Restriction map for
pFN21AB8345
(Circular) (Six-base) MAPSORT of: pFN21AB8345.seq Check: 4063 from: 1 to: 6589
pFN21AB8345 6589 bp
With 182 enzymes: SgfI *
February 27, 2009 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4399 278
Size: 53 83 186 936 2863 2468
Fragments arranged by size:
2863 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3932 1937
Size: 1995 4594
Acc65I G'GTAC_C
Cuts at: 3910 3910
Size: 6589
AclI AA'CG_TT
Cuts at: 1563 2813 4716 5089 1563
Size: 1250 1903 373 3063
Fragments arranged by size:
3063 1903 1250 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2950 3370 3450 3813 4652 6036 6304 1632
Size: 1318 420 80 363 839 1384 268 1917
Fragments arranged by size:
1917 1384 1318 839 420 363 268 80
AflII C'TTAA_G
Cuts at: 820 1017 3739 820
Size: 197 2722 3670
Fragments arranged by size:
3670 2722 197
AflIII A'CryG_T
Cuts at: 1203 2442 3412 3580 3748 5488 6465 1203
Size: 1239 970 168 168 1740 977 1327
Fragments arranged by size:
1740 1327 1239 977 970 168 168
AgeI A'CCGG_T
Cuts at: 5420 5420
Size: 6589
AhdI GACnn_n'nnGTC
Cuts at: 5318 5318
Size: 6589
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6589
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6589
AlwNI CAG_nnn'CTG
Cuts at: 2122 4332 5904 2122
Size: 2210 1572 2807
Fragments arranged by size:
2807 2210 1572
ApaLI G'TGCA_C
Cuts at: 3254 4646 5802 3254
Size: 1392 1156 4041
Fragments arranged by size:
4041 1392 1156
ApoI r'AATT_y
Cuts at: 1493 2262 2409 3812 3898 4124 4276 6385 1493
Size: 769 147 1403 86 226 152 2109 1697
Fragments arranged by size:
2109 1697 1403 769 226 152 147 86
AseI AT'TA_AT
Cuts at: 160 1032 5143 160
Size: 872 4111 1606
Fragments arranged by size:
4111 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6589
AvaI C'yCGr_G
Cuts at: 1870 1943 3914 1870
Size: 73 1971 4545
Fragments arranged by size:
4545 1971 73
BamHI G'GATC_C
Cuts at: 3919 4286 3919
Size: 367 6222
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3910 5365 618
Size: 325 217 627 2123 1455 1842
Fragments arranged by size:
2123 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3908 1391
Size: 2517 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2449 2827 2911 2995 3079 3134 3163
Size: 971 550 378 84 84 84 55 29
Cuts at: 3163 3438 3628 928
Size: 275 190 3889
Fragments arranged by size:
3889 971 550 378 275 190 84 84
84 55 29
BbvCI CC'TCA_GC
Cuts at: 2375 2375
Size: 6589
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4780 4814 1903
Size: 34 2843 34 3678
Fragments arranged by size:
3678 2843 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4481 5691 4481
Size: 1210 5379
BclI T'GATC_A
Cuts at: 1548 2356 3858 1548
Size: 808 1502 4279
Fragments arranged by size:
4279 1502 808
BfrBI ATG'CAT
Cuts at: 2464 2572 2464
Size: 108 6481
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5200 136
Size: 107 122 71 631 605 3528 1525
Fragments arranged by size:
3528 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3853 6584 3853
Size: 2731 3858
BlpI GC'TnA_GC
Cuts at: 4006 4006
Size: 6589
Bme1580I G_kGCm'C
Cuts at: 1790 3258 4650 5806 1790
Size: 1468 1392 1156 2573
Fragments arranged by size:
2573 1468 1392 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6589
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3139 4320 5278
Size: 416 53 83 399 24 1722 1181 958
Cuts at: 5278 442
Size: 1753
Fragments arranged by size:
1753 1722 1181 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6589
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2390 2390
Size: 6589
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2069 2126 2439 2946 3030 3044 3114 3282
Size: 830 57 313 507 84 14 70 168
Cuts at: 3282 3702 5249 1239
Size: 420 1547 2579
Fragments arranged by size:
2579 1547 830 507 420 313 168 84
70 57 14
Bpu10I CC'TnA_GC
Cuts at: 2375 2375
Size: 6589
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2465 2710 4714 5579 5877 2465
Size: 245 2004 865 298 3177
Fragments arranged by size:
3177 2004 865 298 245
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2102 2590 5252 882
Size: 617 603 488 2662 2219
Fragments arranged by size:
2662 2219 617 603 488
BsaAI yAC'GTr
Cuts at: 493 1204 2445 4380 493
Size: 711 1241 1935 2702
Fragments arranged by size:
2702 1935 1241 711
BsaBI GATnn'nnATC
Cuts at: 6583 6583
Size: 6589
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4396 4778 5413 275
Size: 53 83 186 936 2863 382 635 1451
Fragments arranged by size:
2863 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2756 5022 5420 5694 5841 6252 2756
Size: 2266 398 274 147 411 3093
Fragments arranged by size:
3093 2266 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3215 3245 1178
Size: 30 165 30 1812 30 4522
Fragments arranged by size:
4522 1812 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2066 3220 1186
Size: 880 1154 4555
Fragments arranged by size:
4555 1154 880
BseYI C'CCAG_C
Cuts at: 5792 6288 5792
Size: 496 6093
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3659 3841 1163
Size: 573 1923 182 3911
Fragments arranged by size:
3911 1923 573 182
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4039 4800 5828 1540
Size: 275 186 2038 761 1028 2301
Fragments arranged by size:
2301 2038 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3258 4650 4735 5806 3258
Size: 1392 85 1071 4041
Fragments arranged by size:
4041 1392 1071 85
BsiWI C'GTAC_G
Cuts at: 2445 2445
Size: 6589
BsmI GAATG_Cn'
Cuts at: 2574 3032 3116 3200 3215 3426 3452 3719 4100
Size: 458 84 84 15 211 26 267 381
Cuts at: 4100 4193 2574
Size: 93 4970
Fragments arranged by size:
4970 458 381 267 211 93 84 84
26 15
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3258 3908 4650 4735 5806 1391
Size: 399 1468 650 742 85 1071 2174
Fragments arranged by size:
2174 1468 1071 742 650 399 85
BspEI T'CCGG_A
Cuts at: 6252 6252
Size: 6589
BspHI T'CATG_A
Cuts at: 4478 6208 4478
Size: 1730 4859
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3944 844
Size: 3100 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4476 1394
Size: 429 2653 3507
Fragments arranged by size:
3507 2653 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2705 3293 5084 5258 65
Size: 1183 1457 588 1791 174 1396
Fragments arranged by size:
1791 1457 1396 1183 588 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5233 5420 1700
Size: 110 3423 187 2869
Fragments arranged by size:
3423 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2400 96
Size: 2304 4285
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6589
BssSI C'ACGA_G
Cuts at: 4649 5661 4649
Size: 1012 5577
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3204 794
Size: 2410 4179
BstBI TT'CG_AA
Cuts at: 2818 2902 2986 3070 3154 5393 2818
Size: 84 84 84 84 2239 4014
Fragments arranged by size:
4014 2239 84 84 84 84
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6589
BstXI CCAn_nnnn'nTGG
Cuts at: 2534 2534
Size: 6589
BstYI r'GATC_y
Cuts at: 1972 3853 3919 4286 4671 4688 6140 6226 6238
Size: 1881 66 367 385 17 1452 86 12
Cuts at: 6238 6584 1972
Size: 346 1977
Fragments arranged by size:
1977 1881 1452 385 367 346 86 66
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5098
Fragments arranged by size:
5098 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2450 487
Size: 683 56 1224 4626
Fragments arranged by size:
4626 1224 683 56
BtsI GCAGTG_nn'
Cuts at: 2670 3377 3545 3829 4105 4899 4919 2670
Size: 707 168 284 276 794 20 4340
Fragments arranged by size:
4340 794 707 284 276 168 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6589
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3019 3054 3103 3138 3271 3306 3439
Size: 35 2455 35 49 35 133 35 133
Cuts at: 3439 3474 3523 3558 529
Size: 35 49 35 3560
Fragments arranged by size:
3560 2455 133 133 49 49 35 35
35 35 35 35
DraI TTT'AAA
Cuts at: 3893 3893
Size: 6589
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2015 5596 809
Size: 507 699 3581 1802
Fragments arranged by size:
3581 1802 699 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3644 4036 4925
Size: 54 1425 50 275 18 1814 392 889
Cuts at: 4925 6554 8
Size: 1629 43
Fragments arranged by size:
1814 1629 1425 889 392 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4036 1537
Size: 275 2224 4090
Fragments arranged by size:
4090 2224 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4519 1353
Size: 3166 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5176 5550 5696 387
Size: 1064 138 3587 374 146 1280
Fragments arranged by size:
3587 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2069 2126 2439 2946 2950 3030 3044
Size: 393 437 57 313 507 4 80 14
Cuts at: 3044 3114 3282 3370 3450 3702 3813 4652 5249
Size: 70 168 88 80 252 111 839 597
Cuts at: 5249 6036 6304 1239
Size: 787 268 1524
Fragments arranged by size:
1524 839 787 597 507 437 393 313
268 252 168 111 88 80 80 70
57 14 4
EcoO109I rG'GnC_Cy
Cuts at: 2083 2083
Size: 6589
EcoRI G'AATT_C
Cuts at: 3812 3898 4276 3812
Size: 86 378 6125
Fragments arranged by size:
6125 378 86
FspI TGC'GCA
Cuts at: 5095 5095
Size: 6589
HaeII r_GCGC'y
Cuts at: 1678 5736 1678
Size: 4058 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2374 2406 3843 3875 5230
Size: 32 135 32 802 32 1437 32 1355
Cuts at: 5230 5262 5304 5336 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1437 1355 802 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3933 4179 669
Size: 988 281 1995 246 3079
Fragments arranged by size:
3079 1995 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2793 3465 3549 748
Size: 2045 672 84 3788
Fragments arranged by size:
3788 2045 672 84
HpaI GTT'AAC
Cuts at: 4179 4179
Size: 6589
KpnI G_GTAC'C
Cuts at: 3914 3914
Size: 6589
MfeI C'AATT_G
Cuts at: 3304 3724 4188 3304
Size: 420 464 5705
Fragments arranged by size:
5705 464 420
MluI A'CGCG_T
Cuts at: 6465 6465
Size: 6589
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2313 5703 5887 1305
Size: 1008 3390 184 2007
Fragments arranged by size:
3390 2007 1008 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3646 10
Size: 54 1425 343 1814 2953
Fragments arranged by size:
2953 1814 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2521 2773 2857 2941 3251 4547 4906
Size: 648 1355 252 84 84 310 1296 359
Cuts at: 4906 5065 518
Size: 159 2042
Fragments arranged by size:
2042 1355 1296 648 359 310 252 159
84 84
MspA1I CmG'CkG
Cuts at: 1606 4005 4682 5830 6075 1606
Size: 2399 677 1148 245 2120
Fragments arranged by size:
2399 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5098
Fragments arranged by size:
5098 939 552
NdeI CA'TA_TG
Cuts at: 387 2910 3330 3498 3666 387
Size: 2523 420 168 168 3310
Fragments arranged by size:
3310 2523 420 168 168
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6589
NotI GC'GGCC_GC
Cuts at: 4036 4036
Size: 6589
NsiI A_TGCA'T
Cuts at: 2466 2574 2466
Size: 108 6481
NspI r_CATG'y
Cuts at: 1132 1229 2576 2660 3416 3584 3752 3947 5492
Size: 97 1347 84 756 168 168 195 1545
Cuts at: 5492 1132
Size: 2229
Fragments arranged by size:
2229 1545 1347 756 195 168 168 97
84
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6589
PciI A'CATG_T
Cuts at: 3412 3580 3748 5488 3412
Size: 168 168 1740 4513
Fragments arranged by size:
4513 1740 168 168
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6589
PmeI GTTT'AAAC
Cuts at: 3893 3893
Size: 6589
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2111 2143 4658 4690 6171 6203 2111
Size: 32 2515 32 1481 32 2497
Fragments arranged by size:
2515 2497 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2083 2083
Size: 6589
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6589
PsiI TTA'TAA
Cuts at: 4159 4159
Size: 6589
PstI C_TGCA'G
Cuts at: 830 3941 830
Size: 3111 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6589
SalI G'TCGA_C
Cuts at: 1936 3931 1936
Size: 1995 4594
SbfI CC_TGCA'GG
Cuts at: 3941 3941
Size: 6589
ScaI AGT'ACT
Cuts at: 4837 6549 4837
Size: 1712 4877
SfcI C'TryA_G
Cuts at: 826 1045 2089 2545 3597 3765 3937 5072 5753
Size: 219 1044 456 1052 168 172 1135 681
Cuts at: 5753 5944 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1052 1044 681 456 219 191
172 168
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6589
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6589
SmaI CCC'GGG
Cuts at: 1872 3916 1872
Size: 2044 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2480 2725 3739 4693 5594 5856
Size: 197 926 537 245 1014 954 901 262
Cuts at: 5856 820
Size: 1553
Fragments arranged by size:
1553 1014 954 926 901 537 262 245
197
SnaBI TAC'GTA
Cuts at: 493 4380 493
Size: 3887 2702
SpeI A'CTAG_T
Cuts at: 152 2735 152
Size: 2583 4006
SphI G_CATG'C
Cuts at: 1132 3947 1132
Size: 2815 3774
SspI AAT'ATT
Cuts at: 5 52 4513 5
Size: 47 4461 2081
Fragments arranged by size:
4461 2081 47
StuI AGG'CCT
Cuts at: 1517 2543 2963 1517
Size: 1026 420 5143
Fragments arranged by size:
5143 1026 420
StyI C'CwwG_G
Cuts at: 513 1065 2004 2202 4028 513
Size: 552 939 198 1826 3074
Fragments arranged by size:
3074 1826 939 552 198
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4616 4633 4786 4971 5416 325
Size: 1255 3036 17 153 185 445 1498
Fragments arranged by size:
3036 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2400 4835 6547
Size: 274 80 33 51 1443 423 2435 1712
Cuts at: 6547 96
Size: 138
Fragments arranged by size:
2435 1712 1443 423 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3160 3192 3215 3247 6549 6581 3160
Size: 32 23 32 3302 32 3168
Fragments arranged by size:
3302 3168 32 32 32 23
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6589
XbaI T'CTAG_A
Cuts at: 3925 3925
Size: 6589
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 6589
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6589
XmaI C'CCGG_G
Cuts at: 1870 3914 1870
Size: 2044 4545
XmnI GAAnn'nnTTC
Cuts at: 2236 2795 3047 3084 3131 3215 3299 3383 3467
Size: 559 252 37 47 84 84 84 84
Cuts at: 3467 3551 3635 3719 4718 2236
Size: 84 84 84 999 4107
Fragments arranged by size:
4107 999 559 252 84 84 84 84
84 84 84 47 37
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4397 276
Size: 53 83 186 936 2863 2468
Fragments arranged by size:
2863 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI