Restriction map for
pFN21AB8437
(Circular) (Six-base) MAPSORT of: pFN21AB8437.seq Check: 86 from: 1 to: 6283
pFN21AB8437 6283 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4093 278
Size: 53 83 186 936 2557 2468
Fragments arranged by size:
2557 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3626 1937
Size: 1689 4594
Acc65I G'GTAC_C
Cuts at: 3604 3604
Size: 6283
AclI AA'CG_TT
Cuts at: 1563 4410 4783 1563
Size: 2847 373 3063
Fragments arranged by size:
3063 2847 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2176 3047 3229 4346 5730 5998 1632
Size: 544 871 182 1117 1384 268 1917
Fragments arranged by size:
1917 1384 1117 871 544 268 182
AfeI AGC'GCT
Cuts at: 2189 2210 2189
Size: 21 6262
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6086
AflIII A'CryG_T
Cuts at: 1203 2590 5182 6159 1203
Size: 1387 2592 977 1327
Fragments arranged by size:
2592 1387 1327 977
AgeI A'CCGG_T
Cuts at: 5114 5114
Size: 6283
AhdI GACnn_n'nnGTC
Cuts at: 3478 5012 3478
Size: 1534 4749
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6283
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6283
AlwNI CAG_nnn'CTG
Cuts at: 4026 5598 4026
Size: 1572 4711
ApaLI G'TGCA_C
Cuts at: 4340 5496 4340
Size: 1156 5127
ApoI r'AATT_y
Cuts at: 1493 2499 2764 3592 3818 3970 6079 1493
Size: 1006 265 828 226 152 2109 1697
Fragments arranged by size:
2109 1697 1006 828 265 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4837 160
Size: 872 3805 1606
Fragments arranged by size:
3805 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6283
AvaI C'yCGr_G
Cuts at: 1870 1943 2133 2284 3000 3087 3608 1870
Size: 73 190 151 716 87 521 4545
Fragments arranged by size:
4545 716 521 190 151 87 73
BamHI G'GATC_C
Cuts at: 3266 3613 3980 3266
Size: 347 367 5569
Fragments arranged by size:
5569 367 347
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3604 5059 618
Size: 325 217 627 1817 1455 1842
Fragments arranged by size:
1842 1817 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2544 3602 1391
Size: 1153 1058 4072
Fragments arranged by size:
4072 1153 1058
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2415 2427 3171 928
Size: 971 516 12 744 4040
Fragments arranged by size:
4040 971 744 516 12
BbvCI CC'TCA_GC
Cuts at: 2097 2627 2097
Size: 530 5753
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4474 4508 1903
Size: 34 2537 34 3678
Fragments arranged by size:
3678 2537 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4175 5385 4175
Size: 1210 5073
BclI T'GATC_A
Cuts at: 1548 3081 1548
Size: 1533 4750
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4894 136
Size: 107 122 71 631 605 337 2885 1525
Fragments arranged by size:
2885 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 2755 6278 2755
Size: 3523 2760
BlpI GC'TnA_GC
Cuts at: 3700 3700
Size: 6283
Bme1580I G_kGCm'C
Cuts at: 1790 4344 5500 1790
Size: 2554 1156 2573
Fragments arranged by size:
2573 2554 1156
BmgBI CAC'GTC
Cuts at: 1318 2142 1318
Size: 824 5459
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4014 4972 442
Size: 416 53 83 399 24 2597 958 1753
Fragments arranged by size:
2597 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2150 1056
Size: 1094 5189
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2081 3155 4943 1239
Size: 842 1074 1788 2579
Fragments arranged by size:
2579 1788 1074 842
Bpu10I CC'TnA_GC
Cuts at: 2097 2627 2097
Size: 530 5753
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2845 3473 4408 5273 5571 2845
Size: 628 935 865 298 3557
Fragments arranged by size:
3557 935 865 628 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2315 4946 882
Size: 617 816 2631 2219
Fragments arranged by size:
2631 2219 816 617
BsaAI yAC'GTr
Cuts at: 493 1204 4074 493
Size: 711 2870 2702
Fragments arranged by size:
2870 2702 711
BsaBI GATnn'nnATC
Cuts at: 6277 6277
Size: 6283
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4090 4472 5107 275
Size: 53 83 186 936 2557 382 635 1451
Fragments arranged by size:
2557 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4716 5114 5388 5535 5946 4716
Size: 398 274 147 411 5053
Fragments arranged by size:
5053 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6058
Fragments arranged by size:
6058 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2114 1186
Size: 928 5355
BseYI C'CCAG_C
Cuts at: 3190 3377 5486 5982 3190
Size: 187 2109 496 3491
Fragments arranged by size:
3491 2109 496 187
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5710
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3733 4494 5522 1540
Size: 275 186 1732 761 1028 2301
Fragments arranged by size:
2301 1732 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2291 2544 4344 4429 5500 2291
Size: 253 1800 85 1071 3074
Fragments arranged by size:
3074 1800 1071 253 85
BsmI GAATG_Cn'
Cuts at: 2936 3212 3794 3887 2936
Size: 276 582 93 5332
Fragments arranged by size:
5332 582 276 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2291 2544 3602 4344 4429 5500 1391
Size: 399 501 253 1058 742 85 1071 2174
Fragments arranged by size:
2174 1071 1058 742 501 399 253 85
BspEI T'CCGG_A
Cuts at: 5946 5946
Size: 6283
BspHI T'CATG_A
Cuts at: 2449 4172 5902 2449
Size: 1723 1730 2830
Fragments arranged by size:
2830 1730 1723
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3638 844
Size: 2794 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2950 4170 1394
Size: 429 1127 1220 3507
Fragments arranged by size:
3507 1220 1127 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4778 4952 65
Size: 1183 3530 174 1396
Fragments arranged by size:
3530 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4927 5114 1700
Size: 110 3117 187 2869
Fragments arranged by size:
3117 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6283
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6283
BssSI C'ACGA_G
Cuts at: 2606 3499 4343 5355 2606
Size: 893 844 1012 3534
Fragments arranged by size:
3534 1012 893 844
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6283
BstBI TT'CG_AA
Cuts at: 5087 5087
Size: 6283
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6283
BstXI CCAn_nnnn'nTGG
Cuts at: 2608 2608
Size: 6283
BstYI r'GATC_y
Cuts at: 1972 2755 3266 3613 3980 4365 4382 5834 5920
Size: 783 511 347 367 385 17 1452 86
Cuts at: 5920 5932 6278 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 783 511 385 367 347 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4792
Fragments arranged by size:
4792 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2737 487
Size: 683 56 1511 4033
Fragments arranged by size:
4033 1511 683 56
BtsI GCAGTG_nn'
Cuts at: 2233 3326 3799 4593 4613 2233
Size: 1093 473 794 20 3903
Fragments arranged by size:
3903 1093 794 473 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6283
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6248
DraI TTT'AAA
Cuts at: 3587 3587
Size: 6283
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5290 809
Size: 507 3974 1802
Fragments arranged by size:
3974 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2010 2073 2085
Size: 54 1425 50 275 18 180 63 12
Cuts at: 2085 3730 4619 6248 8
Size: 1645 889 1629 43
Fragments arranged by size:
1645 1629 1425 889 275 180 63 54
50 43 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 3730 1537
Size: 275 1918 4090
Fragments arranged by size:
4090 1918 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2532 3550 4213 1353
Size: 1179 1018 663 3423
Fragments arranged by size:
3423 1179 1018 663
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4870 5244 5390 387
Size: 1064 138 3281 374 146 1280
Fragments arranged by size:
3281 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2542 2542
Size: 6283
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2081 2176 3047 3155 3229 4346 4943
Size: 393 449 95 871 108 74 1117 597
Cuts at: 4943 5730 5998 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1117 871 787 597 449 393 268
108 95 74
EcoRI G'AATT_C
Cuts at: 3592 3970 3592
Size: 378 5905
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2561 2969 3092 2561
Size: 408 123 5752
Fragments arranged by size:
5752 408 123
FspI TGC'GCA
Cuts at: 4789 4789
Size: 6283
HaeII r_GCGC'y
Cuts at: 1678 2191 2212 5430 1678
Size: 513 21 3218 2531
Fragments arranged by size:
3218 2531 513 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3464 3496 4924 4956 4998
Size: 32 135 32 1892 32 1428 32 42
Cuts at: 4998 5030 1373
Size: 32 2626
Fragments arranged by size:
2626 1892 1428 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3627 3873 669
Size: 988 281 1689 246 3079
Fragments arranged by size:
3079 1689 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6283
HpaI GTT'AAC
Cuts at: 3873 3873
Size: 6283
KpnI G_GTAC'C
Cuts at: 3608 3608
Size: 6283
MfeI C'AATT_G
Cuts at: 3882 3882
Size: 6283
MluI A'CGCG_T
Cuts at: 6159 6159
Size: 6283
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5397 5581 1305
Size: 4092 184 2007
Fragments arranged by size:
4092 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2012 10
Size: 54 1425 343 180 4281
Fragments arranged by size:
4281 1425 343 180 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2606 4241 4600 4759 518
Size: 648 1440 1635 359 159 2042
Fragments arranged by size:
2042 1635 1440 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2079 3124 3699 4376 5524 5769 1606
Size: 473 1045 575 677 1148 245 2120
Fragments arranged by size:
2120 1148 1045 677 575 473 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4792
Fragments arranged by size:
4792 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6283
NheI G'CTAG_C
Cuts at: 1052 2146 1052
Size: 1094 5189
NotI GC'GGCC_GC
Cuts at: 3730 3730
Size: 6283
NspI r_CATG'y
Cuts at: 1132 1229 2594 3056 3641 5186 1132
Size: 97 1365 462 585 1545 2229
Fragments arranged by size:
2229 1545 1365 585 462 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6283
PciI A'CATG_T
Cuts at: 2590 5182 2590
Size: 2592 3691
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6283
PfoI T'CCnGG_A
Cuts at: 2706 3171 2706
Size: 465 5818
PmeI GTTT'AAAC
Cuts at: 3587 3587
Size: 6283
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3386 3418 4352 4384 5865 5897 3386
Size: 32 934 32 1481 32 3772
Fragments arranged by size:
3772 1481 934 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2263 1613
Size: 650 5633
PsiI TTA'TAA
Cuts at: 2943 3853 2943
Size: 910 5373
PspXI vC'TCGA_Gb
Cuts at: 2284 3087 2284
Size: 803 5480
PstI C_TGCA'G
Cuts at: 830 2057 2208 2651 3635 830
Size: 1227 151 443 984 3478
Fragments arranged by size:
3478 1227 984 443 151
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6283
PvuII CAG'CTG
Cuts at: 3124 3124
Size: 6283
RsrII CG'GwC_CG
Cuts at: 2081 2081
Size: 6283
SacI G_AGCT'C
Cuts at: 2544 2544
Size: 6283
SalI G'TCGA_C
Cuts at: 1936 3625 1936
Size: 1689 4594
SapI GCTCTTCn'nnn_
Cuts at: 2532 2532
Size: 6283
SbfI CC_TGCA'GG
Cuts at: 3635 3635
Size: 6283
ScaI AGT'ACT
Cuts at: 4531 6243 4531
Size: 1712 4571
SfcI C'TryA_G
Cuts at: 826 1045 2053 2204 2647 2717 3434 3487 3631
Size: 219 1008 151 443 70 717 53 144
Cuts at: 3631 4766 5447 5638 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1008 717 681 443 219 191
151 144 70 53
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6283
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6283
SmaI CCC'GGG
Cuts at: 1872 3610 1872
Size: 1738 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2284 2824 3000 3087 3452 4387
Size: 197 926 341 540 176 87 365 935
Cuts at: 4387 5288 5550 820
Size: 901 262 1553
Fragments arranged by size:
1553 935 926 901 540 365 341 262
197 176 87
SnaBI TAC'GTA
Cuts at: 493 4074 493
Size: 3581 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6283
SphI G_CATG'C
Cuts at: 1132 3641 1132
Size: 2509 3774
SspI AAT'ATT
Cuts at: 5 52 4207 5
Size: 47 4155 2081
Fragments arranged by size:
4155 2081 47
StuI AGG'CCT
Cuts at: 1517 2444 1517
Size: 927 5356
StyI C'CwwG_G
Cuts at: 513 1065 2004 2013 2088 2358 2398 3722 513
Size: 552 939 9 75 270 40 1324 3074
Fragments arranged by size:
3074 1324 939 552 270 75 40 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3113 3182 4310 4327 4480 4665 5110
Size: 1255 1533 69 1128 17 153 185 445
Cuts at: 5110 325
Size: 1498
Fragments arranged by size:
1533 1498 1255 1128 445 185 153 69
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4529 6241 96
Size: 274 80 33 51 1443 2552 1712 138
Fragments arranged by size:
2552 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6243 6275 6243
Size: 32 6251
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6283
XbaI T'CTAG_A
Cuts at: 3619 3619
Size: 6283
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2674 2674
Size: 6283
XhoI C'TCGA_G
Cuts at: 1943 2284 3000 3087 1943
Size: 341 716 87 5139
Fragments arranged by size:
5139 716 341 87
XmaI C'CCGG_G
Cuts at: 1870 3608 1870
Size: 1738 4545
XmnI GAAnn'nnTTC
Cuts at: 4412 4412
Size: 6283
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4091 276
Size: 53 83 186 936 2557 2468
Fragments arranged by size:
2557 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspXI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PsrI SacII SanDI
SexAI SfiI SfoI SrfI SwaI