Restriction map for
pFN21AB8406
(Circular) (Six-base) MAPSORT of: pFN21AB8406.seq Check: 7505 from: 1 to: 5488
pFN21AB8406 5488 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3298 278
Size: 53 83 186 936 1762 2468
Fragments arranged by size:
2468 1762 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2831 1937
Size: 894 4594
Acc65I G'GTAC_C
Cuts at: 2809 2809
Size: 5488
AclI AA'CG_TT
Cuts at: 1563 3615 3988 1563
Size: 2052 373 3063
Fragments arranged by size:
3063 2052 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2443 2611 2695 3551 4935 5203 1632
Size: 811 168 84 856 1384 268 1917
Fragments arranged by size:
1917 1384 856 811 268 168 84
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5291
AflIII A'CryG_T
Cuts at: 1203 4387 5364 1203
Size: 3184 977 1327
Fragments arranged by size:
3184 1327 977
AgeI A'CCGG_T
Cuts at: 4319 4319
Size: 5488
AhdI GACnn_n'nnGTC
Cuts at: 4217 4217
Size: 5488
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5488
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5488
AlwNI CAG_nnn'CTG
Cuts at: 2478 3231 4803 2478
Size: 753 1572 3163
Fragments arranged by size:
3163 1572 753
ApaLI G'TGCA_C
Cuts at: 3545 4701 3545
Size: 1156 4332
ApoI r'AATT_y
Cuts at: 1493 2033 2073 2313 2797 3023 3175 5284 1493
Size: 540 40 240 484 226 152 2109 1697
Fragments arranged by size:
2109 1697 540 484 240 226 152 40
AseI AT'TA_AT
Cuts at: 160 1032 4042 160
Size: 872 3010 1606
Fragments arranged by size:
3010 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5488
AvaI C'yCGr_G
Cuts at: 1870 1943 2813 1870
Size: 73 870 4545
Fragments arranged by size:
4545 870 73
BamHI G'GATC_C
Cuts at: 2818 3185 2818
Size: 367 5121
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2809 4264 618
Size: 325 217 627 1022 1455 1842
Fragments arranged by size:
1842 1455 1022 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2303 2807 1391
Size: 912 504 4072
Fragments arranged by size:
4072 912 504
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4517
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3679 3713 1903
Size: 34 1742 34 3678
Fragments arranged by size:
3678 1742 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3380 4590 3380
Size: 1210 4278
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5488
BfrBI ATG'CAT
Cuts at: 2065 2401 2485 2065
Size: 336 84 5068
Fragments arranged by size:
5068 336 84
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4099 136
Size: 107 122 71 631 605 2427 1525
Fragments arranged by size:
2427 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2053 5483 2053
Size: 3430 2058
BlpI GC'TnA_GC
Cuts at: 2905 2905
Size: 5488
Bme1580I G_kGCm'C
Cuts at: 1790 2021 3549 4705 1790
Size: 231 1528 1156 2573
Fragments arranged by size:
2573 1528 1156 231
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5488
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3219 4177 442
Size: 416 53 83 399 24 1802 958 1753
Fragments arranged by size:
1802 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5488
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2455 2607 4148 1239
Size: 1216 152 1541 2579
Fragments arranged by size:
2579 1541 1216 152
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3613 4478 4776 3613
Size: 865 298 4325
Fragments arranged by size:
4325 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2151 4151 882
Size: 617 652 2000 2219
Fragments arranged by size:
2219 2000 652 617
BsaAI yAC'GTr
Cuts at: 493 1204 3279 493
Size: 711 2075 2702
Fragments arranged by size:
2702 2075 711
BsaBI GATnn'nnATC
Cuts at: 5482 5482
Size: 5488
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3295 3677 4312 275
Size: 53 83 186 936 1762 382 635 1451
Fragments arranged by size:
1762 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2417 3921 4319 4593 4740 5151 2417
Size: 1504 398 274 147 411 2754
Fragments arranged by size:
2754 1504 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2160 2190 1178
Size: 30 165 30 757 30 4476
Fragments arranged by size:
4476 757 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5488
BseYI C'CCAG_C
Cuts at: 4691 5187 4691
Size: 496 4992
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4915
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2938 3699 4727 1540
Size: 275 186 937 761 1028 2301
Fragments arranged by size:
2301 1028 937 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2303 3549 3634 4705 2303
Size: 1246 85 1071 3086
Fragments arranged by size:
3086 1246 1071 85
BsmI GAATG_Cn'
Cuts at: 2999 3092 2999
Size: 93 5395
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2021 2303 2807 3549 3634 4705 1391
Size: 399 231 282 504 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 504 399 282 231 85
BspEI T'CCGG_A
Cuts at: 5151 5151
Size: 5488
BspHI T'CATG_A
Cuts at: 3377 5107 3377
Size: 1730 3758
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2843 844
Size: 1999 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3375 1394
Size: 429 1552 3507
Fragments arranged by size:
3507 1552 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3983 4157 65
Size: 1183 2735 174 1396
Fragments arranged by size:
2735 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4132 4319 1700
Size: 110 2322 187 2869
Fragments arranged by size:
2869 2322 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5488
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5488
BssSI C'ACGA_G
Cuts at: 3548 4560 3548
Size: 1012 4476
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5488
BstBI TT'CG_AA
Cuts at: 4292 4292
Size: 5488
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5488
BstYI r'GATC_y
Cuts at: 1972 2053 2818 3185 3570 3587 5039 5125 5137
Size: 81 765 367 385 17 1452 86 12
Cuts at: 5137 5483 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 765 385 367 346 86 81
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4936
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4749
Fragments arranged by size:
4749 683 56
BtsI GCAGTG_nn'
Cuts at: 3004 3798 3818 3004
Size: 794 20 4674
Fragments arranged by size:
4674 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5488
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5453
DraI TTT'AAA
Cuts at: 2434 2792 2434
Size: 358 5130
DraIII CAC_nnn'GTG
Cuts at: 2251 2251
Size: 5488
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4495 809
Size: 507 3179 1802
Fragments arranged by size:
3179 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2935 3824 5453
Size: 54 1425 50 275 18 1105 889 1629
Cuts at: 5453 8
Size: 43
Fragments arranged by size:
1629 1425 1105 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2935 1537
Size: 275 1123 4090
Fragments arranged by size:
4090 1123 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2436 3418 1353
Size: 1083 982 3423
Fragments arranged by size:
3423 1083 982
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4075 4449 4595 387
Size: 1064 138 2486 374 146 1280
Fragments arranged by size:
2486 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2301 2301
Size: 5488
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2443 2455 2607 2611 2695 3551 4148
Size: 393 811 12 152 4 84 856 597
Cuts at: 4148 4935 5203 1239
Size: 787 268 1524
Fragments arranged by size:
1524 856 811 787 597 393 268 152
84 12 4
EcoRI G'AATT_C
Cuts at: 2797 3175 2797
Size: 378 5110
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2140 2140
Size: 5488
FspI TGC'GCA
Cuts at: 3994 3994
Size: 5488
HaeII r_GCGC'y
Cuts at: 1678 4635 1678
Size: 2957 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2038 2070 2160 2192 2290
Size: 32 135 32 466 32 90 32 98
Cuts at: 2290 2322 2752 2784 4129 4161 4203 4235 1373
Size: 32 430 32 1345 32 42 32 2626
Fragments arranged by size:
2626 1345 466 430 135 98 90 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2832 3078 669
Size: 988 281 894 246 3079
Fragments arranged by size:
3079 988 894 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5488
HpaI GTT'AAC
Cuts at: 3078 3078
Size: 5488
KpnI G_GTAC'C
Cuts at: 2813 2813
Size: 5488
MfeI C'AATT_G
Cuts at: 2293 3087 2293
Size: 794 4694
MluI A'CGCG_T
Cuts at: 5364 5364
Size: 5488
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4602 4786 1305
Size: 3297 184 2007
Fragments arranged by size:
3297 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3666
Fragments arranged by size:
3666 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2100 2334 3446 3805 3964 518
Size: 648 934 234 1112 359 159 2042
Fragments arranged by size:
2042 1112 934 648 359 234 159
MspA1I CmG'CkG
Cuts at: 1606 2084 2904 3581 4729 4974 1606
Size: 478 820 677 1148 245 2120
Fragments arranged by size:
2120 1148 820 677 478 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4936
NdeI CA'TA_TG
Cuts at: 387 2319 387
Size: 1932 3556
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5488
NotI GC'GGCC_GC
Cuts at: 2935 2935
Size: 5488
NsiI A_TGCA'T
Cuts at: 2067 2403 2487 2067
Size: 336 84 5068
Fragments arranged by size:
5068 336 84
NspI r_CATG'y
Cuts at: 1132 1229 2401 2485 2846 4391 1132
Size: 97 1172 84 361 1545 2229
Fragments arranged by size:
2229 1545 1172 361 97 84
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5488
PciI A'CATG_T
Cuts at: 4387 4387
Size: 5488
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2251 1108
Size: 1143 4345
PmeI GTTT'AAAC
Cuts at: 2792 2792
Size: 5488
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3557 3589 5070 5102 3557
Size: 32 1481 32 3943
Fragments arranged by size:
3943 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5488
PsiI TTA'TAA
Cuts at: 3058 3058
Size: 5488
PstI C_TGCA'G
Cuts at: 830 2840 830
Size: 2010 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5488
PvuII CAG'CTG
Cuts at: 2084 2084
Size: 5488
SacI G_AGCT'C
Cuts at: 2303 2303
Size: 5488
SalI G'TCGA_C
Cuts at: 1936 2830 1936
Size: 894 4594
SbfI CC_TGCA'GG
Cuts at: 2840 2840
Size: 5488
ScaI AGT'ACT
Cuts at: 3736 5448 3736
Size: 1712 3776
SfcI C'TryA_G
Cuts at: 826 1045 2353 2599 2836 3971 4652 4843 826
Size: 219 1308 246 237 1135 681 191 1471
Fragments arranged by size:
1471 1308 1135 681 246 237 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5488
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5488
SmaI CCC'GGG
Cuts at: 1872 2815 1872
Size: 943 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3592 4493 4755 820
Size: 197 926 1649 901 262 1553
Fragments arranged by size:
1649 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3279 493
Size: 2786 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5488
SphI G_CATG'C
Cuts at: 1132 2401 2485 2846 1132
Size: 1269 84 361 3774
Fragments arranged by size:
3774 1269 361 84
SspI AAT'ATT
Cuts at: 5 52 3412 5
Size: 47 3360 2081
Fragments arranged by size:
3360 2081 47
StuI AGG'CCT
Cuts at: 1517 2456 2624 2708 1517
Size: 939 168 84 4297
Fragments arranged by size:
4297 939 168 84
StyI C'CwwG_G
Cuts at: 513 1065 2927 513
Size: 552 1862 3074
Fragments arranged by size:
3074 1862 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3515 3532 3685 3870 4315 325
Size: 1255 1935 17 153 185 445 1498
Fragments arranged by size:
1935 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3734 5446 96
Size: 274 80 33 51 1443 1757 1712 138
Fragments arranged by size:
1757 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5448 5480 5448
Size: 32 5456
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5488
XbaI T'CTAG_A
Cuts at: 2824 2824
Size: 5488
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5488
XmaI C'CCGG_G
Cuts at: 1870 2813 1870
Size: 943 4545
XmnI GAAnn'nnTTC
Cuts at: 2456 2540 2624 2681 3617 2456
Size: 84 84 57 936 4327
Fragments arranged by size:
4327 936 84 84 57
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3296 276
Size: 53 83 186 936 1762 2468
Fragments arranged by size:
2468 1762 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI