Restriction map for
pFN21AB8347
(Circular) (Six-base) MAPSORT of: pFN21AB8347.seq Check: 9606 from: 1 to: 5839
pFN21AB8347 5839 bp
With 182 enzymes: SgfI *
February 27, 2009 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2617 2617
Size: 5839
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3649 278
Size: 53 83 186 936 2113 2468
Fragments arranged by size:
2468 2113 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2895 3182 1937
Size: 958 287 4594
Fragments arranged by size:
4594 958 287
Acc65I G'GTAC_C
Cuts at: 3160 3160
Size: 5839
AclI AA'CG_TT
Cuts at: 1563 3966 4339 1563
Size: 2403 373 3063
Fragments arranged by size:
3063 2403 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2228 2359 3902 5286 5554 1632
Size: 596 131 1543 1384 268 1917
Fragments arranged by size:
1917 1543 1384 596 268 131
AfeI AGC'GCT
Cuts at: 3008 3008
Size: 5839
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5642
AflIII A'CryG_T
Cuts at: 1203 4738 5715 1203
Size: 3535 977 1327
Fragments arranged by size:
3535 1327 977
AgeI A'CCGG_T
Cuts at: 4670 4670
Size: 5839
AhdI GACnn_n'nnGTC
Cuts at: 4568 4568
Size: 5839
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5839
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5839
AlwNI CAG_nnn'CTG
Cuts at: 2163 2275 2308 2953 3080 3582 5154 2163
Size: 112 33 645 127 502 1572 2848
Fragments arranged by size:
2848 1572 645 502 127 112 33
ApaLI G'TGCA_C
Cuts at: 2613 3896 5052 2613
Size: 1283 1156 3400
Fragments arranged by size:
3400 1283 1156
ApoI r'AATT_y
Cuts at: 1493 3148 3374 3526 5635 1493
Size: 1655 226 152 2109 1697
Fragments arranged by size:
2109 1697 1655 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4393 160
Size: 872 3361 1606
Fragments arranged by size:
3361 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5839
AvaI C'yCGr_G
Cuts at: 1870 1943 2109 2402 2694 3164 1870
Size: 73 166 293 292 470 4545
Fragments arranged by size:
4545 470 293 292 166 73
BamHI G'GATC_C
Cuts at: 3169 3536 3169
Size: 367 5472
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3160 4615 618
Size: 325 217 627 1373 1455 1842
Fragments arranged by size:
1842 1455 1373 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3158 1391
Size: 1767 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2058 2414 928
Size: 971 159 356 4353
Fragments arranged by size:
4353 971 356 159
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4030 4064 1903
Size: 34 2093 34 3678
Fragments arranged by size:
3678 2093 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3731 4941 3731
Size: 1210 4629
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5839
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2874 3053 4450
Size: 107 122 71 631 605 1202 179 1397
Cuts at: 4450 136
Size: 1525
Fragments arranged by size:
1525 1397 1202 631 605 179 122 107
71
BglII A'GATC_T
Cuts at: 5834 5834
Size: 5839
BlpI GC'TnA_GC
Cuts at: 2360 3256 2360
Size: 896 4943
Bme1580I G_kGCm'C
Cuts at: 1790 2617 3900 5056 1790
Size: 827 1283 1156 2573
Fragments arranged by size:
2573 1283 1156 827
BmgBI CAC'GTC
Cuts at: 1318 2944 1318
Size: 1626 4213
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3570 4528 442
Size: 416 53 83 399 24 2153 958 1753
Fragments arranged by size:
2153 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5839
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2437 2437
Size: 5839
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2237 2267 2330 2684 2903 4499 1239
Size: 998 30 63 354 219 1596 2579
Fragments arranged by size:
2579 1596 998 354 219 63 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3964 4829 5127 3964
Size: 865 298 4676
Fragments arranged by size:
4676 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4502 882
Size: 617 3003 2219
Fragments arranged by size:
3003 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3630 493
Size: 711 2426 2702
Fragments arranged by size:
2702 2426 711
BsaBI GATnn'nnATC
Cuts at: 5833 5833
Size: 5839
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2961 3646 4028 4663
Size: 53 83 186 936 1428 685 382 635
Cuts at: 4663 275
Size: 1451
Fragments arranged by size:
1451 1428 936 685 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4272 4670 4944 5091 5502 4272
Size: 398 274 147 411 4609
Fragments arranged by size:
4609 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5614
Fragments arranged by size:
5614 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2300 2327 2429 2441 2570 2618 2855 2858
Size: 1114 27 102 12 129 48 237 3
Cuts at: 2858 2861 3083 1186
Size: 3 222 3942
Fragments arranged by size:
3942 1114 237 222 129 102 48 27
12 3 3
BseYI C'CCAG_C
Cuts at: 2295 2505 5042 5538 2295
Size: 210 2537 496 2596
Fragments arranged by size:
2596 2537 496 210
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2966 1163
Size: 573 1230 4036
Fragments arranged by size:
4036 1230 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2740 3289 4050 5078 1540
Size: 275 186 739 549 761 1028 2301
Fragments arranged by size:
2301 1028 761 739 549 275 186
BsiHKAI G_wGCw'C
Cuts at: 2617 3900 3985 5056 2617
Size: 1283 85 1071 3400
Fragments arranged by size:
3400 1283 1071 85
BsmI GAATG_Cn'
Cuts at: 3350 3443 3350
Size: 93 5746
BsmBI CGTCTCn'nnnn_
Cuts at: 2051 2051
Size: 5839
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2617 3158 3900 3985 5056 1391
Size: 399 827 541 742 85 1071 2174
Fragments arranged by size:
2174 1071 827 742 541 399 85
BspEI T'CCGG_A
Cuts at: 5502 5502
Size: 5839
BspHI T'CATG_A
Cuts at: 3728 5458 3728
Size: 1730 4109
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2617 3065 3194 844
Size: 1773 448 129 3489
Fragments arranged by size:
3489 1773 448 129
BsrBI CCG'CTC
Cuts at: 1394 1823 2290 3726 1394
Size: 429 467 1436 3507
Fragments arranged by size:
3507 1436 467 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4334 4508 65
Size: 1183 3086 174 1396
Fragments arranged by size:
3086 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4483 4670 1700
Size: 110 2673 187 2869
Fragments arranged by size:
2869 2673 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5839
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5839
BssSI C'ACGA_G
Cuts at: 3899 4911 3899
Size: 1012 4827
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2953 794
Size: 2159 3680
BstBI TT'CG_AA
Cuts at: 4643 4643
Size: 5839
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5839
BstXI CCAn_nnnn'nTGG
Cuts at: 2572 2572
Size: 5839
BstYI r'GATC_y
Cuts at: 1972 3169 3536 3921 3938 5390 5476 5488 5834
Size: 1197 367 385 17 1452 86 12 346
Cuts at: 5834 1972
Size: 1977
Fragments arranged by size:
1977 1452 1197 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2910 2910
Size: 5839
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4348
Fragments arranged by size:
4348 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3132 487
Size: 683 56 1906 3194
Fragments arranged by size:
3194 1906 683 56
BtsI GCAGTG_nn'
Cuts at: 3355 4149 4169 3355
Size: 794 20 5025
Fragments arranged by size:
5025 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5839
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5804
DraI TTT'AAA
Cuts at: 3143 3143
Size: 5839
DraIII CAC_nnn'GTG
Cuts at: 2548 2548
Size: 5839
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4846 809
Size: 507 3530 1802
Fragments arranged by size:
3530 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2079 2187 2223
Size: 54 1425 50 275 18 249 108 36
Cuts at: 2223 2670 3286 4175 5804 8
Size: 447 616 889 1629 43
Fragments arranged by size:
1629 1425 889 616 447 275 249 108
54 50 43 36 18
EagI C'GGCC_G
Cuts at: 1537 1812 3286 1537
Size: 275 1474 4090
Fragments arranged by size:
4090 1474 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2093 2327 3769 1353
Size: 740 234 1442 3423
Fragments arranged by size:
3423 1442 740 234
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4426 4800 4946 387
Size: 1064 138 2837 374 146 1280
Fragments arranged by size:
2837 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2228 2237 2267 2330 2359 2684 2903
Size: 393 596 9 30 63 29 325 219
Cuts at: 2903 3902 4499 5286 5554 1239
Size: 999 597 787 268 1524
Fragments arranged by size:
1524 999 787 597 596 393 325 268
219 63 30 29 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2909 2909
Size: 5839
EcoO109I rG'GnC_Cy
Cuts at: 3031 3077 3085 3031
Size: 46 8 5785
Fragments arranged by size:
5785 46 8
EcoRI G'AATT_C
Cuts at: 3148 3526 3148
Size: 378 5461
FspI TGC'GCA
Cuts at: 2279 2321 2813 4345 2279
Size: 42 492 1532 3773
Fragments arranged by size:
3773 1532 492 42
HaeII r_GCGC'y
Cuts at: 1678 2254 3010 4986 1678
Size: 576 756 1976 2531
Fragments arranged by size:
2531 1976 756 576
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2722 2754 4480 4512 4554
Size: 32 135 32 1150 32 1726 32 42
Cuts at: 4554 4586 1373
Size: 32 2626
Fragments arranged by size:
2626 1726 1150 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3183 3429 669
Size: 988 281 1245 246 3079
Fragments arranged by size:
3079 1245 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5839
HpaI GTT'AAC
Cuts at: 3429 3429
Size: 5839
KpnI G_GTAC'C
Cuts at: 3164 3164
Size: 5839
MfeI C'AATT_G
Cuts at: 3438 3438
Size: 5839
MluI A'CGCG_T
Cuts at: 5715 5715
Size: 5839
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4953 5137 1305
Size: 3648 184 2007
Fragments arranged by size:
3648 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2672 10
Size: 54 1425 343 840 3177
Fragments arranged by size:
3177 1425 840 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2036 3797 4156 4315 518
Size: 648 870 1761 359 159 2042
Fragments arranged by size:
2042 1761 870 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2094 2275 2505 3255 3932 5080 5325 1606
Size: 488 181 230 750 677 1148 245 2120
Fragments arranged by size:
2120 1148 750 677 488 245 230 181
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4348
Fragments arranged by size:
4348 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5839
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5839
NotI GC'GGCC_GC
Cuts at: 3286 3286
Size: 5839
NspI r_CATG'y
Cuts at: 1132 1229 2341 2497 3004 3197 4742 1132
Size: 97 1112 156 507 193 1545 2229
Fragments arranged by size:
2229 1545 1112 507 193 156 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5839
PciI A'CATG_T
Cuts at: 4738 4738
Size: 5839
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2749 1108
Size: 1641 4198
PfoI T'CCnGG_A
Cuts at: 2661 2661
Size: 5839
PmeI GTTT'AAAC
Cuts at: 3143 3143
Size: 5839
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3908 3940 5421 5453 3908
Size: 32 1481 32 4294
Fragments arranged by size:
4294 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3031 3077 3085 3031
Size: 46 8 5785
Fragments arranged by size:
5785 46 8
PshAI GACnn'nnGTC
Cuts at: 1613 2645 1613
Size: 1032 4807
PsiI TTA'TAA
Cuts at: 3409 3409
Size: 5839
PstI C_TGCA'G
Cuts at: 830 2958 3191 830
Size: 2128 233 3478
Fragments arranged by size:
3478 2128 233
PvuI CG_AT'CG
Cuts at: 2001 2740 2001
Size: 739 5100
PvuII CAG'CTG
Cuts at: 2275 2275
Size: 5839
SalI G'TCGA_C
Cuts at: 1936 3181 1936
Size: 1245 4594
SanDI GG'GwC_CC
Cuts at: 3031 3085 3031
Size: 54 5785
SapI GCTCTTCn'nnn_
Cuts at: 2327 2327
Size: 5839
SbfI CC_TGCA'GG
Cuts at: 3191 3191
Size: 5839
ScaI AGT'ACT
Cuts at: 4087 5799 4087
Size: 1712 4127
SexAI A'CCwGG_T
Cuts at: 2544 2571 2544
Size: 27 5812
SfcI C'TryA_G
Cuts at: 826 1045 2014 2954 3187 4322 5003 5194 826
Size: 219 969 940 233 1135 681 191 1471
Fragments arranged by size:
1471 1135 969 940 681 233 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2874 3053 2874
Size: 179 5660
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5839
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5839
SmaI CCC'GGG
Cuts at: 1872 2111 2696 3166 1872
Size: 239 585 470 4545
Fragments arranged by size:
4545 585 470 239
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3943 4844 5106 820
Size: 197 926 2000 901 262 1553
Fragments arranged by size:
2000 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3630 493
Size: 3137 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5839
SphI G_CATG'C
Cuts at: 1132 2341 2497 3197 1132
Size: 1209 156 700 3774
Fragments arranged by size:
3774 1209 700 156
SspI AAT'ATT
Cuts at: 5 52 3763 5
Size: 47 3711 2081
Fragments arranged by size:
3711 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5839
StyI C'CwwG_G
Cuts at: 513 1065 2004 3057 3278 513
Size: 552 939 1053 221 3074
Fragments arranged by size:
3074 1053 939 552 221
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3866 3883 4036 4221 4666 325
Size: 1255 2286 17 153 185 445 1498
Fragments arranged by size:
2286 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2745 4085 5797
Size: 274 80 33 51 1443 768 1340 1712
Cuts at: 5797 96
Size: 138
Fragments arranged by size:
1712 1443 1340 768 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5799 5831 5799
Size: 32 5807
Tth111I GACn'n_nGTC
Cuts at: 1316 2537 2885 1316
Size: 1221 348 4270
Fragments arranged by size:
4270 1221 348
XbaI T'CTAG_A
Cuts at: 3175 3175
Size: 5839
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2971 2971
Size: 5839
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5839
XmaI C'CCGG_G
Cuts at: 1870 2109 2694 3164 1870
Size: 239 585 470 4545
Fragments arranged by size:
4545 585 470 239
XmnI GAAnn'nnTTC
Cuts at: 2927 3102 3968 2927
Size: 175 866 4798
Fragments arranged by size:
4798 866 175
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3647 276
Size: 53 83 186 936 2113 2468
Fragments arranged by size:
2468 2113 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BstZ17I EcoICRI EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspOMI PspXI PsrI RsrII SacI SacII SfoI SrfI
SwaI