Restriction map for
pFN21AB8377
(Circular) (Six-base) MAPSORT of: pFN21AB8377.seq Check: 6354 from: 1 to: 6571
pFN21AB8377 6571 bp
With 182 enzymes: SgfI *
February 27, 2009 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2234 3051 3381 3426 3672 2234
Size: 817 330 45 246 5133
Fragments arranged by size:
5133 817 330 246 45
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4381 278
Size: 53 83 186 936 2845 2468
Fragments arranged by size:
2845 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3914 1937
Size: 1977 4594
Acc65I G'GTAC_C
Cuts at: 3892 3892
Size: 6571
AclI AA'CG_TT
Cuts at: 1563 4698 5071 1563
Size: 3135 373 3063
Fragments arranged by size:
3135 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3362 3653 3698 4634 6018 6286 1632
Size: 1730 291 45 936 1384 268 1917
Fragments arranged by size:
1917 1730 1384 936 291 268 45
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6374
AflIII A'CryG_T
Cuts at: 1203 5470 6447 1203
Size: 4267 977 1327
Fragments arranged by size:
4267 1327 977
AgeI A'CCGG_T
Cuts at: 5402 5402
Size: 6571
AhdI GACnn_n'nnGTC
Cuts at: 5300 5300
Size: 6571
AleI CACnn'nnGTG
Cuts at: 1166 2024 3715 1166
Size: 858 1691 4022
Fragments arranged by size:
4022 1691 858
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3079 1184
Size: 1895 4676
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3131 3163 3131
Size: 32 6539
AlwNI CAG_nnn'CTG
Cuts at: 2662 2881 3676 4314 5886 2662
Size: 219 795 638 1572 3347
Fragments arranged by size:
3347 1572 795 638 219
ApaI G_GGCC'C
Cuts at: 2340 3177 2340
Size: 837 5734
ApaLI G'TGCA_C
Cuts at: 4628 5784 4628
Size: 1156 5415
ApoI r'AATT_y
Cuts at: 1493 3428 3836 3880 4106 4258 6367 1493
Size: 1935 408 44 226 152 2109 1697
Fragments arranged by size:
2109 1935 1697 408 226 152 44
AseI AT'TA_AT
Cuts at: 160 1032 5125 160
Size: 872 4093 1606
Fragments arranged by size:
4093 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6571
AvaI C'yCGr_G
Cuts at: 1870 1943 3443 3896 1870
Size: 73 1500 453 4545
Fragments arranged by size:
4545 1500 453 73
BamHI G'GATC_C
Cuts at: 3801 3901 4268 3801
Size: 100 367 6104
Fragments arranged by size:
6104 367 100
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3063 3404 3438 3892 5347
Size: 325 217 627 1276 341 34 454 1455
Cuts at: 5347 618
Size: 1842
Fragments arranged by size:
1842 1455 1276 627 454 341 325 217
34
BanII G_rGCy'C
Cuts at: 1391 2033 2154 2163 2184 2340 2358 3177 3235
Size: 642 121 9 21 156 18 819 58
Cuts at: 3235 3890 1391
Size: 655 4072
Fragments arranged by size:
4072 819 655 642 156 121 58 21
18 9
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5600
BbvCI CC'TCA_GC
Cuts at: 2568 2568
Size: 6571
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3424 3458 3521 3555 4762 4796 1903
Size: 34 1487 34 63 34 1207 34 3678
Fragments arranged by size:
3678 1487 1207 63 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3586 4463 5673 3586
Size: 877 1210 4484
Fragments arranged by size:
4484 1210 877
BclI T'GATC_A
Cuts at: 1548 2664 1548
Size: 1116 5455
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2090 2433 5182
Size: 107 122 71 631 605 418 343 2749
Cuts at: 5182 136
Size: 1525
Fragments arranged by size:
2749 1525 631 605 418 343 122 107
71
BglII A'GATC_T
Cuts at: 6566 6566
Size: 6571
BlpI GC'TnA_GC
Cuts at: 3266 3988 3266
Size: 722 5849
Bme1580I G_kGCm'C
Cuts at: 1790 2340 3177 3409 3638 4632 5788 1790
Size: 550 837 232 229 994 1156 2573
Fragments arranged by size:
2573 1156 994 837 550 232 229
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6571
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2768 2891 3410
Size: 416 53 83 399 24 1351 123 519
Cuts at: 3410 4302 5260 442
Size: 892 958 1753
Fragments arranged by size:
1753 1351 958 892 519 416 399 123
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6571
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2180 2180
Size: 6571
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2017 2336 2639 2876 2924 2933 3153 3746
Size: 778 319 303 237 48 9 220 593
Cuts at: 3746 5231 1239
Size: 1485 2579
Fragments arranged by size:
2579 1485 778 593 319 303 237 220
48 9
Bpu10I CC'TnA_GC
Cuts at: 2133 2357 2568 2133
Size: 224 211 6136
Fragments arranged by size:
6136 224 211
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4696 5561 5859 4696
Size: 865 298 5408
Fragments arranged by size:
5408 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2489 2657 3364 3771 5234 882
Size: 617 990 168 707 407 1463 2219
Fragments arranged by size:
2219 1463 990 707 617 407 168
BsaAI yAC'GTr
Cuts at: 493 1204 4362 493
Size: 711 3158 2702
Fragments arranged by size:
3158 2702 711
BsaBI GATnn'nnATC
Cuts at: 6565 6565
Size: 6571
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3822 4378 4760 5395
Size: 53 83 186 936 2289 556 382 635
Cuts at: 5395 275
Size: 1451
Fragments arranged by size:
2289 1451 936 635 556 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2102 5004 5402 5676 5823 6234 2102
Size: 2902 398 274 147 411 2439
Fragments arranged by size:
2902 2439 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2396 2426 3131 3161 1178
Size: 30 165 30 993 30 705 30 4588
Fragments arranged by size:
4588 993 705 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2153 3272 3608 1186
Size: 967 1119 336 4149
Fragments arranged by size:
4149 1119 967 336
BseYI C'CCAG_C
Cuts at: 2563 5774 6270 2563
Size: 3211 496 2864
Fragments arranged by size:
3211 2864 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2405 3647 3693 1163
Size: 573 669 1242 46 4041
Fragments arranged by size:
4041 1242 669 573 46
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4021 4782 5810 1540
Size: 275 186 2020 761 1028 2301
Fragments arranged by size:
2301 2020 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2800 4632 4717 5788 2800
Size: 1832 85 1071 3583
Fragments arranged by size:
3583 1832 1071 85
BsmI GAATG_Cn'
Cuts at: 4082 4175 4082
Size: 93 6478
BsmBI CGTCTCn'nnnn_
Cuts at: 3081 3081
Size: 6571
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2033 2154 2163 2184 2340 2358 2800
Size: 399 243 121 9 21 156 18 442
Cuts at: 2800 3177 3235 3409 3638 3890 4632 4717 5788
Size: 377 58 174 229 252 742 85 1071
Cuts at: 5788 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 442 399 377 252 243
229 174 156 121 85 58 21 18
9
BspEI T'CCGG_A
Cuts at: 6234 6234
Size: 6571
BspHI T'CATG_A
Cuts at: 2701 4460 6190 2701
Size: 1759 1730 3082
Fragments arranged by size:
3082 1759 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2234 3051 3120 3381 3426 3672 3926 844
Size: 1390 817 69 261 45 246 254 3489
Fragments arranged by size:
3489 1390 817 261 254 246 69 45
BsrBI CCG'CTC
Cuts at: 1394 1823 4458 1394
Size: 429 2635 3507
Fragments arranged by size:
3507 2635 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5066 5240 65
Size: 1183 3818 174 1396
Fragments arranged by size:
3818 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2067 5215 5402 1700
Size: 110 257 3148 187 2869
Fragments arranged by size:
3148 2869 257 187 110
BsrGI T'GTAC_A
Cuts at: 96 2748 96
Size: 2652 3919
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6571
BssSI C'ACGA_G
Cuts at: 4631 5643 4631
Size: 1012 5559
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6571
BstBI TT'CG_AA
Cuts at: 5375 5375
Size: 6571
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6571
BstXI CCAn_nnnn'nTGG
Cuts at: 2026 2041 2026
Size: 15 6556
BstYI r'GATC_y
Cuts at: 1972 2530 3801 3901 4268 4653 4670 6122 6208
Size: 558 1271 100 367 385 17 1452 86
Cuts at: 6208 6220 6566 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1271 558 385 367 346 100
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2243 2713 3186 3468 2243
Size: 470 473 282 5346
Fragments arranged by size:
5346 473 470 282
BtgI C'CryG_G
Cuts at: 513 1065 2726 513
Size: 552 1661 4358
Fragments arranged by size:
4358 1661 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5832
Fragments arranged by size:
5832 683 56
BtsI GCAGTG_nn'
Cuts at: 3527 3705 4087 4881 4901 3527
Size: 178 382 794 20 5197
Fragments arranged by size:
5197 794 382 178 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6571
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6536
DraI TTT'AAA
Cuts at: 3875 3875
Size: 6571
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5578 809
Size: 507 4262 1802
Fragments arranged by size:
4262 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3586 4018 4907
Size: 54 1425 50 275 18 1756 432 889
Cuts at: 4907 6536 8
Size: 1629 43
Fragments arranged by size:
1756 1629 1425 889 432 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4018 1537
Size: 275 2206 4090
Fragments arranged by size:
4090 2206 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3458 3674 4501 1353
Size: 2105 216 827 3423
Fragments arranged by size:
3423 2105 827 216
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5158 5532 5678 387
Size: 1064 138 3569 374 146 1280
Fragments arranged by size:
3569 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2017 2336 2639 2876 2924 2933 3153
Size: 393 385 319 303 237 48 9 220
Cuts at: 3153 3362 3653 3698 3746 4634 5231 6018 6286
Size: 209 291 45 48 888 597 787 268
Cuts at: 6286 1239
Size: 1524
Fragments arranged by size:
1524 888 787 597 393 385 319 303
291 268 237 220 209 48 48 45
9
EcoNI CCTnn'n_nnAGG
Cuts at: 2825 3377 2825
Size: 552 6019
EcoO109I rG'GnC_Cy
Cuts at: 2336 2476 2763 2843 3173 3174 2336
Size: 140 287 80 330 1 5733
Fragments arranged by size:
5733 330 287 140 80 1
EcoRI G'AATT_C
Cuts at: 3880 4258 3880
Size: 378 6193
EcoRV GAT'ATC
Cuts at: 2290 2290
Size: 6571
FspI TGC'GCA
Cuts at: 5077 5077
Size: 6571
HaeII r_GCGC'y
Cuts at: 1678 5718 1678
Size: 4040 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2396 2428 2770 2802 2993
Size: 32 135 32 824 32 342 32 191
Cuts at: 2993 3025 3673 3705 5212 5244 5286 5318 1373
Size: 32 648 32 1507 32 42 32 2626
Fragments arranged by size:
2626 1507 824 648 342 191 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3915 4161 669
Size: 988 281 1977 246 3079
Fragments arranged by size:
3079 1977 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2251 748
Size: 1503 5068
HpaI GTT'AAC
Cuts at: 4161 4161
Size: 6571
KpnI G_GTAC'C
Cuts at: 3896 3896
Size: 6571
MfeI C'AATT_G
Cuts at: 4170 4170
Size: 6571
MluI A'CGCG_T
Cuts at: 6447 6447
Size: 6571
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2325 5685 5869 1305
Size: 1020 3360 184 2007
Fragments arranged by size:
3360 2007 1020 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4749
Fragments arranged by size:
4749 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2024 3715 3753 4529 4888 5047 518
Size: 648 858 1691 38 776 359 159 2042
Fragments arranged by size:
2042 1691 858 776 648 359 159 38
MspA1I CmG'CkG
Cuts at: 1606 2037 2192 2296 2567 3206 3585 3987 4664
Size: 431 155 104 271 639 379 402 677
Cuts at: 4664 5812 6057 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 639 431 402 379 271
245 155 104
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6019
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6571
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6571
NotI GC'GGCC_GC
Cuts at: 4018 4018
Size: 6571
NspI r_CATG'y
Cuts at: 1132 1229 3929 5474 1132
Size: 97 2700 1545 2229
Fragments arranged by size:
2700 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2260 2605 1348
Size: 912 345 5314
Fragments arranged by size:
5314 912 345
PciI A'CATG_T
Cuts at: 5470 5470
Size: 6571
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3415 1108
Size: 2307 4264
PfoI T'CCnGG_A
Cuts at: 2655 2655
Size: 6571
PmeI GTTT'AAAC
Cuts at: 3875 3875
Size: 6571
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2340 2372 3131 3163 4640 4672 6153 6185 2340
Size: 32 759 32 1477 32 1481 32 2726
Fragments arranged by size:
2726 1481 1477 759 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2476 2763 2843 2476
Size: 287 80 6204
Fragments arranged by size:
6204 287 80
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6571
PsiI TTA'TAA
Cuts at: 4141 4141
Size: 6571
PspOMI G'GGCC_C
Cuts at: 2336 3173 2336
Size: 837 5734
PspXI vC'TCGA_Gb
Cuts at: 3443 3443
Size: 6571
PstI C_TGCA'G
Cuts at: 830 2191 2442 2598 3097 3534 3923 830
Size: 1361 251 156 499 437 389 3478
Fragments arranged by size:
3478 1361 499 437 389 251 156
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6571
PvuII CAG'CTG
Cuts at: 2037 2192 2567 2037
Size: 155 375 6041
Fragments arranged by size:
6041 375 155
SalI G'TCGA_C
Cuts at: 1936 3913 1936
Size: 1977 4594
SapI GCTCTTCn'nnn_
Cuts at: 3458 3458
Size: 6571
SbfI CC_TGCA'GG
Cuts at: 2442 2598 3923 2442
Size: 156 1325 5090
Fragments arranged by size:
5090 1325 156
ScaI AGT'ACT
Cuts at: 4819 6531 4819
Size: 1712 4859
SexAI A'CCwGG_T
Cuts at: 3867 3867
Size: 6571
SfcI C'TryA_G
Cuts at: 826 1045 2187 2438 2594 3068 3093 3530 3919
Size: 219 1142 251 156 474 25 437 389
Cuts at: 3919 5054 5735 5926 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1142 1135 681 474 437 389 251
219 191 156 25
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6571
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6571
SmaI CCC'GGG
Cuts at: 1872 3898 1872
Size: 2026 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3443 4675 5576 5838 820
Size: 197 926 1500 1232 901 262 1553
Fragments arranged by size:
1553 1500 1232 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4362 493
Size: 3869 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6571
SphI G_CATG'C
Cuts at: 1132 3929 1132
Size: 2797 3774
SspI AAT'ATT
Cuts at: 5 52 4495 5
Size: 47 4443 2081
Fragments arranged by size:
4443 2081 47
StuI AGG'CCT
Cuts at: 1517 2718 2861 2930 3389 1517
Size: 1201 143 69 459 4699
Fragments arranged by size:
4699 1201 459 143 69
StyI C'CwwG_G
Cuts at: 513 1065 2085 2208 2647 3143 3330 4010 513
Size: 552 1020 123 439 496 187 680 3074
Fragments arranged by size:
3074 1020 680 552 496 439 187 123
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3383 3392 4598 4615 4768 4953 5398
Size: 1255 1803 9 1206 17 153 185 445
Cuts at: 5398 325
Size: 1498
Fragments arranged by size:
1803 1498 1255 1206 445 185 153 17
9
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2748 2805 4817
Size: 274 80 33 51 1443 771 57 2012
Cuts at: 4817 6529 96
Size: 1712 138
Fragments arranged by size:
2012 1712 1443 771 274 138 80 57
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6531 6563 6531
Size: 32 6539
Tth111I GACn'n_nGTC
Cuts at: 1316 2375 1316
Size: 1059 5512
XbaI T'CTAG_A
Cuts at: 3907 3907
Size: 6571
XhoI C'TCGA_G
Cuts at: 1943 3443 1943
Size: 1500 5071
XmaI C'CCGG_G
Cuts at: 1870 3896 1870
Size: 2026 4545
XmnI GAAnn'nnTTC
Cuts at: 3837 4700 3837
Size: 863 5708
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4379 276
Size: 53 83 186 936 2845 2468
Fragments arranged by size:
2845 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SalI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I
DraIII EcoICRI FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PsrI RsrII SacI
SacII SanDI SfiI SfoI SrfI SwaI XcmI