Restriction map for
pFN21AB8363
(Circular) (Six-base) MAPSORT of: pFN21AB8363.seq Check: 9395 from: 1 to: 6214
pFN21AB8363 6214 bp
With 182 enzymes: SgfI *
February 27, 2009 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4024 278
Size: 53 83 186 936 2488 2468
Fragments arranged by size:
2488 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3557 1937
Size: 1620 4594
Acc65I G'GTAC_C
Cuts at: 3099 3535 3099
Size: 436 5778
AclI AA'CG_TT
Cuts at: 1563 4341 4714 1563
Size: 2778 373 3063
Fragments arranged by size:
3063 2778 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2079 4277 5661 5929 1632
Size: 447 2198 1384 268 1917
Fragments arranged by size:
2198 1917 1384 447 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6017
AflIII A'CryG_T
Cuts at: 1203 2562 3511 5113 6090 1203
Size: 1359 949 1602 977 1327
Fragments arranged by size:
1602 1359 1327 977 949
AgeI A'CCGG_T
Cuts at: 5045 5045
Size: 6214
AhdI GACnn_n'nnGTC
Cuts at: 2935 4943 2935
Size: 2008 4206
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6214
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2399 1184
Size: 1215 4999
AlwNI CAG_nnn'CTG
Cuts at: 2153 2223 2927 3957 5529 2153
Size: 70 704 1030 1572 2838
Fragments arranged by size:
2838 1572 1030 704 70
ApaI G_GGCC'C
Cuts at: 3306 3306
Size: 6214
ApaLI G'TGCA_C
Cuts at: 4271 5427 4271
Size: 1156 5058
ApoI r'AATT_y
Cuts at: 1493 2252 3239 3253 3523 3749 3901 6010 1493
Size: 759 987 14 270 226 152 2109 1697
Fragments arranged by size:
2109 1697 987 759 270 226 152 14
AseI AT'TA_AT
Cuts at: 160 1032 4768 160
Size: 872 3736 1606
Fragments arranged by size:
3736 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6214
AvaI C'yCGr_G
Cuts at: 1870 1943 3215 3539 1870
Size: 73 1272 324 4545
Fragments arranged by size:
4545 1272 324 73
BamHI G'GATC_C
Cuts at: 3544 3911 3544
Size: 367 5847
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3099 3535 4990 618
Size: 325 217 627 1312 436 1455 1842
Fragments arranged by size:
1842 1455 1312 627 436 325 217
BanII G_rGCy'C
Cuts at: 1391 2424 3306 3533 1391
Size: 1033 882 227 4072
Fragments arranged by size:
4072 1033 882 227
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3392 928
Size: 971 1493 3750
Fragments arranged by size:
3750 1493 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4405 4439 1903
Size: 34 2468 34 3678
Fragments arranged by size:
3678 2468 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4106 5316 4106
Size: 1210 5004
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6214
BfrBI ATG'CAT
Cuts at: 2386 2672 2386
Size: 286 5928
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4825 136
Size: 107 122 71 631 605 3153 1525
Fragments arranged by size:
3153 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6209 6209
Size: 6214
BlpI GC'TnA_GC
Cuts at: 3631 3631
Size: 6214
Bme1580I G_kGCm'C
Cuts at: 1790 2135 3306 4275 5431 1790
Size: 345 1171 969 1156 2573
Fragments arranged by size:
2573 1171 1156 969 345
BmgBI CAC'GTC
Cuts at: 1318 2563 1318
Size: 1245 4969
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3158 3945 4903
Size: 416 53 83 399 24 1741 787 958
Cuts at: 4903 442
Size: 1753
Fragments arranged by size:
1753 1741 958 787 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6214
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2295 4874 1239
Size: 1056 2579 2579
Fragments arranged by size:
2579 2579 1056
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2290 2635 4339 5204 5502 2290
Size: 345 1704 865 298 3002
Fragments arranged by size:
3002 1704 865 345 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4877 882
Size: 617 3378 2219
Fragments arranged by size:
3378 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3382 4005 493
Size: 711 2178 623 2702
Fragments arranged by size:
2702 2178 711 623
BsaBI GATnn'nnATC
Cuts at: 2210 6208 2210
Size: 3998 2216
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4021 4403 5038 275
Size: 53 83 186 936 2488 382 635 1451
Fragments arranged by size:
2488 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4647 5045 5319 5466 5877 4647
Size: 398 274 147 411 4984
Fragments arranged by size:
4984 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5989
Fragments arranged by size:
5989 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2084 2546 2675 1186
Size: 898 462 129 4725
Fragments arranged by size:
4725 898 462 129
BseYI C'CCAG_C
Cuts at: 5417 5913 5417
Size: 496 5718
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3336 1163
Size: 573 1600 4041
Fragments arranged by size:
4041 1600 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3664 4425 5453 1540
Size: 275 186 1663 761 1028 2301
Fragments arranged by size:
2301 1663 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2424 4275 4360 5431 2424
Size: 1851 85 1071 3207
Fragments arranged by size:
3207 1851 1071 85
BsmI GAATG_Cn'
Cuts at: 3725 3818 3725
Size: 93 6121
BsmBI CGTCTCn'nnnn_
Cuts at: 3364 3364
Size: 6214
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2135 2424 3306 3533 4275 4360 5431
Size: 399 345 289 882 227 742 85 1071
Cuts at: 5431 1391
Size: 2174
Fragments arranged by size:
2174 1071 882 742 399 345 289 227
85
BspEI T'CCGG_A
Cuts at: 5877 5877
Size: 6214
BspHI T'CATG_A
Cuts at: 4103 5833 4103
Size: 1730 4484
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3569 844
Size: 2725 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4101 1394
Size: 429 2278 3507
Fragments arranged by size:
3507 2278 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2967 3389 4709 4883 65
Size: 1183 1719 422 1320 174 1396
Fragments arranged by size:
1719 1396 1320 1183 422 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4858 5045 1700
Size: 110 3048 187 2869
Fragments arranged by size:
3048 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6214
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6214
BssSI C'ACGA_G
Cuts at: 4274 5286 4274
Size: 1012 5202
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2223 794
Size: 1429 4785
BstBI TT'CG_AA
Cuts at: 5018 5018
Size: 6214
BstEII G'GTnAC_C
Cuts at: 1184 2536 2870 1184
Size: 1352 334 4528
Fragments arranged by size:
4528 1352 334
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2424 2011
Size: 413 5801
BstYI r'GATC_y
Cuts at: 1972 2268 2619 3544 3911 4296 4313 5765 5851
Size: 296 351 925 367 385 17 1452 86
Cuts at: 5851 5863 6209 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 925 385 367 351 346 296
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2188 3307 3333 513
Size: 552 939 184 1119 26 3394
Fragments arranged by size:
3394 1119 939 552 184 26
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3313 487
Size: 683 56 2087 3388
Fragments arranged by size:
3388 2087 683 56
BtsI GCAGTG_nn'
Cuts at: 2224 2378 3730 4524 4544 2224
Size: 154 1352 794 20 3894
Fragments arranged by size:
3894 1352 794 154 20
ClaI AT'CG_AT
Cuts at: 1554 3153 1554
Size: 1599 4615
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6179
DraI TTT'AAA
Cuts at: 3518 3518
Size: 6214
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5221 809
Size: 507 3905 1802
Fragments arranged by size:
3905 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3661 4550 6179
Size: 54 1425 50 275 18 1831 889 1629
Cuts at: 6179 8
Size: 43
Fragments arranged by size:
1831 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3661 1537
Size: 275 1849 4090
Fragments arranged by size:
4090 1849 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2615 2805 2985 4144 1353
Size: 1262 190 180 1159 3423
Fragments arranged by size:
3423 1262 1159 190 180
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4801 5175 5321 387
Size: 1064 138 3212 374 146 1280
Fragments arranged by size:
3212 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2422 2422
Size: 6214
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2079 2295 4277 4874 5661 5929 1239
Size: 393 447 216 1982 597 787 268 1524
Fragments arranged by size:
1982 1524 787 597 447 393 268 216
EcoO109I rG'GnC_Cy
Cuts at: 2150 2465 3303 2150
Size: 315 838 5061
Fragments arranged by size:
5061 838 315
EcoRI G'AATT_C
Cuts at: 2252 3523 3901 2252
Size: 1271 378 4565
Fragments arranged by size:
4565 1271 378
FspI TGC'GCA
Cuts at: 3128 4720 3128
Size: 1592 4622
HaeII r_GCGC'y
Cuts at: 1678 5361 1678
Size: 3683 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2120 2152 2166 2198 2253
Size: 32 135 32 548 32 14 32 55
Cuts at: 2253 2285 2908 2921 2940 2953 4855 4887 4929
Size: 32 623 13 19 13 1902 32 42
Cuts at: 4929 4961 1373
Size: 32 2626
Fragments arranged by size:
2626 1902 623 548 135 55 42 32
32 32 32 32 32 32 19 14
13 13
HincII GTy'rAC
Cuts at: 669 1657 1938 2568 3558 3804 669
Size: 988 281 630 990 246 3079
Fragments arranged by size:
3079 990 988 630 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6214
HpaI GTT'AAC
Cuts at: 3804 3804
Size: 6214
KpnI G_GTAC'C
Cuts at: 3103 3539 3103
Size: 436 5778
MfeI C'AATT_G
Cuts at: 3813 3813
Size: 6214
MluI A'CGCG_T
Cuts at: 6090 6090
Size: 6214
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2508 5328 5512 1305
Size: 1203 2820 184 2007
Fragments arranged by size:
2820 2007 1203 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4392
Fragments arranged by size:
4392 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2381 2982 3332 3338 3447 4172 4531
Size: 648 1215 601 350 6 109 725 359
Cuts at: 4531 4690 518
Size: 159 2042
Fragments arranged by size:
2042 1215 725 648 601 359 350 159
109 6
MspA1I CmG'CkG
Cuts at: 1606 3630 4307 5455 5700 1606
Size: 2024 677 1148 245 2120
Fragments arranged by size:
2120 2024 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3333 513
Size: 552 939 1329 3394
Fragments arranged by size:
3394 1329 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6214
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6214
NotI GC'GGCC_GC
Cuts at: 3661 3661
Size: 6214
NsiI A_TGCA'T
Cuts at: 2388 2674 2388
Size: 286 5928
NspI r_CATG'y
Cuts at: 1132 1229 3097 3466 3572 5117 1132
Size: 97 1868 369 106 1545 2229
Fragments arranged by size:
2229 1868 1545 369 106 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6214
PciI A'CATG_T
Cuts at: 5113 5113
Size: 6214
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2153 1108
Size: 1045 5169
PmeI GTTT'AAAC
Cuts at: 3518 3518
Size: 6214
PmlI CAC'GTG
Cuts at: 3382 3382
Size: 6214
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4283 4315 5796 5828 4283
Size: 32 1481 32 4669
Fragments arranged by size:
4669 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2150 2150
Size: 6214
PshAI GACnn'nnGTC
Cuts at: 1613 3501 1613
Size: 1888 4326
PsiI TTA'TAA
Cuts at: 2037 3784 2037
Size: 1747 4467
PspOMI G'GGCC_C
Cuts at: 3302 3302
Size: 6214
PspXI vC'TCGA_Gb
Cuts at: 3215 3215
Size: 6214
PstI C_TGCA'G
Cuts at: 830 2373 3566 830
Size: 1543 1193 3478
Fragments arranged by size:
3478 1543 1193
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6214
SacI G_AGCT'C
Cuts at: 2424 2424
Size: 6214
SalI G'TCGA_C
Cuts at: 1936 3556 1936
Size: 1620 4594
SapI GCTCTTCn'nnn_
Cuts at: 2615 2615
Size: 6214
SbfI CC_TGCA'GG
Cuts at: 3566 3566
Size: 6214
ScaI AGT'ACT
Cuts at: 4462 6174 4462
Size: 1712 4502
SexAI A'CCwGG_T
Cuts at: 2146 2146
Size: 6214
SfcI C'TryA_G
Cuts at: 826 1045 2369 3562 4697 5378 5569 826
Size: 219 1324 1193 1135 681 191 1471
Fragments arranged by size:
1471 1324 1193 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6214
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6214
SmaI CCC'GGG
Cuts at: 1872 3541 1872
Size: 1669 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2305 2614 3215 4318 5219 5481
Size: 197 926 362 309 601 1103 901 262
Cuts at: 5481 820
Size: 1553
Fragments arranged by size:
1553 1103 926 901 601 362 309 262
197
SnaBI TAC'GTA
Cuts at: 493 4005 493
Size: 3512 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6214
SphI G_CATG'C
Cuts at: 1132 3097 3572 1132
Size: 1965 475 3774
Fragments arranged by size:
3774 1965 475
SspI AAT'ATT
Cuts at: 5 52 3196 4138 5
Size: 47 3144 942 2081
Fragments arranged by size:
3144 2081 942 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6214
StyI C'CwwG_G
Cuts at: 513 1065 2004 2656 3333 3653 513
Size: 552 939 652 677 320 3074
Fragments arranged by size:
3074 939 677 652 552 320
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3459 4241 4258 4411 4596 5041 325
Size: 1255 1879 782 17 153 185 445 1498
Fragments arranged by size:
1879 1498 1255 782 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3324 4460 6172
Size: 274 80 33 51 1443 1347 1136 1712
Cuts at: 6172 96
Size: 138
Fragments arranged by size:
1712 1443 1347 1136 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2203 2235 3419 3451 6174 6206 2203
Size: 32 1184 32 2723 32 2211
Fragments arranged by size:
2723 2211 1184 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3119 1316
Size: 1803 4411
XbaI T'CTAG_A
Cuts at: 3550 3550
Size: 6214
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3020 3020
Size: 6214
XhoI C'TCGA_G
Cuts at: 1943 3215 1943
Size: 1272 4942
XmaI C'CCGG_G
Cuts at: 1870 3539 1870
Size: 1669 4545
XmnI GAAnn'nnTTC
Cuts at: 4343 4343
Size: 6214
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4022 276
Size: 53 83 186 936 2488 2468
Fragments arranged by size:
2488 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI SacI SalI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PsrI PvuII RsrII SacII SanDI SfiI
SfoI SrfI SwaI