Restriction map for
pFN21AB8432
(Circular) (Six-base) MAPSORT of: pFN21AB8432.seq Check: 383 from: 1 to: 7504
pFN21AB8432 7504 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2678 2678
Size: 7504
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5314 278
Size: 53 83 186 936 3778 2468
Fragments arranged by size:
3778 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3758 4035 4847 1937
Size: 1821 277 812 4594
Fragments arranged by size:
4594 1821 812 277
Acc65I G'GTAC_C
Cuts at: 4825 4825
Size: 7504
AclI AA'CG_TT
Cuts at: 1563 5631 6004 1563
Size: 4068 373 3063
Fragments arranged by size:
4068 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2114 2135 3140 4022 5567 6951 7219 1632
Size: 482 21 1005 882 1545 1384 268 1917
Fragments arranged by size:
1917 1545 1384 1005 882 482 268 21
AfeI AGC'GCT
Cuts at: 2639 3689 2639
Size: 1050 6454
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7307
AflIII A'CryG_T
Cuts at: 1203 2745 4610 6403 7380 1203
Size: 1542 1865 1793 977 1327
Fragments arranged by size:
1865 1793 1542 1327 977
AgeI A'CCGG_T
Cuts at: 6335 6335
Size: 7504
AhdI GACnn_n'nnGTC
Cuts at: 2357 3841 4134 6233 2357
Size: 1484 293 2099 3628
Fragments arranged by size:
3628 2099 1484 293
AleI CACnn'nnGTG
Cuts at: 1166 2905 1166
Size: 1739 5765
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7504
AlwNI CAG_nnn'CTG
Cuts at: 2158 2332 2459 5247 6819 2158
Size: 174 127 2788 1572 2843
Fragments arranged by size:
2843 2788 1572 174 127
ApaI G_GGCC'C
Cuts at: 2914 2942 3325 2914
Size: 28 383 7093
Fragments arranged by size:
7093 383 28
ApaLI G'TGCA_C
Cuts at: 2165 3805 5561 6717 2165
Size: 1640 1756 1156 2952
Fragments arranged by size:
2952 1756 1640 1156
ApoI r'AATT_y
Cuts at: 1493 4813 5039 5191 7300 1493
Size: 3320 226 152 2109 1697
Fragments arranged by size:
3320 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6058 160
Size: 872 5026 1606
Fragments arranged by size:
5026 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7504
AvaI C'yCGr_G
Cuts at: 1870 1943 2412 2448 2753 3660 4829 1870
Size: 73 469 36 305 907 1169 4545
Fragments arranged by size:
4545 1169 907 469 305 73 36
BamHI G'GATC_C
Cuts at: 2237 4834 5201 2237
Size: 2597 367 4540
Fragments arranged by size:
4540 2597 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2216 2762 4242 4825 6280
Size: 325 217 627 429 546 1480 583 1455
Cuts at: 6280 618
Size: 1842
Fragments arranged by size:
1842 1480 1455 627 583 546 429 325
217
BanII G_rGCy'C
Cuts at: 1391 2468 2914 2942 3070 3325 4260 4653 4692
Size: 1077 446 28 128 255 935 393 39
Cuts at: 4692 4823 1391
Size: 131 4072
Fragments arranged by size:
4072 1077 935 446 393 255 131 128
39 28
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6533
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2744 2778 5695 5729 1903
Size: 34 807 34 2917 34 3678
Fragments arranged by size:
3678 2917 807 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5396 6606 5396
Size: 1210 6294
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7504
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 6115 136
Size: 107 122 71 631 605 337 4106 1525
Fragments arranged by size:
4106 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 4581 7499 4581
Size: 2918 4586
BlpI GC'TnA_GC
Cuts at: 4921 4921
Size: 7504
Bme1580I G_kGCm'C
Cuts at: 1790 2169 2219 2914 2942 3325 3809 4245 5565
Size: 379 50 695 28 383 484 436 1320
Cuts at: 5565 6721 1790
Size: 1156 2573
Fragments arranged by size:
2573 1320 1156 695 484 436 383 379
50 28
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7504
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2046 3568 5235
Size: 416 53 83 399 24 629 1522 1667
Cuts at: 5235 6193 442
Size: 958 1753
Fragments arranged by size:
1753 1667 1522 958 629 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7504
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2455 2455
Size: 7504
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2038 2048 2391 2522 2639 3166 3878 6164
Size: 799 10 343 131 117 527 712 2286
Cuts at: 6164 1239
Size: 2579
Fragments arranged by size:
2579 2286 799 712 527 343 131 117
10
Bpu10I CC'TnA_GC
Cuts at: 2641 2641
Size: 7504
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2861 3054 3149 4462 4712 5629 6494 6792 2861
Size: 193 95 1313 250 917 865 298 3573
Fragments arranged by size:
3573 1313 917 865 298 250 193 95
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2676 4503 6167 882
Size: 617 1177 1827 1664 2219
Fragments arranged by size:
2219 1827 1664 1177 617
BsaAI yAC'GTr
Cuts at: 493 1204 4039 4639 5295 493
Size: 711 2835 600 656 2702
Fragments arranged by size:
2835 2702 711 656 600
BsaBI GATnn'nnATC
Cuts at: 3083 7498 3083
Size: 4415 3089
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5311 5693 6328 275
Size: 53 83 186 936 3778 382 635 1451
Fragments arranged by size:
3778 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2118 2631 4296 4332 5937 6335 6609 6756 7167
Size: 513 1665 36 1605 398 274 147 411
Cuts at: 7167 2118
Size: 2455
Fragments arranged by size:
2455 1665 1605 513 411 398 274 147
36
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7279
Fragments arranged by size:
7279 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3089 3427 1186
Size: 1903 338 5263
Fragments arranged by size:
5263 1903 338
BseYI C'CCAG_C
Cuts at: 2110 3396 3456 3523 4234 4342 4762 6707 7203
Size: 1286 60 67 711 108 420 1945 496
Cuts at: 7203 2110
Size: 2411
Fragments arranged by size:
2411 1945 1286 711 496 420 108 67
60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2239 2902 2948 3257 3392 3584 3869
Size: 573 503 663 46 309 135 192 285
Cuts at: 3869 4097 1163
Size: 228 4570
Fragments arranged by size:
4570 663 573 503 309 285 228 192
135 46
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2119 4954 5715 6743 1540
Size: 275 186 118 2835 761 1028 2301
Fragments arranged by size:
2835 2301 1028 761 275 186 118
BsiHKAI G_wGCw'C
Cuts at: 2169 2825 3070 3809 4260 4284 4692 5565 5650
Size: 656 245 739 451 24 408 873 85
Cuts at: 5650 6721 2169
Size: 1071 2952
Fragments arranged by size:
2952 1071 873 739 656 451 408 245
85 24
BsmI GAATG_Cn'
Cuts at: 3362 5015 5108 3362
Size: 1653 93 5758
Fragments arranged by size:
5758 1653 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3596 4255 4382 3596
Size: 659 127 6718
Fragments arranged by size:
6718 659 127
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2169 2219 2468 2825 2914 2942 3070
Size: 399 379 50 249 357 89 28 128
Cuts at: 3070 3325 3809 4245 4260 4284 4653 4692 4823
Size: 255 484 436 15 24 369 39 131
Cuts at: 4823 5565 5650 6721 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 484 436 399 379 369
357 255 249 131 128 89 85 50
39 28 24 15
BspEI T'CCGG_A
Cuts at: 2631 7167 2631
Size: 4536 2968
BspHI T'CATG_A
Cuts at: 3328 4404 5393 7123 3328
Size: 1076 989 1730 3709
Fragments arranged by size:
3709 1730 1076 989
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2261 2444 2678 3020 3414 3753 3891 4422
Size: 1417 183 234 342 394 339 138 531
Cuts at: 4422 4859 844
Size: 437 3489
Fragments arranged by size:
3489 1417 531 437 394 342 339 234
183 138
BsrBI CCG'CTC
Cuts at: 1394 1823 5391 1394
Size: 429 3568 3507
Fragments arranged by size:
3568 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5999 6173 65
Size: 1183 4751 174 1396
Fragments arranged by size:
4751 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2892 2967 3573 3655 6148 6335 1700
Size: 110 1082 75 606 82 2493 187 2869
Fragments arranged by size:
2869 2493 1082 606 187 110 82 75
BsrGI T'GTAC_A
Cuts at: 96 2742 96
Size: 2646 4858
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7504
BssSI C'ACGA_G
Cuts at: 5564 6576 5564
Size: 1012 6492
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2842 794
Size: 2048 5456
BstBI TT'CG_AA
Cuts at: 3582 6308 3582
Size: 2726 4778
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7504
BstYI r'GATC_y
Cuts at: 1972 2237 4501 4581 4834 5201 5586 5603 7055
Size: 265 2264 80 253 367 385 17 1452
Cuts at: 7055 7141 7153 7499 1972
Size: 86 12 346 1977
Fragments arranged by size:
2264 1977 1452 385 367 346 265 253
86 80 17 12
Bsu36I CC'TnA_GG
Cuts at: 2460 3108 2460
Size: 648 6856
BtgI C'CryG_G
Cuts at: 513 1065 3620 3646 4080 4176 4644 4659 513
Size: 552 2555 26 434 96 468 15 3358
Fragments arranged by size:
3358 2555 552 468 434 96 26 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3993 4145 4270 487
Size: 683 56 2767 152 125 3721
Fragments arranged by size:
3721 2767 683 152 125 56
BtsI GCAGTG_nn'
Cuts at: 3809 4456 5020 5814 5834 3809
Size: 647 564 794 20 5479
Fragments arranged by size:
5479 794 647 564 20
ClaI AT'CG_AT
Cuts at: 1554 2850 1554
Size: 1296 6208
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7469
DraI TTT'AAA
Cuts at: 3211 4808 3211
Size: 1597 5907
DraIII CAC_nnn'GTG
Cuts at: 2842 2842
Size: 7504
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4301 6511 809
Size: 507 2985 2210 1802
Fragments arranged by size:
2985 2210 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2136 2647 2677
Size: 54 1425 50 275 18 306 511 30
Cuts at: 2677 3391 3768 4365 4490 4641 4656 4951 5840
Size: 714 377 597 125 151 15 295 889
Cuts at: 5840 7469 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 714 597 511 377 306
295 275 151 125 54 50 43 30
18 15
EagI C'GGCC_G
Cuts at: 1537 1812 4951 1537
Size: 275 3139 4090
Fragments arranged by size:
4090 3139 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2033 5434 1353
Size: 680 3401 3423
Fragments arranged by size:
3423 3401 680
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3191 3970 6091 6465 6611 387
Size: 1064 138 1602 779 2121 374 146 1280
Fragments arranged by size:
2121 1602 1280 1064 779 374 146 138
EcoICRI GAG'CTC
Cuts at: 3068 4258 4690 3068
Size: 1190 432 5882
Fragments arranged by size:
5882 1190 432
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2038 2048 2114 2135 2391 2522 2639
Size: 393 406 10 66 21 256 131 117
Cuts at: 2639 3140 3166 3878 4022 5567 6164 6951 7219
Size: 501 26 712 144 1545 597 787 268
Cuts at: 7219 1239
Size: 1524
Fragments arranged by size:
1545 1524 787 712 597 501 406 393
268 256 144 131 117 66 26 21
10
EcoNI CCTnn'n_nnAGG
Cuts at: 2096 2860 2096
Size: 764 6740
EcoO109I rG'GnC_Cy
Cuts at: 2107 2191 2341 2456 2865 2938 3000 3664 4128
Size: 84 150 115 409 73 62 664 464
Cuts at: 4128 2107
Size: 5483
Fragments arranged by size:
5483 664 464 409 150 115 84 73
62
EcoRI G'AATT_C
Cuts at: 4813 5191 4813
Size: 378 7126
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2749 4477 2749
Size: 1728 5776
FspI TGC'GCA
Cuts at: 6010 6010
Size: 7504
HaeII r_GCGC'y
Cuts at: 1678 2641 3263 3691 6651 1678
Size: 963 622 428 2960 2531
Fragments arranged by size:
2960 2531 963 622 428
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2138 2170 2343 2375 2425
Size: 32 135 32 566 32 173 32 50
Cuts at: 2425 2457 3595 3627 3827 3859 4120 4152 4500
Size: 32 1138 32 200 32 261 32 348
Cuts at: 4500 4532 4730 4762 6145 6177 6219 6251 1373
Size: 32 198 32 1383 32 42 32 2626
Fragments arranged by size:
2626 1383 1138 566 348 261 200 198
173 135 50 42 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4848 5094 669
Size: 988 281 2910 246 3079
Fragments arranged by size:
3079 2910 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7504
HpaI GTT'AAC
Cuts at: 5094 5094
Size: 7504
KpnI G_GTAC'C
Cuts at: 4829 4829
Size: 7504
MfeI C'AATT_G
Cuts at: 5103 5103
Size: 7504
MluI A'CGCG_T
Cuts at: 4610 7380 4610
Size: 2770 4734
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6618 6802 1305
Size: 5313 184 2007
Fragments arranged by size:
5313 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2138 2649 4658 10
Size: 54 1425 343 306 511 2009 2856
Fragments arranged by size:
2856 2009 1425 511 343 306 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2905 2970 3138 3287 5462 5821 5980
Size: 648 1739 65 168 149 2175 359 159
Cuts at: 5980 518
Size: 2042
Fragments arranged by size:
2175 2042 1739 648 359 168 159 149
65
MspA1I CmG'CkG
Cuts at: 1606 2235 2584 2962 3396 3449 3568 3840 4590
Size: 629 349 378 434 53 119 272 750
Cuts at: 4590 4762 4920 5597 6745 6990 1606
Size: 172 158 677 1148 245 2120
Fragments arranged by size:
2120 1148 750 677 629 434 378 349
272 245 172 158 119 53
NcoI C'CATG_G
Cuts at: 513 1065 3620 3646 4176 513
Size: 552 2555 26 530 3841
Fragments arranged by size:
3841 2555 552 530 26
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7504
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7504
NotI GC'GGCC_GC
Cuts at: 4951 4951
Size: 7504
NspI r_CATG'y
Cuts at: 1132 1229 2480 2749 4862 6407 1132
Size: 97 1251 269 2113 1545 2229
Fragments arranged by size:
2229 2113 1545 1251 269 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7504
PciI A'CATG_T
Cuts at: 2745 6403 2745
Size: 3658 3846
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2212 2712 3558 1108
Size: 1104 500 846 5054
Fragments arranged by size:
5054 1104 846 500
PfoI T'CCnGG_A
Cuts at: 2294 2568 2595 3793 4629 2294
Size: 274 27 1198 836 5169
Fragments arranged by size:
5169 1198 836 274 27
PmeI GTTT'AAAC
Cuts at: 4808 4808
Size: 7504
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2242 2274 3914 3946 4386 4418 5573 5605 7086
Size: 32 1640 32 440 32 1155 32 1481
Cuts at: 7086 7118 2242
Size: 32 2628
Fragments arranged by size:
2628 1640 1481 1155 440 32 32 32
32 32
PpuMI rG'GwC_Cy
Cuts at: 2107 2191 2341 2456 2865 3000 3664 4128 2107
Size: 84 150 115 409 135 664 464 5483
Fragments arranged by size:
5483 664 464 409 150 135 115 84
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7504
PsiI TTA'TAA
Cuts at: 5074 5074
Size: 7504
PspOMI G'GGCC_C
Cuts at: 2910 2938 3321 2910
Size: 28 383 7093
Fragments arranged by size:
7093 383 28
PstI C_TGCA'G
Cuts at: 830 4856 830
Size: 4026 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7504
PvuII CAG'CTG
Cuts at: 2584 2962 3449 3568 2584
Size: 378 487 119 6520
Fragments arranged by size:
6520 487 378 119
SacI G_AGCT'C
Cuts at: 3070 4260 4692 3070
Size: 1190 432 5882
Fragments arranged by size:
5882 1190 432
SalI G'TCGA_C
Cuts at: 1936 4846 1936
Size: 2910 4594
SanDI GG'GwC_CC
Cuts at: 2191 2191
Size: 7504
SbfI CC_TGCA'GG
Cuts at: 4856 4856
Size: 7504
ScaI AGT'ACT
Cuts at: 5752 7464 5752
Size: 1712 5792
SexAI A'CCwGG_T
Cuts at: 4710 4710
Size: 7504
SfcI C'TryA_G
Cuts at: 826 1045 2957 3247 3535 4852 5987 6668 6859
Size: 219 1912 290 288 1317 1135 681 191
Cuts at: 6859 826
Size: 1471
Fragments arranged by size:
1912 1471 1317 1135 681 290 288 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7504
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7504
SmaI CCC'GGG
Cuts at: 1872 2450 3662 4831 1872
Size: 578 1212 1169 4545
Fragments arranged by size:
4545 1212 1169 578
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2753 2876 3069 3164 4441 4727
Size: 197 926 810 123 193 95 1277 286
Cuts at: 4727 5608 6509 6771 820
Size: 881 901 262 1553
Fragments arranged by size:
1553 1277 926 901 881 810 286 262
197 193 123 95
SnaBI TAC'GTA
Cuts at: 493 5295 493
Size: 4802 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7504
SphI G_CATG'C
Cuts at: 1132 4862 1132
Size: 3730 3774
SspI AAT'ATT
Cuts at: 5 52 5428 5
Size: 47 5376 2081
Fragments arranged by size:
5376 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7504
StyI C'CwwG_G
Cuts at: 513 1065 2172 2241 3003 3228 3427 3552 3620
Size: 552 1107 69 762 225 199 125 68
Cuts at: 3620 3646 3711 4176 4653 4674 4943 513
Size: 26 65 465 477 21 269 3074
Fragments arranged by size:
3074 1107 762 552 477 465 269 225
199 125 69 68 65 26 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3315 3550 4671 4709 5531 5548 5701
Size: 1255 1735 235 1121 38 822 17 153
Cuts at: 5701 5886 6331 325
Size: 185 445 1498
Fragments arranged by size:
1735 1498 1255 1121 822 445 235 185
153 38 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2742 3720 5750
Size: 274 80 33 51 1443 765 978 2030
Cuts at: 5750 7462 96
Size: 1712 138
Fragments arranged by size:
2030 1712 1443 978 765 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2175 2207 2229 2261 4651 4683 7464 7496 2175
Size: 32 22 32 2390 32 2781 32 2183
Fragments arranged by size:
2781 2390 2183 32 32 32 32 22
Tth111I GACn'n_nGTC
Cuts at: 1316 2530 1316
Size: 1214 6290
XbaI T'CTAG_A
Cuts at: 4840 4840
Size: 7504
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2657 2713 2657
Size: 56 7448
XhoI C'TCGA_G
Cuts at: 1943 2753 1943
Size: 810 6694
XmaI C'CCGG_G
Cuts at: 1870 2448 3660 4829 1870
Size: 578 1212 1169 4545
Fragments arranged by size:
4545 1212 1169 578
XmnI GAAnn'nnTTC
Cuts at: 3929 5633 3929
Size: 1704 5800
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5312 276
Size: 53 83 186 936 3778 2468
Fragments arranged by size:
3778 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SanDI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI
BstXI BstZ17I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII
SacII SapI SfiI SfoI SrfI SwaI