Restriction map for
pFN21AB8398
(Circular) (Six-base) MAPSORT of: pFN21AB8398.seq Check: 1169 from: 1 to: 6589
pFN21AB8398 6589 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4399 278
Size: 53 83 186 936 2863 2468
Fragments arranged by size:
2863 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3932 1937
Size: 1995 4594
Acc65I G'GTAC_C
Cuts at: 3910 3910
Size: 6589
AclI AA'CG_TT
Cuts at: 1563 4716 5089 1563
Size: 3153 373 3063
Fragments arranged by size:
3153 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3462 4652 6036 6304 1632
Size: 1830 1190 1384 268 1917
Fragments arranged by size:
1917 1830 1384 1190 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6392
AflIII A'CryG_T
Cuts at: 1203 3432 5488 6465 1203
Size: 2229 2056 977 1327
Fragments arranged by size:
2229 2056 1327 977
AgeI A'CCGG_T
Cuts at: 5420 5420
Size: 6589
AhdI GACnn_n'nnGTC
Cuts at: 2704 5318 2704
Size: 2614 3975
AleI CACnn'nnGTG
Cuts at: 1166 2933 1166
Size: 1767 4822
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2636 1184
Size: 1452 5137
AlwNI CAG_nnn'CTG
Cuts at: 2014 2904 3257 3629 3865 4332 5904 2014
Size: 890 353 372 236 467 1572 2699
Fragments arranged by size:
2699 1572 890 467 372 353 236
ApaI G_GGCC'C
Cuts at: 3767 3767
Size: 6589
ApaLI G'TGCA_C
Cuts at: 2492 4646 5802 2492
Size: 2154 1156 3279
Fragments arranged by size:
3279 2154 1156
ApoI r'AATT_y
Cuts at: 1493 3898 4124 4276 6385 1493
Size: 2405 226 152 2109 1697
Fragments arranged by size:
2405 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5143 160
Size: 872 4111 1606
Fragments arranged by size:
4111 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6589
AvaI C'yCGr_G
Cuts at: 1870 1943 2224 2931 3392 3606 3914 1870
Size: 73 281 707 461 214 308 4545
Fragments arranged by size:
4545 707 461 308 281 214 73
BamHI G'GATC_C
Cuts at: 3448 3796 3919 4286 3448
Size: 348 123 367 5751
Fragments arranged by size:
5751 367 348 123
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2130 2154 2189 2229 2741
Size: 325 217 627 343 24 35 40 512
Cuts at: 2741 2967 3910 5365 618
Size: 226 943 1455 1842
Fragments arranged by size:
1842 1455 943 627 512 343 325 226
217 40 35 24
BanII G_rGCy'C
Cuts at: 1391 2225 2642 3291 3311 3399 3417 3549 3702
Size: 834 417 649 20 88 18 132 153
Cuts at: 3702 3753 3767 3908 1391
Size: 51 14 141 4072
Fragments arranged by size:
4072 834 649 417 153 141 132 88
51 20 18 14
BbeI G_GCGC'C
Cuts at: 2233 2233
Size: 6589
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5618
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2359 2393 4780 4814 1903
Size: 34 50 34 338 34 2387 34 3678
Fragments arranged by size:
3678 2387 338 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4481 5691 4481
Size: 1210 5379
BclI T'GATC_A
Cuts at: 1548 2085 1548
Size: 537 6052
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2999 3688 5200
Size: 107 122 71 631 605 1327 689 1512
Cuts at: 5200 136
Size: 1525
Fragments arranged by size:
1525 1512 1327 689 631 605 122 107
71
BglII A'GATC_T
Cuts at: 2281 2527 6584 2281
Size: 246 4057 2286
Fragments arranged by size:
4057 2286 246
BlpI GC'TnA_GC
Cuts at: 3290 4006 3290
Size: 716 5873
Bme1580I G_kGCm'C
Cuts at: 1790 2496 2970 3767 4650 5806 1790
Size: 706 474 797 883 1156 2573
Fragments arranged by size:
2573 1156 883 797 706 474
BmgBI CAC'GTC
Cuts at: 1318 3433 1318
Size: 2115 4474
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2926 3151 3393
Size: 416 53 83 399 24 1509 225 242
Cuts at: 3393 4320 5278 442
Size: 927 958 1753
Fragments arranged by size:
1753 1509 958 927 416 399 242 225
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2063 1056
Size: 1007 5582
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3772 3772
Size: 6589
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2192 2558 2906 3056 3128 5249 1239
Size: 953 366 348 150 72 2121 2579
Fragments arranged by size:
2579 2121 953 366 348 150 72
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2516 3110 3200 3221 3335 3764 4714 5579 5877
Size: 594 90 21 114 429 950 865 298
Cuts at: 5877 2516
Size: 3228
Fragments arranged by size:
3228 950 865 594 429 298 114 90
21
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2168 5252 882
Size: 617 669 3084 2219
Fragments arranged by size:
3084 2219 669 617
BsaAI yAC'GTr
Cuts at: 493 1204 4380 493
Size: 711 3176 2702
Fragments arranged by size:
3176 2702 711
BsaBI GATnn'nnATC
Cuts at: 2309 2381 6583 2309
Size: 72 4202 2315
Fragments arranged by size:
4202 2315 72
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2230 4396 4778 5413
Size: 53 83 186 936 697 2166 382 635
Cuts at: 5413 275
Size: 1451
Fragments arranged by size:
2166 1451 936 697 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2253 2844 3283 5022 5420 5694 5841 6252 2253
Size: 591 439 1739 398 274 147 411 2590
Fragments arranged by size:
2590 1739 591 439 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6364
Fragments arranged by size:
6364 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2711 3376 3479 3761 1186
Size: 1525 665 103 282 4014
Fragments arranged by size:
4014 1525 665 282 103
BseYI C'CCAG_C
Cuts at: 5792 6288 5792
Size: 496 6093
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3076 1163
Size: 573 1340 4676
Fragments arranged by size:
4676 1340 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2052 2069 4039 4800 5828 1540
Size: 275 186 51 17 1970 761 1028 2301
Fragments arranged by size:
2301 1970 1028 761 275 186 51 17
BsiHKAI G_wGCw'C
Cuts at: 2225 2496 3291 3417 3549 4650 4735 5806 2225
Size: 271 795 126 132 1101 85 1071 3008
Fragments arranged by size:
3008 1101 1071 795 271 132 126 85
BsmI GAATG_Cn'
Cuts at: 3118 3231 3345 4100 4193 3118
Size: 113 114 755 93 5514
Fragments arranged by size:
5514 755 114 113 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2488 2488
Size: 6589
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2225 2496 2642 2970 3291 3311 3399
Size: 399 435 271 146 328 321 20 88
Cuts at: 3399 3417 3549 3702 3753 3767 3908 4650 4735
Size: 18 132 153 51 14 141 742 85
Cuts at: 4735 5806 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 435 399 328 321 271
153 146 141 132 88 85 51 20
18 14
BspEI T'CCGG_A
Cuts at: 2253 3283 6252 2253
Size: 1030 2969 2590
Fragments arranged by size:
2969 2590 1030
BspHI T'CATG_A
Cuts at: 4478 6208 4478
Size: 1730 4859
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2157 3944 844
Size: 1313 1787 3489
Fragments arranged by size:
3489 1787 1313
BsrBI CCG'CTC
Cuts at: 1394 1823 2434 4476 1394
Size: 429 611 2042 3507
Fragments arranged by size:
3507 2042 611 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2340 5084 5258 65
Size: 1183 1092 2744 174 1396
Fragments arranged by size:
2744 1396 1183 1092 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5233 5420 1700
Size: 110 3423 187 2869
Fragments arranged by size:
3423 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6589
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6589
BssSI C'ACGA_G
Cuts at: 4649 5661 4649
Size: 1012 5577
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2149 794
Size: 1355 5234
BstBI TT'CG_AA
Cuts at: 5393 5393
Size: 6589
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6589
BstXI CCAn_nnnn'nTGG
Cuts at: 2935 3200 2935
Size: 265 6324
BstYI r'GATC_y
Cuts at: 1972 2281 2527 2772 3448 3796 3919 4286 4671
Size: 309 246 245 676 348 123 367 385
Cuts at: 4671 4688 6140 6226 6238 6584 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 676 385 367 348 346 309
246 245 123 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3622 3779 3622
Size: 157 6432
BtgI C'CryG_G
Cuts at: 513 1065 2997 513
Size: 552 1932 4105
Fragments arranged by size:
4105 1932 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3179 3479 487
Size: 683 56 1953 300 3597
Fragments arranged by size:
3597 1953 683 300 56
BtsI GCAGTG_nn'
Cuts at: 2983 3186 3704 4105 4899 4919 2983
Size: 203 518 401 794 20 4653
Fragments arranged by size:
4653 794 518 401 203 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6589
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6554
DraI TTT'AAA
Cuts at: 3893 3893
Size: 6589
DraIII CAC_nnn'GTG
Cuts at: 3083 3083
Size: 6589
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5596 809
Size: 507 4280 1802
Fragments arranged by size:
4280 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2049 2066 2238
Size: 54 1425 50 275 18 219 17 172
Cuts at: 2238 2895 4036 4925 6554 8
Size: 657 1141 889 1629 43
Fragments arranged by size:
1629 1425 1141 889 657 275 219 172
54 50 43 18 17
EagI C'GGCC_G
Cuts at: 1537 1812 2049 2066 4036 1537
Size: 275 237 17 1970 4090
Fragments arranged by size:
4090 1970 275 237 17
EarI CTCTTCn'nnn_
Cuts at: 1353 4519 1353
Size: 3166 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2090 5176 5550 5696 387
Size: 1064 138 501 3086 374 146 1280
Fragments arranged by size:
3086 1280 1064 501 374 146 138
EcoICRI GAG'CTC
Cuts at: 2223 3289 3415 3547 2223
Size: 1066 126 132 5265
Fragments arranged by size:
5265 1066 132 126
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2192 2558 2906 3056 3128 3462 4652
Size: 393 560 366 348 150 72 334 1190
Cuts at: 4652 5249 6036 6304 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1190 787 597 560 393 366 348
334 268 150 72
EcoO109I rG'GnC_Cy
Cuts at: 2799 3277 3763 3764 2799
Size: 478 486 1 5624
Fragments arranged by size:
5624 486 478 1
EcoRI G'AATT_C
Cuts at: 3898 4276 3898
Size: 378 6211
FspI TGC'GCA
Cuts at: 5095 5095
Size: 6589
HaeII r_GCGC'y
Cuts at: 1678 2032 2233 3115 3787 5736 1678
Size: 354 201 882 672 1949 2531
Fragments arranged by size:
2531 1949 882 672 354 201
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2300 2332 2690 2722 3433
Size: 32 135 32 728 32 358 32 711
Cuts at: 3433 3465 5230 5262 5304 5336 1373
Size: 32 1765 32 42 32 2626
Fragments arranged by size:
2626 1765 728 711 358 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2914 3933 4179 669
Size: 988 281 976 1019 246 3079
Fragments arranged by size:
3079 1019 988 976 281 246
HindIII A'AGCT_T
Cuts at: 748 3474 748
Size: 2726 3863
HpaI GTT'AAC
Cuts at: 4179 4179
Size: 6589
KasI G'GCGC_C
Cuts at: 2229 2229
Size: 6589
KpnI G_GTAC'C
Cuts at: 3914 3914
Size: 6589
MfeI C'AATT_G
Cuts at: 4188 4188
Size: 6589
MluI A'CGCG_T
Cuts at: 6465 6465
Size: 6589
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2619 5703 5887 1305
Size: 1314 3084 184 2007
Fragments arranged by size:
3084 2007 1314 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2240 2897 10
Size: 54 1425 343 408 657 3702
Fragments arranged by size:
3702 1425 657 408 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2732 2933 2961 4547 4906 5065 518
Size: 648 1566 201 28 1586 359 159 2042
Fragments arranged by size:
2042 1586 1566 648 359 201 159 28
MspA1I CmG'CkG
Cuts at: 1606 2071 2703 3328 3673 3813 4005 4682 5830
Size: 465 632 625 345 140 192 677 1148
Cuts at: 5830 6075 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 632 625 465 345 245
192 140
NarI GG'CG_CC
Cuts at: 2230 2230
Size: 6589
NcoI C'CATG_G
Cuts at: 513 1065 2997 513
Size: 552 1932 4105
Fragments arranged by size:
4105 1932 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6589
NheI G'CTAG_C
Cuts at: 1052 2059 1052
Size: 1007 5582
NotI GC'GGCC_GC
Cuts at: 4036 4036
Size: 6589
NspI r_CATG'y
Cuts at: 1132 1229 3077 3947 5492 1132
Size: 97 1848 870 1545 2229
Fragments arranged by size:
2229 1848 1545 870 97
PasI CC'CwG_GG
Cuts at: 1348 2179 2710 3311 1348
Size: 831 531 601 4626
Fragments arranged by size:
4626 831 601 531
PciI A'CATG_T
Cuts at: 5488 5488
Size: 6589
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2904 1108
Size: 1796 4793
PfoI T'CCnGG_A
Cuts at: 2169 3033 2169
Size: 864 5725
PmeI GTTT'AAAC
Cuts at: 3893 3893
Size: 6589
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4658 4690 6171 6203 4658
Size: 32 1481 32 5044
Fragments arranged by size:
5044 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3277 3277
Size: 6589
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6589
PsiI TTA'TAA
Cuts at: 4159 4159
Size: 6589
PspOMI G'GGCC_C
Cuts at: 3763 3763
Size: 6589
PstI C_TGCA'G
Cuts at: 830 2625 3262 3941 830
Size: 1795 637 679 3478
Fragments arranged by size:
3478 1795 679 637
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6589
PvuII CAG'CTG
Cuts at: 2703 3673 3813 2703
Size: 970 140 5479
Fragments arranged by size:
5479 970 140
SacI G_AGCT'C
Cuts at: 2225 3291 3417 3549 2225
Size: 1066 126 132 5265
Fragments arranged by size:
5265 1066 132 126
SalI G'TCGA_C
Cuts at: 1936 3931 1936
Size: 1995 4594
SbfI CC_TGCA'GG
Cuts at: 3941 3941
Size: 6589
ScaI AGT'ACT
Cuts at: 4837 6549 4837
Size: 1712 4877
SexAI A'CCwGG_T
Cuts at: 3079 3079
Size: 6589
SfcI C'TryA_G
Cuts at: 826 1045 2621 3258 3937 5072 5753 5944 826
Size: 219 1576 637 679 1135 681 191 1471
Fragments arranged by size:
1576 1471 1135 681 679 637 219 191
SfoI GGC'GCC
Cuts at: 2231 2231
Size: 6589
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6589
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6589
SmaI CCC'GGG
Cuts at: 1872 3608 3916 1872
Size: 1736 308 4545
Fragments arranged by size:
4545 1736 308
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2531 3125 3215 3236 3350 3743
Size: 197 926 588 594 90 21 114 393
Cuts at: 3743 4693 5594 5856 820
Size: 950 901 262 1553
Fragments arranged by size:
1553 950 926 901 594 588 393 262
197 114 90 21
SnaBI TAC'GTA
Cuts at: 493 4380 493
Size: 3887 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6589
SphI G_CATG'C
Cuts at: 1132 3077 3947 1132
Size: 1945 870 3774
Fragments arranged by size:
3774 1945 870
SrfI GCCC'GGGC
Cuts at: 3608 3608
Size: 6589
SspI AAT'ATT
Cuts at: 5 52 4513 5
Size: 47 4461 2081
Fragments arranged by size:
4461 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6589
StyI C'CwwG_G
Cuts at: 513 1065 2997 3048 3453 3540 4028 513
Size: 552 1932 51 405 87 488 3074
Fragments arranged by size:
3074 1932 552 488 405 87 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2702 4616 4633 4786 4971 5416 325
Size: 1255 1122 1914 17 153 185 445 1498
Fragments arranged by size:
1914 1498 1255 1122 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4835 6547 96
Size: 274 80 33 51 1443 2858 1712 138
Fragments arranged by size:
2858 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6549 6581 6549
Size: 32 6557
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6589
XbaI T'CTAG_A
Cuts at: 3925 3925
Size: 6589
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 3103 2011
Size: 1092 5497
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6589
XmaI C'CCGG_G
Cuts at: 1870 3606 3914 1870
Size: 1736 308 4545
Fragments arranged by size:
4545 1736 308
XmnI GAAnn'nnTTC
Cuts at: 4718 4718
Size: 6589
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4397 276
Size: 53 83 186 936 2863 2468
Fragments arranged by size:
2863 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
Bpu10I BsiWI BstZ17I EcoNI EcoRV FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI
RsrII SacII SanDI SapI SfiI SwaI