Restriction map for
pFN21AB8436
(Circular) (Six-base) MAPSORT of: pFN21AB8436.seq Check: 4876 from: 1 to: 5782
pFN21AB8436 5782 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3592 278
Size: 53 83 186 936 2056 2468
Fragments arranged by size:
2468 2056 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2871 3125 1937
Size: 934 254 4594
Fragments arranged by size:
4594 934 254
Acc65I G'GTAC_C
Cuts at: 3103 3103
Size: 5782
AclI AA'CG_TT
Cuts at: 1563 3909 4282 1563
Size: 2346 373 3063
Fragments arranged by size:
3063 2346 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2954 3083 3845 5229 5497 1632
Size: 1322 129 762 1384 268 1917
Fragments arranged by size:
1917 1384 1322 762 268 129
AfeI AGC'GCT
Cuts at: 2697 2697
Size: 5782
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5585
AflIII A'CryG_T
Cuts at: 1203 4681 5658 1203
Size: 3478 977 1327
Fragments arranged by size:
3478 1327 977
AgeI A'CCGG_T
Cuts at: 4613 4613
Size: 5782
AhdI GACnn_n'nnGTC
Cuts at: 4511 4511
Size: 5782
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5782
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5782
AlwNI CAG_nnn'CTG
Cuts at: 3525 5097 3525
Size: 1572 4210
ApaLI G'TGCA_C
Cuts at: 3839 4995 3839
Size: 1156 4626
ApoI r'AATT_y
Cuts at: 1493 2924 3091 3317 3469 5578 1493
Size: 1431 167 226 152 2109 1697
Fragments arranged by size:
2109 1697 1431 226 167 152
AseI AT'TA_AT
Cuts at: 160 1032 4336 160
Size: 872 3304 1606
Fragments arranged by size:
3304 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5782
AvaI C'yCGr_G
Cuts at: 1870 1943 2138 2919 3107 1870
Size: 73 195 781 188 4545
Fragments arranged by size:
4545 781 195 188 73
BamHI G'GATC_C
Cuts at: 3112 3479 3112
Size: 367 5415
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3103 4558 618
Size: 325 217 627 1316 1455 1842
Fragments arranged by size:
1842 1455 1316 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2124 3101 1391
Size: 733 977 4072
Fragments arranged by size:
4072 977 733
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4811
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3973 4007 1903
Size: 34 2036 34 3678
Fragments arranged by size:
3678 2036 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2195 2646 3674 4884 2195
Size: 451 1028 1210 3093
Fragments arranged by size:
3093 1210 1028 451
BclI T'GATC_A
Cuts at: 1548 2274 2710 1548
Size: 726 436 4620
Fragments arranged by size:
4620 726 436
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2267 4393 136
Size: 107 122 71 631 605 595 2126 1525
Fragments arranged by size:
2126 1525 631 605 595 122 107 71
BglII A'GATC_T
Cuts at: 2833 5777 2833
Size: 2944 2838
BlpI GC'TnA_GC
Cuts at: 3199 3199
Size: 5782
Bme1580I G_kGCm'C
Cuts at: 1790 3843 4999 1790
Size: 2053 1156 2573
Fragments arranged by size:
2573 2053 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5782
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3513 4471 442
Size: 416 53 83 399 24 2096 958 1753
Fragments arranged by size:
2096 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5782
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2033 3035 4442 1239
Size: 794 1002 1407 2579
Fragments arranged by size:
2579 1407 1002 794
Bpu10I CC'TnA_GC
Cuts at: 2036 2036
Size: 5782
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2108 2279 3907 4772 5070 2108
Size: 171 1628 865 298 2820
Fragments arranged by size:
2820 1628 865 298 171
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4445 882
Size: 617 2946 2219
Fragments arranged by size:
2946 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2991 3573 493
Size: 711 1787 582 2702
Fragments arranged by size:
2702 1787 711 582
BsaBI GATnn'nnATC
Cuts at: 2183 5776 2183
Size: 3593 2189
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2109 3589 3971 4606
Size: 53 83 186 936 576 1480 382 635
Cuts at: 4606 275
Size: 1451
Fragments arranged by size:
1480 1451 936 635 576 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4215 4613 4887 5034 5445 4215
Size: 398 274 147 411 4552
Fragments arranged by size:
4552 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2351 2381 1178
Size: 30 165 30 948 30 4579
Fragments arranged by size:
4579 948 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2057 2794 2858 1186
Size: 871 737 64 4110
Fragments arranged by size:
4110 871 737 64
BseYI C'CCAG_C
Cuts at: 4985 5481 4985
Size: 496 5286
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5209
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3232 3993 5021 1540
Size: 275 186 1231 761 1028 2301
Fragments arranged by size:
2301 1231 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2124 2319 3843 3928 4999 2124
Size: 195 1524 85 1071 2907
Fragments arranged by size:
2907 1524 1071 195 85
BsmI GAATG_Cn'
Cuts at: 2438 3293 3386 2438
Size: 855 93 4834
Fragments arranged by size:
4834 855 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2124 2319 3101 3843 3928 4999 1391
Size: 399 334 195 782 742 85 1071 2174
Fragments arranged by size:
2174 1071 782 742 399 334 195 85
BspEI T'CCGG_A
Cuts at: 5445 5445
Size: 5782
BspHI T'CATG_A
Cuts at: 3671 5401 3671
Size: 1730 4052
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2529 3137 844
Size: 1685 608 3489
Fragments arranged by size:
3489 1685 608
BsrBI CCG'CTC
Cuts at: 1394 1823 2083 3669 1394
Size: 429 260 1586 3507
Fragments arranged by size:
3507 1586 429 260
BsrDI GCAATG_nn'
Cuts at: 65 1248 4277 4451 65
Size: 1183 3029 174 1396
Fragments arranged by size:
3029 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4426 4613 1700
Size: 110 2616 187 2869
Fragments arranged by size:
2869 2616 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5782
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5782
BssSI C'ACGA_G
Cuts at: 2720 3842 4854 2720
Size: 1122 1012 3648
Fragments arranged by size:
3648 1122 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5782
BstBI TT'CG_AA
Cuts at: 4586 4586
Size: 5782
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5782
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5782
BstYI r'GATC_y
Cuts at: 1972 2298 2833 3112 3479 3864 3881 5333 5419
Size: 326 535 279 367 385 17 1452 86
Cuts at: 5419 5431 5777 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 535 385 367 346 326 279
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2191 2283 513
Size: 552 1126 92 4012
Fragments arranged by size:
4012 1126 552 92
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5043
Fragments arranged by size:
5043 683 56
BtsI GCAGTG_nn'
Cuts at: 2656 3298 4092 4112 2656
Size: 642 794 20 4326
Fragments arranged by size:
4326 794 642 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5782
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5747
DraI TTT'AAA
Cuts at: 3086 3086
Size: 5782
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2014 4789 809
Size: 507 698 2775 1802
Fragments arranged by size:
2775 1802 698 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2259 3229 4118
Size: 54 1425 50 275 18 429 970 889
Cuts at: 4118 5747 8
Size: 1629 43
Fragments arranged by size:
1629 1425 970 889 429 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3229 1537
Size: 275 1417 4090
Fragments arranged by size:
4090 1417 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3712 1353
Size: 2359 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2077 4369 4743 4889 387
Size: 1064 138 488 2292 374 146 1280
Fragments arranged by size:
2292 1280 1064 488 374 146 138
EcoICRI GAG'CTC
Cuts at: 2122 2122
Size: 5782
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2033 2954 3035 3083 3845 4442 5229
Size: 393 401 921 81 48 762 597 787
Cuts at: 5229 5497 1239
Size: 268 1524
Fragments arranged by size:
1524 921 787 762 597 401 393 268
81 48
EcoNI CCTnn'n_nnAGG
Cuts at: 2039 2039
Size: 5782
EcoRI G'AATT_C
Cuts at: 2924 3091 3469 2924
Size: 167 378 5237
Fragments arranged by size:
5237 378 167
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2558 2782 2558
Size: 224 5558
FspI TGC'GCA
Cuts at: 4288 4288
Size: 5782
HaeII r_GCGC'y
Cuts at: 1678 2699 4929 1678
Size: 1021 2230 2531
Fragments arranged by size:
2531 2230 1021
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2351 2383 2538 2570 4423
Size: 32 135 32 779 32 155 32 1853
Cuts at: 4423 4455 4497 4529 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1853 779 155 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2872 3126 3372 669
Size: 988 281 934 254 246 3079
Fragments arranged by size:
3079 988 934 281 254 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5782
HpaI GTT'AAC
Cuts at: 3372 3372
Size: 5782
KpnI G_GTAC'C
Cuts at: 3107 3107
Size: 5782
MfeI C'AATT_G
Cuts at: 3381 3381
Size: 5782
MluI A'CGCG_T
Cuts at: 5658 5658
Size: 5782
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4896 5080 1305
Size: 3591 184 2007
Fragments arranged by size:
3591 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3960
Fragments arranged by size:
3960 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2261 3740 4099 4258 518
Size: 648 1095 1479 359 159 2042
Fragments arranged by size:
2042 1479 1095 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3198 3875 5023 5268 1606
Size: 1592 677 1148 245 2120
Fragments arranged by size:
2120 1592 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2191 2283 513
Size: 552 1126 92 4012
Fragments arranged by size:
4012 1126 552 92
NdeI CA'TA_TG
Cuts at: 387 2386 387
Size: 1999 3783
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5782
NotI GC'GGCC_GC
Cuts at: 3229 3229
Size: 5782
NspI r_CATG'y
Cuts at: 1132 1229 3140 4685 1132
Size: 97 1911 1545 2229
Fragments arranged by size:
2229 1911 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5782
PciI A'CATG_T
Cuts at: 4681 4681
Size: 5782
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5782
PfoI T'CCnGG_A
Cuts at: 2419 2427 2419
Size: 8 5774
PmeI GTTT'AAAC
Cuts at: 3086 3086
Size: 5782
PmlI CAC'GTG
Cuts at: 2991 2991
Size: 5782
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3851 3883 5364 5396 3851
Size: 32 1481 32 4237
Fragments arranged by size:
4237 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5782
PsiI TTA'TAA
Cuts at: 3352 3352
Size: 5782
PstI C_TGCA'G
Cuts at: 830 3134 830
Size: 2304 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5782
SacI G_AGCT'C
Cuts at: 2124 2124
Size: 5782
SalI G'TCGA_C
Cuts at: 1936 2870 3124 1936
Size: 934 254 4594
Fragments arranged by size:
4594 934 254
SbfI CC_TGCA'GG
Cuts at: 3134 3134
Size: 5782
ScaI AGT'ACT
Cuts at: 4030 5742 4030
Size: 1712 4070
SfcI C'TryA_G
Cuts at: 826 1045 3130 4265 4946 5137 826
Size: 219 2085 1135 681 191 1471
Fragments arranged by size:
2085 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2267 2267
Size: 5782
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5782
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5782
SmaI CCC'GGG
Cuts at: 1872 3109 1872
Size: 1237 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2123 2294 3886 4787 5049 820
Size: 197 926 180 171 1592 901 262 1553
Fragments arranged by size:
1592 1553 926 901 262 197 180 171
SnaBI TAC'GTA
Cuts at: 493 3573 493
Size: 3080 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5782
SphI G_CATG'C
Cuts at: 1132 3140 1132
Size: 2008 3774
SspI AAT'ATT
Cuts at: 5 52 3706 5
Size: 47 3654 2081
Fragments arranged by size:
3654 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5782
StyI C'CwwG_G
Cuts at: 513 1065 2049 2191 2283 3221 513
Size: 552 984 142 92 938 3074
Fragments arranged by size:
3074 984 938 552 142 92
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3809 3826 3979 4164 4609 325
Size: 1255 2229 17 153 185 445 1498
Fragments arranged by size:
2229 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3069 4028 5740
Size: 274 80 33 51 1443 1092 959 1712
Cuts at: 5740 96
Size: 138
Fragments arranged by size:
1712 1443 1092 959 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2283 2315 5742 5774 2283
Size: 32 3427 32 2291
Fragments arranged by size:
3427 2291 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5782
XbaI T'CTAG_A
Cuts at: 2839 3118 2839
Size: 279 5503
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5782
XmaI C'CCGG_G
Cuts at: 1870 3107 1870
Size: 1237 4545
XmnI GAAnn'nnTTC
Cuts at: 2252 2928 2939 3911 2252
Size: 676 11 972 4123
Fragments arranged by size:
4123 972 676 11
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3590 276
Size: 53 83 186 936 2056 2468
Fragments arranged by size:
2468 2056 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PstI PvuI SacI SalI SbfI ScaI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacII SanDI SapI SexAI SfoI SrfI SwaI XcmI