Restriction map for
pFN21AB8407
(Circular) (Six-base) MAPSORT of: pFN21AB8407.seq Check: 8396 from: 1 to: 5587
pFN21AB8407 5587 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3397 278
Size: 53 83 186 936 1861 2468
Fragments arranged by size:
2468 1861 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2930 1937
Size: 993 4594
Acc65I G'GTAC_C
Cuts at: 2908 2908
Size: 5587
AclI AA'CG_TT
Cuts at: 1563 3714 4087 1563
Size: 2151 373 3063
Fragments arranged by size:
3063 2151 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2516 3650 5034 5302 1632
Size: 884 1134 1384 268 1917
Fragments arranged by size:
1917 1384 1134 884 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5390
AflIII A'CryG_T
Cuts at: 1203 2732 4486 5463 1203
Size: 1529 1754 977 1327
Fragments arranged by size:
1754 1529 1327 977
AgeI A'CCGG_T
Cuts at: 4418 4418
Size: 5587
AhdI GACnn_n'nnGTC
Cuts at: 4316 4316
Size: 5587
AleI CACnn'nnGTG
Cuts at: 1166 2513 1166
Size: 1347 4240
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5587
AlwNI CAG_nnn'CTG
Cuts at: 2269 2571 3330 4902 2269
Size: 302 759 1572 2954
Fragments arranged by size:
2954 1572 759 302
ApaLI G'TGCA_C
Cuts at: 3644 4800 3644
Size: 1156 4431
ApoI r'AATT_y
Cuts at: 1493 2896 3122 3274 5383 1493
Size: 1403 226 152 2109 1697
Fragments arranged by size:
2109 1697 1403 226 152
AscI GG'CGCG_CC
Cuts at: 2126 2126
Size: 5587
AseI AT'TA_AT
Cuts at: 160 1032 4141 160
Size: 872 3109 1606
Fragments arranged by size:
3109 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5587
AvaI C'yCGr_G
Cuts at: 1870 1943 2912 1870
Size: 73 969 4545
Fragments arranged by size:
4545 969 73
BamHI G'GATC_C
Cuts at: 2917 3284 2917
Size: 367 5220
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2153 2908 4363 618
Size: 325 217 627 366 755 1455 1842
Fragments arranged by size:
1842 1455 755 627 366 325 217
BanII G_rGCy'C
Cuts at: 1391 2906 1391
Size: 1515 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2477 2889 928
Size: 971 578 412 3626
Fragments arranged by size:
3626 971 578 412
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3778 3812 1903
Size: 34 1841 34 3678
Fragments arranged by size:
3678 1841 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2022 3479 4689 2022
Size: 1457 1210 2920
Fragments arranged by size:
2920 1457 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5587
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4198 136
Size: 107 122 71 631 605 2526 1525
Fragments arranged by size:
2526 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5582 5582
Size: 5587
BlpI GC'TnA_GC
Cuts at: 3004 3004
Size: 5587
Bme1580I G_kGCm'C
Cuts at: 1790 2158 3648 4804 1790
Size: 368 1490 1156 2573
Fragments arranged by size:
2573 1490 1156 368
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5587
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2218 2446 3318
Size: 416 53 83 399 24 801 228 872
Cuts at: 3318 4276 442
Size: 958 1753
Fragments arranged by size:
1753 958 872 801 416 399 228 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5587
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2102 4247 1239
Size: 863 2145 2579
Fragments arranged by size:
2579 2145 863
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3712 4577 4875 3712
Size: 865 298 4424
Fragments arranged by size:
4424 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2636 4250 882
Size: 617 1137 1614 2219
Fragments arranged by size:
2219 1614 1137 617
BsaAI yAC'GTr
Cuts at: 493 1204 3378 493
Size: 711 2174 2702
Fragments arranged by size:
2702 2174 711
BsaBI GATnn'nnATC
Cuts at: 5581 5581
Size: 5587
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3394 3776 4411 275
Size: 53 83 186 936 1861 382 635 1451
Fragments arranged by size:
1861 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2150 4020 4418 4692 4839 5250 2150
Size: 1870 398 274 147 411 2487
Fragments arranged by size:
2487 1870 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5362
Fragments arranged by size:
5362 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5587
BseYI C'CCAG_C
Cuts at: 2157 4790 5286 2157
Size: 2633 496 2458
Fragments arranged by size:
2633 2458 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2051 1163
Size: 573 315 4699
Fragments arranged by size:
4699 573 315
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2055 3037 3798 4826 1540
Size: 275 186 54 982 761 1028 2301
Fragments arranged by size:
2301 1028 982 761 275 186 54
BsiHKAI G_wGCw'C
Cuts at: 2187 3648 3733 4804 2187
Size: 1461 85 1071 2970
Fragments arranged by size:
2970 1461 1071 85
BsmI GAATG_Cn'
Cuts at: 3098 3191 3098
Size: 93 5494
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2158 2187 2906 3648 3733 4804 1391
Size: 399 368 29 719 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 719 399 368 85 29
BspEI T'CCGG_A
Cuts at: 5250 5250
Size: 5587
BspHI T'CATG_A
Cuts at: 3476 5206 3476
Size: 1730 3857
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2942 844
Size: 2098 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2051 3474 1394
Size: 429 228 1423 3507
Fragments arranged by size:
3507 1423 429 228
BsrDI GCAATG_nn'
Cuts at: 65 1248 4082 4256 65
Size: 1183 2834 174 1396
Fragments arranged by size:
2834 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2020 2161 4231 4418 1700
Size: 110 210 141 2070 187 2869
Fragments arranged by size:
2869 2070 210 187 141 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5587
BssHII G'CGCG_C
Cuts at: 1925 2126 1925
Size: 201 5386
BssSI C'ACGA_G
Cuts at: 3647 4659 3647
Size: 1012 4575
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2571 794
Size: 1777 3810
BstBI TT'CG_AA
Cuts at: 4391 4391
Size: 5587
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5587
BstXI CCAn_nnnn'nTGG
Cuts at: 2515 2515
Size: 5587
BstYI r'GATC_y
Cuts at: 1972 2917 3284 3669 3686 5138 5224 5236 5582
Size: 945 367 385 17 1452 86 12 346
Cuts at: 5582 1972
Size: 1977
Fragments arranged by size:
1977 1452 945 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2025 2037 2326 513
Size: 552 939 21 12 289 3774
Fragments arranged by size:
3774 939 552 289 21 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4848
Fragments arranged by size:
4848 683 56
BtsI GCAGTG_nn'
Cuts at: 3103 3897 3917 3103
Size: 794 20 4773
Fragments arranged by size:
4773 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5587
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2796 2831 529
Size: 35 2232 35 3285
Fragments arranged by size:
3285 2232 35 35
DraI TTT'AAA
Cuts at: 2891 2891
Size: 5587
DraIII CAC_nnn'GTG
Cuts at: 2226 2226
Size: 5587
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2690 4594 809
Size: 507 1374 1904 1802
Fragments arranged by size:
1904 1802 1374 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2022 2052 3034
Size: 54 1425 50 275 18 192 30 982
Cuts at: 3034 3923 5552 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 982 889 275 192 54 50
43 30 18
EagI C'GGCC_G
Cuts at: 1537 1812 2052 3034 1537
Size: 275 240 982 4090
Fragments arranged by size:
4090 982 275 240
EarI CTCTTCn'nnn_
Cuts at: 1353 2216 2303 2372 3517 1353
Size: 863 87 69 1145 3423
Fragments arranged by size:
3423 1145 863 87 69
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2061 2696 4174 4548 4694 387
Size: 1064 138 472 635 1478 374 146 1280
Fragments arranged by size:
1478 1280 1064 635 472 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2102 2516 3650 4247 5034 5302 1239
Size: 393 470 414 1134 597 787 268 1524
Fragments arranged by size:
1524 1134 787 597 470 414 393 268
EcoRI G'AATT_C
Cuts at: 2896 3274 2896
Size: 378 5209
FspI TGC'GCA
Cuts at: 4093 4093
Size: 5587
HaeII r_GCGC'y
Cuts at: 1678 4734 1678
Size: 3056 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4228 4260 4302 4334 1373
Size: 32 135 32 2656 32 42 32 2626
Fragments arranged by size:
2656 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2931 3177 669
Size: 988 281 993 246 3079
Fragments arranged by size:
3079 993 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5587
HpaI GTT'AAC
Cuts at: 3177 3177
Size: 5587
KpnI G_GTAC'C
Cuts at: 2912 2912
Size: 5587
MfeI C'AATT_G
Cuts at: 3186 3186
Size: 5587
MluI A'CGCG_T
Cuts at: 5463 5463
Size: 5587
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4701 4885 1305
Size: 3396 184 2007
Fragments arranged by size:
3396 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3765
Fragments arranged by size:
3765 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2513 3545 3904 4063 518
Size: 648 1347 1032 359 159 2042
Fragments arranged by size:
2042 1347 1032 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2039 2708 2781 3003 3680 4828 5073 1606
Size: 433 669 73 222 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 669 433 245 222 73
NaeI GCC'GGC
Cuts at: 2022 2022
Size: 5587
NcoI C'CATG_G
Cuts at: 513 1065 2004 2326 513
Size: 552 939 322 3774
Fragments arranged by size:
3774 939 552 322
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5587
NgoMIV G'CCGG_C
Cuts at: 2020 2020
Size: 5587
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5587
NotI GC'GGCC_GC
Cuts at: 3034 3034
Size: 5587
NspI r_CATG'y
Cuts at: 1132 1229 2297 2736 2945 4490 1132
Size: 97 1068 439 209 1545 2229
Fragments arranged by size:
2229 1545 1068 439 209 97
PasI CC'CwG_GG
Cuts at: 1348 2827 1348
Size: 1479 4108
PciI A'CATG_T
Cuts at: 2732 4486 2732
Size: 1754 3833
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2164 2269 2332 1108
Size: 1056 105 63 4363
Fragments arranged by size:
4363 1056 105 63
PmeI GTTT'AAAC
Cuts at: 2891 2891
Size: 5587
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3656 3688 5169 5201 3656
Size: 32 1481 32 4042
Fragments arranged by size:
4042 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5587
PsiI TTA'TAA
Cuts at: 3157 3157
Size: 5587
PstI C_TGCA'G
Cuts at: 830 2567 2939 830
Size: 1737 372 3478
Fragments arranged by size:
3478 1737 372
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5587
PvuII CAG'CTG
Cuts at: 2708 2708
Size: 5587
SacII CC_GC'GG
Cuts at: 2040 2040
Size: 5587
SalI G'TCGA_C
Cuts at: 1936 2929 1936
Size: 993 4594
SapI GCTCTTCn'nnn_
Cuts at: 2372 2372
Size: 5587
SbfI CC_TGCA'GG
Cuts at: 2939 2939
Size: 5587
ScaI AGT'ACT
Cuts at: 3835 5547 3835
Size: 1712 3875
SfcI C'TryA_G
Cuts at: 826 1045 2563 2935 4070 4751 4942 826
Size: 219 1518 372 1135 681 191 1471
Fragments arranged by size:
1518 1471 1135 681 372 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5587
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5587
SmaI CCC'GGG
Cuts at: 1872 2914 1872
Size: 1042 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3691 4592 4854 820
Size: 197 926 1748 901 262 1553
Fragments arranged by size:
1748 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3378 493
Size: 2885 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5587
SphI G_CATG'C
Cuts at: 1132 2945 1132
Size: 1813 3774
SspI AAT'ATT
Cuts at: 5 52 3511 5
Size: 47 3459 2081
Fragments arranged by size:
3459 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5587
StyI C'CwwG_G
Cuts at: 513 1065 2004 2326 2877 3026 513
Size: 552 939 322 551 149 3074
Fragments arranged by size:
3074 939 552 551 322 149
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2237 3614 3631 3784 3969 4414 325
Size: 1255 657 1377 17 153 185 445 1498
Fragments arranged by size:
1498 1377 1255 657 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3833 5545 96
Size: 274 80 33 51 1443 1856 1712 138
Fragments arranged by size:
1856 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2686 2718 5547 5579 2686
Size: 32 2829 32 2694
Fragments arranged by size:
2829 2694 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2594 1316
Size: 1278 4309
XbaI T'CTAG_A
Cuts at: 2923 2923
Size: 5587
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5587
XmaI C'CCGG_G
Cuts at: 1870 2912 1870
Size: 1042 4545
XmnI GAAnn'nnTTC
Cuts at: 3716 3716
Size: 5587
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3395 276
Size: 53 83 186 936 1861 2468
Fragments arranged by size:
2468 1861 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SacII SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NarI
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SanDI SexAI SfiI SfoI SrfI
SwaI XcmI