Restriction map for
pFN21AB8356
(Circular) (Six-base) MAPSORT of: pFN21AB8356.seq Check: 6025 from: 1 to: 5848
pFN21AB8356 5848 bp
With 182 enzymes: SgfI *
February 27, 2009 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2623 2623
Size: 5848
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3658 278
Size: 53 83 186 936 2122 2468
Fragments arranged by size:
2468 2122 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2571 3084 3191 1937
Size: 634 513 107 4594
Fragments arranged by size:
4594 634 513 107
Acc65I G'GTAC_C
Cuts at: 3169 3169
Size: 5848
AclI AA'CG_TT
Cuts at: 1563 3975 4348 1563
Size: 2412 373 3063
Fragments arranged by size:
3063 2412 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3911 5295 5563 1632
Size: 2279 1384 268 1917
Fragments arranged by size:
2279 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5651
AflIII A'CryG_T
Cuts at: 1203 2940 4747 5724 1203
Size: 1737 1807 977 1327
Fragments arranged by size:
1807 1737 1327 977
AgeI A'CCGG_T
Cuts at: 4679 4679
Size: 5848
AhdI GACnn_n'nnGTC
Cuts at: 4577 4577
Size: 5848
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5848
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3074 1184
Size: 1890 3958
AlwNI CAG_nnn'CTG
Cuts at: 3591 5163 3591
Size: 1572 4276
ApaI G_GGCC'C
Cuts at: 2039 2039
Size: 5848
ApaLI G'TGCA_C
Cuts at: 3905 5061 3905
Size: 1156 4692
ApoI r'AATT_y
Cuts at: 1493 3157 3383 3535 5644 1493
Size: 1664 226 152 2109 1697
Fragments arranged by size:
2109 1697 1664 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4402 160
Size: 872 3370 1606
Fragments arranged by size:
3370 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5848
AvaI C'yCGr_G
Cuts at: 1870 1943 2094 2615 3173 1870
Size: 73 151 521 558 4545
Fragments arranged by size:
4545 558 521 151 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3013 3046 3013
Size: 33 5815
BamHI G'GATC_C
Cuts at: 3178 3545 3178
Size: 367 5481
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3169 4624 618
Size: 325 217 627 1382 1455 1842
Fragments arranged by size:
1842 1455 1382 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2039 2550 3167 1391
Size: 648 511 617 4072
Fragments arranged by size:
4072 648 617 511
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4877
BbvCI CC'TCA_GC
Cuts at: 2384 2604 2910 2384
Size: 220 306 5322
Fragments arranged by size:
5322 306 220
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2387 2421 4039 4073 1903
Size: 34 450 34 1618 34 3678
Fragments arranged by size:
3678 1618 450 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3740 4950 3740
Size: 1210 4638
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5848
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2730 4459 136
Size: 107 122 71 631 605 1058 1729 1525
Fragments arranged by size:
1729 1525 1058 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3007 5843 3007
Size: 2836 3012
BlpI GC'TnA_GC
Cuts at: 3265 3265
Size: 5848
Bme1580I G_kGCm'C
Cuts at: 1790 2039 2239 3909 5065 1790
Size: 249 200 1670 1156 2573
Fragments arranged by size:
2573 1670 1156 249 200
BmgBI CAC'GTC
Cuts at: 1318 2374 1318
Size: 1056 4792
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2720 3579 4537
Size: 416 53 83 399 24 1303 859 958
Cuts at: 4537 442
Size: 1753
Fragments arranged by size:
1753 1303 958 859 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5848
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2545 4508 1239
Size: 1306 1963 2579
Fragments arranged by size:
2579 1963 1306
Bpu10I CC'TnA_GC
Cuts at: 2384 2604 2910 2384
Size: 220 306 5322
Fragments arranged by size:
5322 306 220
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2169 3128 3973 4838 5136 2169
Size: 959 845 865 298 2881
Fragments arranged by size:
2881 959 865 845 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4511 882
Size: 617 3012 2219
Fragments arranged by size:
3012 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3639 493
Size: 711 2435 2702
Fragments arranged by size:
2702 2435 711
BsaBI GATnn'nnATC
Cuts at: 5842 5842
Size: 5848
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3655 4037 4672 275
Size: 53 83 186 936 2122 382 635 1451
Fragments arranged by size:
2122 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2055 4281 4679 4953 5100 5511 2055
Size: 2226 398 274 147 411 2392
Fragments arranged by size:
2392 2226 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2358 2388 2476 2506 2619
Size: 30 165 30 955 30 88 30 113
Cuts at: 2619 2649 1178
Size: 30 4377
Fragments arranged by size:
4377 955 165 113 88 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2379 2497 2558 2572 2610 2621 2927 1186
Size: 1193 118 61 14 38 11 306 4107
Fragments arranged by size:
4107 1193 306 118 61 38 14 11
BseYI C'CCAG_C
Cuts at: 2038 5051 5547 2038
Size: 3013 496 2339
Fragments arranged by size:
3013 2339 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2651 2941 1163
Size: 573 915 290 4070
Fragments arranged by size:
4070 915 573 290
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3298 4059 5087 1540
Size: 275 186 1297 761 1028 2301
Fragments arranged by size:
2301 1297 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2919 3909 3994 5065 2919
Size: 990 85 1071 3702
Fragments arranged by size:
3702 1071 990 85
BsmI GAATG_Cn'
Cuts at: 3051 3359 3452 3051
Size: 308 93 5447
Fragments arranged by size:
5447 308 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2039 2239 2550 2919 3167 3909 3994
Size: 399 249 200 311 369 248 742 85
Cuts at: 3994 5065 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 369 311 249 248
200 85
BspEI T'CCGG_A
Cuts at: 2055 5511 2055
Size: 3456 2392
BspHI T'CATG_A
Cuts at: 3737 5467 3737
Size: 1730 4118
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2623 3203 844
Size: 1779 580 3489
Fragments arranged by size:
3489 1779 580
BsrBI CCG'CTC
Cuts at: 1394 1823 3735 1394
Size: 429 1912 3507
Fragments arranged by size:
3507 1912 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4343 4517 65
Size: 1183 3095 174 1396
Fragments arranged by size:
3095 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4492 4679 1700
Size: 110 2682 187 2869
Fragments arranged by size:
2869 2682 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5848
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5848
BssSI C'ACGA_G
Cuts at: 3908 4920 3908
Size: 1012 4836
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5848
BstBI TT'CG_AA
Cuts at: 4652 4652
Size: 5848
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5848
BstXI CCAn_nnnn'nTGG
Cuts at: 2341 2341
Size: 5848
BstYI r'GATC_y
Cuts at: 1972 2988 3007 3178 3545 3930 3947 5399 5485
Size: 1016 19 171 367 385 17 1452 86
Cuts at: 5485 5497 5843 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1016 385 367 346 171 86
19 17 12
Bsu36I CC'TnA_GG
Cuts at: 2301 2301
Size: 5848
BtgI C'CryG_G
Cuts at: 513 1065 2004 2464 513
Size: 552 939 460 3897
Fragments arranged by size:
3897 939 552 460
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5109
Fragments arranged by size:
5109 683 56
BtsI GCAGTG_nn'
Cuts at: 2114 2762 3364 4158 4178 2114
Size: 648 602 794 20 3784
Fragments arranged by size:
3784 794 648 602 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5848
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5813
DraI TTT'AAA
Cuts at: 3152 3152
Size: 5848
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4855 809
Size: 507 3539 1802
Fragments arranged by size:
3539 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2823 2863 3295
Size: 54 1425 50 275 18 993 40 432
Cuts at: 3295 4184 5813 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 993 889 432 275 54 50
43 40 18
EagI C'GGCC_G
Cuts at: 1537 1812 3295 1537
Size: 275 1483 4090
Fragments arranged by size:
4090 1483 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2799 3778 1353
Size: 1446 979 3423
Fragments arranged by size:
3423 1446 979
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4435 4809 4955 387
Size: 1064 138 2846 374 146 1280
Fragments arranged by size:
2846 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2545 3911 4508 5295 5563 1239
Size: 393 913 1366 597 787 268 1524
Fragments arranged by size:
1524 1366 913 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2035 2169 2035
Size: 134 5714
EcoRI G'AATT_C
Cuts at: 3157 3535 3157
Size: 378 5470
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2677 2677
Size: 5848
FspI TGC'GCA
Cuts at: 4354 4354
Size: 5848
HaeII r_GCGC'y
Cuts at: 1678 2052 2659 4995 1678
Size: 374 607 2336 2531
Fragments arranged by size:
2531 2336 607 374
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4489 4521 4563 4595 1373
Size: 32 135 32 2917 32 42 32 2626
Fragments arranged by size:
2917 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3192 3438 669
Size: 988 281 1254 246 3079
Fragments arranged by size:
3079 1254 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5848
HpaI GTT'AAC
Cuts at: 3438 3438
Size: 5848
KpnI G_GTAC'C
Cuts at: 3173 3173
Size: 5848
MfeI C'AATT_G
Cuts at: 3447 3447
Size: 5848
MluI A'CGCG_T
Cuts at: 5724 5724
Size: 5848
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4962 5146 1305
Size: 3657 184 2007
Fragments arranged by size:
3657 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2825 10
Size: 54 1425 343 993 3033
Fragments arranged by size:
3033 1425 993 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2274 3056 3806 4165 4324 518
Size: 648 1108 782 750 359 159 2042
Fragments arranged by size:
2042 1108 782 750 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2586 3264 3941 5089 5334 1606
Size: 980 678 677 1148 245 2120
Fragments arranged by size:
2120 1148 980 678 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4357
Fragments arranged by size:
4357 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5848
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5848
NotI GC'GGCC_GC
Cuts at: 3295 3295
Size: 5848
NspI r_CATG'y
Cuts at: 1132 1229 2944 3206 4751 1132
Size: 97 1715 262 1545 2229
Fragments arranged by size:
2229 1715 1545 262 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5848
PciI A'CATG_T
Cuts at: 2940 4747 2940
Size: 1807 4041
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2523 1108
Size: 1415 4433
PmeI GTTT'AAAC
Cuts at: 3152 3152
Size: 5848
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3917 3949 5430 5462 3917
Size: 32 1481 32 4303
Fragments arranged by size:
4303 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2169 2169
Size: 5848
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5848
PsiI TTA'TAA
Cuts at: 3418 3418
Size: 5848
PspOMI G'GGCC_C
Cuts at: 2035 2035
Size: 5848
PstI C_TGCA'G
Cuts at: 830 3200 830
Size: 2370 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5848
SalI G'TCGA_C
Cuts at: 1936 3190 1936
Size: 1254 4594
SanDI GG'GwC_CC
Cuts at: 2169 2169
Size: 5848
SbfI CC_TGCA'GG
Cuts at: 3200 3200
Size: 5848
ScaI AGT'ACT
Cuts at: 4096 5808 4096
Size: 1712 4136
SexAI A'CCwGG_T
Cuts at: 2499 2499
Size: 5848
SfcI C'TryA_G
Cuts at: 826 1045 2148 2923 3196 4331 5012 5203 826
Size: 219 1103 775 273 1135 681 191 1471
Fragments arranged by size:
1471 1135 1103 775 681 273 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5848
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5848
SmaI CCC'GGG
Cuts at: 1872 3175 1872
Size: 1303 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2184 3143 3952 4853 5115 820
Size: 197 926 241 959 809 901 262 1553
Fragments arranged by size:
1553 959 926 901 809 262 241 197
SnaBI TAC'GTA
Cuts at: 493 3639 493
Size: 3146 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5848
SphI G_CATG'C
Cuts at: 1132 3206 1132
Size: 2074 3774
SspI AAT'ATT
Cuts at: 5 52 3772 5
Size: 47 3720 2081
Fragments arranged by size:
3720 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5848
StyI C'CwwG_G
Cuts at: 513 1065 2004 2517 3287 513
Size: 552 939 513 770 3074
Fragments arranged by size:
3074 939 770 552 513
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3875 3892 4045 4230 4675 325
Size: 1255 2295 17 153 185 445 1498
Fragments arranged by size:
2295 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4094 5806 96
Size: 274 80 33 51 1443 2117 1712 138
Fragments arranged by size:
2117 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2358 2390 2476 2508 2619 2651 2762 2794 5808
Size: 32 86 32 111 32 111 32 3014
Cuts at: 5808 5840 2358
Size: 32 2366
Fragments arranged by size:
3014 2366 111 111 86 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5848
XbaI T'CTAG_A
Cuts at: 3184 3184
Size: 5848
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2978 2999 2978
Size: 21 5827
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5848
XmaI C'CCGG_G
Cuts at: 1870 3173 1870
Size: 1303 4545
XmnI GAAnn'nnTTC
Cuts at: 3977 3977
Size: 5848
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3656 276
Size: 53 83 186 936 2122 2468
Fragments arranged by size:
2468 2122 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI SalI SanDI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BfrBI BplI BsiWI
BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PspXI PsrI PvuII RsrII SacI SacII SapI
SfiI SfoI SrfI SwaI