(Circular) (Six-base) MAPSORT of: pFN21AB8371.seq Check: 2137 from: 1 to: 6262 pFN21AB8371 6262 bp With 182 enzymes: SgfI * August 16, 2010 14:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4072 278 Size: 53 83 186 936 2536 2468 Fragments arranged by size: 2536 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2964 3605 1937 Size: 1027 641 4594 Fragments arranged by size: 4594 1027 641 Acc65I G'GTAC_C Cuts at: 3583 3583 Size: 6262 AclI AA'CG_TT Cuts at: 1563 4389 4762 1563 Size: 2826 373 3063 Fragments arranged by size: 3063 2826 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3567 4325 5709 5977 1632 Size: 1935 758 1384 268 1917 Fragments arranged by size: 1935 1917 1384 758 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6065 AflIII A'CryG_T Cuts at: 1203 2982 5161 6138 1203 Size: 1779 2179 977 1327 Fragments arranged by size: 2179 1779 1327 977 AgeI A'CCGG_T Cuts at: 5093 5093 Size: 6262 AhdI GACnn_n'nnGTC Cuts at: 4991 4991 Size: 6262 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6262 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6262 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2151 2183 2151 Size: 32 6230 AlwNI CAG_nnn'CTG Cuts at: 4005 5577 4005 Size: 1572 4690 ApaI G_GGCC'C Cuts at: 3514 3514 Size: 6262 ApaLI G'TGCA_C Cuts at: 4319 5475 4319 Size: 1156 5106 ApoI r'AATT_y Cuts at: 1493 2242 3571 3797 3949 6058 1493 Size: 749 1329 226 152 2109 1697 Fragments arranged by size: 2109 1697 1329 749 226 152 AseI AT'TA_AT Cuts at: 160 1032 4816 160 Size: 872 3784 1606 Fragments arranged by size: 3784 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6262 AvaI C'yCGr_G Cuts at: 1870 1943 2025 3587 1870 Size: 73 82 1562 4545 Fragments arranged by size: 4545 1562 82 73 BamHI G'GATC_C Cuts at: 3592 3959 3592 Size: 367 5895 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3583 5038 618 Size: 325 217 627 1796 1455 1842 Fragments arranged by size: 1842 1796 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3514 3581 1391 Size: 2123 67 4072 Fragments arranged by size: 4072 2123 67 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5291 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4453 4487 1903 Size: 34 2516 34 3678 Fragments arranged by size: 3678 2516 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4154 5364 4154 Size: 1210 5052 BclI T'GATC_A Cuts at: 1548 2536 1548 Size: 988 5274 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4873 136 Size: 107 122 71 631 605 3201 1525 Fragments arranged by size: 3201 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6257 6257 Size: 6262 BlpI GC'TnA_GC Cuts at: 3679 3679 Size: 6262 Bme1580I G_kGCm'C Cuts at: 1790 3514 4323 5479 1790 Size: 1724 809 1156 2573 Fragments arranged by size: 2573 1724 1156 809 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6262 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3993 4951 442 Size: 416 53 83 399 24 2576 958 1753 Fragments arranged by size: 2576 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6262 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2541 3045 3432 4922 1239 Size: 1302 504 387 1490 2579 Fragments arranged by size: 2579 1490 1302 504 387 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2484 3076 4387 5252 5550 2484 Size: 592 1311 865 298 3196 Fragments arranged by size: 3196 1311 865 592 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4925 882 Size: 617 3426 2219 Fragments arranged by size: 3426 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4053 493 Size: 711 2849 2702 Fragments arranged by size: 2849 2702 711 BsaBI GATnn'nnATC Cuts at: 6256 6256 Size: 6262 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4069 4451 5086 275 Size: 53 83 186 936 2536 382 635 1451 Fragments arranged by size: 2536 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4695 5093 5367 5514 5925 4695 Size: 398 274 147 411 5032 Fragments arranged by size: 5032 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2151 2181 2550 2580 1178 Size: 30 165 30 748 30 369 30 4860 Fragments arranged by size: 4860 748 369 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2852 1186 Size: 1666 4596 BseYI C'CCAG_C Cuts at: 2131 2397 3248 5465 5961 2131 Size: 266 851 2217 496 2432 Fragments arranged by size: 2432 2217 851 496 266 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5689 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3712 4473 5501 1540 Size: 275 186 1711 761 1028 2301 Fragments arranged by size: 2301 1711 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4323 4408 5479 4323 Size: 85 1071 5106 Fragments arranged by size: 5106 1071 85 BsmI GAATG_Cn' Cuts at: 3773 3866 3773 Size: 93 6169 BsmBI CGTCTCn'nnnn_ Cuts at: 2966 2966 Size: 6262 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3514 3581 4323 4408 5479 1391 Size: 399 1724 67 742 85 1071 2174 Fragments arranged by size: 2174 1724 1071 742 399 85 67 BspEI T'CCGG_A Cuts at: 5925 5925 Size: 6262 BspHI T'CATG_A Cuts at: 2905 4151 5881 2905 Size: 1246 1730 3286 Fragments arranged by size: 3286 1730 1246 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2203 3617 844 Size: 1359 1414 3489 Fragments arranged by size: 3489 1414 1359 BsrBI CCG'CTC Cuts at: 1394 1823 4149 1394 Size: 429 2326 3507 Fragments arranged by size: 3507 2326 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2761 4757 4931 65 Size: 1183 1513 1996 174 1396 Fragments arranged by size: 1996 1513 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4906 5093 1700 Size: 110 3096 187 2869 Fragments arranged by size: 3096 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2478 2985 96 Size: 2382 507 3373 Fragments arranged by size: 3373 2382 507 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6262 BssSI C'ACGA_G Cuts at: 2417 4322 5334 2417 Size: 1905 1012 3345 Fragments arranged by size: 3345 1905 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6262 BstBI TT'CG_AA Cuts at: 5066 5066 Size: 6262 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6262 BstXI CCAn_nnnn'nTGG Cuts at: 3082 3341 3082 Size: 259 6003 BstYI r'GATC_y Cuts at: 1972 2221 3592 3959 4344 4361 5813 5899 5911 Size: 249 1371 367 385 17 1452 86 12 Cuts at: 5911 6257 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1371 385 367 346 249 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5710 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5523 Fragments arranged by size: 5523 683 56 BtsI GCAGTG_nn' Cuts at: 2295 3346 3778 4572 4592 2295 Size: 1051 432 794 20 3965 Fragments arranged by size: 3965 1051 794 432 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6262 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6227 DraI TTT'AAA Cuts at: 3566 3566 Size: 6262 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5269 809 Size: 507 3953 1802 Fragments arranged by size: 3953 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3709 4598 6227 Size: 54 1425 50 275 18 1879 889 1629 Cuts at: 6227 8 Size: 43 Fragments arranged by size: 1879 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3709 1537 Size: 275 1897 4090 Fragments arranged by size: 4090 1897 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4192 1353 Size: 2839 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4849 5223 5369 387 Size: 1064 138 3260 374 146 1280 Fragments arranged by size: 3260 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2541 3045 3432 3567 4325 4922 5709 Size: 393 909 504 387 135 758 597 787 Cuts at: 5709 5977 1239 Size: 268 1524 Fragments arranged by size: 1524 909 787 758 597 504 393 387 268 135 EcoRI G'AATT_C Cuts at: 2242 3571 3949 2242 Size: 1329 378 4555 Fragments arranged by size: 4555 1329 378 FspI TGC'GCA Cuts at: 4768 4768 Size: 6262 HaeII r_GCGC'y Cuts at: 1678 5409 1678 Size: 3731 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3178 3210 4903 4935 4977 Size: 32 135 32 1606 32 1693 32 42 Cuts at: 4977 5009 1373 Size: 32 2626 Fragments arranged by size: 2626 1693 1606 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2878 3606 3852 669 Size: 988 281 940 728 246 3079 Fragments arranged by size: 3079 988 940 728 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6262 HpaI GTT'AAC Cuts at: 3852 3852 Size: 6262 KpnI G_GTAC'C Cuts at: 3587 3587 Size: 6262 MfeI C'AATT_G Cuts at: 3861 3861 Size: 6262 MluI A'CGCG_T Cuts at: 6138 6138 Size: 6262 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5376 5560 1305 Size: 4071 184 2007 Fragments arranged by size: 4071 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4440 Fragments arranged by size: 4440 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2910 3180 3278 4220 4579 4738 518 Size: 648 1744 270 98 942 359 159 2042 Fragments arranged by size: 2042 1744 942 648 359 270 159 98 MspA1I CmG'CkG Cuts at: 1606 3678 4355 5503 5748 1606 Size: 2072 677 1148 245 2120 Fragments arranged by size: 2120 2072 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5710 NdeI CA'TA_TG Cuts at: 387 2208 387 Size: 1821 4441 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6262 NotI GC'GGCC_GC Cuts at: 3709 3709 Size: 6262 NspI r_CATG'y Cuts at: 1132 1229 2986 3620 5165 1132 Size: 97 1757 634 1545 2229 Fragments arranged by size: 2229 1757 1545 634 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6262 PciI A'CATG_T Cuts at: 2982 5161 2982 Size: 2179 4083 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6262 PmeI GTTT'AAAC Cuts at: 3566 3566 Size: 6262 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2151 2183 2508 2540 4331 4363 5844 5876 2151 Size: 32 325 32 1791 32 1481 32 2537 Fragments arranged by size: 2537 1791 1481 325 32 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2658 1613 Size: 1045 5217 PsiI TTA'TAA Cuts at: 3832 3832 Size: 6262 PspOMI G'GGCC_C Cuts at: 3510 3510 Size: 6262 PstI C_TGCA'G Cuts at: 830 3143 3614 830 Size: 2313 471 3478 Fragments arranged by size: 3478 2313 471 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6262 SalI G'TCGA_C Cuts at: 1936 3604 1936 Size: 1668 4594 SbfI CC_TGCA'GG Cuts at: 3614 3614 Size: 6262 ScaI AGT'ACT Cuts at: 4510 6222 4510 Size: 1712 4550 SexAI A'CCwGG_T Cuts at: 2103 2103 Size: 6262 SfcI C'TryA_G Cuts at: 826 1045 3108 3139 3610 4745 5426 5617 826 Size: 219 2063 31 471 1135 681 191 1471 Fragments arranged by size: 2063 1471 1135 681 471 219 191 31 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6262 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6262 SmaI CCC'GGG Cuts at: 1872 3589 1872 Size: 1717 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2499 3091 4366 5267 5529 820 Size: 197 926 556 592 1275 901 262 1553 Fragments arranged by size: 1553 1275 926 901 592 556 262 197 SnaBI TAC'GTA Cuts at: 493 4053 493 Size: 3560 2702 SpeI A'CTAG_T Cuts at: 152 2257 152 Size: 2105 4157 SphI G_CATG'C Cuts at: 1132 3620 1132 Size: 2488 3774 SspI AAT'ATT Cuts at: 5 52 4186 5 Size: 47 4134 2081 Fragments arranged by size: 4134 2081 47 StuI AGG'CCT Cuts at: 1517 3380 1517 Size: 1863 4399 StyI C'CwwG_G Cuts at: 513 1065 2461 3701 513 Size: 552 1396 1240 3074 Fragments arranged by size: 3074 1396 1240 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4289 4306 4459 4644 5089 325 Size: 1255 2709 17 153 185 445 1498 Fragments arranged by size: 2709 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2478 2985 3518 Size: 274 80 33 51 1443 501 507 533 Cuts at: 3518 4508 6220 96 Size: 990 1712 138 Fragments arranged by size: 1712 1443 990 533 507 501 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2167 2199 3456 3488 6222 6254 2167 Size: 32 1257 32 2734 32 2175 Fragments arranged by size: 2734 2175 1257 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6262 XbaI T'CTAG_A Cuts at: 3598 3598 Size: 6262 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6262 XmaI C'CCGG_G Cuts at: 1870 3587 1870 Size: 1717 4545 XmnI GAAnn'nnTTC Cuts at: 4391 4391 Size: 6262 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4070 276 Size: 53 83 186 936 2536 2468 Fragments arranged by size: 2536 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI