Restriction map for
pFN21AB8362
(Circular) (Six-base) MAPSORT of: pFN21AB8362.seq Check: 3059 from: 1 to: 6034
pFN21AB8362 6034 bp
With 182 enzymes: SgfI *
February 27, 2009 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2366 2487 2951 3844
Size: 53 83 186 936 830 121 464 893
Cuts at: 3844 278
Size: 2468
Fragments arranged by size:
2468 936 893 830 464 186 121 83
53
AccI GT'mk_AC
Cuts at: 1937 2400 2645 3377 1937
Size: 463 245 732 4594
Fragments arranged by size:
4594 732 463 245
Acc65I G'GTAC_C
Cuts at: 2226 3355 2226
Size: 1129 4905
AclI AA'CG_TT
Cuts at: 1563 4161 4534 1563
Size: 2598 373 3063
Fragments arranged by size:
3063 2598 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3214 4097 5481 5749 1632
Size: 1582 883 1384 268 1917
Fragments arranged by size:
1917 1582 1384 883 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5837
AflIII A'CryG_T
Cuts at: 1203 3201 4933 5910 1203
Size: 1998 1732 977 1327
Fragments arranged by size:
1998 1732 1327 977
AgeI A'CCGG_T
Cuts at: 4865 4865
Size: 6034
AhdI GACnn_n'nnGTC
Cuts at: 4763 4763
Size: 6034
AleI CACnn'nnGTG
Cuts at: 1166 2607 1166
Size: 1441 4593
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3152 1184
Size: 1968 4066
AlwNI CAG_nnn'CTG
Cuts at: 3777 5349 3777
Size: 1572 4462
ApaI G_GGCC'C
Cuts at: 2391 2391
Size: 6034
ApaLI G'TGCA_C
Cuts at: 4091 5247 4091
Size: 1156 4878
ApoI r'AATT_y
Cuts at: 1493 3343 3569 3721 5830 1493
Size: 1850 226 152 2109 1697
Fragments arranged by size:
2109 1850 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4588 160
Size: 872 3556 1606
Fragments arranged by size:
3556 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6034
AvaI C'yCGr_G
Cuts at: 1870 1943 2089 2383 2684 3359 1870
Size: 73 146 294 301 675 4545
Fragments arranged by size:
4545 675 301 294 146 73
BamHI G'GATC_C
Cuts at: 2279 3364 3731 2279
Size: 1085 367 4582
Fragments arranged by size:
4582 1085 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2226 2566 3355 4810 618
Size: 325 217 627 439 340 789 1455 1842
Fragments arranged by size:
1842 1455 789 627 439 340 325 217
BanII G_rGCy'C
Cuts at: 1391 2391 2871 3033 3353 1391
Size: 1000 480 162 320 4072
Fragments arranged by size:
4072 1000 480 320 162
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5063
BbvCI CC'TCA_GC
Cuts at: 2096 2096
Size: 6034
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4225 4259 1903
Size: 34 2288 34 3678
Fragments arranged by size:
3678 2288 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3926 5136 3926
Size: 1210 4824
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6034
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2528 2724 4645
Size: 107 122 71 631 605 856 196 1921
Cuts at: 4645 136
Size: 1525
Fragments arranged by size:
1921 1525 856 631 605 196 122 107
71
BglII A'GATC_T
Cuts at: 6029 6029
Size: 6034
BlpI GC'TnA_GC
Cuts at: 3451 3451
Size: 6034
Bme1580I G_kGCm'C
Cuts at: 1790 2391 2571 2685 4095 5251 1790
Size: 601 180 114 1410 1156 2573
Fragments arranged by size:
2573 1410 1156 601 180 114
BmgBI CAC'GTC
Cuts at: 1318 2182 1318
Size: 864 5170
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3765 4723 442
Size: 416 53 83 399 24 2348 958 1753
Fragments arranged by size:
2348 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6034
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2247 2710 2247
Size: 463 5571
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2612 3007 3170 3178 3223 3295 4694 1239
Size: 1373 395 163 8 45 72 1399 2579
Fragments arranged by size:
2579 1399 1373 395 163 72 45 8
Bpu10I CC'TnA_GC
Cuts at: 2096 3212 2096
Size: 1116 4918
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2540 4159 5024 5322 2540
Size: 1619 865 298 3252
Fragments arranged by size:
3252 1619 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4697 882
Size: 617 3198 2219
Fragments arranged by size:
3198 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2680 3825 493
Size: 711 1476 1145 2702
Fragments arranged by size:
2702 1476 1145 711
BsaBI GATnn'nnATC
Cuts at: 6028 6028
Size: 6034
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2363 2484 2948 3841
Size: 53 83 186 936 830 121 464 893
Cuts at: 3841 4223 4858 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 893 830 635 464 382 186
121 83 53
BsaWI w'CCGG_w
Cuts at: 2696 4467 4865 5139 5286 5697 2696
Size: 1771 398 274 147 411 3033
Fragments arranged by size:
3033 1771 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2315 2345 2363 2393 3191
Size: 30 165 30 912 30 18 30 798
Cuts at: 3191 3221 3293 3323 1178
Size: 30 72 30 3889
Fragments arranged by size:
3889 912 798 165 72 30 30 30
30 30 30 18
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2153 2410 2629 3004 1186
Size: 967 257 219 375 4216
Fragments arranged by size:
4216 967 375 257 219
BseYI C'CCAG_C
Cuts at: 2031 2711 2722 5237 5733 2031
Size: 680 11 2515 496 2332
Fragments arranged by size:
2515 2332 680 496 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2240 1163
Size: 573 504 4957
Fragments arranged by size:
4957 573 504
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2523 3186 3484 4245 5273 1540
Size: 275 186 522 663 298 761 1028 2301
Fragments arranged by size:
2301 1028 761 663 522 298 275 186
BsiHKAI G_wGCw'C
Cuts at: 2061 4095 4180 5251 2061
Size: 2034 85 1071 2844
Fragments arranged by size:
2844 2034 1071 85
BsmI GAATG_Cn'
Cuts at: 3001 3545 3638 3001
Size: 544 93 5397
Fragments arranged by size:
5397 544 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2491 2491
Size: 6034
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2061 2391 2571 2685 2871 3033 3353
Size: 399 271 330 180 114 186 162 320
Cuts at: 3353 4095 4180 5251 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 330 320 271 186
180 162 114 85
BspEI T'CCGG_A
Cuts at: 2696 5697 2696
Size: 3001 3033
BspHI T'CATG_A
Cuts at: 3923 5653 3923
Size: 1730 4304
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3389 844
Size: 2545 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3921 1394
Size: 429 2098 3507
Fragments arranged by size:
3507 2098 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3025 4529 4703 65
Size: 1183 1777 1504 174 1396
Fragments arranged by size:
1777 1504 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2522 2738 2895 4678 4865 1700
Size: 110 712 216 157 1783 187 2869
Fragments arranged by size:
2869 1783 712 216 187 157 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6034
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6034
BssSI C'ACGA_G
Cuts at: 4094 5106 4094
Size: 1012 5022
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2862 794
Size: 2068 3966
BstBI TT'CG_AA
Cuts at: 4838 4838
Size: 6034
BstEII G'GTnAC_C
Cuts at: 1184 3319 1184
Size: 2135 3899
BstXI CCAn_nnnn'nTGG
Cuts at: 2609 2609
Size: 6034
BstYI r'GATC_y
Cuts at: 1972 2279 3364 3731 4116 4133 5585 5671 5683
Size: 307 1085 367 385 17 1452 86 12
Cuts at: 5683 6029 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1085 385 367 346 307 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2214 2242 2374 2608 3327 513
Size: 552 939 210 28 132 234 719 3220
Fragments arranged by size:
3220 939 719 552 234 210 132 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2484 487
Size: 683 56 1258 4037
Fragments arranged by size:
4037 1258 683 56
BtsI GCAGTG_nn'
Cuts at: 3550 4344 4364 3550
Size: 794 20 5220
Fragments arranged by size:
5220 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6034
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3007 3042 529
Size: 35 2443 35 3521
Fragments arranged by size:
3521 2443 35 35
DraI TTT'AAA
Cuts at: 3338 3338
Size: 6034
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5041 809
Size: 507 3725 1802
Fragments arranged by size:
3725 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2520 3183 3481
Size: 54 1425 50 275 18 690 663 298
Cuts at: 3481 4370 5999 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 690 663 298 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2520 3183 3481 1537
Size: 275 708 663 298 4090
Fragments arranged by size:
4090 708 663 298 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2459 2708 3964 1353
Size: 1106 249 1256 3423
Fragments arranged by size:
3423 1256 1106 249
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2033 4621 4995 5141 387
Size: 1064 138 444 2588 374 146 1280
Fragments arranged by size:
2588 1280 1064 444 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2612 3007 3170 3178 3214 3223 3295
Size: 393 980 395 163 8 36 9 72
Cuts at: 3295 4097 4694 5481 5749 1239
Size: 802 597 787 268 1524
Fragments arranged by size:
1524 980 802 787 597 395 393 268
163 72 36 9 8
EcoO109I rG'GnC_Cy
Cuts at: 2387 2388 3007 2387
Size: 1 619 5414
Fragments arranged by size:
5414 619 1
EcoRI G'AATT_C
Cuts at: 3343 3721 3343
Size: 378 5656
FspI TGC'GCA
Cuts at: 4540 4540
Size: 6034
HaeII r_GCGC'y
Cuts at: 1678 5181 1678
Size: 3503 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4675 4707 4749 4781 1373
Size: 32 135 32 3103 32 42 32 2626
Fragments arranged by size:
3103 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2646 3378 3624 669
Size: 988 281 708 732 246 3079
Fragments arranged by size:
3079 988 732 708 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6034
HpaI GTT'AAC
Cuts at: 3624 3624
Size: 6034
KpnI G_GTAC'C
Cuts at: 2230 3359 2230
Size: 1129 4905
MfeI C'AATT_G
Cuts at: 3633 3633
Size: 6034
MluI A'CGCG_T
Cuts at: 5910 5910
Size: 6034
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2258 5148 5332 1305
Size: 953 2890 184 2007
Fragments arranged by size:
2890 2007 953 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4212
Fragments arranged by size:
4212 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2607 3266 3326 3992 4351 4510
Size: 648 843 598 659 60 666 359 159
Cuts at: 4510 518
Size: 2042
Fragments arranged by size:
2042 843 666 659 648 598 359 159
60
MspA1I CmG'CkG
Cuts at: 1606 2131 3450 4127 5275 5520 1606
Size: 525 1319 677 1148 245 2120
Fragments arranged by size:
2120 1319 1148 677 525 245
NaeI GCC'GGC
Cuts at: 2524 2524
Size: 6034
NcoI C'CATG_G
Cuts at: 513 1065 2004 3327 513
Size: 552 939 1323 3220
Fragments arranged by size:
3220 1323 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6034
NgoMIV G'CCGG_C
Cuts at: 2522 2522
Size: 6034
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6034
NotI GC'GGCC_GC
Cuts at: 3481 3481
Size: 6034
NspI r_CATG'y
Cuts at: 1132 1229 3205 3250 3392 4937 1132
Size: 97 1976 45 142 1545 2229
Fragments arranged by size:
2229 1976 1545 142 97 45
PasI CC'CwG_GG
Cuts at: 1348 2311 2626 1348
Size: 963 315 4756
Fragments arranged by size:
4756 963 315
PciI A'CATG_T
Cuts at: 3201 4933 3201
Size: 1732 4302
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2985 1108
Size: 1877 4157
PmeI GTTT'AAAC
Cuts at: 3338 3338
Size: 6034
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4103 4135 5616 5648 4103
Size: 32 1481 32 4489
Fragments arranged by size:
4489 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3007 3007
Size: 6034
PshAI GACnn'nnGTC
Cuts at: 1613 3202 1613
Size: 1589 4445
PsiI TTA'TAA
Cuts at: 3604 3604
Size: 6034
PspOMI G'GGCC_C
Cuts at: 2387 2387
Size: 6034
PstI C_TGCA'G
Cuts at: 830 2932 3386 830
Size: 2102 454 3478
Fragments arranged by size:
3478 2102 454
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6034
SalI G'TCGA_C
Cuts at: 1936 2644 3376 1936
Size: 708 732 4594
Fragments arranged by size:
4594 732 708
SanDI GG'GwC_CC
Cuts at: 3007 3007
Size: 6034
SbfI CC_TGCA'GG
Cuts at: 3386 3386
Size: 6034
ScaI AGT'ACT
Cuts at: 4282 5994 4282
Size: 1712 4322
SexAI A'CCwGG_T
Cuts at: 2562 2562
Size: 6034
SfcI C'TryA_G
Cuts at: 826 1045 2928 3382 4517 5198 5389 826
Size: 219 1883 454 1135 681 191 1471
Fragments arranged by size:
1883 1471 1135 681 454 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2528 2528
Size: 6034
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6034
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6034
SmaI CCC'GGG
Cuts at: 1872 2091 2385 3361 1872
Size: 219 294 976 4545
Fragments arranged by size:
4545 976 294 219
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2555 4138 5039 5301 820
Size: 197 926 612 1583 901 262 1553
Fragments arranged by size:
1583 1553 926 901 612 262 197
SnaBI TAC'GTA
Cuts at: 493 3825 493
Size: 3332 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6034
SphI G_CATG'C
Cuts at: 1132 3392 1132
Size: 2260 3774
SspI AAT'ATT
Cuts at: 5 52 3052 3958 5
Size: 47 3000 906 2081
Fragments arranged by size:
3000 2081 906 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6034
StyI C'CwwG_G
Cuts at: 513 1065 2004 2763 3327 3473 513
Size: 552 939 759 564 146 3074
Fragments arranged by size:
3074 939 759 564 552 146
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2023 2194 2618 3320 4061 4078 4231
Size: 1255 443 171 424 702 741 17 153
Cuts at: 4231 4416 4861 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 741 702 445 443 424 185
171 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2688 4280 5992
Size: 274 80 33 51 1443 711 1592 1712
Cuts at: 5992 96
Size: 138
Fragments arranged by size:
1712 1592 1443 711 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2315 2347 2363 2395 2938 2970 3191 3223 3263
Size: 32 16 32 543 32 221 32 40
Cuts at: 3263 3293 3295 3325 5994 6026 2315
Size: 30 2 30 2669 32 2323
Fragments arranged by size:
2669 2323 543 221 40 32 32 32
32 32 30 30 16 2
Tth111I GACn'n_nGTC
Cuts at: 1316 3011 1316
Size: 1695 4339
XbaI T'CTAG_A
Cuts at: 3370 3370
Size: 6034
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2719 2719
Size: 6034
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6034
XmaI C'CCGG_G
Cuts at: 1870 2089 2383 3359 1870
Size: 219 294 976 4545
Fragments arranged by size:
4545 976 294 219
XmnI GAAnn'nnTTC
Cuts at: 4163 4163
Size: 6034
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2364 2485 2949 3842
Size: 53 83 186 936 830 121 464 893
Cuts at: 3842 276
Size: 2468
Fragments arranged by size:
2468 936 893 830 464 186 121 83
53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI SalI SanDI
SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PfoI
PmlI PspXI PsrI PvuII RsrII SacI SacII SapI
SfoI SrfI SwaI