Restriction map for
pFN21AB8419
(Circular) (Six-base) MAPSORT of: pFN21AB8419.seq Check: 9764 from: 1 to: 6292
pFN21AB8419 6292 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4102 278
Size: 53 83 186 936 2566 2468
Fragments arranged by size:
2566 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2388 3635 1937
Size: 451 1247 4594
Fragments arranged by size:
4594 1247 451
Acc65I G'GTAC_C
Cuts at: 3613 3613
Size: 6292
AclI AA'CG_TT
Cuts at: 1563 4419 4792 1563
Size: 2856 373 3063
Fragments arranged by size:
3063 2856 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2142 2410 2578 2746 3334 3418 4355 5739
Size: 510 268 168 168 588 84 937 1384
Cuts at: 5739 6007 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 937 588 510 268 268 168
168 84
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6095
AflIII A'CryG_T
Cuts at: 1203 5191 6168 1203
Size: 3988 977 1327
Fragments arranged by size:
3988 1327 977
AgeI A'CCGG_T
Cuts at: 5123 5123
Size: 6292
AhdI GACnn_n'nnGTC
Cuts at: 5021 5021
Size: 6292
AleI CACnn'nnGTG
Cuts at: 1166 2889 3284 1166
Size: 1723 395 4174
Fragments arranged by size:
4174 1723 395
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2220 1184
Size: 1036 5256
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3523 3555 3523
Size: 32 6260
AlwNI CAG_nnn'CTG
Cuts at: 4035 5607 4035
Size: 1572 4720
ApaLI G'TGCA_C
Cuts at: 4349 5505 4349
Size: 1156 5136
ApoI r'AATT_y
Cuts at: 1493 2293 2374 3010 3468 3580 3601 3827 3979
Size: 800 81 636 458 112 21 226 152
Cuts at: 3979 6088 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 800 636 458 226 152 112
81 21
AseI AT'TA_AT
Cuts at: 160 1032 4846 160
Size: 872 3814 1606
Fragments arranged by size:
3814 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6292
AvaI C'yCGr_G
Cuts at: 1870 1943 2660 3617 1870
Size: 73 717 957 4545
Fragments arranged by size:
4545 957 717 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3397 3430 3397
Size: 33 6259
BamHI G'GATC_C
Cuts at: 3622 3989 3622
Size: 367 5925
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3375 3613 5068 618
Size: 325 217 627 1588 238 1455 1842
Fragments arranged by size:
1842 1588 1455 627 325 238 217
BanII G_rGCy'C
Cuts at: 1391 3611 1391
Size: 2220 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5321
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4483 4517 1903
Size: 34 2546 34 3678
Fragments arranged by size:
3678 2546 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4184 5394 4184
Size: 1210 5082
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6292
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4903 136
Size: 107 122 71 631 605 3231 1525
Fragments arranged by size:
3231 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6287 6287
Size: 6292
BlpI GC'TnA_GC
Cuts at: 3709 3709
Size: 6292
Bme1580I G_kGCm'C
Cuts at: 1790 2828 4353 5509 1790
Size: 1038 1525 1156 2573
Fragments arranged by size:
2573 1525 1156 1038
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6292
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4023 4981 442
Size: 416 53 83 399 24 2606 958 1753
Fragments arranged by size:
2606 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6292
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2826 4952 1239
Size: 1587 2126 2579
Fragments arranged by size:
2579 2126 1587
Bpu10I CC'TnA_GC
Cuts at: 3188 3188
Size: 6292
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2933 4417 5282 5580 2933
Size: 1484 865 298 3645
Fragments arranged by size:
3645 1484 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2035 4955 882
Size: 617 536 2920 2219
Fragments arranged by size:
2920 2219 617 536
BsaAI yAC'GTr
Cuts at: 493 1204 3475 4083 493
Size: 711 2271 608 2702
Fragments arranged by size:
2702 2271 711 608
BsaBI GATnn'nnATC
Cuts at: 6286 6286
Size: 6292
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4099 4481 5116 275
Size: 53 83 186 936 2566 382 635 1451
Fragments arranged by size:
2566 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3378 4725 5123 5397 5544 5955 3378
Size: 1347 398 274 147 411 3715
Fragments arranged by size:
3715 1347 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6067
Fragments arranged by size:
6067 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2324 1186
Size: 1138 5154
BseYI C'CCAG_C
Cuts at: 5495 5991 5495
Size: 496 5796
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5719
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3742 4503 5531 1540
Size: 275 186 1741 761 1028 2301
Fragments arranged by size:
2301 1741 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4353 4438 5509 4353
Size: 85 1071 5136
Fragments arranged by size:
5136 1071 85
BsmI GAATG_Cn'
Cuts at: 2550 3131 3803 3896 2550
Size: 581 672 93 4946
Fragments arranged by size:
4946 672 581 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3580 3580
Size: 6292
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2828 3611 4353 4438 5509 1391
Size: 399 1038 783 742 85 1071 2174
Fragments arranged by size:
2174 1071 1038 783 742 399 85
BspEI T'CCGG_A
Cuts at: 5955 5955
Size: 6292
BspHI T'CATG_A
Cuts at: 2251 3517 4181 5911 2251
Size: 1266 664 1730 2632
Fragments arranged by size:
2632 1730 1266 664
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3647 844
Size: 2803 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4179 1394
Size: 429 2356 3507
Fragments arranged by size:
3507 2356 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3118 4787 4961 65
Size: 1183 1870 1669 174 1396
Fragments arranged by size:
1870 1669 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4936 5123 1700
Size: 110 3126 187 2869
Fragments arranged by size:
3126 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6292
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6292
BssSI C'ACGA_G
Cuts at: 4352 5364 4352
Size: 1012 5280
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6292
BstBI TT'CG_AA
Cuts at: 5096 5096
Size: 6292
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6292
BstXI CCAn_nnnn'nTGG
Cuts at: 2226 2226
Size: 6292
BstYI r'GATC_y
Cuts at: 1972 3622 3989 4374 4391 5843 5929 5941 6287
Size: 1650 367 385 17 1452 86 12 346
Cuts at: 6287 1972
Size: 1977
Fragments arranged by size:
1977 1650 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4801
Fragments arranged by size:
4801 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5553
Fragments arranged by size:
5553 683 56
BtsI GCAGTG_nn'
Cuts at: 3808 4602 4622 3808
Size: 794 20 5478
Fragments arranged by size:
5478 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6292
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6257
DraI TTT'AAA
Cuts at: 3596 3596
Size: 6292
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5299 809
Size: 507 3983 1802
Fragments arranged by size:
3983 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3739 4628 6257
Size: 54 1425 50 275 18 1909 889 1629
Cuts at: 6257 8
Size: 43
Fragments arranged by size:
1909 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3739 1537
Size: 275 1927 4090
Fragments arranged by size:
4090 1927 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2666 2834 3170 3568 4222 1353
Size: 1313 168 336 398 654 3423
Fragments arranged by size:
3423 1313 654 398 336 168
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4879 5253 5399 387
Size: 1064 138 3290 374 146 1280
Fragments arranged by size:
3290 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2142 2410 2578 2746 2826 3334 3418
Size: 393 510 268 168 168 80 508 84
Cuts at: 3418 4355 4952 5739 6007 1239
Size: 937 597 787 268 1524
Fragments arranged by size:
1524 937 787 597 510 508 393 268
268 168 168 84 80
EcoNI CCTnn'n_nnAGG
Cuts at: 2079 2079
Size: 6292
EcoO109I rG'GnC_Cy
Cuts at: 2460 3384 2460
Size: 924 5368
EcoRI G'AATT_C
Cuts at: 2374 3601 3979 2374
Size: 1227 378 4687
Fragments arranged by size:
4687 1227 378
FspI TGC'GCA
Cuts at: 4798 4798
Size: 6292
HaeII r_GCGC'y
Cuts at: 1678 5439 1678
Size: 3761 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2233 2261 2265 2293 2521
Size: 32 135 32 661 28 4 28 228
Cuts at: 2521 2553 2833 2865 4933 4965 5007 5039 1373
Size: 32 280 32 2068 32 42 32 2626
Fragments arranged by size:
2626 2068 661 280 228 135 42 32
32 32 32 32 32 28 28 4
HincII GTy'rAC
Cuts at: 669 1657 1938 3636 3882 669
Size: 988 281 1698 246 3079
Fragments arranged by size:
3079 1698 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2422 2590 2926 3346 748
Size: 1674 168 336 420 3694
Fragments arranged by size:
3694 1674 420 336 168
HpaI GTT'AAC
Cuts at: 3882 3882
Size: 6292
KpnI G_GTAC'C
Cuts at: 3617 3617
Size: 6292
MfeI C'AATT_G
Cuts at: 3891 3891
Size: 6292
MluI A'CGCG_T
Cuts at: 6168 6168
Size: 6292
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2396 5406 5590 1305
Size: 1091 3010 184 2007
Fragments arranged by size:
3010 2007 1091 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4470
Fragments arranged by size:
4470 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2202 2224 2355 2721 2737 2772 2889
Size: 648 1036 22 131 366 16 35 117
Cuts at: 2889 3157 3284 4250 4609 4768 518
Size: 268 127 966 359 159 2042
Fragments arranged by size:
2042 1036 966 648 366 359 268 159
131 127 117 35 22 16
MspA1I CmG'CkG
Cuts at: 1606 2878 3420 3708 4385 5533 5778 1606
Size: 1272 542 288 677 1148 245 2120
Fragments arranged by size:
2120 1272 1148 677 542 288 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4801
Fragments arranged by size:
4801 939 552
NdeI CA'TA_TG
Cuts at: 387 2734 387
Size: 2347 3945
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6292
NotI GC'GGCC_GC
Cuts at: 3739 3739
Size: 6292
NspI r_CATG'y
Cuts at: 1132 1229 3650 5195 1132
Size: 97 2421 1545 2229
Fragments arranged by size:
2421 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6292
PciI A'CATG_T
Cuts at: 5191 5191
Size: 6292
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3002 1108
Size: 1894 4398
PmeI GTTT'AAAC
Cuts at: 3596 3596
Size: 6292
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4361 4393 5874 5906 4361
Size: 32 1481 32 4747
Fragments arranged by size:
4747 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2460 3384 2460
Size: 924 5368
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6292
PsiI TTA'TAA
Cuts at: 2193 2484 2820 3324 3862 2193
Size: 291 336 504 538 4623
Fragments arranged by size:
4623 538 504 336 291
PstI C_TGCA'G
Cuts at: 830 3644 830
Size: 2814 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6292
PvuII CAG'CTG
Cuts at: 3420 3420
Size: 6292
SalI G'TCGA_C
Cuts at: 1936 3634 1936
Size: 1698 4594
SbfI CC_TGCA'GG
Cuts at: 3644 3644
Size: 6292
ScaI AGT'ACT
Cuts at: 4540 6252 4540
Size: 1712 4580
SfcI C'TryA_G
Cuts at: 826 1045 3640 4775 5456 5647 826
Size: 219 2595 1135 681 191 1471
Fragments arranged by size:
2595 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6292
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6292
SmaI CCC'GGG
Cuts at: 1872 3619 1872
Size: 1747 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2912 4396 5297 5559 820
Size: 197 926 969 1484 901 262 1553
Fragments arranged by size:
1553 1484 969 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4083 493
Size: 3590 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6292
SphI G_CATG'C
Cuts at: 1132 3650 1132
Size: 2518 3774
SspI AAT'ATT
Cuts at: 5 52 4216 5
Size: 47 4164 2081
Fragments arranged by size:
4164 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6292
StyI C'CwwG_G
Cuts at: 513 1065 2004 3731 513
Size: 552 939 1727 3074
Fragments arranged by size:
3074 1727 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4319 4336 4489 4674 5119 325
Size: 1255 2739 17 153 185 445 1498
Fragments arranged by size:
2739 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4538 6250 96
Size: 274 80 33 51 1443 2561 1712 138
Fragments arranged by size:
2561 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6252 6284 6252
Size: 32 6260
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6292
XbaI T'CTAG_A
Cuts at: 3628 3628
Size: 6292
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6292
XmaI C'CCGG_G
Cuts at: 1870 3617 1870
Size: 1747 4545
XmnI GAAnn'nnTTC
Cuts at: 2112 2423 2591 2675 3263 3431 4421 2112
Size: 311 168 84 588 168 990 3983
Fragments arranged by size:
3983 990 588 311 168 168 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4100 276
Size: 53 83 186 936 2566 2468
Fragments arranged by size:
2566 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BbeI BbvCI BfrBI
BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI