Restriction map for
pFN21AB8368
(Circular) (Six-base) MAPSORT of: pFN21AB8368.seq Check: 5590 from: 1 to: 6571
pFN21AB8368 6571 bp
With 182 enzymes: SgfI *
February 27, 2009 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2069 2147 3326 2069
Size: 78 1179 5314
Fragments arranged by size:
5314 1179 78
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4381 278
Size: 53 83 186 936 2845 2468
Fragments arranged by size:
2845 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3914 1937
Size: 1977 4594
Acc65I G'GTAC_C
Cuts at: 3892 3892
Size: 6571
AclI AA'CG_TT
Cuts at: 1563 4698 5071 1563
Size: 3135 373 3063
Fragments arranged by size:
3135 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2892 4634 6018 6286 1632
Size: 1260 1742 1384 268 1917
Fragments arranged by size:
1917 1742 1384 1260 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6374
AflIII A'CryG_T
Cuts at: 1203 5470 6447 1203
Size: 4267 977 1327
Fragments arranged by size:
4267 1327 977
AgeI A'CCGG_T
Cuts at: 2243 5402 2243
Size: 3159 3412
AhdI GACnn_n'nnGTC
Cuts at: 5300 5300
Size: 6571
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6571
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6571
AlwNI CAG_nnn'CTG
Cuts at: 2819 4314 5886 2819
Size: 1495 1572 3504
Fragments arranged by size:
3504 1572 1495
ApaI G_GGCC'C
Cuts at: 3332 3332
Size: 6571
ApaLI G'TGCA_C
Cuts at: 3339 4628 5784 3339
Size: 1289 1156 4126
Fragments arranged by size:
4126 1289 1156
ApoI r'AATT_y
Cuts at: 1493 2511 3880 4106 4258 6367 1493
Size: 1018 1369 226 152 2109 1697
Fragments arranged by size:
2109 1697 1369 1018 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5125 160
Size: 872 4093 1606
Fragments arranged by size:
4093 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6571
AvaI C'yCGr_G
Cuts at: 1870 1943 2525 3614 3896 1870
Size: 73 582 1089 282 4545
Fragments arranged by size:
4545 1089 582 282 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2635 2668 2697 2730 2635
Size: 33 29 33 6476
Fragments arranged by size:
6476 33 33 29
BamHI G'GATC_C
Cuts at: 3034 3233 3841 3901 4268 3034
Size: 199 608 60 367 5337
Fragments arranged by size:
5337 608 367 199 60
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3685 3892 5347 618
Size: 325 217 627 1898 207 1455 1842
Fragments arranged by size:
1898 1842 1455 627 325 217 207
BanII G_rGCy'C
Cuts at: 1391 3332 3890 1391
Size: 1941 558 4072
Fragments arranged by size:
4072 1941 558
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2433 928
Size: 971 534 5066
Fragments arranged by size:
5066 971 534
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4762 4796 1903
Size: 34 2825 34 3678
Fragments arranged by size:
3678 2825 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2636 3463 4463 5673 2636
Size: 827 1000 1210 3534
Fragments arranged by size:
3534 1210 1000 827
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6571
BfrBI ATG'CAT
Cuts at: 2221 2221
Size: 6571
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2268 3737 5182
Size: 107 122 71 631 605 596 1469 1445
Cuts at: 5182 136
Size: 1525
Fragments arranged by size:
1525 1469 1445 631 605 596 122 107
71
BglII A'GATC_T
Cuts at: 2100 6566 2100
Size: 4466 2105
BlpI GC'TnA_GC
Cuts at: 3988 3988
Size: 6571
Bme1580I G_kGCm'C
Cuts at: 1790 3332 3343 4632 5788 1790
Size: 1542 11 1289 1156 2573
Fragments arranged by size:
2573 1542 1289 1156 11
BmgBI CAC'GTC
Cuts at: 1318 2365 1318
Size: 1047 5524
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3181 4302 5260
Size: 416 53 83 399 24 1764 1121 958
Cuts at: 5260 442
Size: 1753
Fragments arranged by size:
1764 1753 1121 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6571
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2231 2231
Size: 6571
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2668 3800 5231 1239
Size: 1429 1132 1431 2579
Fragments arranged by size:
2579 1431 1429 1132
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4696 5561 5859 4696
Size: 865 298 5408
Fragments arranged by size:
5408 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2463 5234 882
Size: 617 964 2771 2219
Fragments arranged by size:
2771 2219 964 617
BsaAI yAC'GTr
Cuts at: 493 1204 4362 493
Size: 711 3158 2702
Fragments arranged by size:
3158 2702 711
BsaBI GATnn'nnATC
Cuts at: 2847 6565 2847
Size: 3718 2853
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4378 4760 5395 275
Size: 53 83 186 936 2845 382 635 1451
Fragments arranged by size:
2845 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2243 5004 5402 5676 5823 6234 2243
Size: 2761 398 274 147 411 2580
Fragments arranged by size:
2761 2580 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6346
Fragments arranged by size:
6346 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6571
BseYI C'CCAG_C
Cuts at: 2558 3266 3426 3820 5774 6270 2558
Size: 708 160 394 1954 496 2859
Fragments arranged by size:
2859 1954 708 496 394 160
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3829 1163
Size: 573 2093 3905
Fragments arranged by size:
3905 2093 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4021 4782 5810 1540
Size: 275 186 2020 761 1028 2301
Fragments arranged by size:
2301 2020 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3343 4632 4717 5788 3343
Size: 1289 85 1071 4126
Fragments arranged by size:
4126 1289 1071 85
BsmI GAATG_Cn'
Cuts at: 4082 4175 4082
Size: 93 6478
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3332 3343 3890 4632 4717 5788 1391
Size: 399 1542 11 547 742 85 1071 2174
Fragments arranged by size:
2174 1542 1071 742 547 399 85 11
BspEI T'CCGG_A
Cuts at: 6234 6234
Size: 6571
BspHI T'CATG_A
Cuts at: 2304 4460 6190 2304
Size: 2156 1730 2685
Fragments arranged by size:
2685 2156 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2069 2147 2807 3326 3356 3926 844
Size: 1225 78 660 519 30 570 3489
Fragments arranged by size:
3489 1225 660 570 519 78 30
BsrBI CCG'CTC
Cuts at: 1394 1823 4458 1394
Size: 429 2635 3507
Fragments arranged by size:
3507 2635 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3569 5066 5240 65
Size: 1183 2321 1497 174 1396
Fragments arranged by size:
2321 1497 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2243 3343 5215 5402 1700
Size: 110 433 1100 1872 187 2869
Fragments arranged by size:
2869 1872 1100 433 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6571
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6571
BssSI C'ACGA_G
Cuts at: 4631 5643 4631
Size: 1012 5559
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6571
BstBI TT'CG_AA
Cuts at: 5375 5375
Size: 6571
BstEII G'GTnAC_C
Cuts at: 1184 2794 1184
Size: 1610 4961
BstXI CCAn_nnnn'nTGG
Cuts at: 2558 3031 3824 2558
Size: 473 793 5305
Fragments arranged by size:
5305 793 473
BstYI r'GATC_y
Cuts at: 1972 2100 2547 2718 3034 3233 3284 3691 3841
Size: 128 447 171 316 199 51 407 150
Cuts at: 3841 3901 4268 4653 4670 6122 6208 6220 6566
Size: 60 367 385 17 1452 86 12 346
Cuts at: 6566 1972
Size: 1977
Fragments arranged by size:
1977 1452 447 407 385 367 346 316
199 171 150 128 86 60 51 17
12
Bsu36I CC'TnA_GG
Cuts at: 3135 3135
Size: 6571
BtgI C'CryG_G
Cuts at: 513 1065 2638 513
Size: 552 1573 4446
Fragments arranged by size:
4446 1573 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5832
Fragments arranged by size:
5832 683 56
BtsI GCAGTG_nn'
Cuts at: 2775 3789 4087 4881 4901 2775
Size: 1014 298 794 20 4445
Fragments arranged by size:
4445 1014 794 298 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6571
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6536
DraI TTT'AAA
Cuts at: 2107 3381 3875 2107
Size: 1274 494 4803
Fragments arranged by size:
4803 1274 494
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5578 809
Size: 507 4262 1802
Fragments arranged by size:
4262 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3345 3780 4018
Size: 54 1425 50 275 18 1515 435 238
Cuts at: 4018 4907 6536 8
Size: 889 1629 43
Fragments arranged by size:
1629 1515 1425 889 435 275 238 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4018 1537
Size: 275 2206 4090
Fragments arranged by size:
4090 2206 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3405 4501 1353
Size: 2052 1096 3423
Fragments arranged by size:
3423 2052 1096
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2799 5158 5532 5678 387
Size: 1064 138 1210 2359 374 146 1280
Fragments arranged by size:
2359 1280 1210 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2668 2892 3800 4634 5231 6018 6286
Size: 393 1036 224 908 834 597 787 268
Cuts at: 6286 1239
Size: 1524
Fragments arranged by size:
1524 1036 908 834 787 597 393 268
224
EcoNI CCTnn'n_nnAGG
Cuts at: 2615 2615
Size: 6571
EcoO109I rG'GnC_Cy
Cuts at: 3181 3329 3739 3181
Size: 148 410 6013
Fragments arranged by size:
6013 410 148
EcoRI G'AATT_C
Cuts at: 3880 4258 3880
Size: 378 6193
FspI TGC'GCA
Cuts at: 5077 5077
Size: 6571
HaeII r_GCGC'y
Cuts at: 1678 5718 1678
Size: 4040 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3032 3064 5212 5244 5286
Size: 32 135 32 1460 32 2148 32 42
Cuts at: 5286 5318 1373
Size: 32 2626
Fragments arranged by size:
2626 2148 1460 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3550 3915 4161 669
Size: 988 281 1612 365 246 3079
Fragments arranged by size:
3079 1612 988 365 281 246
HindIII A'AGCT_T
Cuts at: 748 3445 748
Size: 2697 3874
HpaI GTT'AAC
Cuts at: 4161 4161
Size: 6571
KpnI G_GTAC'C
Cuts at: 3896 3896
Size: 6571
MfeI C'AATT_G
Cuts at: 4170 4170
Size: 6571
MluI A'CGCG_T
Cuts at: 6447 6447
Size: 6571
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2835 5685 5869 1305
Size: 1530 2850 184 2007
Fragments arranged by size:
2850 2007 1530 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3782 10
Size: 54 1425 343 1950 2799
Fragments arranged by size:
2799 1950 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2285 2306 3197 3228 3677 4529 4888
Size: 648 1119 21 891 31 449 852 359
Cuts at: 4888 5047 518
Size: 159 2042
Fragments arranged by size:
2042 1119 891 852 648 449 359 159
31 21
MspA1I CmG'CkG
Cuts at: 1606 2537 3597 3820 3987 4664 5812 6057 1606
Size: 931 1060 223 167 677 1148 245 2120
Fragments arranged by size:
2120 1148 1060 931 677 245 223 167
NcoI C'CATG_G
Cuts at: 513 1065 2638 513
Size: 552 1573 4446
Fragments arranged by size:
4446 1573 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6571
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6571
NotI GC'GGCC_GC
Cuts at: 4018 4018
Size: 6571
NsiI A_TGCA'T
Cuts at: 2223 2223
Size: 6571
NspI r_CATG'y
Cuts at: 1132 1229 3701 3852 3929 5474 1132
Size: 97 2472 151 77 1545 2229
Fragments arranged by size:
2472 2229 1545 151 97 77
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6571
PciI A'CATG_T
Cuts at: 5470 5470
Size: 6571
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2639 2819 1108
Size: 1531 180 4860
Fragments arranged by size:
4860 1531 180
PfoI T'CCnGG_A
Cuts at: 2086 2596 2086
Size: 510 6061
PmeI GTTT'AAAC
Cuts at: 3875 3875
Size: 6571
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4640 4672 6153 6185 4640
Size: 32 1481 32 5026
Fragments arranged by size:
5026 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3181 3181
Size: 6571
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6571
PsiI TTA'TAA
Cuts at: 4141 4141
Size: 6571
PspOMI G'GGCC_C
Cuts at: 3328 3328
Size: 6571
PstI C_TGCA'G
Cuts at: 830 3337 3923 830
Size: 2507 586 3478
Fragments arranged by size:
3478 2507 586
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6571
PvuII CAG'CTG
Cuts at: 2537 3820 2537
Size: 1283 5288
SalI G'TCGA_C
Cuts at: 1936 3913 1936
Size: 1977 4594
SanDI GG'GwC_CC
Cuts at: 3181 3181
Size: 6571
SbfI CC_TGCA'GG
Cuts at: 3337 3923 3337
Size: 586 5985
ScaI AGT'ACT
Cuts at: 4819 6531 4819
Size: 1712 4859
SfcI C'TryA_G
Cuts at: 826 1045 2785 3333 3919 5054 5735 5926 826
Size: 219 1740 548 586 1135 681 191 1471
Fragments arranged by size:
1740 1471 1135 681 586 548 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6571
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6571
SmaI CCC'GGG
Cuts at: 1872 3616 3898 1872
Size: 1744 282 4545
Fragments arranged by size:
4545 1744 282
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4675 5576 5838 820
Size: 197 926 2732 901 262 1553
Fragments arranged by size:
2732 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4362 493
Size: 3869 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6571
SphI G_CATG'C
Cuts at: 1132 3929 1132
Size: 2797 3774
SspI AAT'ATT
Cuts at: 5 52 3301 4495 5
Size: 47 3249 1194 2081
Fragments arranged by size:
3249 2081 1194 47
StuI AGG'CCT
Cuts at: 1517 2152 1517
Size: 635 5936
StyI C'CwwG_G
Cuts at: 513 1065 2638 2889 3522 3645 4010 513
Size: 552 1573 251 633 123 365 3074
Fragments arranged by size:
3074 1573 633 552 365 251 123
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3018 3812 4598 4615 4768 4953 5398
Size: 1255 1438 794 786 17 153 185 445
Cuts at: 5398 325
Size: 1498
Fragments arranged by size:
1498 1438 1255 794 786 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2277 4817 6529
Size: 274 80 33 51 1443 300 2540 1712
Cuts at: 6529 96
Size: 138
Fragments arranged by size:
2540 1712 1443 300 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3173 3205 6531 6563 3173
Size: 32 3326 32 3181
Fragments arranged by size:
3326 3181 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6571
XbaI T'CTAG_A
Cuts at: 3404 3907 3404
Size: 503 6068
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2326 2326
Size: 6571
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6571
XmaI C'CCGG_G
Cuts at: 1870 3614 3896 1870
Size: 1744 282 4545
Fragments arranged by size:
4545 1744 282
XmnI GAAnn'nnTTC
Cuts at: 4700 4700
Size: 6571
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4379 276
Size: 53 83 186 936 2845 2468
Fragments arranged by size:
2845 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BbvCI Bpu10I BsiWI
BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI
PsrI RsrII SacI SacII SapI SexAI SfiI SfoI
SrfI SwaI