Restriction map for
pFN21AB8401
(Circular) (Six-base) MAPSORT of: pFN21AB8401.seq Check: 4912 from: 1 to: 7564
pFN21AB8401 7564 bp
With 182 enzymes: SgfI *
August 16, 2010 14:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5374 278
Size: 53 83 186 936 3838 2468
Fragments arranged by size:
3838 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4907 1937
Size: 2970 4594
Acc65I G'GTAC_C
Cuts at: 3560 4885 3560
Size: 1325 6239
AclI AA'CG_TT
Cuts at: 1563 5691 6064 1563
Size: 4128 373 3063
Fragments arranged by size:
4128 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2142 2195 3104 3531 5627 7011 7279 1632
Size: 510 53 909 427 2096 1384 268 1917
Fragments arranged by size:
2096 1917 1384 909 510 427 268 53
AfeI AGC'GCT
Cuts at: 3293 3293
Size: 7564
AflII C'TTAA_G
Cuts at: 820 1017 2629 4816 820
Size: 197 1612 2187 3568
Fragments arranged by size:
3568 2187 1612 197
AflIII A'CryG_T
Cuts at: 1203 2031 3927 6463 7440 1203
Size: 828 1896 2536 977 1327
Fragments arranged by size:
2536 1896 1327 977 828
AgeI A'CCGG_T
Cuts at: 6395 6395
Size: 7564
AhdI GACnn_n'nnGTC
Cuts at: 6293 6293
Size: 7564
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7564
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4050 1184
Size: 2866 4698
AlwNI CAG_nnn'CTG
Cuts at: 2965 3254 3257 4178 4766 5307 6879 2965
Size: 289 3 921 588 541 1572 3650
Fragments arranged by size:
3650 1572 921 588 541 289 3
ApaLI G'TGCA_C
Cuts at: 2823 5621 6777 2823
Size: 2798 1156 3610
Fragments arranged by size:
3610 2798 1156
ApoI r'AATT_y
Cuts at: 1493 2345 2497 3224 3739 3840 4520 4541 4788
Size: 852 152 727 515 101 680 21 247
Cuts at: 4788 4873 5099 5251 7360 1493
Size: 85 226 152 2109 1697
Fragments arranged by size:
2109 1697 852 727 680 515 247 226
152 152 101 85 21
AseI AT'TA_AT
Cuts at: 160 1032 6118 160
Size: 872 5086 1606
Fragments arranged by size:
5086 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7564
AvaI C'yCGr_G
Cuts at: 1870 1943 4889 1870
Size: 73 2946 4545
Fragments arranged by size:
4545 2946 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3388 3421 3388
Size: 33 7531
BamHI G'GATC_C
Cuts at: 3384 4894 5261 3384
Size: 1510 367 5687
Fragments arranged by size:
5687 1510 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3560 4885 6340 618
Size: 325 217 627 1773 1325 1455 1842
Fragments arranged by size:
1842 1773 1455 1325 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2734 4883 1391
Size: 1343 2149 4072
Fragments arranged by size:
4072 2149 1343
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3420 3621 928
Size: 971 1521 201 4871
Fragments arranged by size:
4871 1521 971 201
BbvCI CC'TCA_GC
Cuts at: 3753 3753
Size: 7564
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5755 5789 1903
Size: 34 3818 34 3678
Fragments arranged by size:
3818 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5456 6666 5456
Size: 1210 6354
BclI T'GATC_A
Cuts at: 1548 2644 1548
Size: 1096 6468
BfrBI ATG'CAT
Cuts at: 2315 2582 2315
Size: 267 7297
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 6175 136
Size: 107 122 71 631 605 337 4166 1525
Fragments arranged by size:
4166 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 7559 7559
Size: 7564
BlpI GC'TnA_GC
Cuts at: 4981 4981
Size: 7564
Bme1580I G_kGCm'C
Cuts at: 1790 2827 3583 5625 6781 1790
Size: 1037 756 2042 1156 2573
Fragments arranged by size:
2573 2042 1156 1037 756
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7564
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5295 6253 442
Size: 416 53 83 399 24 3878 958 1753
Fragments arranged by size:
3878 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7564
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2256 4449 4626 6224 1239
Size: 1017 2193 177 1598 2579
Fragments arranged by size:
2579 2193 1598 1017 177
Bpu10I CC'TnA_GC
Cuts at: 3584 3753 3584
Size: 169 7395
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3649 3839 5689 6554 6852 3649
Size: 190 1850 865 298 4361
Fragments arranged by size:
4361 1850 865 298 190
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6227 882
Size: 617 4728 2219
Fragments arranged by size:
4728 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5355 493
Size: 711 4151 2702
Fragments arranged by size:
4151 2702 711
BsaBI GATnn'nnATC
Cuts at: 2186 2610 3834 7558 2186
Size: 424 1224 3724 2192
Fragments arranged by size:
3724 2192 1224 424
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5371 5753 6388 275
Size: 53 83 186 936 3838 382 635 1451
Fragments arranged by size:
3838 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5997 6395 6669 6816 7227 5997
Size: 398 274 147 411 6334
Fragments arranged by size:
6334 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4321 4351 1178
Size: 30 165 30 2918 30 4391
Fragments arranged by size:
4391 2918 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3387 4329 1186
Size: 2201 942 4421
Fragments arranged by size:
4421 2201 942
BseYI C'CCAG_C
Cuts at: 2798 6767 7263 2798
Size: 3969 496 3099
Fragments arranged by size:
3969 3099 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6991
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5014 5775 6803 1540
Size: 275 186 3013 761 1028 2301
Fragments arranged by size:
3013 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2734 2827 2996 5625 5710 6781 2734
Size: 93 169 2629 85 1071 3517
Fragments arranged by size:
3517 2629 1071 169 93 85
BsmI GAATG_Cn'
Cuts at: 2584 3166 3315 3911 5075 5168 2584
Size: 582 149 596 1164 93 4980
Fragments arranged by size:
4980 1164 596 582 149 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4326 4326
Size: 7564
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2734 2827 2996 3583 4883 5625 5710
Size: 399 944 93 169 587 1300 742 85
Cuts at: 5710 6781 1391
Size: 1071 2174
Fragments arranged by size:
2174 1300 1071 944 742 587 399 169
93 85
BspEI T'CCGG_A
Cuts at: 7227 7227
Size: 7564
BspHI T'CATG_A
Cuts at: 2944 4342 5453 7183 2944
Size: 1398 1111 1730 3325
Fragments arranged by size:
3325 1730 1398 1111
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3435 4919 844
Size: 2591 1484 3489
Fragments arranged by size:
3489 2591 1484
BsrBI CCG'CTC
Cuts at: 1394 1823 5451 1394
Size: 429 3628 3507
Fragments arranged by size:
3628 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3161 3698 4098 6059 6233 65
Size: 1183 1913 537 400 1961 174 1396
Fragments arranged by size:
1961 1913 1396 1183 537 400 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4775 6208 6395 1700
Size: 110 2965 1433 187 2869
Fragments arranged by size:
2965 2869 1433 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7564
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7564
BssSI C'ACGA_G
Cuts at: 5624 6636 5624
Size: 1012 6552
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2831 3254 794
Size: 2037 423 5104
Fragments arranged by size:
5104 2037 423
BstBI TT'CG_AA
Cuts at: 6368 6368
Size: 7564
BstEII G'GTnAC_C
Cuts at: 1184 4150 1184
Size: 2966 4598
BstYI r'GATC_y
Cuts at: 1972 2326 2357 2962 3040 3384 3438 3459 4123
Size: 354 31 605 78 344 54 21 664
Cuts at: 4123 4174 4297 4894 5261 5646 5663 7115 7201
Size: 51 123 597 367 385 17 1452 86
Cuts at: 7201 7213 7559 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 664 605 597 385 367 354
346 344 123 86 78 54 51 31
21 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 7012
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6825
Fragments arranged by size:
6825 683 56
BtsI GCAGTG_nn'
Cuts at: 5080 5874 5894 5080
Size: 794 20 6750
Fragments arranged by size:
6750 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7564
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7529
DraI TTT'AAA
Cuts at: 4590 4868 4590
Size: 278 7286
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6571 809
Size: 507 5255 1802
Fragments arranged by size:
5255 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2475 4777 5011
Size: 54 1425 50 275 18 645 2302 234
Cuts at: 5011 5900 7529 8
Size: 889 1629 43
Fragments arranged by size:
2302 1629 1425 889 645 275 234 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5011 1537
Size: 275 3199 4090
Fragments arranged by size:
4090 3199 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2214 2370 3055 3060 3807 3945 5494 1353
Size: 861 156 685 5 747 138 1549 3423
Fragments arranged by size:
3423 1549 861 747 685 156 138 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6151 6525 6671 387
Size: 1064 138 4562 374 146 1280
Fragments arranged by size:
4562 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2732 2732
Size: 7564
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2142 2195 2256 3104 3531 4449 4626
Size: 393 510 53 61 848 427 918 177
Cuts at: 4626 5627 6224 7011 7279 1239
Size: 1001 597 787 268 1524
Fragments arranged by size:
1524 1001 918 848 787 597 510 427
393 268 177 61 53
EcoNI CCTnn'n_nnAGG
Cuts at: 3086 3086
Size: 7564
EcoO109I rG'GnC_Cy
Cuts at: 3447 4651 3447
Size: 1204 6360
EcoRI G'AATT_C
Cuts at: 4520 4788 4873 5251 4520
Size: 268 85 378 6833
Fragments arranged by size:
6833 378 268 85
EcoRV GAT'ATC
Cuts at: 2977 2977
Size: 7564
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4736 4736
Size: 7564
FspI TGC'GCA
Cuts at: 6070 6070
Size: 7564
HaeII r_GCGC'y
Cuts at: 1678 3295 6711 1678
Size: 1617 3416 2531
Fragments arranged by size:
3416 2531 1617
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2096 2128 3032 3064 3944
Size: 32 135 32 524 32 904 32 880
Cuts at: 3944 3976 4321 4353 4818 4850 6205 6237 6279
Size: 32 345 32 465 32 1355 32 42
Cuts at: 6279 6311 1373
Size: 32 2626
Fragments arranged by size:
2626 1355 904 880 524 465 345 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4207 4570 4620 4908 5154 669
Size: 988 281 2269 363 50 288 246 3079
Fragments arranged by size:
3079 2269 988 363 288 281 246 50
HindIII A'AGCT_T
Cuts at: 748 2124 4191 748
Size: 1376 2067 4121
Fragments arranged by size:
4121 2067 1376
HpaI GTT'AAC
Cuts at: 4570 5154 4570
Size: 584 6980
KpnI G_GTAC'C
Cuts at: 3564 4889 3564
Size: 1325 6239
MfeI C'AATT_G
Cuts at: 3465 4157 5163 3465
Size: 692 1006 5866
Fragments arranged by size:
5866 1006 692
MluI A'CGCG_T
Cuts at: 7440 7440
Size: 7564
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6678 6862 1305
Size: 5373 184 2007
Fragments arranged by size:
5373 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2477 10
Size: 54 1425 343 645 5097
Fragments arranged by size:
5097 1425 645 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2340 2451 4577 5522 5881 6040 518
Size: 648 1174 111 2126 945 359 159 2042
Fragments arranged by size:
2126 2042 1174 945 648 359 159 111
MspA1I CmG'CkG
Cuts at: 1606 2677 3254 4075 4980 5657 6805 7050 1606
Size: 1071 577 821 905 677 1148 245 2120
Fragments arranged by size:
2120 1148 1071 905 821 677 577 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7012
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7564
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7564
NotI GC'GGCC_GC
Cuts at: 5011 5011
Size: 7564
NsiI A_TGCA'T
Cuts at: 2317 2584 2317
Size: 267 7297
NspI r_CATG'y
Cuts at: 1132 1229 2433 3931 4922 6467 1132
Size: 97 1204 1498 991 1545 2229
Fragments arranged by size:
2229 1545 1498 1204 991 97
PacI TTA_AT'TAA
Cuts at: 2040 2040
Size: 7564
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7564
PciI A'CATG_T
Cuts at: 3927 6463 3927
Size: 2536 5028
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4810 1108
Size: 3702 3862
PfoI T'CCnGG_A
Cuts at: 2170 4224 2170
Size: 2054 5510
PmeI GTTT'AAAC
Cuts at: 4868 4868
Size: 7564
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5633 5665 7146 7178 5633
Size: 32 1481 32 6019
Fragments arranged by size:
6019 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3447 3447
Size: 7564
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7564
PsiI TTA'TAA
Cuts at: 2700 5134 2700
Size: 2434 5130
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2012 2044 2496 2528 2940 2972 3519 3551 3702
Size: 32 452 32 412 32 547 32 151
Cuts at: 3702 3734 2012
Size: 32 5842
Fragments arranged by size:
5842 547 452 412 151 32 32 32
32 32
PstI C_TGCA'G
Cuts at: 830 2676 3030 4916 830
Size: 1846 354 1886 3478
Fragments arranged by size:
3478 1886 1846 354
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7564
PvuII CAG'CTG
Cuts at: 2677 3254 4075 2677
Size: 577 821 6166
Fragments arranged by size:
6166 821 577
SacI G_AGCT'C
Cuts at: 2734 2734
Size: 7564
SalI G'TCGA_C
Cuts at: 1936 4906 1936
Size: 2970 4594
SapI GCTCTTCn'nnn_
Cuts at: 3060 3060
Size: 7564
SbfI CC_TGCA'GG
Cuts at: 4916 4916
Size: 7564
ScaI AGT'ACT
Cuts at: 2635 2955 5812 7524 2635
Size: 320 2857 1712 2675
Fragments arranged by size:
2857 2675 1712 320
SfcI C'TryA_G
Cuts at: 826 1045 2491 2672 2957 3026 3400 3525 4912
Size: 219 1446 181 285 69 374 125 1387
Cuts at: 4912 6047 6728 6919 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1446 1387 1135 681 374 285 219
191 181 125 69
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7564
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7564
SmaI CCC'GGG
Cuts at: 1872 4891 1872
Size: 3019 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2629 3628 3854 4816 5668 6569
Size: 197 926 686 999 226 962 852 901
Cuts at: 6569 6831 820
Size: 262 1553
Fragments arranged by size:
1553 999 962 926 901 852 686 262
226 197
SnaBI TAC'GTA
Cuts at: 493 5355 493
Size: 4862 2702
SpeI A'CTAG_T
Cuts at: 152 4856 152
Size: 4704 2860
SphI G_CATG'C
Cuts at: 1132 4922 1132
Size: 3790 3774
SspI AAT'ATT
Cuts at: 5 52 4357 5488 5
Size: 47 4305 1131 2081
Fragments arranged by size:
4305 2081 1131 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7564
StyI C'CwwG_G
Cuts at: 513 1065 3408 3451 5003 513
Size: 552 2343 43 1552 3074
Fragments arranged by size:
3074 2343 1552 552 43
SwaI ATTT'AAAT
Cuts at: 4590 4590
Size: 7564
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4169 5591 5608 5761 5946 6391 325
Size: 1255 2589 1422 17 153 185 445 1498
Fragments arranged by size:
2589 1498 1422 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2633 2953 3997
Size: 274 80 33 51 1443 656 320 1044
Cuts at: 3997 4231 5810 7522 96
Size: 234 1579 1712 138
Fragments arranged by size:
1712 1579 1443 1044 656 320 274 234
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7524 7556 7524
Size: 32 7532
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7564
XbaI T'CTAG_A
Cuts at: 2092 3053 3593 4900 2092
Size: 961 540 1307 4756
Fragments arranged by size:
4756 1307 961 540
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7564
XmaI C'CCGG_G
Cuts at: 1870 4889 1870
Size: 3019 4545
XmnI GAAnn'nnTTC
Cuts at: 2166 2256 2349 3285 3339 3417 5693 2166
Size: 90 93 936 54 78 2276 4037
Fragments arranged by size:
4037 2276 936 93 90 78 54
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5372 276
Size: 53 83 186 936 3838 2468
Fragments arranged by size:
3838 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PacI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PsrI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI SwaI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BbeI BplI BsiWI
BstXI BstZ17I Bsu36I DraIII FseI FspAI KasI NaeI
NarI NgoMIV NruI PmlI PspOMI PspXI RsrII SacII
SanDI SexAI SfiI SfoI SrfI XcmI