Restriction map for
pFN21AB8431
(Circular) (Six-base) MAPSORT of: pFN21AB8431.seq Check: 9759 from: 1 to: 6592
pFN21AB8431 6592 bp
With 182 enzymes: SgfI *
August 16, 2010 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4402 278
Size: 53 83 186 936 2866 2468
Fragments arranged by size:
2866 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3221 3935 1937
Size: 1284 714 4594
Fragments arranged by size:
4594 1284 714
Acc65I G'GTAC_C
Cuts at: 3913 3913
Size: 6592
AclI AA'CG_TT
Cuts at: 1563 4719 5092 1563
Size: 3156 373 3063
Fragments arranged by size:
3156 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2043 2115 2180 4655 6039 6307 1632
Size: 411 72 65 2475 1384 268 1917
Fragments arranged by size:
2475 1917 1384 411 268 72 65
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6395
AflIII A'CryG_T
Cuts at: 1203 3829 5491 6468 1203
Size: 2626 1662 977 1327
Fragments arranged by size:
2626 1662 1327 977
AgeI A'CCGG_T
Cuts at: 5423 5423
Size: 6592
AhdI GACnn_n'nnGTC
Cuts at: 5321 5321
Size: 6592
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6592
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3553 1184
Size: 2369 4223
AlwNI CAG_nnn'CTG
Cuts at: 4335 5907 4335
Size: 1572 5020
ApaLI G'TGCA_C
Cuts at: 4649 5805 4649
Size: 1156 5436
ApoI r'AATT_y
Cuts at: 1493 2081 2266 2390 2446 2474 2797 3093 3212
Size: 588 185 124 56 28 323 296 119
Cuts at: 3212 3355 3901 4127 4279 6388 1493
Size: 143 546 226 152 2109 1697
Fragments arranged by size:
2109 1697 588 546 323 296 226 185
152 143 124 119 56 28
AseI AT'TA_AT
Cuts at: 160 1032 2232 5146 160
Size: 872 1200 2914 1606
Fragments arranged by size:
2914 1606 1200 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6592
AvaI C'yCGr_G
Cuts at: 1870 1943 2421 3917 1870
Size: 73 478 1496 4545
Fragments arranged by size:
4545 1496 478 73
BamHI G'GATC_C
Cuts at: 3922 4289 3922
Size: 367 6225
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3913 5368 618
Size: 325 217 627 2126 1455 1842
Fragments arranged by size:
2126 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3911 1391
Size: 2520 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2134 3260 928
Size: 971 235 1126 4260
Fragments arranged by size:
4260 1126 971 235
BbvCI CC'TCA_GC
Cuts at: 3131 3131
Size: 6592
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4783 4817 1903
Size: 34 2846 34 3678
Fragments arranged by size:
3678 2846 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4484 5694 4484
Size: 1210 5382
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6592
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5203 136
Size: 107 122 71 631 605 3531 1525
Fragments arranged by size:
3531 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6587 6587
Size: 6592
BlpI GC'TnA_GC
Cuts at: 4009 4009
Size: 6592
Bme1580I G_kGCm'C
Cuts at: 1790 4653 5809 1790
Size: 2863 1156 2573
Fragments arranged by size:
2863 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6592
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4323 5281 442
Size: 416 53 83 399 24 2906 958 1753
Fragments arranged by size:
2906 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6592
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2023 3509 5252 1239
Size: 784 1486 1743 2579
Fragments arranged by size:
2579 1743 1486 784
Bpu10I CC'TnA_GC
Cuts at: 3131 3131
Size: 6592
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2513 2981 3608 4717 5582 5880 2513
Size: 468 627 1109 865 298 3225
Fragments arranged by size:
3225 1109 865 627 468 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5255 882
Size: 617 3756 2219
Fragments arranged by size:
3756 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4383 493
Size: 711 3179 2702
Fragments arranged by size:
3179 2702 711
BsaBI GATnn'nnATC
Cuts at: 3405 6586 3405
Size: 3181 3411
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4399 4781 5416 275
Size: 53 83 186 936 2866 382 635 1451
Fragments arranged by size:
2866 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5025 5423 5697 5844 6255 5025
Size: 398 274 147 411 5362
Fragments arranged by size:
5362 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6367
Fragments arranged by size:
6367 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2238 1186
Size: 1052 5540
BseYI C'CCAG_C
Cuts at: 5795 6291 5795
Size: 496 6096
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6019
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4042 4803 5831 1540
Size: 275 186 2041 761 1028 2301
Fragments arranged by size:
2301 2041 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4653 4738 5809 4653
Size: 85 1071 5436
Fragments arranged by size:
5436 1071 85
BsmI GAATG_Cn'
Cuts at: 4103 4196 4103
Size: 93 6499
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3911 4653 4738 5809 1391
Size: 399 2121 742 85 1071 2174
Fragments arranged by size:
2174 2121 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6255 6255
Size: 6592
BspHI T'CATG_A
Cuts at: 4481 6211 4481
Size: 1730 4862
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3947 844
Size: 3103 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4479 1394
Size: 429 2656 3507
Fragments arranged by size:
3507 2656 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2528 3748 5087 5261 65
Size: 1183 1280 1220 1339 174 1396
Fragments arranged by size:
1396 1339 1280 1220 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5236 5423 1700
Size: 110 3426 187 2869
Fragments arranged by size:
3426 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6592
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6592
BssSI C'ACGA_G
Cuts at: 4652 5664 4652
Size: 1012 5580
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2821 794
Size: 2027 4565
BstBI TT'CG_AA
Cuts at: 5396 5396
Size: 6592
BstEII G'GTnAC_C
Cuts at: 1184 2768 1184
Size: 1584 5008
BstXI CCAn_nnnn'nTGG
Cuts at: 2964 3637 2964
Size: 673 5919
BstYI r'GATC_y
Cuts at: 1972 3436 3922 4289 4674 4691 6143 6229 6241
Size: 1464 486 367 385 17 1452 86 12
Cuts at: 6241 6587 1972
Size: 346 1977
Fragments arranged by size:
1977 1464 1452 486 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2754 2904 513
Size: 552 939 750 150 4201
Fragments arranged by size:
4201 939 750 552 150
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2040 487
Size: 683 56 814 5039
Fragments arranged by size:
5039 814 683 56
BtsI GCAGTG_nn'
Cuts at: 4108 4902 4922 4108
Size: 794 20 5778
Fragments arranged by size:
5778 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6592
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6557
DraI TTT'AAA
Cuts at: 3896 3896
Size: 6592
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5599 809
Size: 507 4283 1802
Fragments arranged by size:
4283 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4039 4928 6557
Size: 54 1425 50 275 18 2209 889 1629
Cuts at: 6557 8
Size: 43
Fragments arranged by size:
2209 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4039 1537
Size: 275 2227 4090
Fragments arranged by size:
4090 2227 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2091 2199 4522 1353
Size: 738 108 2323 3423
Fragments arranged by size:
3423 2323 738 108
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5179 5553 5699 387
Size: 1064 138 3590 374 146 1280
Fragments arranged by size:
3590 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2023 2043 2115 2180 3509 4655 5252
Size: 393 391 20 72 65 1329 1146 597
Cuts at: 5252 6039 6307 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1329 1146 787 597 393 391 268
72 65 20
EcoNI CCTnn'n_nnAGG
Cuts at: 3206 3206
Size: 6592
EcoO109I rG'GnC_Cy
Cuts at: 2601 2614 3086 3803 2601
Size: 13 472 717 5390
Fragments arranged by size:
5390 717 472 13
EcoRI G'AATT_C
Cuts at: 3901 4279 3901
Size: 378 6214
EcoRV GAT'ATC
Cuts at: 3523 3523
Size: 6592
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2198 3185 2198
Size: 987 5605
FspI TGC'GCA
Cuts at: 5098 5098
Size: 6592
HaeII r_GCGC'y
Cuts at: 1678 5739 1678
Size: 4061 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5233 5265 5307 5339 1373
Size: 32 135 32 3661 32 42 32 2626
Fragments arranged by size:
3661 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2557 2787 3495 3827 3936 4182
Size: 988 281 619 230 708 332 109 246
Cuts at: 4182 669
Size: 3079
Fragments arranged by size:
3079 988 708 619 332 281 246 230
109
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6592
HpaI GTT'AAC
Cuts at: 2787 4182 2787
Size: 1395 5197
KpnI G_GTAC'C
Cuts at: 3917 3917
Size: 6592
MfeI C'AATT_G
Cuts at: 4191 4191
Size: 6592
MluI A'CGCG_T
Cuts at: 6468 6468
Size: 6592
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5706 5890 1305
Size: 4401 184 2007
Fragments arranged by size:
4401 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4770
Fragments arranged by size:
4770 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4550 4909 5068 518
Size: 648 3384 359 159 2042
Fragments arranged by size:
3384 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4008 4685 5833 6078 1606
Size: 2402 677 1148 245 2120
Fragments arranged by size:
2402 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2754 2904 513
Size: 552 939 750 150 4201
Fragments arranged by size:
4201 939 750 552 150
NdeI CA'TA_TG
Cuts at: 387 3564 387
Size: 3177 3415
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6592
NotI GC'GGCC_GC
Cuts at: 4039 4039
Size: 6592
NspI r_CATG'y
Cuts at: 1132 1229 2204 2824 3507 3576 3833 3950 5495
Size: 97 975 620 683 69 257 117 1545
Cuts at: 5495 1132
Size: 2229
Fragments arranged by size:
2229 1545 975 683 620 257 117 97
69
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6592
PciI A'CATG_T
Cuts at: 3829 5491 3829
Size: 1662 4930
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6592
PmeI GTTT'AAAC
Cuts at: 3896 3896
Size: 6592
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4661 4693 6174 6206 4661
Size: 32 1481 32 5047
Fragments arranged by size:
5047 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2601 2614 3086 3803 2601
Size: 13 472 717 5390
Fragments arranged by size:
5390 717 472 13
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6592
PsiI TTA'TAA
Cuts at: 2388 4162 2388
Size: 1774 4818
PstI C_TGCA'G
Cuts at: 830 3350 3944 830
Size: 2520 594 3478
Fragments arranged by size:
3478 2520 594
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6592
SalI G'TCGA_C
Cuts at: 1936 3934 1936
Size: 1998 4594
SanDI GG'GwC_CC
Cuts at: 2601 2614 3803 2601
Size: 13 1189 5390
Fragments arranged by size:
5390 1189 13
SapI GCTCTTCn'nnn_
Cuts at: 2199 2199
Size: 6592
SbfI CC_TGCA'GG
Cuts at: 3944 3944
Size: 6592
ScaI AGT'ACT
Cuts at: 4840 6552 4840
Size: 1712 4880
SfcI C'TryA_G
Cuts at: 826 1045 3023 3346 3940 5075 5756 5947 826
Size: 219 1978 323 594 1135 681 191 1471
Fragments arranged by size:
1978 1471 1135 681 594 323 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6592
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6592
SmaI CCC'GGG
Cuts at: 1872 3919 1872
Size: 2047 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2421 2528 2996 3587 4696 5597
Size: 197 926 478 107 468 591 1109 901
Cuts at: 5597 5859 820
Size: 262 1553
Fragments arranged by size:
1553 1109 926 901 591 478 468 262
197 107
SnaBI TAC'GTA
Cuts at: 493 4383 493
Size: 3890 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6592
SphI G_CATG'C
Cuts at: 1132 2204 3950 1132
Size: 1072 1746 3774
Fragments arranged by size:
3774 1746 1072
SspI AAT'ATT
Cuts at: 5 52 2106 4516 5
Size: 47 2054 2410 2081
Fragments arranged by size:
2410 2081 2054 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6592
StyI C'CwwG_G
Cuts at: 513 1065 2004 2754 2904 2963 4031 513
Size: 552 939 750 150 59 1068 3074
Fragments arranged by size:
3074 1068 939 750 552 150 59
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4619 4636 4789 4974 5419 325
Size: 1255 3039 17 153 185 445 1498
Fragments arranged by size:
3039 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2877 4838 6550
Size: 274 80 33 51 1443 900 1961 1712
Cuts at: 6550 96
Size: 138
Fragments arranged by size:
1961 1712 1443 900 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6552 6584 6552
Size: 32 6560
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6592
XbaI T'CTAG_A
Cuts at: 3928 3928
Size: 6592
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2446 3814 2446
Size: 1368 5224
XhoI C'TCGA_G
Cuts at: 1943 2421 1943
Size: 478 6114
XmaI C'CCGG_G
Cuts at: 1870 3917 1870
Size: 2047 4545
XmnI GAAnn'nnTTC
Cuts at: 4721 4721
Size: 6592
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4400 276
Size: 53 83 186 936 2866 2468
Fragments arranged by size:
2866 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI SalI SanDI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SexAI SfiI SfoI SrfI SwaI