(Circular) (Six-base) MAPSORT of: pFN21AB8384.seq Check: 5786 from: 1 to: 6553 pFN21AB8384 6553 bp With 182 enzymes: SgfI * February 27, 2009 14:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4363 278 Size: 53 83 186 936 2827 2468 Fragments arranged by size: 2827 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3759 3896 1937 Size: 1822 137 4594 Fragments arranged by size: 4594 1822 137 Acc65I G'GTAC_C Cuts at: 2111 3874 2111 Size: 1763 4790 AclI AA'CG_TT Cuts at: 1563 4680 5053 1563 Size: 3117 373 3063 Fragments arranged by size: 3117 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2081 2186 2647 2781 3057 3327 4616 6000 Size: 449 105 461 134 276 270 1289 1384 Cuts at: 6000 6268 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1289 461 449 276 270 268 134 105 AflII C'TTAA_G Cuts at: 820 1017 2741 820 Size: 197 1724 4632 Fragments arranged by size: 4632 1724 197 AflIII A'CryG_T Cuts at: 1203 2271 5452 6429 1203 Size: 1068 3181 977 1327 Fragments arranged by size: 3181 1327 1068 977 AgeI A'CCGG_T Cuts at: 5384 5384 Size: 6553 AhdI GACnn_n'nnGTC Cuts at: 5282 5282 Size: 6553 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6553 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2988 1184 Size: 1804 4749 AlwNI CAG_nnn'CTG Cuts at: 3134 3467 4296 5868 3134 Size: 333 829 1572 3819 Fragments arranged by size: 3819 1572 829 333 ApaI G_GGCC'C Cuts at: 2056 2573 3013 3641 2056 Size: 517 440 628 4968 Fragments arranged by size: 4968 628 517 440 ApaLI G'TGCA_C Cuts at: 4610 5766 4610 Size: 1156 5397 ApoI r'AATT_y Cuts at: 1493 2168 3442 3675 3862 4088 4240 6349 1493 Size: 675 1274 233 187 226 152 2109 1697 Fragments arranged by size: 2109 1697 1274 675 233 226 187 152 AseI AT'TA_AT Cuts at: 160 1032 5107 160 Size: 872 4075 1606 Fragments arranged by size: 4075 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6553 AvaI C'yCGr_G Cuts at: 1870 1943 2592 2781 3006 3231 3878 1870 Size: 73 649 189 225 225 647 4545 Fragments arranged by size: 4545 649 647 225 225 189 73 BamHI G'GATC_C Cuts at: 2148 2289 2646 3883 4250 2148 Size: 141 357 1237 367 4451 Fragments arranged by size: 4451 1237 367 357 141 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2111 3077 3121 3405 3874 Size: 325 217 627 324 966 44 284 469 Cuts at: 3874 5329 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 966 627 469 325 324 284 217 44 BanII G_rGCy'C Cuts at: 1391 2036 2056 2573 2836 2942 3013 3208 3518 Size: 645 20 517 263 106 71 195 310 Cuts at: 3518 3641 3872 1391 Size: 123 231 4072 Fragments arranged by size: 4072 645 517 310 263 231 195 123 106 71 20 BbeI G_GCGC'C Cuts at: 3409 3409 Size: 6553 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2510 928 Size: 971 611 4971 Fragments arranged by size: 4971 971 611 BbvCI CC'TCA_GC Cuts at: 2070 2070 Size: 6553 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4744 4778 1903 Size: 34 2807 34 3678 Fragments arranged by size: 3678 2807 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3367 4445 5655 3367 Size: 1078 1210 4265 Fragments arranged by size: 4265 1210 1078 BclI T'GATC_A Cuts at: 1548 1548 Size: 6553 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2981 5164 136 Size: 107 122 71 631 605 1309 2183 1525 Fragments arranged by size: 2183 1525 1309 631 605 122 107 71 BglII A'GATC_T Cuts at: 2427 6548 2427 Size: 4121 2432 BlpI GC'TnA_GC Cuts at: 3970 3970 Size: 6553 Bme1580I G_kGCm'C Cuts at: 1790 2056 2573 2623 3013 3082 3124 3141 3239 Size: 266 517 50 390 69 42 17 98 Cuts at: 3239 3641 4614 5770 1790 Size: 402 973 1156 2573 Fragments arranged by size: 2573 1156 973 517 402 390 266 98 69 50 42 17 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6553 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2052 2644 2898 Size: 416 53 83 399 24 635 592 254 Cuts at: 2898 3032 3478 3845 4284 5242 442 Size: 134 446 367 439 958 1753 Fragments arranged by size: 1753 958 635 592 446 439 416 399 367 254 134 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6553 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2247 3561 5213 1239 Size: 1008 1314 1652 2579 Fragments arranged by size: 2579 1652 1314 1008 Bpu10I CC'TnA_GC Cuts at: 2070 3517 2070 Size: 1447 5106 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3440 3475 4678 5543 5841 3440 Size: 35 1203 865 298 4152 Fragments arranged by size: 4152 1203 865 298 35 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5216 882 Size: 617 3717 2219 Fragments arranged by size: 3717 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2909 4344 493 Size: 711 1705 1435 2702 Fragments arranged by size: 2702 1705 1435 711 BsaBI GATnn'nnATC Cuts at: 2352 6547 2352 Size: 4195 2358 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3406 4360 4742 5377 Size: 53 83 186 936 1873 954 382 635 Cuts at: 5377 275 Size: 1451 Fragments arranged by size: 1873 1451 954 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4986 5384 5658 5805 6216 4986 Size: 398 274 147 411 5323 Fragments arranged by size: 5323 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2258 2288 2600 2630 1178 Size: 30 165 30 855 30 312 30 5101 Fragments arranged by size: 5101 855 312 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2279 1186 Size: 1093 5460 BseYI C'CCAG_C Cuts at: 2075 2584 2859 3127 3648 5756 6252 2075 Size: 509 275 268 521 2108 496 2376 Fragments arranged by size: 2376 2108 521 509 496 275 268 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2150 2709 3750 1163 Size: 573 414 559 1041 3966 Fragments arranged by size: 3966 1041 573 559 414 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4003 4764 5792 1540 Size: 275 186 2002 761 1028 2301 Fragments arranged by size: 2301 2002 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2313 2942 3208 4614 4699 5770 2313 Size: 629 266 1406 85 1071 3096 Fragments arranged by size: 3096 1406 1071 629 266 85 BsmI GAATG_Cn' Cuts at: 4064 4157 4064 Size: 93 6460 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2036 2056 2313 2573 2623 2836 2942 Size: 399 246 20 257 260 50 213 106 Cuts at: 2942 3013 3082 3124 3141 3208 3239 3518 3641 Size: 71 69 42 17 67 31 279 123 Cuts at: 3641 3872 4614 4699 5770 1391 Size: 231 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 279 260 257 246 231 213 123 106 85 71 69 67 50 42 31 20 17 BspEI T'CCGG_A Cuts at: 6216 6216 Size: 6553 BspHI T'CATG_A Cuts at: 4442 6172 4442 Size: 1730 4823 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2956 2970 3908 844 Size: 2112 14 938 3489 Fragments arranged by size: 3489 2112 938 14 BsrBI CCG'CTC Cuts at: 1394 1823 2644 2917 4440 1394 Size: 429 821 273 1523 3507 Fragments arranged by size: 3507 1523 821 429 273 BsrDI GCAATG_nn' Cuts at: 65 1248 2266 5048 5222 65 Size: 1183 1018 2782 174 1396 Fragments arranged by size: 2782 1396 1183 1018 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2894 5197 5384 1700 Size: 110 1084 2303 187 2869 Fragments arranged by size: 2869 2303 1084 187 110 BsrGI T'GTAC_A Cuts at: 96 2161 96 Size: 2065 4488 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6553 BssSI C'ACGA_G Cuts at: 4613 5625 4613 Size: 1012 5541 BstAPI GCAn_nnn'nTGC Cuts at: 794 2618 3386 3593 794 Size: 1824 768 207 3754 Fragments arranged by size: 3754 1824 768 207 BstBI TT'CG_AA Cuts at: 5357 5357 Size: 6553 BstEII G'GTnAC_C Cuts at: 1184 3266 3495 1184 Size: 2082 229 4242 Fragments arranged by size: 4242 2082 229 BstYI r'GATC_y Cuts at: 1972 2148 2289 2427 2646 2872 3883 4250 4635 Size: 176 141 138 219 226 1011 367 385 Cuts at: 4635 4652 6104 6190 6202 6548 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1011 385 367 346 226 219 176 141 138 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2212 2868 2212 Size: 656 5897 BtgI C'CryG_G Cuts at: 513 1065 2004 2748 3471 3500 513 Size: 552 939 744 723 29 3566 Fragments arranged by size: 3566 939 744 723 552 29 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5814 Fragments arranged by size: 5814 683 56 BtsI GCAGTG_nn' Cuts at: 3095 3762 4069 4863 4883 3095 Size: 667 307 794 20 4765 Fragments arranged by size: 4765 794 667 307 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6553 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6518 DraI TTT'AAA Cuts at: 3857 3857 Size: 6553 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2512 3290 5560 809 Size: 507 1196 778 2270 1802 Fragments arranged by size: 2270 1802 1196 778 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2461 4000 4889 Size: 54 1425 50 275 18 631 1539 889 Cuts at: 4889 6518 8 Size: 1629 43 Fragments arranged by size: 1629 1539 1425 889 631 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4000 1537 Size: 275 2188 4090 Fragments arranged by size: 4090 2188 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2301 2757 2918 4483 1353 Size: 948 456 161 1565 3423 Fragments arranged by size: 3423 1565 948 456 161 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3018 5140 5514 5660 387 Size: 1064 138 1429 2122 374 146 1280 Fragments arranged by size: 2122 1429 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2940 3206 2940 Size: 266 6287 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2081 2186 2247 2647 2781 3057 3327 Size: 393 449 105 61 400 134 276 270 Cuts at: 3327 3561 4616 5213 6000 6268 1239 Size: 234 1055 597 787 268 1524 Fragments arranged by size: 1524 1055 787 597 449 400 393 276 270 268 234 134 105 61 EcoO109I rG'GnC_Cy Cuts at: 2052 2053 2216 3010 3637 3638 2052 Size: 1 163 794 627 1 4967 Fragments arranged by size: 4967 794 627 163 1 1 EcoRI G'AATT_C Cuts at: 3442 3862 4240 3442 Size: 420 378 5755 Fragments arranged by size: 5755 420 378 FspI TGC'GCA Cuts at: 5059 5059 Size: 6553 HaeII r_GCGC'y Cuts at: 1678 3409 5700 1678 Size: 1731 2291 2531 Fragments arranged by size: 2531 2291 1731 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2222 2254 5194 5226 5268 Size: 32 135 32 650 32 2940 32 42 Cuts at: 5268 5300 1373 Size: 32 2626 Fragments arranged by size: 2940 2626 650 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3897 4143 669 Size: 988 281 1959 246 3079 Fragments arranged by size: 3079 1959 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6553 HpaI GTT'AAC Cuts at: 4143 4143 Size: 6553 KasI G'GCGC_C Cuts at: 3405 3405 Size: 6553 KpnI G_GTAC'C Cuts at: 2115 3878 2115 Size: 1763 4790 MfeI C'AATT_G Cuts at: 4152 4152 Size: 6553 MluI A'CGCG_T Cuts at: 6429 6429 Size: 6553 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5667 5851 1305 Size: 4362 184 2007 Fragments arranged by size: 4362 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4731 Fragments arranged by size: 4731 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2733 3149 4511 4870 5029 518 Size: 648 1567 416 1362 359 159 2042 Fragments arranged by size: 2042 1567 1362 648 416 359 159 MspA1I CmG'CkG Cuts at: 1606 3134 3305 3467 3539 3969 4646 5794 6039 Size: 1528 171 162 72 430 677 1148 245 Cuts at: 6039 1606 Size: 2120 Fragments arranged by size: 2120 1528 1148 677 430 245 171 162 72 NarI GG'CG_CC Cuts at: 3406 3406 Size: 6553 NcoI C'CATG_G Cuts at: 513 1065 2004 2748 3471 3500 513 Size: 552 939 744 723 29 3566 Fragments arranged by size: 3566 939 744 723 552 29 NdeI CA'TA_TG Cuts at: 387 387 Size: 6553 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6553 NotI GC'GGCC_GC Cuts at: 4000 4000 Size: 6553 NspI r_CATG'y Cuts at: 1132 1229 2275 2732 2777 3800 3911 5456 1132 Size: 97 1046 457 45 1023 111 1545 2229 Fragments arranged by size: 2229 1545 1046 1023 457 111 97 45 PasI CC'CwG_GG Cuts at: 1348 3598 1348 Size: 2250 4303 PciI A'CATG_T Cuts at: 2271 5452 2271 Size: 3181 3372 PflMI CCAn_nnn'nTGG Cuts at: 1108 2807 2828 1108 Size: 1699 21 4833 Fragments arranged by size: 4833 1699 21 PmeI GTTT'AAAC Cuts at: 3857 3857 Size: 6553 PmlI CAC'GTG Cuts at: 2909 2909 Size: 6553 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4622 4654 6135 6167 4622 Size: 32 1481 32 5008 Fragments arranged by size: 5008 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2216 2216 Size: 6553 PshAI GACnn'nnGTC Cuts at: 1613 2322 1613 Size: 709 5844 PsiI TTA'TAA Cuts at: 4123 4123 Size: 6553 PspOMI G'GGCC_C Cuts at: 2052 2569 3009 3637 2052 Size: 517 440 628 4968 Fragments arranged by size: 4968 628 517 440 PstI C_TGCA'G Cuts at: 830 2967 3905 830 Size: 2137 938 3478 Fragments arranged by size: 3478 2137 938 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6553 PvuII CAG'CTG Cuts at: 3134 3305 3467 3539 3134 Size: 171 162 72 6148 Fragments arranged by size: 6148 171 162 72 SacI G_AGCT'C Cuts at: 2942 3208 2942 Size: 266 6287 SalI G'TCGA_C Cuts at: 1936 3895 1936 Size: 1959 4594 SapI GCTCTTCn'nnn_ Cuts at: 2301 2301 Size: 6553 SbfI CC_TGCA'GG Cuts at: 2967 3905 2967 Size: 938 5615 ScaI AGT'ACT Cuts at: 2363 3591 4801 6513 2363 Size: 1228 1210 1712 2403 Fragments arranged by size: 2403 1712 1228 1210 SfcI C'TryA_G Cuts at: 826 1045 2281 2485 2696 2963 3901 5036 5717 Size: 219 1236 204 211 267 938 1135 681 Cuts at: 5717 5908 826 Size: 191 1471 Fragments arranged by size: 1471 1236 1135 938 681 267 219 211 204 191 SfoI GGC'GCC Cuts at: 3407 3407 Size: 6553 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6553 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6553 SmaI CCC'GGG Cuts at: 1872 3008 3233 3880 1872 Size: 1136 225 647 4545 Fragments arranged by size: 4545 1136 647 225 SmlI C'TyrA_G Cuts at: 820 1017 1943 2592 2741 3455 3490 4657 5558 Size: 197 926 649 149 714 35 1167 901 Cuts at: 5558 5820 820 Size: 262 1553 Fragments arranged by size: 1553 1167 926 901 714 649 262 197 149 35 SnaBI TAC'GTA Cuts at: 493 4344 493 Size: 3851 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6553 SphI G_CATG'C Cuts at: 1132 3911 1132 Size: 2779 3774 SspI AAT'ATT Cuts at: 5 52 2480 4477 5 Size: 47 2428 1997 2081 Fragments arranged by size: 2428 2081 1997 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6553 StyI C'CwwG_G Cuts at: 513 1065 2004 2121 2508 2748 3271 3471 3500 Size: 552 939 117 387 240 523 200 29 Cuts at: 3500 3681 3992 513 Size: 181 311 3074 Fragments arranged by size: 3074 939 552 523 387 311 240 200 181 117 29 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4580 4597 4750 4935 5380 325 Size: 1255 3000 17 153 185 445 1498 Fragments arranged by size: 3000 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2161 2249 2268 Size: 274 80 33 51 1443 184 88 19 Cuts at: 2268 2361 3589 4799 6511 96 Size: 93 1228 1210 1712 138 Fragments arranged by size: 1712 1443 1228 1210 274 184 138 93 88 80 51 33 19 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2350 2382 2600 2632 2911 2943 6513 6545 2350 Size: 32 218 32 279 32 3570 32 2358 Fragments arranged by size: 3570 2358 279 218 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6553 XbaI T'CTAG_A Cuts at: 3889 3889 Size: 6553 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3507 3507 Size: 6553 XhoI C'TCGA_G Cuts at: 1943 2592 1943 Size: 649 5904 XmaI C'CCGG_G Cuts at: 1870 3006 3231 3878 1870 Size: 1136 225 647 4545 Fragments arranged by size: 4545 1136 647 225 XmnI GAAnn'nnTTC Cuts at: 4682 4682 Size: 6553 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4361 276 Size: 53 83 186 936 2827 2468 Fragments arranged by size: 2827 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI