Restriction map for
pFN21AB8396
(Circular) (Six-base) MAPSORT of: pFN21AB8396.seq Check: 9861 from: 1 to: 6097
pFN21AB8396 6097 bp
With 182 enzymes: SgfI *
October 20, 2009 10:57 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3907 278
Size: 53 83 186 936 2371 2468
Fragments arranged by size:
2468 2371 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3440 1937
Size: 1503 4594
Acc65I G'GTAC_C
Cuts at: 3418 3418
Size: 6097
AclI AA'CG_TT
Cuts at: 1563 4224 4597 1563
Size: 2661 373 3063
Fragments arranged by size:
3063 2661 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4160 5544 5812 1632
Size: 2528 1384 268 1917
Fragments arranged by size:
2528 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5900
AflIII A'CryG_T
Cuts at: 1203 2289 4996 5973 1203
Size: 1086 2707 977 1327
Fragments arranged by size:
2707 1327 1086 977
AgeI A'CCGG_T
Cuts at: 4928 4928
Size: 6097
AhdI GACnn_n'nnGTC
Cuts at: 3196 4826 3196
Size: 1630 4467
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6097
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3015 1184
Size: 1831 4266
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2276 2308 2276
Size: 32 6065
AlwNI CAG_nnn'CTG
Cuts at: 3840 5412 3840
Size: 1572 4525
ApaI G_GGCC'C
Cuts at: 2654 2698 2654
Size: 44 6053
ApaLI G'TGCA_C
Cuts at: 4154 5310 4154
Size: 1156 4941
ApoI r'AATT_y
Cuts at: 1493 3406 3632 3784 5893 1493
Size: 1913 226 152 2109 1697
Fragments arranged by size:
2109 1913 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4651 160
Size: 872 3619 1606
Fragments arranged by size:
3619 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6097
AvaI C'yCGr_G
Cuts at: 1870 1943 2060 2137 2227 2452 2665 2698 2933
Size: 73 117 77 90 225 213 33 235
Cuts at: 2933 3422 1870
Size: 489 4545
Fragments arranged by size:
4545 489 235 225 213 117 90 77
73 33
BamHI G'GATC_C
Cuts at: 2566 3427 3794 2566
Size: 861 367 4869
Fragments arranged by size:
4869 861 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2462 3418 4873 618
Size: 325 217 627 675 956 1455 1842
Fragments arranged by size:
1842 1455 956 675 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2226 2541 2654 2698 2944 3119 3416 1391
Size: 835 315 113 44 246 175 297 4072
Fragments arranged by size:
4072 835 315 297 246 175 113 44
BbeI G_GCGC'C
Cuts at: 2466 2466
Size: 6097
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2572 928
Size: 971 673 4453
Fragments arranged by size:
4453 971 673
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4288 4322 1903
Size: 34 50 34 2267 34 3678
Fragments arranged by size:
3678 2267 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3989 5199 3989
Size: 1210 4887
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6097
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2549 2645 2669
Size: 107 122 71 631 605 877 96 24
Cuts at: 2669 2724 4708 136
Size: 55 1984 1525
Fragments arranged by size:
1984 1525 877 631 605 122 107 96
71 55 24
BglII A'GATC_T
Cuts at: 6092 6092
Size: 6097
BlpI GC'TnA_GC
Cuts at: 3514 3514
Size: 6097
Bme1580I G_kGCm'C
Cuts at: 1790 2490 2622 2641 2654 2698 4158 5314 1790
Size: 700 132 19 13 44 1460 1156 2573
Fragments arranged by size:
2573 1460 1156 700 132 44 19 13
BmgBI CAC'GTC
Cuts at: 1318 2022 2839 3074 1318
Size: 704 817 235 4341
Fragments arranged by size:
4341 817 704 235
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2318 2440 3828
Size: 416 53 83 399 24 901 122 1388
Cuts at: 3828 4786 442
Size: 958 1753
Fragments arranged by size:
1753 1388 958 901 416 399 122 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6097
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2086 2233 2086
Size: 147 5950
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2324 2402 3191 3287 4757 1239
Size: 1085 78 789 96 1470 2579
Fragments arranged by size:
2579 1470 1085 789 96 78
Bpu10I CC'TnA_GC
Cuts at: 2783 3215 2783
Size: 432 5665
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2273 3065 3133 4222 5087 5385 2273
Size: 792 68 1089 865 298 2985
Fragments arranged by size:
2985 1089 865 792 298 68
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4760 882
Size: 617 3261 2219
Fragments arranged by size:
3261 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3019 3226 3888 493
Size: 711 1815 207 662 2702
Fragments arranged by size:
2702 1815 711 662 207
BsaBI GATnn'nnATC
Cuts at: 6091 6091
Size: 6097
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2463 3065 3904 4286
Size: 53 83 186 936 930 602 839 382
Cuts at: 4286 4921 275
Size: 635 1451
Fragments arranged by size:
1451 936 930 839 635 602 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2768 4530 4928 5202 5349 5760 2768
Size: 1762 398 274 147 411 3105
Fragments arranged by size:
3105 1762 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2276 2306 1178
Size: 30 165 30 873 30 4969
Fragments arranged by size:
4969 873 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2297 2857 3326 1186
Size: 1111 560 469 3957
Fragments arranged by size:
3957 1111 560 469
BseYI C'CCAG_C
Cuts at: 2192 2232 2686 5300 5796 2192
Size: 40 454 2614 496 2493
Fragments arranged by size:
2614 2493 496 454 40
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5524
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2280 2416 2512 2553 2661 2728
Size: 275 186 279 136 96 41 108 67
Cuts at: 2728 3547 4308 5336 1540
Size: 819 761 1028 2301
Fragments arranged by size:
2301 1028 819 761 279 275 186 136
108 96 67 41
BsiHKAI G_wGCw'C
Cuts at: 2214 2541 3318 4158 4243 5314 2214
Size: 327 777 840 85 1071 2997
Fragments arranged by size:
2997 1071 840 777 327 85
BsmI GAATG_Cn'
Cuts at: 3608 3701 3608
Size: 93 6004
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2214 2226 2490 2541 2622 2641 2654
Size: 399 424 12 264 51 81 19 13
Cuts at: 2654 2698 2944 3119 3318 3416 4158 4243 5314
Size: 44 246 175 199 98 742 85 1071
Cuts at: 5314 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 424 399 264 246 199
175 98 85 81 51 44 19 13
12
BspEI T'CCGG_A
Cuts at: 2768 5760 2768
Size: 2992 3105
BspHI T'CATG_A
Cuts at: 3986 5716 3986
Size: 1730 4367
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3452 844
Size: 2608 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2358 3079 3984 1394
Size: 429 535 721 905 3507
Fragments arranged by size:
3507 905 721 535 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4592 4766 65
Size: 1183 3344 174 1396
Fragments arranged by size:
3344 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2432 2459 2507 4741 4928 1700
Size: 110 622 27 48 2234 187 2869
Fragments arranged by size:
2869 2234 622 187 110 48 27
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6097
BssHII G'CGCG_C
Cuts at: 1925 2386 1925
Size: 461 5636
BssSI C'ACGA_G
Cuts at: 4157 5169 4157
Size: 1012 5085
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2043 3364 794
Size: 1249 1321 3527
Fragments arranged by size:
3527 1321 1249
BstBI TT'CG_AA
Cuts at: 4901 4901
Size: 6097
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6097
BstYI r'GATC_y
Cuts at: 1972 2566 3427 3794 4179 4196 5648 5734 5746
Size: 594 861 367 385 17 1452 86 12
Cuts at: 5746 6092 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 861 594 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2197 2416 2574 513
Size: 552 1132 219 158 4036
Fragments arranged by size:
4036 1132 552 219 158
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5358
Fragments arranged by size:
5358 683 56
BtsI GCAGTG_nn'
Cuts at: 3613 4407 4427 3613
Size: 794 20 5283
Fragments arranged by size:
5283 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6097
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6062
DraI TTT'AAA
Cuts at: 3401 3401
Size: 6097
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5104 809
Size: 507 3788 1802
Fragments arranged by size:
3788 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2277 2413 2509
Size: 54 1425 50 275 18 447 136 96
Cuts at: 2509 2550 2631 2725 3544 4433 6062 8
Size: 41 81 94 819 889 1629 43
Fragments arranged by size:
1629 1425 889 819 447 275 136 96
94 81 54 50 43 41 18
EagI C'GGCC_G
Cuts at: 1537 1812 2277 2413 2509 2550 2725 3544 1537
Size: 275 465 136 96 41 175 819 4090
Fragments arranged by size:
4090 819 465 275 175 136 96 41
EarI CTCTTCn'nnn_
Cuts at: 1353 2805 2817 4027 1353
Size: 1452 12 1210 3423
Fragments arranged by size:
3423 1452 1210 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2356 4684 5058 5204 387
Size: 1064 138 767 2328 374 146 1280
Fragments arranged by size:
2328 1280 1064 767 374 146 138
EcoICRI GAG'CTC
Cuts at: 2539 2539
Size: 6097
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2324 2402 3191 3287 4160 4757 5544
Size: 393 692 78 789 96 873 597 787
Cuts at: 5544 5812 1239
Size: 268 1524
Fragments arranged by size:
1524 873 789 787 692 597 393 268
96 78
EcoO109I rG'GnC_Cy
Cuts at: 2651 2694 2695 2755 2930 2651
Size: 43 1 60 175 5818
Fragments arranged by size:
5818 175 60 43 1
EcoRI G'AATT_C
Cuts at: 3406 3784 3406
Size: 378 5719
FspI TGC'GCA
Cuts at: 4603 4603
Size: 6097
HaeII r_GCGC'y
Cuts at: 1678 2466 2615 3178 3184 5244 1678
Size: 788 149 563 6 2060 2531
Fragments arranged by size:
2531 2060 788 563 149 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3182 3214 4738 4770 4812
Size: 32 135 32 1610 32 1524 32 42
Cuts at: 4812 4844 1373
Size: 32 2626
Fragments arranged by size:
2626 1610 1524 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3441 3687 669
Size: 988 281 1503 246 3079
Fragments arranged by size:
3079 1503 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6097
HpaI GTT'AAC
Cuts at: 3687 3687
Size: 6097
KasI G'GCGC_C
Cuts at: 2462 2462
Size: 6097
KpnI G_GTAC'C
Cuts at: 3422 3422
Size: 6097
MfeI C'AATT_G
Cuts at: 3696 3696
Size: 6097
MluI A'CGCG_T
Cuts at: 2289 5973 2289
Size: 3684 2413
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2895 5211 5395 1305
Size: 1590 2316 184 2007
Fragments arranged by size:
2316 2007 1590 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4275
Fragments arranged by size:
4275 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2243 2903 2997 4055 4414 4573 518
Size: 648 1077 660 94 1058 359 159 2042
Fragments arranged by size:
2042 1077 1058 660 648 359 159 94
MspA1I CmG'CkG
Cuts at: 1606 2185 2418 2739 3513 4190 5338 5583 1606
Size: 579 233 321 774 677 1148 245 2120
Fragments arranged by size:
2120 1148 774 677 579 321 245 233
NaeI GCC'GGC
Cuts at: 2461 2509 2461
Size: 48 6049
NarI GG'CG_CC
Cuts at: 2463 2463
Size: 6097
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5545
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6097
NgoMIV G'CCGG_C
Cuts at: 2459 2507 2459
Size: 48 6049
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6097
NotI GC'GGCC_GC
Cuts at: 2725 3544 2725
Size: 819 5278
NspI r_CATG'y
Cuts at: 1132 1229 2353 3455 5000 1132
Size: 97 1124 1102 1545 2229
Fragments arranged by size:
2229 1545 1124 1102 97
PasI CC'CwG_GG
Cuts at: 1348 2926 1348
Size: 1578 4519
PciI A'CATG_T
Cuts at: 4996 4996
Size: 6097
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6097
PfoI T'CCnGG_A
Cuts at: 2340 3168 2340
Size: 828 5269
PmeI GTTT'AAAC
Cuts at: 3401 3401
Size: 6097
PmlI CAC'GTG
Cuts at: 3226 3226
Size: 6097
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2276 2308 4166 4198 5679 5711 2276
Size: 32 1858 32 1481 32 2662
Fragments arranged by size:
2662 1858 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2930 2930
Size: 6097
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6097
PsiI TTA'TAA
Cuts at: 3667 3667
Size: 6097
PspOMI G'GGCC_C
Cuts at: 2650 2694 2650
Size: 44 6053
PspXI vC'TCGA_Gb
Cuts at: 2060 2060
Size: 6097
PstI C_TGCA'G
Cuts at: 830 2409 3449 830
Size: 1579 1040 3478
Fragments arranged by size:
3478 1579 1040
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6097
SacI G_AGCT'C
Cuts at: 2541 2541
Size: 6097
SacII CC_GC'GG
Cuts at: 2419 2419
Size: 6097
SalI G'TCGA_C
Cuts at: 1936 3439 1936
Size: 1503 4594
SanDI GG'GwC_CC
Cuts at: 2930 2930
Size: 6097
SbfI CC_TGCA'GG
Cuts at: 3449 3449
Size: 6097
ScaI AGT'ACT
Cuts at: 4345 6057 4345
Size: 1712 4385
SfcI C'TryA_G
Cuts at: 826 1045 2075 2405 3445 4580 5261 5452 826
Size: 219 1030 330 1040 1135 681 191 1471
Fragments arranged by size:
1471 1135 1040 1030 681 330 219 191
SfoI GGC'GCC
Cuts at: 2464 2464
Size: 6097
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6097
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6097
SmaI CCC'GGG
Cuts at: 1872 2139 2454 2700 2935 3424 1872
Size: 267 315 246 235 489 4545
Fragments arranged by size:
4545 489 315 267 246 235
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2060 2252 3080 3112 4201 5102
Size: 197 926 117 192 828 32 1089 901
Cuts at: 5102 5364 820
Size: 262 1553
Fragments arranged by size:
1553 1089 926 901 828 262 197 192
117 32
SnaBI TAC'GTA
Cuts at: 493 3888 493
Size: 3395 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6097
SphI G_CATG'C
Cuts at: 1132 3455 1132
Size: 2323 3774
SspI AAT'ATT
Cuts at: 5 52 4021 5
Size: 47 3969 2081
Fragments arranged by size:
3969 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6097
StyI C'CwwG_G
Cuts at: 513 1065 2877 3258 3536 513
Size: 552 1812 381 278 3074
Fragments arranged by size:
3074 1812 552 381 278
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2300 3186 4124 4141 4294 4479 4924
Size: 1255 720 886 938 17 153 185 445
Cuts at: 4924 325
Size: 1498
Fragments arranged by size:
1498 1255 938 886 720 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4343 6055 96
Size: 274 80 33 51 1443 2366 1712 138
Fragments arranged by size:
2366 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2336 2368 2893 2925 6057 6089 2336
Size: 32 525 32 3132 32 2344
Fragments arranged by size:
3132 2344 525 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2840 1316
Size: 1524 4573
XbaI T'CTAG_A
Cuts at: 3433 3433
Size: 6097
XhoI C'TCGA_G
Cuts at: 1943 2060 1943
Size: 117 5980
XmaI C'CCGG_G
Cuts at: 1870 2137 2452 2698 2933 3422 1870
Size: 267 315 246 235 489 4545
Fragments arranged by size:
4545 489 315 267 246 235
XmnI GAAnn'nnTTC
Cuts at: 4226 4226
Size: 6097
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3905 276
Size: 53 83 186 936 2371 2468
Fragments arranged by size:
2468 2371 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI SacI SacII SalI SanDI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbvCI BfrBI BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI
FseI FspAI NruI NsiI PacI PsrI PvuII RsrII
SapI SexAI SfiI SrfI SwaI XcmI