Restriction map for
pFN21AB8427
(Circular) (Six-base) MAPSORT of: pFN21AB8427.seq Check: 5261 from: 1 to: 7420
pFN21AB8427 7420 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5230 278
Size: 53 83 186 936 3694 2468
Fragments arranged by size:
3694 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2259 4389 4763 1937
Size: 322 2130 374 4594
Fragments arranged by size:
4594 2130 374 322
Acc65I G'GTAC_C
Cuts at: 4741 4741
Size: 7420
AclI AA'CG_TT
Cuts at: 1563 5547 5920 1563
Size: 3984 373 3063
Fragments arranged by size:
3984 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2267 2487 3179 3500 3800 4103 4188 4193
Size: 635 220 692 321 300 303 85 5
Cuts at: 4193 4607 5483 6867 7135 1632
Size: 414 876 1384 268 1917
Fragments arranged by size:
1917 1384 876 692 635 414 321 303
300 268 220 85 5
AflII C'TTAA_G
Cuts at: 820 1017 2224 2789 820
Size: 197 1207 565 5451
Fragments arranged by size:
5451 1207 565 197
AflIII A'CryG_T
Cuts at: 1203 4256 6319 7296 1203
Size: 3053 2063 977 1327
Fragments arranged by size:
3053 2063 1327 977
AgeI A'CCGG_T
Cuts at: 6251 6251
Size: 7420
AhdI GACnn_n'nnGTC
Cuts at: 2046 6149 2046
Size: 4103 3317
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7420
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7420
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4631 4663 4631
Size: 32 7388
AlwNI CAG_nnn'CTG
Cuts at: 3410 4703 5163 6735 3410
Size: 1293 460 1572 4095
Fragments arranged by size:
4095 1572 1293 460
ApaLI G'TGCA_C
Cuts at: 5477 6633 5477
Size: 1156 6264
ApoI r'AATT_y
Cuts at: 1493 2400 4411 4729 4955 5107 7216 1493
Size: 907 2011 318 226 152 2109 1697
Fragments arranged by size:
2109 2011 1697 907 318 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2432 3803 4343 5974 160
Size: 872 1400 1371 540 1631 1606
Fragments arranged by size:
1631 1606 1400 1371 872 540
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7420
AvaI C'yCGr_G
Cuts at: 1870 1943 4745 1870
Size: 73 2802 4545
Fragments arranged by size:
4545 2802 73
BamHI G'GATC_C
Cuts at: 2878 4750 5117 2878
Size: 1872 367 5181
Fragments arranged by size:
5181 1872 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2414 4741 6196 618
Size: 325 217 627 627 2327 1455 1842
Fragments arranged by size:
2327 1842 1455 627 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4739 1391
Size: 3348 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2202 2273 2837 2855 928
Size: 971 303 71 564 18 5493
Fragments arranged by size:
5493 971 564 303 71 18
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5611 5645 1903
Size: 34 3674 34 3678
Fragments arranged by size:
3678 3674 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5312 6522 5312
Size: 1210 6210
BclI T'GATC_A
Cuts at: 1548 3187 1548
Size: 1639 5781
BfrBI ATG'CAT
Cuts at: 3307 3307
Size: 7420
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6031 136
Size: 107 122 71 631 605 4359 1525
Fragments arranged by size:
4359 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3928 7415 3928
Size: 3487 3933
BlpI GC'TnA_GC
Cuts at: 3981 4837 3981
Size: 856 6564
Bme1580I G_kGCm'C
Cuts at: 1790 5481 6637 1790
Size: 3691 1156 2573
Fragments arranged by size:
3691 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7420
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5151 6109 442
Size: 416 53 83 399 24 3734 958 1753
Fragments arranged by size:
3734 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 4409 1056
Size: 3353 4067
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 6080 1239
Size: 4841 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2191 3987 4295 5545 6410 6708 2191
Size: 1796 308 1250 865 298 2903
Fragments arranged by size:
2903 1796 1250 865 308 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6083 882
Size: 617 4584 2219
Fragments arranged by size:
4584 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5211 493
Size: 711 4007 2702
Fragments arranged by size:
4007 2702 711
BsaBI GATnn'nnATC
Cuts at: 7414 7414
Size: 7420
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5227 5609 6244 275
Size: 53 83 186 936 3694 382 635 1451
Fragments arranged by size:
3694 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5853 6251 6525 6672 7083 5853
Size: 398 274 147 411 6190
Fragments arranged by size:
6190 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4631 4661 1178
Size: 30 165 30 3228 30 3937
Fragments arranged by size:
3937 3228 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3750 1186
Size: 2564 4856
BseYI C'CCAG_C
Cuts at: 6623 7119 6623
Size: 496 6924
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6847
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4870 5631 6659 1540
Size: 275 186 2869 761 1028 2301
Fragments arranged by size:
2869 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2832 5481 5566 6637 2832
Size: 2649 85 1071 3615
Fragments arranged by size:
3615 2649 1071 85
BsmI GAATG_Cn'
Cuts at: 2171 2364 4931 5024 2171
Size: 193 2567 93 4567
Fragments arranged by size:
4567 2567 193 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2832 4739 5481 5566 6637 1391
Size: 399 1042 1907 742 85 1071 2174
Fragments arranged by size:
2174 1907 1071 1042 742 399 85
BspEI T'CCGG_A
Cuts at: 7083 7083
Size: 7420
BspHI T'CATG_A
Cuts at: 5309 7039 5309
Size: 1730 5690
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4775 844
Size: 3931 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5307 1394
Size: 429 3484 3507
Fragments arranged by size:
3507 3484 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4668 5915 6089 65
Size: 1183 3420 1247 174 1396
Fragments arranged by size:
3420 1396 1247 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6064 6251 1700
Size: 110 4254 187 2869
Fragments arranged by size:
4254 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7420
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7420
BssSI C'ACGA_G
Cuts at: 5480 6492 5480
Size: 1012 6408
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3665 794
Size: 2871 4549
BstBI TT'CG_AA
Cuts at: 6224 6224
Size: 7420
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7420
BstYI r'GATC_y
Cuts at: 1972 2782 2878 3928 4750 5117 5502 5519 6971
Size: 810 96 1050 822 367 385 17 1452
Cuts at: 6971 7057 7069 7415 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1050 822 810 385 367 346
96 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5929
Fragments arranged by size:
5929 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3971 487
Size: 683 56 2745 3936
Fragments arranged by size:
3936 2745 683 56
BtsI GCAGTG_nn'
Cuts at: 2013 2366 3614 4936 5730 5750 2013
Size: 353 1248 1322 794 20 3683
Fragments arranged by size:
3683 1322 1248 794 353 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7420
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7385
DraI TTT'AAA
Cuts at: 4724 4724
Size: 7420
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6427 809
Size: 507 5111 1802
Fragments arranged by size:
5111 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4867 5756 7385
Size: 54 1425 50 275 18 3037 889 1629
Cuts at: 7385 8
Size: 43
Fragments arranged by size:
3037 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4867 1537
Size: 275 3055 4090
Fragments arranged by size:
4090 3055 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2655 2896 4059 4593 5350 1353
Size: 1302 241 1163 534 757 3423
Fragments arranged by size:
3423 1302 1163 757 534 241
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6007 6381 6527 387
Size: 1064 138 4418 374 146 1280
Fragments arranged by size:
4418 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2267 2487 3179 3500 3800 4103 4188
Size: 393 635 220 692 321 300 303 85
Cuts at: 4188 4193 4607 5483 6080 6867 7135 1239
Size: 5 414 876 597 787 268 1524
Fragments arranged by size:
1524 876 787 692 635 597 414 393
321 303 300 268 220 85 5
EcoNI CCTnn'n_nnAGG
Cuts at: 2490 3959 2490
Size: 1469 5951
EcoO109I rG'GnC_Cy
Cuts at: 3520 3520
Size: 7420
EcoRI G'AATT_C
Cuts at: 4729 5107 4729
Size: 378 7042
EcoRV GAT'ATC
Cuts at: 4495 4495
Size: 7420
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3072 3072
Size: 7420
FspI TGC'GCA
Cuts at: 5926 5926
Size: 7420
HaeII r_GCGC'y
Cuts at: 1678 2991 6567 1678
Size: 1313 3576 2531
Fragments arranged by size:
3576 2531 1313
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2032 2064 2109 2141 4592
Size: 32 135 32 460 32 45 32 2451
Cuts at: 4592 4624 6061 6093 6135 6167 1373
Size: 32 1437 32 42 32 2626
Fragments arranged by size:
2626 2451 1437 460 135 45 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2385 2770 3276 4764 5010 669
Size: 988 281 447 385 506 1488 246 3079
Fragments arranged by size:
3079 1488 988 506 447 385 281 246
HindIII A'AGCT_T
Cuts at: 748 3499 748
Size: 2751 4669
HpaI GTT'AAC
Cuts at: 2385 3276 5010 2385
Size: 891 1734 4795
Fragments arranged by size:
4795 1734 891
KpnI G_GTAC'C
Cuts at: 4745 4745
Size: 7420
MfeI C'AATT_G
Cuts at: 5019 5019
Size: 7420
MluI A'CGCG_T
Cuts at: 7296 7296
Size: 7420
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2309 3761 6534 6718 1305
Size: 1004 1452 2773 184 2007
Fragments arranged by size:
2773 2007 1452 1004 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5598
Fragments arranged by size:
5598 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2957 5378 5737 5896 518
Size: 648 1791 2421 359 159 2042
Fragments arranged by size:
2421 2042 1791 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2938 4836 5513 6661 6906 1606
Size: 1332 1898 677 1148 245 2120
Fragments arranged by size:
2120 1898 1332 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5929
Fragments arranged by size:
5929 939 552
NdeI CA'TA_TG
Cuts at: 387 2946 3367 387
Size: 2559 421 4440
Fragments arranged by size:
4440 2559 421
NheI G'CTAG_C
Cuts at: 1052 4405 1052
Size: 3353 4067
NotI GC'GGCC_GC
Cuts at: 4867 4867
Size: 7420
NsiI A_TGCA'T
Cuts at: 3309 3309
Size: 7420
NspI r_CATG'y
Cuts at: 1132 1229 4260 4778 6323 1132
Size: 97 3031 518 1545 2229
Fragments arranged by size:
3031 2229 1545 518 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7420
PciI A'CATG_T
Cuts at: 4256 6319 4256
Size: 2063 5357
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3058 1108
Size: 1950 5470
PfoI T'CCnGG_A
Cuts at: 3781 3781
Size: 7420
PmeI GTTT'AAAC
Cuts at: 4724 4724
Size: 7420
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4631 4663 5489 5521 7002 7034 4631
Size: 32 826 32 1481 32 5017
Fragments arranged by size:
5017 1481 826 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3520 3520
Size: 7420
PshAI GACnn'nnGTC
Cuts at: 1613 3329 1613
Size: 1716 5704
PsiI TTA'TAA
Cuts at: 4990 4990
Size: 7420
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4164 4196 4164
Size: 32 7388
PstI C_TGCA'G
Cuts at: 830 4772 830
Size: 3942 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7420
PvuII CAG'CTG
Cuts at: 2938 2938
Size: 7420
SalI G'TCGA_C
Cuts at: 1936 4762 1936
Size: 2826 4594
SapI GCTCTTCn'nnn_
Cuts at: 4059 4059
Size: 7420
SbfI CC_TGCA'GG
Cuts at: 4772 4772
Size: 7420
ScaI AGT'ACT
Cuts at: 3639 4225 5668 7380 3639
Size: 586 1443 1712 3679
Fragments arranged by size:
3679 1712 1443 586
SfcI C'TryA_G
Cuts at: 826 1045 2375 3670 4768 5903 6584 6775 826
Size: 219 1330 1295 1098 1135 681 191 1471
Fragments arranged by size:
1471 1330 1295 1135 1098 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7420
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7420
SmaI CCC'GGG
Cuts at: 1872 4747 1872
Size: 2875 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2170 2224 2789 4002 4310 5524
Size: 197 926 227 54 565 1213 308 1214
Cuts at: 5524 6425 6687 820
Size: 901 262 1553
Fragments arranged by size:
1553 1214 1213 926 901 565 308 262
227 197 54
SnaBI TAC'GTA
Cuts at: 493 5211 493
Size: 4718 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7420
SphI G_CATG'C
Cuts at: 1132 4778 1132
Size: 3646 3774
SspI AAT'ATT
Cuts at: 5 52 2637 2718 2725 3111 3597 3793 5344
Size: 47 2585 81 7 386 486 196 1551
Cuts at: 5344 5
Size: 2081
Fragments arranged by size:
2585 2081 1551 486 386 196 81 47
7
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7420
StyI C'CwwG_G
Cuts at: 513 1065 2004 3255 4611 4859 513
Size: 552 939 1251 1356 248 3074
Fragments arranged by size:
3074 1356 1251 939 552 248
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5447 5464 5617 5802 6247 325
Size: 1255 3867 17 153 185 445 1498
Fragments arranged by size:
3867 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2052 3577 3637
Size: 274 80 33 51 1443 75 1525 60
Cuts at: 3637 4223 4372 4432 5666 7378 96
Size: 586 149 60 1234 1712 138
Fragments arranged by size:
1712 1525 1443 1234 586 274 149 138
80 75 60 60 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7380 7412 7380
Size: 32 7388
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7420
XbaI T'CTAG_A
Cuts at: 3214 4756 3214
Size: 1542 5878
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4676 4676
Size: 7420
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7420
XmaI C'CCGG_G
Cuts at: 1870 4745 1870
Size: 2875 4545
XmnI GAAnn'nnTTC
Cuts at: 2761 2910 4206 5549 2761
Size: 149 1296 1343 4632
Fragments arranged by size:
4632 1343 1296 149
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5228 276
Size: 53 83 186 936 3694 2468
Fragments arranged by size:
3694 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PsrI PstI PvuI PvuII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PspOMI PspXI RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI SwaI