396
Fragments arranged by size:
2033 1396 1183 1182 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2543 3022 4612 4799 1700
Size: 110 733 479 1590 187 2869
Fragments arranged by size:
2869 1590 733 479 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5968
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5968
BssSI C'ACGA_G
Cuts at: 2438 4028 5040 2438
Size: 1590 1012 3366
Fragments arranged by size:
3366 1590 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5968
BstBI TT'CG_AA
Cuts at: 4772 4772
Size: 5968
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5968
BstXI CCAn_nnnn'nTGG
Cuts at: 2035 2222 2035
Size: 187 5781
BstYI r'GATC_y
Cuts at: 1972 2187 2608 3298 3665 4050 4067 5519 5605
Size: 215 421 690 367 385 17 1452 86
Cuts at: 5605 5617 5963 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 690 421 385 367 346 215
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2052 513
Size: 552 939 48 4429
Fragments arranged by size:
4429 939 552 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5229
Fragments arranged by size:
5229 683 56
BtsI GCAGTG_nn'
Cuts at: 2052 2256 2266 3484 4278 4298 2052
Size: 204 10 1218 794 20 3722
Fragments arranged by size:
3722 1218 794 204 20 10
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5968
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5933
DraI TTT'AAA
Cuts at: 3272 3272
Size: 5968
DraIII CAC_nnn'GTG
Cuts at: 2120 2120
Size: 5968
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4975 809
Size: 507 3659 1802
Fragments arranged by size:
3659 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2541 2832 2901
Size: 54 1425 50 275 18 711 291 69
Cuts at: 2901 3415 4304 5933 8
Size: 514 889 1629 43
Fragments arranged by size:
1629 1425 889 711 514 291 275 69
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3415 1537
Size: 275 1603 4090
Fragments arranged by size:
4090 1603 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2556 3898 1353
Size: 1203 1342 3423
Fragments arranged by size:
3423 1342 1203
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4555 4929 5075 387
Size: 1064 138 2966 374 146 1280
Fragments arranged by size:
2966 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3244 3244
Size: 5968
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2168 2320 3057 4031 4628 5415 5683
Size: 393 536 152 737 974 597 787 268
Cuts at: 5683 1239
Size: 1524
Fragments arranged by size:
1524 974 787 737 597 536 393 268
152
EcoO109I rG'GnC_Cy
Cuts at: 2160 2160
Size: 5968
EcoRI G'AATT_C
Cuts at: 2239 3277 3655 2239
Size: 1038 378 4552
Fragments arranged by size:
4552 1038 378
FspI TGC'GCA
Cuts at: 4474 4474
Size: 5968
HaeII r_GCGC'y
Cuts at: 1678 2360 2473 2763 2818 3166 5115 1678
Size: 682 113 290 55 348 1949 2531
Fragments arranged by size:
2531 1949 682 348 290 113 55
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2238 2270 2437 2469 4609
Size: 32 135 32 666 32 167 32 2140
Cuts at: 4609 4641 4683 4715 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2140 666 167 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3312 3558 669
Size: 988 281 1374 246 3079
Fragments arranged by size:
3079 1374 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5968
HpaI GTT'AAC
Cuts at: 3558 3558
Size: 5968
KasI G'GCGC_C
Cuts at: 2356 2356
Size: 5968
KpnI G_GTAC'C
Cuts at: 2215 3293 2215
Size: 1078 4890
MfeI C'AATT_G
Cuts at: 3567 3567
Size: 5968
MluI A'CGCG_T
Cuts at: 5844 5844
Size: 5968
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3013 3231 5082 5266 1305
Size: 1708 218 1851 184 2007
Fragments arranged by size:
2007 1851 1708 218 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4146
Fragments arranged by size:
4146 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2220 3926 4285 4444 518
Size: 648 1054 1706 359 159 2042
Fragments arranged by size:
2042 1706 1054 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2724 3384 4061 5209 5454 1606
Size: 1118 660 677 1148 245 2120
Fragments arranged by size:
2120 1148 1118 677 660 245
NaeI GCC'GGC
Cuts at: 2545 2545
Size: 5968
NarI GG'CG_CC
Cuts at: 2357 2357
Size: 5968
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4477
Fragments arranged by size:
4477 939 552
NdeI CA'TA_TG
Cuts at: 387 2332 387
Size: 1945 4023
NgoMIV G'CCGG_C
Cuts at: 2543 2543
Size: 5968
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5968
NotI GC'GGCC_GC
Cuts at: 3415 3415
Size: 5968
NspI r_CATG'y
Cuts at: 1132 1229 2223 3326 4871 1132
Size: 97 994 1103 1545 2229
Fragments arranged by size:
2229 1545 1103 994 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5968
PciI A'CATG_T
Cuts at: 2219 4867 2219
Size: 2648 3320
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2120 1108
Size: 1012 4956
PmeI GTTT'AAAC
Cuts at: 3272 3272
Size: 5968
PmlI CAC'GTG
Cuts at: 2076 2603 2076
Size: 527 5441
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4037 4069 5550 5582 4037
Size: 32 1481 32 4423
Fragments arranged by size:
4423 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5968
PsiI TTA'TAA
Cuts at: 3538 3538
Size: 5968
PstI C_TGCA'G
Cuts at: 830 3320 830
Size: 2490 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5968
SacI G_AGCT'C
Cuts at: 3246 3246
Size: 5968
SalI G'TCGA_C
Cuts at: 1936 3310 1936
Size: 1374 4594
SbfI CC_TGCA'GG
Cuts at: 3320 3320
Size: 5968
ScaI AGT'ACT
Cuts at: 4216 5928 4216
Size: 1712 4256
SfcI C'TryA_G
Cuts at: 826 1045 2691 3167 3316 4451 5132 5323 826
Size: 219 1646 476 149 1135 681 191 1471
Fragments arranged by size:
1646 1471 1135 681 476 219 191 149
SfoI GGC'GCC
Cuts at: 2358 2358
Size: 5968
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5968
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5968
SmaI CCC'GGG
Cuts at: 1872 3295 1872
Size: 1423 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4072 4973 5235 820
Size: 197 926 2129 901 262 1553
Fragments arranged by size:
2129 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3759 493
Size: 3266 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5968
SphI G_CATG'C
Cuts at: 1132 3326 1132
Size: 2194 3774
SspI AAT'ATT
Cuts at: 5 52 3892 5
Size: 47 3840 2081
Fragments arranged by size:
3840 2081 47
StuI AGG'CCT
Cuts at: 1517 2511 1517
Size: 994 4974
StyI C'CwwG_G
Cuts at: 513 1065 2004 2978 3142 3407 513
Size: 552 939 974 164 265 3074
Fragments arranged by size:
3074 974 939 552 265 164
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2239 2527 3995 4012 4165 4350 4795
Size: 1255 659 288 1468 17 153 185 445
Cuts at: 4795 325
Size: 1498
Fragments arranged by size:
1498 1468 1255 659 445 288 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4214 5926 96
Size: 274 80 33 51 1443 2237 1712 138
Fragments arranged by size:
2237 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3121 3153 5928 5960 3121
Size: 32 2775 32 3129
Fragments arranged by size:
3129 2775 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5968
XbaI T'CTAG_A
Cuts at: 3304 3304
Size: 5968
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2648 2648
Size: 5968
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5968
XmaI C'CCGG_G
Cuts at: 1870 3293 1870
Size: 1423 4545
XmnI GAAnn'nnTTC
Cuts at: 4097 4097
Size: 5968
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3776 276
Size: 53 83 186 936 2242 2468
Fragments arranged by size:
2468 2242 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PstI PvuI SacI SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV
FalI FseI FspAI NruI NsiI PacI PfoI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI
SexAI SfiI SrfI SwaI