Restriction map for
pFN21AB8404
(Circular) (Six-base) MAPSORT of: pFN21AB8404.seq Check: 5066 from: 1 to: 8245
pFN21AB8404 8245 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2503 2819 2503
Size: 316 7929
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6055 278
Size: 53 83 186 936 4519 2468
Fragments arranged by size:
4519 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2292 3957 5588 1937
Size: 355 1665 1631 4594
Fragments arranged by size:
4594 1665 1631 355
Acc65I G'GTAC_C
Cuts at: 2657 5566 2657
Size: 2909 5336
AclI AA'CG_TT
Cuts at: 1563 4539 6372 6745 1563
Size: 2976 1833 373 3063
Fragments arranged by size:
3063 2976 1833 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 5492 6308 7692 7960 1632
Size: 3860 816 1384 268 1917
Fragments arranged by size:
3860 1917 1384 816 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8048
AflIII A'CryG_T
Cuts at: 1203 2851 3413 3942 4435 4568 7144 8121 1203
Size: 1648 562 529 493 133 2576 977 1327
Fragments arranged by size:
2576 1648 1327 977 562 529 493 133
AgeI A'CCGG_T
Cuts at: 7076 7076
Size: 8245
AhdI GACnn_n'nnGTC
Cuts at: 3558 6974 3558
Size: 3416 4829
AleI CACnn'nnGTG
Cuts at: 1166 2316 5015 5240 1166
Size: 1150 2699 225 4171
Fragments arranged by size:
4171 2699 1150 225
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2226 2334 4305 4936 5143 1184
Size: 1042 108 1971 631 207 4286
Fragments arranged by size:
4286 1971 1042 631 207 108
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2567 2599 2567
Size: 32 8213
AlwNI CAG_nnn'CTG
Cuts at: 2371 5090 5136 5988 7560 2371
Size: 2719 46 852 1572 3056
Fragments arranged by size:
3056 2719 1572 852 46
ApaI G_GGCC'C
Cuts at: 2954 3003 3159 4625 5276 2954
Size: 49 156 1466 651 5923
Fragments arranged by size:
5923 1466 651 156 49
ApaLI G'TGCA_C
Cuts at: 6302 7458 6302
Size: 1156 7089
ApoI r'AATT_y
Cuts at: 1493 5554 5780 5932 8041 1493
Size: 4061 226 152 2109 1697
Fragments arranged by size:
4061 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6799 160
Size: 872 5767 1606
Fragments arranged by size:
5767 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8245
AvaI C'yCGr_G
Cuts at: 1870 1943 3340 3978 4236 5276 5570 1870
Size: 73 1397 638 258 1040 294 4545
Fragments arranged by size:
4545 1397 1040 638 294 258 73
BamHI G'GATC_C
Cuts at: 4839 5575 5942 4839
Size: 736 367 7142
Fragments arranged by size:
7142 736 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2657 3242 3747 3830 4178
Size: 325 217 627 870 585 505 83 348
Cuts at: 4178 4501 4513 4600 4723 4851 5566 7021 618
Size: 323 12 87 123 128 715 1455 1842
Fragments arranged by size:
1842 1455 870 715 627 585 505 348
325 323 217 128 123 87 83 12
BanII G_rGCy'C
Cuts at: 1391 2670 2884 2941 2954 3003 3159 3458 3898
Size: 1279 214 57 13 49 156 299 440
Cuts at: 3898 4625 5153 5168 5195 5276 5564 1391
Size: 727 528 15 27 81 288 4072
Fragments arranged by size:
4072 1279 727 528 440 299 288 214
156 81 57 49 27 15 13
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2849 4454 928
Size: 971 950 1605 4719
Fragments arranged by size:
4719 1605 971 950
BbvCI CC'TCA_GC
Cuts at: 3732 3918 4376 3732
Size: 186 458 7601
Fragments arranged by size:
7601 458 186
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2797 2831 5318 5352 5490 5524 6436
Size: 34 860 34 2487 34 138 34 912
Cuts at: 6436 6470 1903
Size: 34 3678
Fragments arranged by size:
3678 2487 912 860 138 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3159 6137 7347 3159
Size: 2978 1210 4057
Fragments arranged by size:
4057 2978 1210
BclI T'GATC_A
Cuts at: 1548 3006 4794 1548
Size: 1458 1788 4999
Fragments arranged by size:
4999 1788 1458
BfrBI ATG'CAT
Cuts at: 4153 5530 4153
Size: 1377 6868
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4371 4732 6856
Size: 107 122 71 631 605 2699 361 2124
Cuts at: 6856 136
Size: 1525
Fragments arranged by size:
2699 2124 1525 631 605 361 122 107
71
BglII A'GATC_T
Cuts at: 8240 8240
Size: 8245
BlpI GC'TnA_GC
Cuts at: 3795 5662 3795
Size: 1867 6378
Bme1580I G_kGCm'C
Cuts at: 1790 2863 2954 3003 3159 4576 4605 4625 5267
Size: 1073 91 49 156 1417 29 20 642
Cuts at: 5267 5276 6306 7462 1790
Size: 9 1030 1156 2573
Fragments arranged by size:
2573 1417 1156 1073 1030 642 156 91
49 29 20 9
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8245
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2086 2121 2575
Size: 416 53 83 399 24 669 35 454
Cuts at: 2575 2614 2747 3101 3452 3517 3783 5976 6934
Size: 39 133 354 351 65 266 2193 958
Cuts at: 6934 442
Size: 1753
Fragments arranged by size:
2193 1753 958 669 454 416 399 354
351 266 133 83 65 53 39 35
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8245
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2851 2851
Size: 8245
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2094 2365 2431 3008 3217 5137 5179 5395
Size: 855 271 66 577 209 1920 42 216
Cuts at: 5395 6905 1239
Size: 1510 2579
Fragments arranged by size:
2579 1920 1510 855 577 271 216 209
66 42
Bpu10I CC'TnA_GC
Cuts at: 2652 3732 3918 4376 5147 2652
Size: 1080 186 458 771 5750
Fragments arranged by size:
5750 1080 771 458 186
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2165 3533 6370 7235 7533 2165
Size: 1368 2837 865 298 2877
Fragments arranged by size:
2877 2837 1368 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2570 2920 2960 3046 4039 6908 882
Size: 617 1071 350 40 86 993 2869 2219
Fragments arranged by size:
2869 2219 1071 993 617 350 86 40
BsaAI yAC'GTr
Cuts at: 493 1204 3943 4266 4438 4571 5013 6036 493
Size: 711 2739 323 172 133 442 1023 2702
Fragments arranged by size:
2739 2702 1023 711 442 323 172 133
BsaBI GATnn'nnATC
Cuts at: 4781 4799 8239 4781
Size: 18 3440 4787
Fragments arranged by size:
4787 3440 18
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2228 4812 6052 6434
Size: 53 83 186 936 695 2584 1240 382
Cuts at: 6434 7069 275
Size: 635 1451
Fragments arranged by size:
2584 1451 1240 936 695 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2033 2530 2884 6678 7076 7350 7497 7908 2033
Size: 497 354 3794 398 274 147 411 2370
Fragments arranged by size:
3794 2370 497 411 398 354 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2567 2597 4298 4328 1178
Size: 30 165 30 1164 30 1701 30 5095
Fragments arranged by size:
5095 1701 1164 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2495 3466 4787 1186
Size: 1309 971 1321 4644
Fragments arranged by size:
4644 1321 1309 971
BseYI C'CCAG_C
Cuts at: 2166 2644 3136 3391 3517 3890 4276 4416 4575
Size: 478 492 255 126 373 386 140 159
Cuts at: 4575 5023 5171 7448 7944 2166
Size: 448 148 2277 496 2467
Fragments arranged by size:
2467 2277 496 492 478 448 386 373
255 159 148 140 126
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2340 2696 3159 5267 1163
Size: 573 604 356 463 2108 4141
Fragments arranged by size:
4141 2108 604 573 463 356
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2059 5695 6456 7484 1540
Size: 275 186 58 3636 761 1028 2301
Fragments arranged by size:
3636 2301 1028 761 275 186 58
BsiHKAI G_wGCw'C
Cuts at: 4326 5153 6306 6391 7462 4326
Size: 827 1153 85 1071 5109
Fragments arranged by size:
5109 1153 1071 827 85
BsmI GAATG_Cn'
Cuts at: 4153 5756 5849 4153
Size: 1603 93 6549
Fragments arranged by size:
6549 1603 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2670 2863 2884 2941 2954 3003 3159
Size: 399 880 193 21 57 13 49 156
Cuts at: 3159 3458 3898 4326 4576 4605 4625 5153 5168
Size: 299 440 428 250 29 20 528 15
Cuts at: 5168 5195 5267 5276 5564 6306 6391 7462 1391
Size: 27 72 9 288 742 85 1071 2174
Fragments arranged by size:
2174 1071 880 742 528 440 428 399
299 288 250 193 156 85 72 57
49 29 27 21 20 15 13 9
BspEI T'CCGG_A
Cuts at: 2530 2884 7908 2530
Size: 354 5024 2867
Fragments arranged by size:
5024 2867 354
BspHI T'CATG_A
Cuts at: 3315 6134 7864 3315
Size: 2819 1730 3696
Fragments arranged by size:
3696 2819 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2503 2819 5600 844
Size: 1659 316 2781 3489
Fragments arranged by size:
3489 2781 1659 316
BsrBI CCG'CTC
Cuts at: 1394 1823 2120 6132 1394
Size: 429 297 4012 3507
Fragments arranged by size:
4012 3507 429 297
BsrDI GCAATG_nn'
Cuts at: 65 1248 2053 3404 6740 6914 65
Size: 1183 805 1351 3336 174 1396
Fragments arranged by size:
3336 1396 1351 1183 805 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2054 4520 6889 7076 1700
Size: 110 244 2466 2369 187 2869
Fragments arranged by size:
2869 2466 2369 244 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8245
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8245
BssSI C'ACGA_G
Cuts at: 2075 2171 6305 7317 2075
Size: 96 4134 1012 3003
Fragments arranged by size:
4134 3003 1012 96
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5136 794
Size: 4342 3903
BstBI TT'CG_AA
Cuts at: 7049 7049
Size: 8245
BstEII G'GTnAC_C
Cuts at: 1184 2731 3382 3466 1184
Size: 1547 651 84 5963
Fragments arranged by size:
5963 1547 651 84
BstXI CCAn_nnnn'nTGG
Cuts at: 2134 5242 2134
Size: 3108 5137
BstYI r'GATC_y
Cuts at: 1972 3210 4776 4839 5388 5575 5942 6327 6344
Size: 1238 1566 63 549 187 367 385 17
Cuts at: 6344 7796 7882 7894 8240 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1566 1452 1238 549 385 367 346
187 86 63 17 12
Bsu36I CC'TnA_GG
Cuts at: 3291 3634 3291
Size: 343 7902
BtgI C'CryG_G
Cuts at: 513 1065 3248 4429 4626 4816 4863 513
Size: 552 2183 1181 197 190 47 3895
Fragments arranged by size:
3895 2183 1181 552 197 190 47
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3365 5081 5321 487
Size: 683 56 2139 1716 240 3411
Fragments arranged by size:
3411 2139 1716 683 240 56
BtsI GCAGTG_nn'
Cuts at: 3885 4797 5289 5761 6555 6575 3885
Size: 912 492 472 794 20 5555
Fragments arranged by size:
5555 912 794 492 472 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8245
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8210
DraI TTT'AAA
Cuts at: 5549 5549
Size: 8245
DraIII CAC_nnn'GTG
Cuts at: 2094 2094
Size: 8245
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2603 2896 3965 4942 7252 809
Size: 507 1287 293 1069 977 2310 1802
Fragments arranged by size:
2310 1802 1287 1069 977 507 293
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2056 2391 3537
Size: 54 1425 50 275 18 226 335 1146
Cuts at: 3537 4053 4170 4522 4591 4648 5352 5511 5692
Size: 516 117 352 69 57 704 159 181
Cuts at: 5692 6581 8210 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1146 889 704 516 352 335
275 226 181 159 117 69 57 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2056 5692 1537
Size: 275 244 3636 4090
Fragments arranged by size:
4090 3636 275 244
EarI CTCTTCn'nnn_
Cuts at: 1353 2529 3314 6175 1353
Size: 1176 785 2861 3423
Fragments arranged by size:
3423 2861 1176 785
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2492 4918 5287 5359 5381 6832
Size: 1064 138 903 2426 369 72 22 1451
Cuts at: 6832 7206 7352 387
Size: 374 146 1280
Fragments arranged by size:
2426 1451 1280 1064 903 374 369 146
138 72 22
EcoICRI GAG'CTC
Cuts at: 5151 5151
Size: 8245
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2094 2365 2431 3008 3217 5137 5179
Size: 393 462 271 66 577 209 1920 42
Cuts at: 5179 5395 5492 6308 6905 7692 7960 1239
Size: 216 97 816 597 787 268 1524
Fragments arranged by size:
1920 1524 816 787 597 577 462 393
271 268 216 209 97 66 42
EcoNI CCTnn'n_nnAGG
Cuts at: 2214 3329 2214
Size: 1115 7130
EcoO109I rG'GnC_Cy
Cuts at: 2176 2681 2950 2951 2999 3155 3344 3778 4622
Size: 505 269 1 48 156 189 434 844
Cuts at: 4622 5272 5273 2176
Size: 650 1 5148
Fragments arranged by size:
5148 844 650 505 434 269 189 156
48 1 1
EcoRI G'AATT_C
Cuts at: 5554 5932 5554
Size: 378 7867
FspI TGC'GCA
Cuts at: 6751 6751
Size: 8245
HaeII r_GCGC'y
Cuts at: 1678 2156 2422 4205 4366 4383 5489 7392 1678
Size: 478 266 1783 161 17 1106 1903 2531
Fragments arranged by size:
2531 1903 1783 1106 478 266 161 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3544 3576 6886 6918 6960
Size: 32 135 32 1972 32 3310 32 42
Cuts at: 6960 6992 1373
Size: 32 2626
Fragments arranged by size:
3310 2626 1972 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5589 5835 669
Size: 988 281 3651 246 3079
Fragments arranged by size:
3651 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8245
HpaI GTT'AAC
Cuts at: 5835 5835
Size: 8245
KpnI G_GTAC'C
Cuts at: 2661 5570 2661
Size: 2909 5336
MfeI C'AATT_G
Cuts at: 5844 5844
Size: 8245
MluI A'CGCG_T
Cuts at: 8121 8121
Size: 8245
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3596 4371 7359 7543 1305
Size: 2291 775 2988 184 2007
Fragments arranged by size:
2988 2291 2007 775 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3539 4172 4650 10
Size: 54 1425 343 1707 633 478 3605
Fragments arranged by size:
3605 1707 1425 633 478 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2316 2564 3945 5015 5217 5240 6203
Size: 648 1150 248 1381 1070 202 23 963
Cuts at: 6203 6562 6721 518
Size: 359 159 2042
Fragments arranged by size:
2042 1381 1150 1070 963 648 359 248
202 159 23
MspA1I CmG'CkG
Cuts at: 1606 3794 3988 4063 4628 5308 5661 6338 7486
Size: 2188 194 75 565 680 353 677 1148
Cuts at: 7486 7731 1606
Size: 245 2120
Fragments arranged by size:
2188 2120 1148 680 677 565 353 245
194 75
NaeI GCC'GGC
Cuts at: 2056 2056
Size: 8245
NcoI C'CATG_G
Cuts at: 513 1065 4863 513
Size: 552 3798 3895
Fragments arranged by size:
3895 3798 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8245
NgoMIV G'CCGG_C
Cuts at: 2054 2054
Size: 8245
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8245
NotI GC'GGCC_GC
Cuts at: 5692 5692
Size: 8245
NsiI A_TGCA'T
Cuts at: 4155 5532 4155
Size: 1377 6868
NspI r_CATG'y
Cuts at: 1132 1229 3189 3261 3417 4536 5530 5603 7148
Size: 97 1960 72 156 1119 994 73 1545
Cuts at: 7148 1132
Size: 2229
Fragments arranged by size:
2229 1960 1545 1119 994 156 97 73
72
PasI CC'CwG_GG
Cuts at: 1348 2947 3783 4389 1348
Size: 1599 836 606 5204
Fragments arranged by size:
5204 1599 836 606
PciI A'CATG_T
Cuts at: 3413 7144 3413
Size: 3731 4514
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2386 1108
Size: 1278 6967
PfoI T'CCnGG_A
Cuts at: 2133 2403 3766 3902 5064 5153 2133
Size: 270 1363 136 1162 89 5225
Fragments arranged by size:
5225 1363 1162 270 136 89
PmeI GTTT'AAAC
Cuts at: 5549 5549
Size: 8245
PmlI CAC'GTG
Cuts at: 3943 4266 4438 4571 5013 3943
Size: 323 172 133 442 7175
Fragments arranged by size:
7175 442 323 172 133
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2567 2599 6314 6346 7827 7859 2567
Size: 32 3715 32 1481 32 2953
Fragments arranged by size:
3715 2953 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2681 3778 2681
Size: 1097 7148
PshAI GACnn'nnGTC
Cuts at: 1613 2620 4137 1613
Size: 1007 1517 5721
Fragments arranged by size:
5721 1517 1007
PsiI TTA'TAA
Cuts at: 5815 5815
Size: 8245
PspOMI G'GGCC_C
Cuts at: 2950 2999 3155 4621 5272 2950
Size: 49 156 1466 651 5923
Fragments arranged by size:
5923 1466 651 156 49
PstI C_TGCA'G
Cuts at: 830 2615 2640 3793 4771 4925 5597 830
Size: 1785 25 1153 978 154 672 3478
Fragments arranged by size:
3478 1785 1153 978 672 154 25
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8245
PvuII CAG'CTG
Cuts at: 3794 3988 5308 3794
Size: 194 1320 6731
Fragments arranged by size:
6731 1320 194
RsrII CG'GwC_CG
Cuts at: 4737 4737
Size: 8245
SacI G_AGCT'C
Cuts at: 5153 5153
Size: 8245
SacII CC_GC'GG
Cuts at: 4629 4629
Size: 8245
SalI G'TCGA_C
Cuts at: 1936 5587 1936
Size: 3651 4594
SanDI GG'GwC_CC
Cuts at: 3778 3778
Size: 8245
SapI GCTCTTCn'nnn_
Cuts at: 3314 3314
Size: 8245
SbfI CC_TGCA'GG
Cuts at: 5597 5597
Size: 8245
ScaI AGT'ACT
Cuts at: 6493 8205 6493
Size: 1712 6533
SfcI C'TryA_G
Cuts at: 826 1045 2067 2611 2636 3789 3954 4767 4921
Size: 219 1022 544 25 1153 165 813 154
Cuts at: 4921 4960 5593 6728 7409 7600 826
Size: 39 633 1135 681 191 1471
Fragments arranged by size:
1471 1153 1135 1022 813 681 633 544
219 191 165 154 39 25
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8245
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8245
SmaI CCC'GGG
Cuts at: 1872 3342 3980 4238 5278 5572 1872
Size: 1470 638 258 1040 294 4545
Fragments arranged by size:
4545 1470 1040 638 294 258
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2180 3548 6349 7250 7512 820
Size: 197 926 237 1368 2801 901 262 1553
Fragments arranged by size:
2801 1553 1368 926 901 262 237 197
SnaBI TAC'GTA
Cuts at: 493 6036 493
Size: 5543 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8245
SphI G_CATG'C
Cuts at: 1132 5603 1132
Size: 4471 3774
SspI AAT'ATT
Cuts at: 5 52 6169 5
Size: 47 6117 2081
Fragments arranged by size:
6117 2081 47
StuI AGG'CCT
Cuts at: 1517 2273 2411 4073 1517
Size: 756 138 1662 5689
Fragments arranged by size:
5689 1662 756 138
StyI C'CwwG_G
Cuts at: 513 1065 2187 2394 3660 3666 4068 4338 4863
Size: 552 1122 207 1266 6 402 270 525
Cuts at: 4863 5684 513
Size: 821 3074
Fragments arranged by size:
3074 1266 1122 821 552 525 402 270
207 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2105 3109 3673 3848 4325 4531 5005
Size: 1255 525 1004 564 175 477 206 474
Cuts at: 5005 5067 6272 6289 6442 6627 7072 325
Size: 62 1205 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1205 1004 564 525 477 474
445 206 185 175 153 62 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6491 8203 96
Size: 274 80 33 51 1443 4514 1712 138
Fragments arranged by size:
4514 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8205 8237 8205
Size: 32 8213
Tth111I GACn'n_nGTC
Cuts at: 1316 2227 4231 1316
Size: 911 2004 5330
Fragments arranged by size:
5330 2004 911
XbaI T'CTAG_A
Cuts at: 5581 5581
Size: 8245
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2455 3877 4367 2455
Size: 1422 490 6333
Fragments arranged by size:
6333 1422 490
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8245
XmaI C'CCGG_G
Cuts at: 1870 3340 3978 4236 5276 5570 1870
Size: 1470 638 258 1040 294 4545
Fragments arranged by size:
4545 1470 1040 638 294 258
XmnI GAAnn'nnTTC
Cuts at: 6374 6374
Size: 8245
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6053 276
Size: 53 83 186 936 4519 2468
Fragments arranged by size:
4519 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BsiWI BsmBI BstZ17I
EcoRV FalI FseI FspAI KasI NarI NruI PacI
PspXI PsrI SexAI SfiI SfoI SrfI SwaI