Restriction map for
pFN21AB8348
(Circular) (Six-base) MAPSORT of: pFN21AB8348.seq Check: 7061 from: 1 to: 6142
pFN21AB8348 6142 bp
With 182 enzymes: SgfI *
February 27, 2009 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2469 3952 278
Size: 53 83 186 936 933 1483 2468
Fragments arranged by size:
2468 1483 936 933 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3485 1937
Size: 1548 4594
Acc65I G'GTAC_C
Cuts at: 3463 3463
Size: 6142
AclI AA'CG_TT
Cuts at: 1563 4269 4642 1563
Size: 2706 373 3063
Fragments arranged by size:
3063 2706 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2601 2691 2739 2874 4205 5589 5857 1632
Size: 969 90 48 135 1331 1384 268 1917
Fragments arranged by size:
1917 1384 1331 969 268 135 90 48
AfeI AGC'GCT
Cuts at: 3158 3158
Size: 6142
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5945
AflIII A'CryG_T
Cuts at: 1203 2152 5041 6018 1203
Size: 949 2889 977 1327
Fragments arranged by size:
2889 1327 977 949
AgeI A'CCGG_T
Cuts at: 2786 4973 2786
Size: 2187 3955
AhdI GACnn_n'nnGTC
Cuts at: 4871 4871
Size: 6142
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6142
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6142
AlwNI CAG_nnn'CTG
Cuts at: 2431 3885 5457 2431
Size: 1454 1572 3116
Fragments arranged by size:
3116 1572 1454
ApaLI G'TGCA_C
Cuts at: 4199 5355 4199
Size: 1156 4986
ApoI r'AATT_y
Cuts at: 1493 3364 3451 3677 3829 5938 1493
Size: 1871 87 226 152 2109 1697
Fragments arranged by size:
2109 1871 1697 226 152 87
AseI AT'TA_AT
Cuts at: 160 1032 4696 160
Size: 872 3664 1606
Fragments arranged by size:
3664 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6142
AvaI C'yCGr_G
Cuts at: 1870 1943 3467 1870
Size: 73 1524 4545
Fragments arranged by size:
4545 1524 73
BamHI G'GATC_C
Cuts at: 3031 3472 3839 3031
Size: 441 367 5334
Fragments arranged by size:
5334 441 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3005 3298 3463 4918 618
Size: 325 217 627 1218 293 165 1455 1842
Fragments arranged by size:
1842 1455 1218 627 325 293 217 165
BanII G_rGCy'C
Cuts at: 1391 2150 2766 2964 3263 3461 1391
Size: 759 616 198 299 198 4072
Fragments arranged by size:
4072 759 616 299 198 198
BbeI G_GCGC'C
Cuts at: 3302 3302
Size: 6142
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2862 928
Size: 971 963 4208
Fragments arranged by size:
4208 971 963
BbvCI CC'TCA_GC
Cuts at: 2160 2160
Size: 6142
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2362 2396 4333 4367 1903
Size: 34 425 34 1937 34 3678
Fragments arranged by size:
3678 1937 425 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4034 5244 4034
Size: 1210 4932
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6142
BfrBI ATG'CAT
Cuts at: 2662 2662
Size: 6142
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4753 136
Size: 107 122 71 631 605 3081 1525
Fragments arranged by size:
3081 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6137 6137
Size: 6142
BlpI GC'TnA_GC
Cuts at: 3098 3559 3098
Size: 461 5681
Bme1580I G_kGCm'C
Cuts at: 1790 2847 3434 4203 5359 1790
Size: 1057 587 769 1156 2573
Fragments arranged by size:
2573 1156 1057 769 587
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6142
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2803 3384 3873
Size: 416 53 83 399 24 1386 581 489
Cuts at: 3873 4831 442
Size: 958 1753
Fragments arranged by size:
1753 1386 958 581 489 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6142
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2101 2453 2968 2978 3185 3294 4802 1239
Size: 862 352 515 10 207 109 1508 2579
Fragments arranged by size:
2579 1508 862 515 352 207 109 10
Bpu10I CC'TnA_GC
Cuts at: 2160 2160
Size: 6142
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2221 4267 5132 5430 2221
Size: 2046 865 298 2933
Fragments arranged by size:
2933 2046 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4805 882
Size: 617 3306 2219
Fragments arranged by size:
3306 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3933 493
Size: 711 2729 2702
Fragments arranged by size:
2729 2702 711
BsaBI GATnn'nnATC
Cuts at: 2880 6136 2880
Size: 3256 2886
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2466 3299 3949 4331
Size: 53 83 186 936 933 833 650 382
Cuts at: 4331 4966 275
Size: 635 1451
Fragments arranged by size:
1451 936 933 833 650 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2786 4575 4973 5247 5394 5805 2786
Size: 1789 398 274 147 411 3123
Fragments arranged by size:
3123 1789 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2951 2981 3245 3275 1178
Size: 30 165 30 1548 30 264 30 4045
Fragments arranged by size:
4045 1548 264 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2104 2345 2589 1186
Size: 918 241 244 4739
Fragments arranged by size:
4739 918 244 241
BseYI C'CCAG_C
Cuts at: 2185 2546 3054 5345 5841 2185
Size: 361 508 2291 496 2486
Fragments arranged by size:
2486 2291 508 496 361
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2703 1163
Size: 573 967 4602
Fragments arranged by size:
4602 967 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3592 4353 5381 1540
Size: 275 186 1591 761 1028 2301
Fragments arranged by size:
2301 1591 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2964 4203 4288 5359 2964
Size: 1239 85 1071 3747
Fragments arranged by size:
3747 1239 1071 85
BsmI GAATG_Cn'
Cuts at: 2261 3653 3746 2261
Size: 1392 93 4657
Fragments arranged by size:
4657 1392 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2150 2766 2847 2964 3263 3434 3461
Size: 399 360 616 81 117 299 171 27
Cuts at: 3461 4203 4288 5359 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 616 399 360 299 171
117 85 81 27
BspEI T'CCGG_A
Cuts at: 5805 5805
Size: 6142
BspHI T'CATG_A
Cuts at: 3135 4031 5761 3135
Size: 896 1730 3516
Fragments arranged by size:
3516 1730 896
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3497 844
Size: 2653 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3064 4029 1394
Size: 429 1241 965 3507
Fragments arranged by size:
3507 1241 965 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4637 4811 65
Size: 1183 3389 174 1396
Fragments arranged by size:
3389 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2786 4786 4973 1700
Size: 110 976 2000 187 2869
Fragments arranged by size:
2869 2000 976 187 110
BsrGI T'GTAC_A
Cuts at: 96 3228 96
Size: 3132 3010
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6142
BssSI C'ACGA_G
Cuts at: 4202 5214 4202
Size: 1012 5130
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6142
BstBI TT'CG_AA
Cuts at: 4946 4946
Size: 6142
BstEII G'GTnAC_C
Cuts at: 1184 2194 1184
Size: 1010 5132
BstXI CCAn_nnnn'nTGG
Cuts at: 2206 2231 2206
Size: 25 6117
BstYI r'GATC_y
Cuts at: 1972 2392 2875 2934 3031 3472 3839 4224 4241
Size: 420 483 59 97 441 367 385 17
Cuts at: 4241 5693 5779 5791 6137 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 483 441 420 385 367 346
97 86 59 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2651 3148 3181 513
Size: 552 939 647 497 33 3474
Fragments arranged by size:
3474 939 647 552 497 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5403
Fragments arranged by size:
5403 683 56
BtsI GCAGTG_nn'
Cuts at: 2266 2432 2932 3658 4452 4472 2266
Size: 166 500 726 794 20 3936
Fragments arranged by size:
3936 794 726 500 166 20
ClaI AT'CG_AT
Cuts at: 1554 2493 1554
Size: 939 5203
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6107
DraI TTT'AAA
Cuts at: 3446 3446
Size: 6142
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5149 809
Size: 507 3833 1802
Fragments arranged by size:
3833 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2265 2794
Size: 54 1425 50 275 18 177 258 529
Cuts at: 2794 2929 3589 4478 6107 8
Size: 135 660 889 1629 43
Fragments arranged by size:
1629 1425 889 660 529 275 258 177
135 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3589 1537
Size: 275 1777 4090
Fragments arranged by size:
4090 1777 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2607 3062 4072 1353
Size: 1254 455 1010 3423
Fragments arranged by size:
3423 1254 1010 455
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4729 5103 5249 387
Size: 1064 138 3140 374 146 1280
Fragments arranged by size:
3140 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2962 2962
Size: 6142
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2101 2453 2601 2691 2739 2874 2968
Size: 393 469 352 148 90 48 135 94
Cuts at: 2968 2978 3185 3294 4205 4802 5589 5857 1239
Size: 10 207 109 911 597 787 268 1524
Fragments arranged by size:
1524 911 787 597 469 393 352 268
207 148 135 109 94 90 48 10
EcoO109I rG'GnC_Cy
Cuts at: 3196 3196
Size: 6142
EcoRI G'AATT_C
Cuts at: 3364 3451 3829 3364
Size: 87 378 5677
Fragments arranged by size:
5677 378 87
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2044 2545 3193 3307 2044
Size: 501 648 114 4879
Fragments arranged by size:
4879 648 501 114
FspI TGC'GCA
Cuts at: 4648 4648
Size: 6142
HaeII r_GCGC'y
Cuts at: 1678 3055 3160 3302 5289 1678
Size: 1377 105 142 1987 2531
Fragments arranged by size:
2531 1987 1377 142 105
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2036 2068 2159 2191 2951
Size: 32 135 32 464 32 91 32 760
Cuts at: 2951 2983 3016 3048 4783 4815 4857 4889 1373
Size: 32 33 32 1735 32 42 32 2626
Fragments arranged by size:
2626 1735 760 464 135 91 42 33
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2470 3486 3732 669
Size: 988 281 532 1016 246 3079
Fragments arranged by size:
3079 1016 988 532 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6142
HpaI GTT'AAC
Cuts at: 3732 3732
Size: 6142
KasI G'GCGC_C
Cuts at: 3298 3298
Size: 6142
KpnI G_GTAC'C
Cuts at: 3467 3467
Size: 6142
MfeI C'AATT_G
Cuts at: 3741 3741
Size: 6142
MluI A'CGCG_T
Cuts at: 2152 6018 2152
Size: 3866 2276
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2949 3003 5256 5440 1305
Size: 1644 54 2253 184 2007
Fragments arranged by size:
2253 2007 1644 184 54
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2267 2931 10
Size: 54 1425 343 177 258 664 3221
Fragments arranged by size:
3221 1425 664 343 258 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2204 4100 4459 4618 518
Size: 648 1038 1896 359 159 2042
Fragments arranged by size:
2042 1896 1038 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2988 3558 4235 5383 5628 1606
Size: 1382 570 677 1148 245 2120
Fragments arranged by size:
2120 1382 1148 677 570 245
NarI GG'CG_CC
Cuts at: 3299 3299
Size: 6142
NcoI C'CATG_G
Cuts at: 513 1065 2004 3148 513
Size: 552 939 1144 3507
Fragments arranged by size:
3507 1144 939 552
NdeI CA'TA_TG
Cuts at: 387 2758 3414 387
Size: 2371 656 3115
Fragments arranged by size:
3115 2371 656
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6142
NotI GC'GGCC_GC
Cuts at: 3589 3589
Size: 6142
NsiI A_TGCA'T
Cuts at: 2664 2664
Size: 6142
NspI r_CATG'y
Cuts at: 1132 1229 2344 2666 3500 5045 1132
Size: 97 1115 322 834 1545 2229
Fragments arranged by size:
2229 1545 1115 834 322 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6142
PciI A'CATG_T
Cuts at: 5041 5041
Size: 6142
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2927 1108
Size: 1819 4323
PfoI T'CCnGG_A
Cuts at: 2955 2955
Size: 6142
PmeI GTTT'AAAC
Cuts at: 3446 3446
Size: 6142
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4211 4243 5724 5756 4211
Size: 32 1481 32 4597
Fragments arranged by size:
4597 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2862 3143 1613
Size: 1249 281 4612
Fragments arranged by size:
4612 1249 281
PsiI TTA'TAA
Cuts at: 3712 3712
Size: 6142
PstI C_TGCA'G
Cuts at: 830 3494 830
Size: 2664 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6142
PvuII CAG'CTG
Cuts at: 2988 2988
Size: 6142
SacI G_AGCT'C
Cuts at: 2964 2964
Size: 6142
SalI G'TCGA_C
Cuts at: 1936 3484 1936
Size: 1548 4594
SapI GCTCTTCn'nnn_
Cuts at: 3062 3062
Size: 6142
SbfI CC_TGCA'GG
Cuts at: 3494 3494
Size: 6142
ScaI AGT'ACT
Cuts at: 4390 6102 4390
Size: 1712 4430
SfcI C'TryA_G
Cuts at: 826 1045 3490 4625 5306 5497 826
Size: 219 2445 1135 681 191 1471
Fragments arranged by size:
2445 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 3300 3300
Size: 6142
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6142
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6142
SmaI CCC'GGG
Cuts at: 1872 3469 1872
Size: 1597 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2236 4246 5147 5409 820
Size: 197 926 293 2010 901 262 1553
Fragments arranged by size:
2010 1553 926 901 293 262 197
SnaBI TAC'GTA
Cuts at: 493 3933 493
Size: 3440 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6142
SphI G_CATG'C
Cuts at: 1132 2666 3500 1132
Size: 1534 834 3774
Fragments arranged by size:
3774 1534 834
SspI AAT'ATT
Cuts at: 5 52 4066 5
Size: 47 4014 2081
Fragments arranged by size:
4014 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6142
StyI C'CwwG_G
Cuts at: 513 1065 2004 2520 3141 3148 3581 513
Size: 552 939 516 621 7 433 3074
Fragments arranged by size:
3074 939 621 552 516 433 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3291 4169 4186 4339 4524 4969 325
Size: 1255 1711 878 17 153 185 445 1498
Fragments arranged by size:
1711 1498 1255 878 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2408 3228 4388
Size: 274 80 33 51 1443 431 820 1160
Cuts at: 4388 6100 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1160 820 431 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6102 6134 6102
Size: 32 6110
Tth111I GACn'n_nGTC
Cuts at: 1316 2107 1316
Size: 791 5351
XbaI T'CTAG_A
Cuts at: 3478 3478
Size: 6142
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2492 3357 2492
Size: 865 5277
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6142
XmaI C'CCGG_G
Cuts at: 1870 3467 1870
Size: 1597 4545
XmnI GAAnn'nnTTC
Cuts at: 4271 4271
Size: 6142
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2467 3950 276
Size: 53 83 186 936 933 1483 2468
Fragments arranged by size:
2468 1483 936 933 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI
NaeI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI