Restriction map for
pFN21AB8342
(Circular) (Six-base) MAPSORT of: pFN21AB8342.seq Check: 895 from: 1 to: 5749
pFN21AB8342 5749 bp
With 182 enzymes: SgfI *
October 20, 2009 10:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3559 278
Size: 53 83 186 936 2023 2468
Fragments arranged by size:
2468 2023 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3092 1937
Size: 1155 4594
Acc65I G'GTAC_C
Cuts at: 3070 3070
Size: 5749
AclI AA'CG_TT
Cuts at: 1563 3876 4249 1563
Size: 2313 373 3063
Fragments arranged by size:
3063 2313 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3812 5196 5464 1632
Size: 2180 1384 268 1917
Fragments arranged by size:
2180 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5552
AflIII A'CryG_T
Cuts at: 1203 2133 2622 4648 5625 1203
Size: 930 489 2026 977 1327
Fragments arranged by size:
2026 1327 977 930 489
AgeI A'CCGG_T
Cuts at: 4580 4580
Size: 5749
AhdI GACnn_n'nnGTC
Cuts at: 4478 4478
Size: 5749
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5749
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5749
AlwNI CAG_nnn'CTG
Cuts at: 3492 5064 3492
Size: 1572 4177
ApaI G_GGCC'C
Cuts at: 2325 2325
Size: 5749
ApaLI G'TGCA_C
Cuts at: 3806 4962 3806
Size: 1156 4593
ApoI r'AATT_y
Cuts at: 1493 3058 3284 3436 5545 1493
Size: 1565 226 152 2109 1697
Fragments arranged by size:
2109 1697 1565 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4303 160
Size: 872 3271 1606
Fragments arranged by size:
3271 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5749
AvaI C'yCGr_G
Cuts at: 1870 1943 2543 3074 1870
Size: 73 600 531 4545
Fragments arranged by size:
4545 600 531 73
AvrII C'CTAG_G
Cuts at: 2266 2266
Size: 5749
BamHI G'GATC_C
Cuts at: 3079 3446 3079
Size: 367 5382
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2800 3070 4525 618
Size: 325 217 627 1013 270 1455 1842
Fragments arranged by size:
1842 1455 1013 627 325 270 217
BanII G_rGCy'C
Cuts at: 1391 2325 2846 3068 1391
Size: 934 521 222 4072
Fragments arranged by size:
4072 934 521 222
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2308 2677 928
Size: 971 409 369 4000
Fragments arranged by size:
4000 971 409 369
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2056 2090 3940 3974 1903
Size: 34 119 34 1850 34 3678
Fragments arranged by size:
3678 1850 119 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2300 3641 4851 2300
Size: 1341 1210 3198
Fragments arranged by size:
3198 1341 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5749
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2816 4360 136
Size: 107 122 71 631 605 1144 1544 1525
Fragments arranged by size:
1544 1525 1144 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5744 5744
Size: 5749
BlpI GC'TnA_GC
Cuts at: 2100 3166 2100
Size: 1066 4683
Bme1580I G_kGCm'C
Cuts at: 1790 2040 2325 2805 3810 4966 1790
Size: 250 285 480 1005 1156 2573
Fragments arranged by size:
2573 1156 1005 480 285 250
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5749
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2996 3480 4438
Size: 416 53 83 399 24 1579 484 958
Cuts at: 4438 442
Size: 1753
Fragments arranged by size:
1753 1579 958 484 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5749
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4409 1239
Size: 3170 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2570 3874 4739 5037 2570
Size: 1304 865 298 3282
Fragments arranged by size:
3282 1304 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4412 882
Size: 617 2913 2219
Fragments arranged by size:
2913 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2035 2413 3540 493
Size: 711 831 378 1127 2702
Fragments arranged by size:
2702 1127 831 711 378
BsaBI GATnn'nnATC
Cuts at: 2657 5743 2657
Size: 3086 2663
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3556 3938 4573 275
Size: 53 83 186 936 2023 382 635 1451
Fragments arranged by size:
2023 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2943 4182 4580 4854 5001 5412 2943
Size: 1239 398 274 147 411 3280
Fragments arranged by size:
3280 1239 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2828 2858 1178
Size: 30 165 30 1425 30 4069
Fragments arranged by size:
4069 1425 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5749
BseYI C'CCAG_C
Cuts at: 2152 2709 2796 2875 4952 5448 2152
Size: 557 87 79 2077 496 2453
Fragments arranged by size:
2453 2077 557 496 87 79
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5176
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3048 3199 3960 4988 1540
Size: 275 186 1047 151 761 1028 2301
Fragments arranged by size:
2301 1047 1028 761 275 186 151
BsiHKAI G_wGCw'C
Cuts at: 2561 3810 3895 4966 2561
Size: 1249 85 1071 3344
Fragments arranged by size:
3344 1249 1071 85
BsmI GAATG_Cn'
Cuts at: 2819 3260 3353 2819
Size: 441 93 5215
Fragments arranged by size:
5215 441 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3020 3020
Size: 5749
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2040 2325 2561 2805 2846 3068 3810
Size: 399 250 285 236 244 41 222 742
Cuts at: 3810 3895 4966 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 285 250 244 236
222 85 41
BspEI T'CCGG_A
Cuts at: 2943 5412 2943
Size: 2469 3280
BspHI T'CATG_A
Cuts at: 2865 2925 3638 5368 2865
Size: 60 713 1730 3246
Fragments arranged by size:
3246 1730 713 60
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3104 844
Size: 2260 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2407 3636 1394
Size: 429 584 1229 3507
Fragments arranged by size:
3507 1229 584 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4244 4418 65
Size: 1183 2996 174 1396
Fragments arranged by size:
2996 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2522 4393 4580 1700
Size: 110 712 1871 187 2869
Fragments arranged by size:
2869 1871 712 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5749
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5749
BssSI C'ACGA_G
Cuts at: 2560 3809 4821 2560
Size: 1249 1012 3488
Fragments arranged by size:
3488 1249 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5749
BstBI TT'CG_AA
Cuts at: 4553 4553
Size: 5749
BstEII G'GTnAC_C
Cuts at: 1184 2752 1184
Size: 1568 4181
BstYI r'GATC_y
Cuts at: 1972 3079 3446 3831 3848 5300 5386 5398 5744
Size: 1107 367 385 17 1452 86 12 346
Cuts at: 5744 1972
Size: 1977
Fragments arranged by size:
1977 1452 1107 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2105 2244 513
Size: 552 1040 139 4018
Fragments arranged by size:
4018 1040 552 139
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2764 487
Size: 683 56 1538 3472
Fragments arranged by size:
3472 1538 683 56
BtsI GCAGTG_nn'
Cuts at: 2561 3265 4059 4079 2561
Size: 704 794 20 4231
Fragments arranged by size:
4231 794 704 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5749
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5714
DraI TTT'AAA
Cuts at: 3053 3053
Size: 5749
DraIII CAC_nnn'GTG
Cuts at: 2990 2990
Size: 5749
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4756 809
Size: 507 3440 1802
Fragments arranged by size:
3440 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2124 2397 3045
Size: 54 1425 50 275 18 294 273 648
Cuts at: 3045 3196 4085 5714 8
Size: 151 889 1629 43
Fragments arranged by size:
1629 1425 889 648 294 275 273 151
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3045 3196 1537
Size: 275 1233 151 4090
Fragments arranged by size:
4090 1233 275 151
EarI CTCTTCn'nnn_
Cuts at: 1353 2261 3679 1353
Size: 908 1418 3423
Fragments arranged by size:
3423 1418 908
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4336 4710 4856 387
Size: 1064 138 2747 374 146 1280
Fragments arranged by size:
2747 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3812 4409 5196 5464 1239
Size: 393 2180 597 787 268 1524
Fragments arranged by size:
2180 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2021 2548 2021
Size: 527 5222
EcoRI G'AATT_C
Cuts at: 3058 3436 3058
Size: 378 5371
FspI TGC'GCA
Cuts at: 2210 4255 2210
Size: 2045 3704
HaeII r_GCGC'y
Cuts at: 1678 2498 2898 4896 1678
Size: 820 400 1998 2531
Fragments arranged by size:
2531 1998 820 400
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2298 2330 2987 3019 4390
Size: 32 135 32 726 32 657 32 1371
Cuts at: 4390 4422 4464 4496 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1371 726 657 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2599 3093 3339 669
Size: 988 281 661 494 246 3079
Fragments arranged by size:
3079 988 661 494 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5749
HpaI GTT'AAC
Cuts at: 3339 3339
Size: 5749
KpnI G_GTAC'C
Cuts at: 3074 3074
Size: 5749
MfeI C'AATT_G
Cuts at: 3348 3348
Size: 5749
MluI A'CGCG_T
Cuts at: 5625 5625
Size: 5749
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2871 4863 5047 1305
Size: 1566 1992 184 2007
Fragments arranged by size:
2007 1992 1566 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2399 10
Size: 54 1425 343 567 3360
Fragments arranged by size:
3360 1425 567 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2621 2627 2648 2759 2771 2864 3707
Size: 648 1455 6 21 111 12 93 843
Cuts at: 3707 4066 4225 518
Size: 359 159 2042
Fragments arranged by size:
2042 1455 843 648 359 159 111 93
21 12 6
MspA1I CmG'CkG
Cuts at: 1606 2705 3165 3842 4990 5235 1606
Size: 1099 460 677 1148 245 2120
Fragments arranged by size:
2120 1148 1099 677 460 245
NaeI GCC'GGC
Cuts at: 2524 2524
Size: 5749
NcoI C'CATG_G
Cuts at: 513 1065 2105 513
Size: 552 1040 4157
Fragments arranged by size:
4157 1040 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5749
NgoMIV G'CCGG_C
Cuts at: 2522 2522
Size: 5749
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5749
NotI GC'GGCC_GC
Cuts at: 3196 3196
Size: 5749
NspI r_CATG'y
Cuts at: 1132 1229 2137 2626 2956 3107 4652 1132
Size: 97 908 489 330 151 1545 2229
Fragments arranged by size:
2229 1545 908 489 330 151 97
PasI CC'CwG_GG
Cuts at: 1348 2359 1348
Size: 1011 4738
PciI A'CATG_T
Cuts at: 2133 2622 4648 2133
Size: 489 2026 3234
Fragments arranged by size:
3234 2026 489
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5749
PmeI GTTT'AAAC
Cuts at: 3053 3053
Size: 5749
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3818 3850 5331 5363 3818
Size: 32 1481 32 4204
Fragments arranged by size:
4204 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5749
PsiI TTA'TAA
Cuts at: 3319 3319
Size: 5749
PspOMI G'GGCC_C
Cuts at: 2321 2321
Size: 5749
PstI C_TGCA'G
Cuts at: 830 2556 3101 830
Size: 1726 545 3478
Fragments arranged by size:
3478 1726 545
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5749
SalI G'TCGA_C
Cuts at: 1936 3091 1936
Size: 1155 4594
SbfI CC_TGCA'GG
Cuts at: 3101 3101
Size: 5749
ScaI AGT'ACT
Cuts at: 2090 3997 5709 2090
Size: 1907 1712 2130
Fragments arranged by size:
2130 1907 1712
SfcI C'TryA_G
Cuts at: 826 1045 2552 3097 4232 4913 5104 826
Size: 219 1507 545 1135 681 191 1471
Fragments arranged by size:
1507 1471 1135 681 545 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5749
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5749
SmaI CCC'GGG
Cuts at: 1872 3076 1872
Size: 1204 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2585 3853 4754 5016 820
Size: 197 926 642 1268 901 262 1553
Fragments arranged by size:
1553 1268 926 901 642 262 197
SnaBI TAC'GTA
Cuts at: 493 3540 493
Size: 3047 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5749
SphI G_CATG'C
Cuts at: 1132 2956 3107 1132
Size: 1824 151 3774
Fragments arranged by size:
3774 1824 151
SspI AAT'ATT
Cuts at: 5 52 3673 5
Size: 47 3621 2081
Fragments arranged by size:
3621 2081 47
StuI AGG'CCT
Cuts at: 1517 3002 1517
Size: 1485 4264
StyI C'CwwG_G
Cuts at: 513 1065 2105 2266 2385 3188 513
Size: 552 1040 161 119 803 3074
Fragments arranged by size:
3074 1040 803 552 161 119
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2788 2843 2849 2978 3776 3793 3946
Size: 1255 1208 55 6 129 798 17 153
Cuts at: 3946 4131 4576 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1208 798 445 185 153 129
55 17 6
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2088 3995 5707
Size: 274 80 33 51 1443 111 1907 1712
Cuts at: 5707 96
Size: 138
Fragments arranged by size:
1907 1712 1443 274 138 111 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2828 2860 5709 5741 2828
Size: 32 2849 32 2836
Fragments arranged by size:
2849 2836 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5749
XbaI T'CTAG_A
Cuts at: 3085 3085
Size: 5749
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2523 2523
Size: 5749
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5749
XmaI C'CCGG_G
Cuts at: 1870 3074 1870
Size: 1204 4545
XmnI GAAnn'nnTTC
Cuts at: 3878 3878
Size: 5749
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3557 276
Size: 53 83 186 936 2023 2468
Fragments arranged by size:
2468 2023 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI