Restriction map for
pFN21AB8411
(Circular) (Six-base) MAPSORT of: pFN21AB8411.seq Check: 1235 from: 1 to: 7303
pFN21AB8411 7303 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5113 278
Size: 53 83 186 936 3577 2468
Fragments arranged by size:
3577 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4646 1937
Size: 2709 4594
Acc65I G'GTAC_C
Cuts at: 4624 4624
Size: 7303
AclI AA'CG_TT
Cuts at: 1563 5430 5803 1563
Size: 3867 373 3063
Fragments arranged by size:
3867 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2622 4080 4236 4278 5366 6750 7018 1632
Size: 990 1458 156 42 1088 1384 268 1917
Fragments arranged by size:
1917 1458 1384 1088 990 268 156 42
AfeI AGC'GCT
Cuts at: 3110 3110
Size: 7303
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7106
AflIII A'CryG_T
Cuts at: 1203 3698 6202 7179 1203
Size: 2495 2504 977 1327
Fragments arranged by size:
2504 2495 1327 977
AgeI A'CCGG_T
Cuts at: 6134 6134
Size: 7303
AhdI GACnn_n'nnGTC
Cuts at: 6032 6032
Size: 7303
AleI CACnn'nnGTG
Cuts at: 1166 4035 1166
Size: 2869 4434
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2847 1184
Size: 1663 5640
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2417 2449 2417
Size: 32 7271
AlwNI CAG_nnn'CTG
Cuts at: 2545 3256 4378 5046 6618 2545
Size: 711 1122 668 1572 3230
Fragments arranged by size:
3230 1572 1122 711 668
ApaI G_GGCC'C
Cuts at: 2097 2649 3184 2097
Size: 552 535 6216
Fragments arranged by size:
6216 552 535
ApaLI G'TGCA_C
Cuts at: 5360 6516 5360
Size: 1156 6147
ApoI r'AATT_y
Cuts at: 1493 2231 2264 2636 3566 4612 4838 4990 7099
Size: 738 33 372 930 1046 226 152 2109
Cuts at: 7099 1493
Size: 1697
Fragments arranged by size:
2109 1697 1046 930 738 372 226 152
33
AseI AT'TA_AT
Cuts at: 160 1032 4125 5857 160
Size: 872 3093 1732 1606
Fragments arranged by size:
3093 1732 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7303
AvaI C'yCGr_G
Cuts at: 1870 1943 4357 4628 1870
Size: 73 2414 271 4545
Fragments arranged by size:
4545 2414 271 73
BamHI G'GATC_C
Cuts at: 4633 5000 4633
Size: 367 6936
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3816 4091 4624 6079 618
Size: 325 217 627 2029 275 533 1455 1842
Fragments arranged by size:
2029 1842 1455 627 533 325 275 217
BanII G_rGCy'C
Cuts at: 1391 2097 2649 2802 2853 3184 3195 3675 4171
Size: 706 552 153 51 331 11 480 496
Cuts at: 4171 4622 1391
Size: 451 4072
Fragments arranged by size:
4072 706 552 496 480 451 331 153
51 11
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2445 2517 2739 3006 3489 928
Size: 971 546 72 222 267 483 4742
Fragments arranged by size:
4742 971 546 483 267 222 72
BbvCI CC'TCA_GC
Cuts at: 2157 2703 2157
Size: 546 6757
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5494 5528 1903
Size: 34 3557 34 3678
Fragments arranged by size:
3678 3557 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5195 6405 5195
Size: 1210 6093
BclI T'GATC_A
Cuts at: 1548 2494 1548
Size: 946 6357
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3644 5914 136
Size: 107 122 71 631 605 1972 2270 1525
Fragments arranged by size:
2270 1972 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7298 7298
Size: 7303
BlpI GC'TnA_GC
Cuts at: 3674 4720 3674
Size: 1046 6257
Bme1580I G_kGCm'C
Cuts at: 1790 2097 2649 3184 3821 4096 4500 5364 6520
Size: 307 552 535 637 275 404 864 1156
Cuts at: 6520 1790
Size: 2573
Fragments arranged by size:
2573 1156 864 637 552 535 404 307
275
BmgBI CAC'GTC
Cuts at: 1318 3410 1318
Size: 2092 5211
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2621 3015 5034
Size: 416 53 83 399 24 1204 394 2019
Cuts at: 5034 5992 442
Size: 958 1753
Fragments arranged by size:
2019 1753 1204 958 416 399 394 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7303
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3743 4337 4574 5963 1239
Size: 2504 594 237 1389 2579
Fragments arranged by size:
2579 2504 1389 594 237
Bpu10I CC'TnA_GC
Cuts at: 2157 2703 2157
Size: 546 6757
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2778 5428 6293 6591 2778
Size: 2650 865 298 3490
Fragments arranged by size:
3490 2650 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5966 882
Size: 617 4467 2219
Fragments arranged by size:
4467 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3699 4495 5094 493
Size: 711 2495 796 599 2702
Fragments arranged by size:
2702 2495 796 711 599
BsaBI GATnn'nnATC
Cuts at: 7297 7297
Size: 7303
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5110 5492 6127 275
Size: 53 83 186 936 3577 382 635 1451
Fragments arranged by size:
3577 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5736 6134 6408 6555 6966 5736
Size: 398 274 147 411 6073
Fragments arranged by size:
6073 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2137 2167 3399 3429 1178
Size: 30 165 30 734 30 1232 30 5052
Fragments arranged by size:
5052 1232 734 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2104 2145 2691 3125 3593 3791 3794 1186
Size: 918 41 546 434 468 198 3 4695
Fragments arranged by size:
4695 918 546 468 434 198 41 3
BseYI C'CCAG_C
Cuts at: 2149 2670 4483 4584 6506 7002 2149
Size: 521 1813 101 1922 496 2450
Fragments arranged by size:
2450 1922 1813 521 496 101
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3170 3679 4358 1163
Size: 573 1434 509 679 4108
Fragments arranged by size:
4108 1434 679 573 509
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4753 5514 6542 1540
Size: 275 186 2752 761 1028 2301
Fragments arranged by size:
2752 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2853 3159 3195 3415 3675 3735 4560 5364 5449
Size: 306 36 220 260 60 825 804 85
Cuts at: 5449 6520 2853
Size: 1071 3636
Fragments arranged by size:
3636 1071 825 804 306 260 220 85
60 36
BsiWI C'GTAC_G
Cuts at: 4491 4491
Size: 7303
BsmI GAATG_Cn'
Cuts at: 4814 4907 4814
Size: 93 7210
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2097 2649 2802 2853 3159 3184 3195
Size: 399 307 552 153 51 306 25 11
Cuts at: 3195 3415 3675 3735 3821 4096 4171 4500 4560
Size: 220 260 60 86 275 75 329 60
Cuts at: 4560 4622 5364 5449 6520 1391
Size: 62 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 552 399 329 307 306
275 260 220 153 86 85 75 62
60 60 51 25 11
BspEI T'CCGG_A
Cuts at: 6966 6966
Size: 7303
BspHI T'CATG_A
Cuts at: 5192 6922 5192
Size: 1730 5573
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4386 4572 4658 844
Size: 3542 186 86 3489
Fragments arranged by size:
3542 3489 186 86
BsrBI CCG'CTC
Cuts at: 1394 1823 4158 5190 1394
Size: 429 2335 1032 3507
Fragments arranged by size:
3507 2335 1032 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5798 5972 65
Size: 1183 4550 174 1396
Fragments arranged by size:
4550 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5947 6134 1700
Size: 110 4137 187 2869
Fragments arranged by size:
4137 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7303
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7303
BssSI C'ACGA_G
Cuts at: 3188 5363 6375 3188
Size: 2175 1012 4116
Fragments arranged by size:
4116 2175 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4080 4378 4564 794
Size: 3286 298 186 3533
Fragments arranged by size:
3533 3286 298 186
BstBI TT'CG_AA
Cuts at: 6107 6107
Size: 7303
BstEII G'GTnAC_C
Cuts at: 1184 2143 1184
Size: 959 6344
BstXI CCAn_nnnn'nTGG
Cuts at: 2678 3721 4037 2678
Size: 1043 316 5944
Fragments arranged by size:
5944 1043 316
BstYI r'GATC_y
Cuts at: 1972 2239 3656 4633 5000 5385 5402 6854 6940
Size: 267 1417 977 367 385 17 1452 86
Cuts at: 6940 6952 7298 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1417 977 385 367 346 267
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2586 2586
Size: 7303
BtgI C'CryG_G
Cuts at: 513 1065 2004 4589 513
Size: 552 939 2585 3227
Fragments arranged by size:
3227 2585 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4580 487
Size: 683 56 3354 3210
Fragments arranged by size:
3354 3210 683 56
BtsI GCAGTG_nn'
Cuts at: 2053 3596 3883 4819 5613 5633 2053
Size: 1543 287 936 794 20 3723
Fragments arranged by size:
3723 1543 936 794 287 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7303
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7268
DraI TTT'AAA
Cuts at: 4607 4607
Size: 7303
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3809 6310 809
Size: 507 2493 2501 1802
Fragments arranged by size:
2501 2493 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2281 3036 3141
Size: 54 1425 50 275 18 451 755 105
Cuts at: 3141 3532 4750 5639 7268 8
Size: 391 1218 889 1629 43
Fragments arranged by size:
1629 1425 1218 889 755 451 391 275
105 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4750 1537
Size: 275 2938 4090
Fragments arranged by size:
4090 2938 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2004 2022 2166 2701 2754 3281 3854 3945
Size: 651 18 144 535 53 527 573 91
Cuts at: 3945 4254 5233 1353
Size: 309 979 3423
Fragments arranged by size:
3423 979 651 573 535 527 309 144
91 53 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5890 6264 6410 387
Size: 1064 138 4301 374 146 1280
Fragments arranged by size:
4301 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2851 3193 3673 2851
Size: 342 480 6481
Fragments arranged by size:
6481 480 342
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2622 3743 4080 4236 4278 4337 4574
Size: 393 990 1121 337 156 42 59 237
Cuts at: 4574 5366 5963 6750 7018 1239
Size: 792 597 787 268 1524
Fragments arranged by size:
1524 1121 990 792 787 597 393 337
268 237 156 59 42
EcoO109I rG'GnC_Cy
Cuts at: 2176 2645 2690 3181 3396 4351 4475 2176
Size: 469 45 491 215 955 124 5004
Fragments arranged by size:
5004 955 491 469 215 124 45
EcoRI G'AATT_C
Cuts at: 2264 2636 4612 4990 2264
Size: 372 1976 378 4577
Fragments arranged by size:
4577 1976 378 372
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2405 2427 2405
Size: 22 7281
FspI TGC'GCA
Cuts at: 3595 5809 3595
Size: 2214 5089
HaeII r_GCGC'y
Cuts at: 1678 3112 6450 1678
Size: 1434 3338 2531
Fragments arranged by size:
3338 2531 1434
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2444 2476 3399 3431 3532
Size: 32 135 32 872 32 923 32 101
Cuts at: 3532 3564 4182 4214 5944 5976 6018 6050 1373
Size: 32 618 32 1730 32 42 32 2626
Fragments arranged by size:
2626 1730 923 872 618 135 101 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2344 4522 4647 4893 669
Size: 988 281 406 2178 125 246 3079
Fragments arranged by size:
3079 2178 988 406 281 246 125
HindIII A'AGCT_T
Cuts at: 748 4229 748
Size: 3481 3822
HpaI GTT'AAC
Cuts at: 4893 4893
Size: 7303
KpnI G_GTAC'C
Cuts at: 4628 4628
Size: 7303
MfeI C'AATT_G
Cuts at: 4902 4902
Size: 7303
MluI A'CGCG_T
Cuts at: 7179 7179
Size: 7303
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3648 3825 6417 6601 1305
Size: 2343 177 2592 184 2007
Fragments arranged by size:
2592 2343 2007 184 177
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2283 3038 3534 10
Size: 54 1425 343 451 755 496 3779
Fragments arranged by size:
3779 1425 755 496 451 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4035 4544 5261 5620 5779 518
Size: 648 2869 509 717 359 159 2042
Fragments arranged by size:
2869 2042 717 648 509 359 159
MspA1I CmG'CkG
Cuts at: 1606 2480 3425 3538 3678 3693 3870 4086 4315
Size: 874 945 113 140 15 177 216 229
Cuts at: 4315 4591 4719 5396 6544 6789 1606
Size: 276 128 677 1148 245 2120
Fragments arranged by size:
2120 1148 945 874 677 276 245 229
216 177 140 128 113 15
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5812
Fragments arranged by size:
5812 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7303
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7303
NotI GC'GGCC_GC
Cuts at: 4750 4750
Size: 7303
NspI r_CATG'y
Cuts at: 1132 1229 4078 4549 4661 6206 1132
Size: 97 2849 471 112 1545 2229
Fragments arranged by size:
2849 2229 1545 471 112 97
PasI CC'CwG_GG
Cuts at: 1348 2642 1348
Size: 1294 6009
PciI A'CATG_T
Cuts at: 6202 6202
Size: 7303
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7303
PfoI T'CCnGG_A
Cuts at: 2251 2251
Size: 7303
PmeI GTTT'AAAC
Cuts at: 4607 4607
Size: 7303
PmlI CAC'GTG
Cuts at: 3699 3699
Size: 7303
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3428 3460 5372 5404 6885 6917 3428
Size: 32 1912 32 1481 32 3814
Fragments arranged by size:
3814 1912 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2176 3396 2176
Size: 1220 6083
PshAI GACnn'nnGTC
Cuts at: 1613 4533 1613
Size: 2920 4383
PsiI TTA'TAA
Cuts at: 3014 4873 3014
Size: 1859 5444
PspOMI G'GGCC_C
Cuts at: 2093 2645 3180 2093
Size: 552 535 6216
Fragments arranged by size:
6216 552 535
PstI C_TGCA'G
Cuts at: 830 2836 4655 830
Size: 2006 1819 3478
Fragments arranged by size:
3478 2006 1819
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7303
PvuII CAG'CTG
Cuts at: 2480 3425 3538 3678 3693 3870 2480
Size: 945 113 140 15 177 5913
Fragments arranged by size:
5913 945 177 140 113 15
SacI G_AGCT'C
Cuts at: 2853 3195 3675 2853
Size: 342 480 6481
Fragments arranged by size:
6481 480 342
SacII CC_GC'GG
Cuts at: 4592 4592
Size: 7303
SalI G'TCGA_C
Cuts at: 1936 4645 1936
Size: 2709 4594
SapI GCTCTTCn'nnn_
Cuts at: 2754 3945 2754
Size: 1191 6112
SbfI CC_TGCA'GG
Cuts at: 4655 4655
Size: 7303
ScaI AGT'ACT
Cuts at: 3758 5551 7263 3758
Size: 1793 1712 3798
Fragments arranged by size:
3798 1793 1712
SexAI A'CCwGG_T
Cuts at: 2574 2574
Size: 7303
SfcI C'TryA_G
Cuts at: 826 1045 2832 3500 3826 3901 4651 5786 6467
Size: 219 1787 668 326 75 750 1135 681
Cuts at: 6467 6658 826
Size: 191 1471
Fragments arranged by size:
1787 1471 1135 750 681 668 326 219
191 75
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7303
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7303
SmaI CCC'GGG
Cuts at: 1872 4630 1872
Size: 2758 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2793 5407 6308 6570 820
Size: 197 926 850 2614 901 262 1553
Fragments arranged by size:
2614 1553 926 901 850 262 197
SnaBI TAC'GTA
Cuts at: 493 5094 493
Size: 4601 2702
SpeI A'CTAG_T
Cuts at: 152 2863 152
Size: 2711 4592
SphI G_CATG'C
Cuts at: 1132 4661 1132
Size: 3529 3774
SspI AAT'ATT
Cuts at: 5 52 5227 5
Size: 47 5175 2081
Fragments arranged by size:
5175 2081 47
StuI AGG'CCT
Cuts at: 1517 3224 1517
Size: 1707 5596
StyI C'CwwG_G
Cuts at: 513 1065 2004 2657 4742 513
Size: 552 939 653 2085 3074
Fragments arranged by size:
3074 2085 939 653 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2326 3420 4220 4431 5330 5347 5500
Size: 1255 746 1094 800 211 899 17 153
Cuts at: 5500 5685 6130 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1094 899 800 746 445 211
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2953 3756 5549
Size: 274 80 33 51 1443 976 803 1793
Cuts at: 5549 7261 96
Size: 1712 138
Fragments arranged by size:
1793 1712 1443 976 803 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2137 2169 3161 3193 7263 7295 2137
Size: 32 992 32 4070 32 2145
Fragments arranged by size:
4070 2145 992 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2313 2339 1316
Size: 997 26 6280
Fragments arranged by size:
6280 997 26
XbaI T'CTAG_A
Cuts at: 4639 4639
Size: 7303
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7303
XmaI C'CCGG_G
Cuts at: 1870 4628 1870
Size: 2758 4545
XmnI GAAnn'nnTTC
Cuts at: 2130 2922 3863 4292 5432 2130
Size: 792 941 429 1140 4001
Fragments arranged by size:
4001 1140 941 792 429
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5111 276
Size: 53 83 186 936 3577 2468
Fragments arranged by size:
3577 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbeI BfrBI BplI BsmBI
BstZ17I DraIII EcoNI EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PspXI PsrI RsrII
SanDI SfiI SfoI SrfI SwaI XcmI