Restriction map for
pFN21AB8374
(Circular) (Six-base) MAPSORT of: pFN21AB8374.seq Check: 3315 from: 1 to: 6589
pFN21AB8374 6589 bp
With 182 enzymes: SgfI *
February 27, 2009 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3309 4399 278
Size: 53 83 186 936 1773 1090 2468
Fragments arranged by size:
2468 1773 1090 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3345 3932 1937
Size: 1408 587 4594
Fragments arranged by size:
4594 1408 587
Acc65I G'GTAC_C
Cuts at: 3910 3910
Size: 6589
AclI AA'CG_TT
Cuts at: 1563 4716 5089 1563
Size: 3153 373 3063
Fragments arranged by size:
3153 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2511 2712 4652 6036 6304 1632
Size: 879 201 1940 1384 268 1917
Fragments arranged by size:
1940 1917 1384 879 268 201
AfeI AGC'GCT
Cuts at: 2054 2054
Size: 6589
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6392
AflIII A'CryG_T
Cuts at: 1203 5488 6465 1203
Size: 4285 977 1327
Fragments arranged by size:
4285 1327 977
AgeI A'CCGG_T
Cuts at: 5420 5420
Size: 6589
AhdI GACnn_n'nnGTC
Cuts at: 5318 5318
Size: 6589
AleI CACnn'nnGTG
Cuts at: 1166 2417 1166
Size: 1251 5338
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6589
AlwNI CAG_nnn'CTG
Cuts at: 3110 4332 5904 3110
Size: 1222 1572 3795
Fragments arranged by size:
3795 1572 1222
ApaI G_GGCC'C
Cuts at: 3446 3715 3446
Size: 269 6320
ApaLI G'TGCA_C
Cuts at: 4646 5802 4646
Size: 1156 5433
ApoI r'AATT_y
Cuts at: 1493 2639 3834 3898 4124 4276 6385 1493
Size: 1146 1195 64 226 152 2109 1697
Fragments arranged by size:
2109 1697 1195 1146 226 152 64
AseI AT'TA_AT
Cuts at: 160 1032 5143 160
Size: 872 4111 1606
Fragments arranged by size:
4111 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6589
AvaI C'yCGr_G
Cuts at: 1870 1943 3037 3914 1870
Size: 73 1094 877 4545
Fragments arranged by size:
4545 1094 877 73
BamHI G'GATC_C
Cuts at: 3919 4286 3919
Size: 367 6222
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3910 5365 618
Size: 325 217 627 2123 1455 1842
Fragments arranged by size:
2123 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2686 3446 3715 3908 1391
Size: 1295 760 269 193 4072
Fragments arranged by size:
4072 1295 760 269 193
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2389 2953 3546 928
Size: 971 490 564 593 3971
Fragments arranged by size:
3971 971 593 564 490
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4780 4814 1903
Size: 34 2843 34 3678
Fragments arranged by size:
3678 2843 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4481 5691 4481
Size: 1210 5379
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6589
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5200 136
Size: 107 122 71 631 605 3528 1525
Fragments arranged by size:
3528 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6584 6584
Size: 6589
BlpI GC'TnA_GC
Cuts at: 2031 2056 4006 2031
Size: 25 1950 4614
Fragments arranged by size:
4614 1950 25
Bme1580I G_kGCm'C
Cuts at: 1790 3446 3715 4650 5806 1790
Size: 1656 269 935 1156 2573
Fragments arranged by size:
2573 1656 1156 935 269
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6589
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2265 2405 4320
Size: 416 53 83 399 24 848 140 1915
Cuts at: 4320 5278 442
Size: 958 1753
Fragments arranged by size:
1915 1753 958 848 416 399 140 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6589
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2336 2781 2828 3066 3226 5249 1239
Size: 1097 445 47 238 160 2023 2579
Fragments arranged by size:
2579 2023 1097 445 238 160 47
Bpu10I CC'TnA_GC
Cuts at: 2853 2853
Size: 6589
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4714 5579 5877 4714
Size: 865 298 5426
Fragments arranged by size:
5426 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2453 5252 882
Size: 617 954 2799 2219
Fragments arranged by size:
2799 2219 954 617
BsaAI yAC'GTr
Cuts at: 493 1204 4380 493
Size: 711 3176 2702
Fragments arranged by size:
3176 2702 711
BsaBI GATnn'nnATC
Cuts at: 2160 6583 2160
Size: 4423 2166
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3306 4396 4778 5413
Size: 53 83 186 936 1773 1090 382 635
Cuts at: 5413 275
Size: 1451
Fragments arranged by size:
1773 1451 1090 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2212 2658 3281 5022 5420 5694 5841 6252 2212
Size: 446 623 1741 398 274 147 411 2549
Fragments arranged by size:
2549 1741 623 446 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2452 2482 3236 3266 1178
Size: 30 165 30 1049 30 754 30 4501
Fragments arranged by size:
4501 1049 754 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2108 2239 2460 3875 1186
Size: 922 131 221 1415 3900
Fragments arranged by size:
3900 1415 922 221 131
BseYI C'CCAG_C
Cuts at: 5792 6288 5792
Size: 496 6093
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3096 3107 1163
Size: 573 1360 11 4645
Fragments arranged by size:
4645 1360 573 11
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2725 4039 4800 5828 1540
Size: 275 186 724 1314 761 1028 2301
Fragments arranged by size:
2301 1314 1028 761 724 275 186
BsiHKAI G_wGCw'C
Cuts at: 2267 2352 4650 4735 5806 2267
Size: 85 2298 85 1071 3050
Fragments arranged by size:
3050 2298 1071 85 85
BsmI GAATG_Cn'
Cuts at: 3163 4100 4193 3163
Size: 937 93 5559
Fragments arranged by size:
5559 937 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3297 3297
Size: 6589
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2267 2352 2686 3446 3715 3908 4650
Size: 399 477 85 334 760 269 193 742
Cuts at: 4650 4735 5806 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 760 742 477 399 334 269
193 85 85
BspEI T'CCGG_A
Cuts at: 6252 6252
Size: 6589
BspHI T'CATG_A
Cuts at: 4478 6208 4478
Size: 1730 4859
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3365 3944 844
Size: 2521 579 3489
Fragments arranged by size:
3489 2521 579
BsrBI CCG'CTC
Cuts at: 1394 1823 3526 4476 1394
Size: 429 1703 950 3507
Fragments arranged by size:
3507 1703 950 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5084 5258 65
Size: 1183 3836 174 1396
Fragments arranged by size:
3836 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5233 5420 1700
Size: 110 3423 187 2869
Fragments arranged by size:
3423 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3252 96
Size: 3156 3433
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6589
BssSI C'ACGA_G
Cuts at: 4649 5661 4649
Size: 1012 5577
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6589
BstBI TT'CG_AA
Cuts at: 5393 5393
Size: 6589
BstEII G'GTnAC_C
Cuts at: 1184 2410 3398 1184
Size: 1226 988 4375
Fragments arranged by size:
4375 1226 988
BstYI r'GATC_y
Cuts at: 1972 2622 3919 4286 4671 4688 6140 6226 6238
Size: 650 1297 367 385 17 1452 86 12
Cuts at: 6238 6584 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1297 650 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6037
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5850
Fragments arranged by size:
5850 683 56
BtsI GCAGTG_nn'
Cuts at: 2333 3334 3444 4105 4899 4919 2333
Size: 1001 110 661 794 20 4003
Fragments arranged by size:
4003 1001 794 661 110 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6589
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6554
DraI TTT'AAA
Cuts at: 3893 3893
Size: 6589
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3491 5596 809
Size: 507 2175 2105 1802
Fragments arranged by size:
2175 2105 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2533 2722 3672
Size: 54 1425 50 275 18 703 189 950
Cuts at: 3672 4036 4925 6554 8
Size: 364 889 1629 43
Fragments arranged by size:
1629 1425 950 889 703 364 275 189
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2722 4036 1537
Size: 275 910 1314 4090
Fragments arranged by size:
4090 1314 910 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2067 2085 2487 4519 1353
Size: 714 18 402 2032 3423
Fragments arranged by size:
3423 2032 714 402 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3679 5176 5550 5696 387
Size: 1064 138 2090 1497 374 146 1280
Fragments arranged by size:
2090 1497 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2336 2511 2712 2781 2828 3066 3226
Size: 393 704 175 201 69 47 238 160
Cuts at: 3226 4652 5249 6036 6304 1239
Size: 1426 597 787 268 1524
Fragments arranged by size:
1524 1426 787 704 597 393 268 238
201 175 160 69 47
EcoO109I rG'GnC_Cy
Cuts at: 2319 3150 3822 2319
Size: 831 672 5086
Fragments arranged by size:
5086 831 672
EcoRI G'AATT_C
Cuts at: 3898 4276 3898
Size: 378 6211
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2071 2071
Size: 6589
FspI TGC'GCA
Cuts at: 5095 5095
Size: 6589
HaeII r_GCGC'y
Cuts at: 1678 2056 3622 5736 1678
Size: 378 1566 2114 2531
Fragments arranged by size:
2531 2114 1566 378
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2452 2484 3206 3238 3671
Size: 32 135 32 880 32 722 32 433
Cuts at: 3671 3703 5230 5262 5304 5336 1373
Size: 32 1527 32 42 32 2626
Fragments arranged by size:
2626 1527 880 722 433 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2601 3933 4179 669
Size: 988 281 663 1332 246 3079
Fragments arranged by size:
3079 1332 988 663 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6589
HpaI GTT'AAC
Cuts at: 4179 4179
Size: 6589
KpnI G_GTAC'C
Cuts at: 3914 3914
Size: 6589
MfeI C'AATT_G
Cuts at: 4188 4188
Size: 6589
MluI A'CGCG_T
Cuts at: 6465 6465
Size: 6589
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2724 3683 3754 5703 5887 1305
Size: 1419 959 71 1949 184 2007
Fragments arranged by size:
2007 1949 1419 959 184 71
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2535 3674 10
Size: 54 1425 343 703 1139 2925
Fragments arranged by size:
2925 1425 1139 703 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2417 2828 3437 3798 4547 4906 5065
Size: 648 1251 411 609 361 749 359 159
Cuts at: 5065 518
Size: 2042
Fragments arranged by size:
2042 1251 749 648 609 411 361 359
159
MspA1I CmG'CkG
Cuts at: 1606 2356 2721 3091 3107 4005 4682 5830 6075
Size: 750 365 370 16 898 677 1148 245
Cuts at: 6075 1606
Size: 2120
Fragments arranged by size:
2120 1148 898 750 677 370 365 245
16
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6037
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6589
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6589
NotI GC'GGCC_GC
Cuts at: 4036 4036
Size: 6589
NspI r_CATG'y
Cuts at: 1132 1229 3220 3947 5492 1132
Size: 97 1991 727 1545 2229
Fragments arranged by size:
2229 1991 1545 727 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6589
PciI A'CATG_T
Cuts at: 5488 5488
Size: 6589
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6589
PfoI T'CCnGG_A
Cuts at: 3203 3203
Size: 6589
PmeI GTTT'AAAC
Cuts at: 3893 3893
Size: 6589
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4658 4690 6171 6203 4658
Size: 32 1481 32 5044
Fragments arranged by size:
5044 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2319 3150 3822 2319
Size: 831 672 5086
Fragments arranged by size:
5086 831 672
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6589
PsiI TTA'TAA
Cuts at: 4159 4159
Size: 6589
PspOMI G'GGCC_C
Cuts at: 3442 3711 3442
Size: 269 6320
PstI C_TGCA'G
Cuts at: 830 2328 2361 3941 830
Size: 1498 33 1580 3478
Fragments arranged by size:
3478 1580 1498 33
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6589
PvuII CAG'CTG
Cuts at: 2356 3107 2356
Size: 751 5838
SalI G'TCGA_C
Cuts at: 1936 3931 1936
Size: 1995 4594
SanDI GG'GwC_CC
Cuts at: 3150 3150
Size: 6589
SbfI CC_TGCA'GG
Cuts at: 3941 3941
Size: 6589
ScaI AGT'ACT
Cuts at: 4837 6549 4837
Size: 1712 4877
SfcI C'TryA_G
Cuts at: 826 1045 2324 2357 2716 3937 5072 5753 5944
Size: 219 1279 33 359 1221 1135 681 191
Cuts at: 5944 826
Size: 1471
Fragments arranged by size:
1471 1279 1221 1135 681 359 219 191
33
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6589
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6589
SmaI CCC'GGG
Cuts at: 1872 3916 1872
Size: 2044 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4693 5594 5856 820
Size: 197 926 2750 901 262 1553
Fragments arranged by size:
2750 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4380 493
Size: 3887 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6589
SphI G_CATG'C
Cuts at: 1132 3947 1132
Size: 2815 3774
SspI AAT'ATT
Cuts at: 5 52 4513 5
Size: 47 4461 2081
Fragments arranged by size:
4461 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6589
StyI C'CwwG_G
Cuts at: 513 1065 2432 2916 3403 4028 513
Size: 552 1367 484 487 625 3074
Fragments arranged by size:
3074 1367 625 552 487 484
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2429 2977 4616 4633 4786 4971 5416
Size: 1255 849 548 1639 17 153 185 445
Cuts at: 5416 325
Size: 1498
Fragments arranged by size:
1639 1498 1255 849 548 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3129 3252 4835
Size: 274 80 33 51 1443 1152 123 1583
Cuts at: 4835 6547 96
Size: 1712 138
Fragments arranged by size:
1712 1583 1443 1152 274 138 123 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2957 2989 3640 3672 6549 6581 2957
Size: 32 651 32 2877 32 2965
Fragments arranged by size:
2965 2877 651 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6589
XbaI T'CTAG_A
Cuts at: 3925 3925
Size: 6589
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2815 2903 3826 2815
Size: 88 923 5578
Fragments arranged by size:
5578 923 88
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6589
XmaI C'CCGG_G
Cuts at: 1870 3914 1870
Size: 2044 4545
XmnI GAAnn'nnTTC
Cuts at: 2139 4718 2139
Size: 2579 4010
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3307 4397 276
Size: 53 83 186 936 1773 1090 2468
Fragments arranged by size:
2468 1773 1090 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspXI PsrI RsrII SacI SacII
SapI SexAI SfiI SfoI SrfI SwaI