Restriction map for
pFN21AB8412
(Circular) (Six-base) MAPSORT of: pFN21AB8412.seq Check: 884 from: 1 to: 7474
pFN21AB8412 7474 bp
With 182 enzymes: SgfI *
December 8, 2014 13:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3127 3127
Size: 7474
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5284 278
Size: 53 83 186 936 3748 2468
Fragments arranged by size:
3748 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4817 1937
Size: 2880 4594
Acc65I G'GTAC_C
Cuts at: 4795 4795
Size: 7474
AclI AA'CG_TT
Cuts at: 1563 4773 5601 5974 1563
Size: 3210 828 373 3063
Fragments arranged by size:
3210 3063 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2135 2390 3053 3245 3869 4052 4265 4538
Size: 503 255 663 192 624 183 213 273
Cuts at: 4538 4575 5537 6921 7189 1632
Size: 37 962 1384 268 1917
Fragments arranged by size:
1917 1384 962 663 624 503 273 268
255 213 192 183 37
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7277
AflIII A'CryG_T
Cuts at: 1203 6373 7350 1203
Size: 5170 977 1327
Fragments arranged by size:
5170 1327 977
AgeI A'CCGG_T
Cuts at: 6305 6305
Size: 7474
AhdI GACnn_n'nnGTC
Cuts at: 6203 6203
Size: 7474
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7474
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3360 1184
Size: 2176 5298
AlwNI CAG_nnn'CTG
Cuts at: 2882 3142 3235 4385 4553 4594 4666 5217 6789
Size: 260 93 1150 168 41 72 551 1572
Cuts at: 6789 2882
Size: 3567
Fragments arranged by size:
3567 1572 1150 551 260 168 93 72
41
ApaI G_GGCC'C
Cuts at: 2090 2561 3824 2090
Size: 471 1263 5740
Fragments arranged by size:
5740 1263 471
ApaLI G'TGCA_C
Cuts at: 5531 6687 5531
Size: 1156 6318
ApoI r'AATT_y
Cuts at: 1493 4783 5009 5161 7270 1493
Size: 3290 226 152 2109 1697
Fragments arranged by size:
3290 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6028 160
Size: 872 4996 1606
Fragments arranged by size:
4996 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7474
AvaI C'yCGr_G
Cuts at: 1870 1943 2553 2950 3909 4319 4799 1870
Size: 73 610 397 959 410 480 4545
Fragments arranged by size:
4545 959 610 480 410 397 73
BamHI G'GATC_C
Cuts at: 2424 2844 4090 4185 4203 4804 5171 2424
Size: 420 1246 95 18 601 367 4727
Fragments arranged by size:
4727 1246 601 420 367 95 18
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2145 3403 4762 4795 6250
Size: 325 217 627 358 1258 1359 33 1455
Cuts at: 6250 618
Size: 1842
Fragments arranged by size:
1842 1455 1359 1258 627 358 325 217
33
BanII G_rGCy'C
Cuts at: 1391 2090 2437 2561 2637 2895 3798 3824 4680
Size: 699 347 124 76 258 903 26 856
Cuts at: 4680 4793 1391
Size: 113 4072
Fragments arranged by size:
4072 903 856 699 347 258 124 113
76 26
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2167 928
Size: 971 268 6235
Fragments arranged by size:
6235 971 268
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2917 2951 5665 5699 1903
Size: 34 980 34 2714 34 3678
Fragments arranged by size:
3678 2714 980 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5366 6576 5366
Size: 1210 6264
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7474
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4299 4634 6085
Size: 107 122 71 631 605 2627 335 1451
Cuts at: 6085 136
Size: 1525
Fragments arranged by size:
2627 1525 1451 631 605 335 122 107
71
BglII A'GATC_T
Cuts at: 7469 7469
Size: 7474
BlpI GC'TnA_GC
Cuts at: 2436 2889 3003 4891 2436
Size: 453 114 1888 5019
Fragments arranged by size:
5019 1888 453 114
Bme1580I G_kGCm'C
Cuts at: 1790 2090 2325 2561 3824 5535 6691 1790
Size: 300 235 236 1263 1711 1156 2573
Fragments arranged by size:
2573 1711 1263 1156 300 236 235
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7474
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2005 5205 6163
Size: 416 53 83 399 24 588 3200 958
Cuts at: 6163 442
Size: 1753
Fragments arranged by size:
3200 1753 958 588 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7474
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2717 2859 3441 3632 3659 4028 4532 4544
Size: 1478 142 582 191 27 369 504 12
Cuts at: 4544 4643 4727 6134 1239
Size: 99 84 1407 2579
Fragments arranged by size:
2579 1478 1407 582 504 369 191 142
99 84 27 12
Bpu10I CC'TnA_GC
Cuts at: 2528 2810 2528
Size: 282 7192
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5599 6464 6762 5599
Size: 865 298 6311
Fragments arranged by size:
6311 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2857 3102 4616 6137 882
Size: 617 1358 245 1514 1521 2219
Fragments arranged by size:
2219 1521 1514 1358 617 245
BsaAI yAC'GTr
Cuts at: 493 1204 5265 493
Size: 711 4061 2702
Fragments arranged by size:
4061 2702 711
BsaBI GATnn'nnATC
Cuts at: 7468 7468
Size: 7474
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3017 4687 5281 5663
Size: 53 83 186 936 1484 1670 594 382
Cuts at: 5663 6298 275
Size: 635 1451
Fragments arranged by size:
1670 1484 1451 936 635 594 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2463 4023 4272 5907 6305 6579 6726 7137 2463
Size: 1560 249 1635 398 274 147 411 2800
Fragments arranged by size:
2800 1635 1560 411 398 274 249 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2585 2615 2951 2981 3314
Size: 30 165 30 1182 30 336 30 333
Cuts at: 3314 3344 4010 4040 1178
Size: 30 666 30 4612
Fragments arranged by size:
4612 1182 666 336 333 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2082 2100 2376 3083 3329 3746 3794 3998
Size: 896 18 276 707 246 417 48 204
Cuts at: 3998 4001 4337 4541 4634 4769 1186
Size: 3 336 204 93 135 3891
Fragments arranged by size:
3891 896 707 417 336 276 246 204
204 135 93 48 18 3
BseYI C'CCAG_C
Cuts at: 2268 2272 2377 3052 4740 6677 7173 2268
Size: 4 105 675 1688 1937 496 2569
Fragments arranged by size:
2569 1937 1688 675 496 105 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2507 4236 1163
Size: 573 771 1729 4401
Fragments arranged by size:
4401 1729 771 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4480 4924 5685 6713 1540
Size: 275 186 2479 444 761 1028 2301
Fragments arranged by size:
2479 2301 1028 761 444 275 186
BsiHKAI G_wGCw'C
Cuts at: 2437 4107 4332 4680 5535 5620 6691 2437
Size: 1670 225 348 855 85 1071 3220
Fragments arranged by size:
3220 1670 1071 855 348 225 85
BsmI GAATG_Cn'
Cuts at: 2143 2335 4985 5078 2143
Size: 192 2650 93 4539
Fragments arranged by size:
4539 2650 192 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3008 3008
Size: 7474
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2090 2325 2437 2561 2637 2895 3798
Size: 399 300 235 112 124 76 258 903
Cuts at: 3798 3824 4107 4332 4680 4793 5535 5620 6691
Size: 26 283 225 348 113 742 85 1071
Cuts at: 6691 1391
Size: 2174
Fragments arranged by size:
2174 1071 903 742 399 348 300 283
258 235 225 124 113 112 85 76
26
BspEI T'CCGG_A
Cuts at: 7137 7137
Size: 7474
BspHI T'CATG_A
Cuts at: 5363 7093 5363
Size: 1730 5744
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2673 3127 3243 3288 3532 4356 4829 844
Size: 1829 454 116 45 244 824 473 3489
Fragments arranged by size:
3489 1829 824 473 454 244 116 45
BsrBI CCG'CTC
Cuts at: 1394 1823 3195 5361 1394
Size: 429 1372 2166 3507
Fragments arranged by size:
3507 2166 1372 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5969 6143 65
Size: 1183 4721 174 1396
Fragments arranged by size:
4721 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4167 4479 4759 6118 6305 1700
Size: 110 2357 312 280 1359 187 2869
Fragments arranged by size:
2869 2357 1359 312 280 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7474
BssHII G'CGCG_C
Cuts at: 1925 4456 1925
Size: 2531 4943
BssSI C'ACGA_G
Cuts at: 5534 6546 5534
Size: 1012 6462
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2665 3410 4594 794
Size: 1871 745 1184 3674
Fragments arranged by size:
3674 1871 1184 745
BstBI TT'CG_AA
Cuts at: 2927 6278 2927
Size: 3351 4123
BstEII G'GTnAC_C
Cuts at: 1184 4249 1184
Size: 3065 4409
BstXI CCAn_nnnn'nTGG
Cuts at: 4087 4234 4087
Size: 147 7327
BstYI r'GATC_y
Cuts at: 1972 2424 2844 3600 4090 4185 4203 4500 4710
Size: 452 420 756 490 95 18 297 210
Cuts at: 4710 4804 5171 5556 5573 7025 7111 7123 7469
Size: 94 367 385 17 1452 86 12 346
Cuts at: 7469 1972
Size: 1977
Fragments arranged by size:
1977 1452 756 490 452 420 385 367
346 297 210 95 94 86 18 17
12
BtgI C'CryG_G
Cuts at: 513 1065 3565 513
Size: 552 2500 4422
Fragments arranged by size:
4422 2500 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3517 487
Size: 683 56 2291 4444
Fragments arranged by size:
4444 2291 683 56
BtsI GCAGTG_nn'
Cuts at: 2325 2519 2898 3081 4990 5784 5804 2325
Size: 194 379 183 1909 794 20 3995
Fragments arranged by size:
3995 1909 794 379 194 183 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7474
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4135 4170 529
Size: 35 3571 35 3833
Fragments arranged by size:
3833 3571 35 35
DraI TTT'AAA
Cuts at: 4778 4778
Size: 7474
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6481 809
Size: 507 5165 1802
Fragments arranged by size:
5165 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2118 3624 3738
Size: 54 1425 50 275 18 288 1506 114
Cuts at: 3738 4477 4921 5810 7439 8
Size: 739 444 889 1629 43
Fragments arranged by size:
1629 1506 1425 889 739 444 288 275
114 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4477 4921 1537
Size: 275 2665 444 4090
Fragments arranged by size:
4090 2665 444 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2085 2220 2394 2507 2860 2950 3060 3886
Size: 732 135 174 113 353 90 110 826
Cuts at: 3886 4066 4337 4643 5404 1353
Size: 180 271 306 761 3423
Fragments arranged by size:
3423 826 761 732 353 306 271 180
174 135 113 110 90
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4625 4652 6061 6435 6581 387
Size: 1064 138 3036 27 1409 374 146 1280
Fragments arranged by size:
3036 1409 1280 1064 374 146 138 27
EcoICRI GAG'CTC
Cuts at: 2435 4678 2435
Size: 2243 5231
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2135 2390 2717 2859 3053 3245 3441
Size: 393 503 255 327 142 194 192 196
Cuts at: 3441 3632 3659 3869 4028 4052 4265 4532 4538
Size: 191 27 210 159 24 213 267 6
Cuts at: 4538 4544 4575 4643 4727 5537 6134 6921 7189
Size: 6 31 68 84 810 597 787 268
Cuts at: 7189 1239
Size: 1524
Fragments arranged by size:
1524 810 787 597 503 393 327 268
267 255 213 210 196 194 192 191
159 142 84 68 31 27 24 6
6
EcoNI CCTnn'n_nnAGG
Cuts at: 4319 4319
Size: 7474
EcoO109I rG'GnC_Cy
Cuts at: 2087 2557 2558 3245 3325 3502 3820 3841 3928
Size: 470 1 687 80 177 318 21 87
Cuts at: 3928 4300 2087
Size: 372 5261
Fragments arranged by size:
5261 687 470 372 318 177 87 80
21 1
EcoRI G'AATT_C
Cuts at: 4783 5161 4783
Size: 378 7096
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3621 3621
Size: 7474
FspI TGC'GCA
Cuts at: 3168 5980 3168
Size: 2812 4662
HaeII r_GCGC'y
Cuts at: 1678 2023 2740 3217 6621 1678
Size: 345 717 477 3404 2531
Fragments arranged by size:
3404 2531 717 477 345
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2585 2617 2759 2791 4010
Size: 32 135 32 1013 32 142 32 1219
Cuts at: 4010 4042 6115 6147 6189 6221 1373
Size: 32 2073 32 42 32 2626
Fragments arranged by size:
2626 2073 1219 1013 142 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4818 5064 669
Size: 988 281 2880 246 3079
Fragments arranged by size:
3079 2880 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7474
HpaI GTT'AAC
Cuts at: 5064 5064
Size: 7474
KpnI G_GTAC'C
Cuts at: 4799 4799
Size: 7474
MfeI C'AATT_G
Cuts at: 5073 5073
Size: 7474
MluI A'CGCG_T
Cuts at: 7350 7350
Size: 7474
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2379 2553 2937 6588 6772 1305
Size: 1074 174 384 3651 184 2007
Fragments arranged by size:
3651 2007 1074 384 184 174
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2120 10
Size: 54 1425 343 288 5364
Fragments arranged by size:
5364 1425 343 288 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5432 5791 5950 518
Size: 648 4266 359 159 2042
Fragments arranged by size:
4266 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2026 2170 2272 2620 3162 3847 4234 4476
Size: 420 144 102 348 542 685 387 242
Cuts at: 4476 4663 4705 4717 4740 4890 5567 6715 6960
Size: 187 42 12 23 150 677 1148 245
Cuts at: 6960 1606
Size: 2120
Fragments arranged by size:
2120 1148 685 677 542 420 387 348
245 242 187 150 144 102 42 23
12
NaeI GCC'GGC
Cuts at: 4481 4761 4481
Size: 280 7194
NcoI C'CATG_G
Cuts at: 513 1065 3565 513
Size: 552 2500 4422
Fragments arranged by size:
4422 2500 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7474
NgoMIV G'CCGG_C
Cuts at: 4479 4759 4479
Size: 280 7194
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7474
NotI GC'GGCC_GC
Cuts at: 4921 4921
Size: 7474
NspI r_CATG'y
Cuts at: 1132 1229 2399 2983 4078 4832 6377 1132
Size: 97 1170 584 1095 754 1545 2229
Fragments arranged by size:
2229 1545 1170 1095 754 584 97
PasI CC'CwG_GG
Cuts at: 1348 2407 2637 1348
Size: 1059 230 6185
Fragments arranged by size:
6185 1059 230
PciI A'CATG_T
Cuts at: 6373 6373
Size: 7474
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2882 3210 4510 4720 1108
Size: 1774 328 1300 210 3862
Fragments arranged by size:
3862 1774 1300 328 210
PmeI GTTT'AAAC
Cuts at: 4778 4778
Size: 7474
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4040 4072 5543 5575 7056 7088 4040
Size: 32 1471 32 1481 32 4426
Fragments arranged by size:
4426 1481 1471 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3245 3928 3245
Size: 683 6791
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7474
PsiI TTA'TAA
Cuts at: 3675 5044 3675
Size: 1369 6105
PspOMI G'GGCC_C
Cuts at: 2086 2557 3820 2086
Size: 471 1263 5740
Fragments arranged by size:
5740 1263 471
PstI C_TGCA'G
Cuts at: 830 2905 3240 3349 4455 4674 4704 4826 830
Size: 2075 335 109 1106 219 30 122 3478
Fragments arranged by size:
3478 2075 1106 335 219 122 109 30
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7474
PvuII CAG'CTG
Cuts at: 2272 4234 4705 4740 2272
Size: 1962 471 35 5006
Fragments arranged by size:
5006 1962 471 35
RsrII CG'GwC_CG
Cuts at: 4026 4026
Size: 7474
SacI G_AGCT'C
Cuts at: 2437 4680 2437
Size: 2243 5231
SalI G'TCGA_C
Cuts at: 1936 4816 1936
Size: 2880 4594
SanDI GG'GwC_CC
Cuts at: 3245 3245
Size: 7474
SapI GCTCTTCn'nnn_
Cuts at: 4337 4643 4337
Size: 306 7168
SbfI CC_TGCA'GG
Cuts at: 4826 4826
Size: 7474
ScaI AGT'ACT
Cuts at: 5722 7434 5722
Size: 1712 5762
SfcI C'TryA_G
Cuts at: 826 1045 2901 3236 3345 3664 3828 4451 4670
Size: 219 1856 335 109 319 164 623 219
Cuts at: 4670 4700 4822 5957 6638 6829 826
Size: 30 122 1135 681 191 1471
Fragments arranged by size:
1856 1471 1135 681 623 335 319 219
219 191 164 122 109 30
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7474
SgrAI Cr'CCGG_yG
Cuts at: 1700 4167 1700
Size: 2467 5007
SmaI CCC'GGG
Cuts at: 1872 2555 2952 3911 4801 1872
Size: 683 397 959 890 4545
Fragments arranged by size:
4545 959 890 683 397
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5578 6479 6741 820
Size: 197 926 3635 901 262 1553
Fragments arranged by size:
3635 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5265 493
Size: 4772 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7474
SphI G_CATG'C
Cuts at: 1132 2983 4832 1132
Size: 1851 1849 3774
Fragments arranged by size:
3774 1851 1849
SspI AAT'ATT
Cuts at: 5 52 5398 5
Size: 47 5346 2081
Fragments arranged by size:
5346 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7474
StyI C'CwwG_G
Cuts at: 513 1065 2654 3008 3414 3565 3741 3756 3798
Size: 552 1589 354 406 151 176 15 42
Cuts at: 3798 4080 4198 4407 4913 513
Size: 282 118 209 506 3074
Fragments arranged by size:
3074 1589 552 506 406 354 282 209
176 151 118 42 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2264 4732 5501 5518 5671 5856 6301
Size: 1255 684 2468 769 17 153 185 445
Cuts at: 6301 325
Size: 1498
Fragments arranged by size:
2468 1498 1255 769 684 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4284 5720 7432
Size: 274 80 33 51 1443 2307 1436 1712
Cuts at: 7432 96
Size: 138
Fragments arranged by size:
2307 1712 1443 1436 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3314 3346 7434 7466 3314
Size: 32 4088 32 3322
Fragments arranged by size:
4088 3322 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7474
XbaI T'CTAG_A
Cuts at: 3437 4810 3437
Size: 1373 6101
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3656 4570 3656
Size: 914 6560
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7474
XmaI C'CCGG_G
Cuts at: 1870 2553 2950 3909 4799 1870
Size: 683 397 959 890 4545
Fragments arranged by size:
4545 959 890 683 397
XmnI GAAnn'nnTTC
Cuts at: 5603 5603
Size: 7474
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5282 276
Size: 53 83 186 936 3748 2468
Fragments arranged by size:
3748 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BstZ17I Bsu36I DraIII EcoRV FseI FspAI
KasI NarI NruI NsiI PacI PfoI PmlI PspXI
PsrI SacII SexAI SfiI SfoI SrfI SwaI