Restriction map for
pFN21AB8341
(Circular) (Six-base) MAPSORT of: pFN21AB8341.seq Check: 8084 from: 1 to: 6532
pFN21AB8341 6532 bp
With 182 enzymes: SgfI *
February 27, 2009 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4342 278
Size: 53 83 186 936 2806 2468
Fragments arranged by size:
2806 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3875 1937
Size: 1938 4594
Acc65I G'GTAC_C
Cuts at: 3853 3853
Size: 6532
AclI AA'CG_TT
Cuts at: 1563 4659 5032 1563
Size: 3096 373 3063
Fragments arranged by size:
3096 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2526 4595 5979 6247 1632
Size: 894 2069 1384 268 1917
Fragments arranged by size:
2069 1917 1384 894 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6335
AflIII A'CryG_T
Cuts at: 1203 2325 3764 5431 6408 1203
Size: 1122 1439 1667 977 1327
Fragments arranged by size:
1667 1439 1327 1122 977
AgeI A'CCGG_T
Cuts at: 5363 5363
Size: 6532
AhdI GACnn_n'nnGTC
Cuts at: 5261 5261
Size: 6532
AleI CACnn'nnGTG
Cuts at: 1166 2209 2574 3579 1166
Size: 1043 365 1005 4119
Fragments arranged by size:
4119 1043 1005 365
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6532
AlwNI CAG_nnn'CTG
Cuts at: 4275 5847 4275
Size: 1572 4960
ApaLI G'TGCA_C
Cuts at: 4589 5745 4589
Size: 1156 5376
ApoI r'AATT_y
Cuts at: 1493 2565 2649 2817 2901 3069 3237 3321 3405
Size: 1072 84 168 84 168 168 84 84
Cuts at: 3405 3486 3570 3841 4067 4219 6328 1493
Size: 81 84 271 226 152 2109 1697
Fragments arranged by size:
2109 1697 1072 271 226 168 168 168
152 84 84 84 84 84 81
AseI AT'TA_AT
Cuts at: 160 1032 5086 160
Size: 872 4054 1606
Fragments arranged by size:
4054 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6532
AvaI C'yCGr_G
Cuts at: 1870 1943 3857 1870
Size: 73 1914 4545
Fragments arranged by size:
4545 1914 73
BamHI G'GATC_C
Cuts at: 3738 3862 4229 3738
Size: 124 367 6041
Fragments arranged by size:
6041 367 124
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3853 5308 618
Size: 325 217 627 2066 1455 1842
Fragments arranged by size:
2066 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3851 1391
Size: 2460 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3119 3452 928
Size: 971 1220 333 4008
Fragments arranged by size:
4008 1220 971 333
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4723 4757 1903
Size: 34 50 34 2702 34 3678
Fragments arranged by size:
3678 2702 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4424 5634 4424
Size: 1210 5322
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6532
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5143 136
Size: 107 122 71 631 605 3471 1525
Fragments arranged by size:
3471 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6527 6527
Size: 6532
BlpI GC'TnA_GC
Cuts at: 3949 3949
Size: 6532
Bme1580I G_kGCm'C
Cuts at: 1790 4593 5749 1790
Size: 2803 1156 2573
Fragments arranged by size:
2803 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6532
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4263 5221 442
Size: 416 53 83 399 24 2846 958 1753
Fragments arranged by size:
2846 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6532
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2160 2192 3684 5192 1239
Size: 921 32 1492 1508 2579
Fragments arranged by size:
2579 1508 1492 921 32
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4657 5522 5820 4657
Size: 865 298 5369
Fragments arranged by size:
5369 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3163 5195 882
Size: 617 1664 2032 2219
Fragments arranged by size:
2219 2032 1664 617
BsaAI yAC'GTr
Cuts at: 493 1204 3458 4323 493
Size: 711 2254 865 2702
Fragments arranged by size:
2702 2254 865 711
BsaBI GATnn'nnATC
Cuts at: 6526 6526
Size: 6532
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4339 4721 5356 275
Size: 53 83 186 936 2806 382 635 1451
Fragments arranged by size:
2806 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3245 4965 5363 5637 5784 6195 3245
Size: 1720 398 274 147 411 3582
Fragments arranged by size:
3582 1720 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6307
Fragments arranged by size:
6307 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6532
BseYI C'CCAG_C
Cuts at: 5735 6231 5735
Size: 496 6036
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5959
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3982 4743 5771 1540
Size: 275 186 1981 761 1028 2301
Fragments arranged by size:
2301 1981 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4593 4678 5749 4593
Size: 85 1071 5376
Fragments arranged by size:
5376 1071 85
BsmI GAATG_Cn'
Cuts at: 2072 2438 3110 4043 4136 2072
Size: 366 672 933 93 4468
Fragments arranged by size:
4468 933 672 366 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3851 4593 4678 5749 1391
Size: 399 2061 742 85 1071 2174
Fragments arranged by size:
2174 2061 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6195 6195
Size: 6532
BspHI T'CATG_A
Cuts at: 4421 6151 4421
Size: 1730 4802
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3887 844
Size: 3043 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4419 1394
Size: 429 2596 3507
Fragments arranged by size:
3507 2596 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5027 5201 65
Size: 1183 3779 174 1396
Fragments arranged by size:
3779 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5176 5363 1700
Size: 110 3366 187 2869
Fragments arranged by size:
3366 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2094 2418 96
Size: 1998 324 4210
Fragments arranged by size:
4210 1998 324
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6532
BssSI C'ACGA_G
Cuts at: 4592 5604 4592
Size: 1012 5520
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6532
BstBI TT'CG_AA
Cuts at: 5336 5336
Size: 6532
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6532
BstYI r'GATC_y
Cuts at: 1972 3738 3862 4229 4614 4631 6083 6169 6181
Size: 1766 124 367 385 17 1452 86 12
Cuts at: 6181 6527 1972
Size: 346 1977
Fragments arranged by size:
1977 1766 1452 385 367 346 124 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5980
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5793
Fragments arranged by size:
5793 683 56
BtsI GCAGTG_nn'
Cuts at: 4048 4842 4862 4048
Size: 794 20 5718
Fragments arranged by size:
5718 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6532
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3172 3207 529
Size: 35 2608 35 3854
Fragments arranged by size:
3854 2608 35 35
DraI TTT'AAA
Cuts at: 3836 3836
Size: 6532
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5539 809
Size: 507 4223 1802
Fragments arranged by size:
4223 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2037 3979 4868
Size: 54 1425 50 275 18 207 1942 889
Cuts at: 4868 6497 8
Size: 1629 43
Fragments arranged by size:
1942 1629 1425 889 275 207 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3979 1537
Size: 275 2167 4090
Fragments arranged by size:
4090 2167 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2383 2811 4462 1353
Size: 1030 428 1651 3423
Fragments arranged by size:
3423 1651 1030 428
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5119 5493 5639 387
Size: 1064 138 3530 374 146 1280
Fragments arranged by size:
3530 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2160 2192 2526 3684 4595 5192 5979
Size: 393 528 32 334 1158 911 597 787
Cuts at: 5979 6247 1239
Size: 268 1524
Fragments arranged by size:
1524 1158 911 787 597 528 393 334
268 32
EcoO109I rG'GnC_Cy
Cuts at: 3699 3699
Size: 6532
EcoRI G'AATT_C
Cuts at: 2565 2817 2901 3069 3237 3321 3486 3570 3841
Size: 252 84 168 168 84 165 84 271
Cuts at: 3841 4219 2565
Size: 378 4878
Fragments arranged by size:
4878 378 271 252 168 168 165 84
84 84
FspI TGC'GCA
Cuts at: 5038 5038
Size: 6532
HaeII r_GCGC'y
Cuts at: 1678 5679 1678
Size: 4001 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2132 2164 5173 5205 5247
Size: 32 135 32 560 32 3009 32 42
Cuts at: 5247 5279 1373
Size: 32 2626
Fragments arranged by size:
3009 2626 560 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3876 4122 669
Size: 988 281 1938 246 3079
Fragments arranged by size:
3079 1938 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2694 748
Size: 1946 4586
HpaI GTT'AAC
Cuts at: 4122 4122
Size: 6532
KpnI G_GTAC'C
Cuts at: 3857 3857
Size: 6532
MfeI C'AATT_G
Cuts at: 4131 4131
Size: 6532
MluI A'CGCG_T
Cuts at: 6408 6408
Size: 6532
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2091 5646 5830 1305
Size: 786 3555 184 2007
Fragments arranged by size:
3555 2007 786 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2039 10
Size: 54 1425 343 207 4503
Fragments arranged by size:
4503 1425 343 207 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2209 2574 2658 2758 2826 2910 2994
Size: 648 1043 365 84 100 68 84 84
Cuts at: 2994 3162 3330 3495 3579 3747 4490 4849 5008
Size: 168 168 165 84 168 743 359 159
Cuts at: 5008 518
Size: 2042
Fragments arranged by size:
2042 1043 743 648 365 359 168 168
168 165 159 100 84 84 84 84
68
MspA1I CmG'CkG
Cuts at: 1606 3948 4625 5773 6018 1606
Size: 2342 677 1148 245 2120
Fragments arranged by size:
2342 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5980
NdeI CA'TA_TG
Cuts at: 387 2755 3011 387
Size: 2368 256 3908
Fragments arranged by size:
3908 2368 256
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6532
NotI GC'GGCC_GC
Cuts at: 3979 3979
Size: 6532
NspI r_CATG'y
Cuts at: 1132 1229 2329 2688 3768 3890 5435 1132
Size: 97 1100 359 1080 122 1545 2229
Fragments arranged by size:
2229 1545 1100 1080 359 122 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6532
PciI A'CATG_T
Cuts at: 2325 3764 5431 2325
Size: 1439 1667 3426
Fragments arranged by size:
3426 1667 1439
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2017 1108
Size: 909 5623
PmeI GTTT'AAAC
Cuts at: 3836 3836
Size: 6532
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4601 4633 6114 6146 4601
Size: 32 1481 32 4987
Fragments arranged by size:
4987 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6532
PsiI TTA'TAA
Cuts at: 3177 4102 3177
Size: 925 5607
PstI C_TGCA'G
Cuts at: 830 3884 830
Size: 3054 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6532
SalI G'TCGA_C
Cuts at: 1936 3874 1936
Size: 1938 4594
SbfI CC_TGCA'GG
Cuts at: 3884 3884
Size: 6532
ScaI AGT'ACT
Cuts at: 4780 6492 4780
Size: 1712 4820
SfcI C'TryA_G
Cuts at: 826 1045 2119 3880 5015 5696 5887 826
Size: 219 1074 1761 1135 681 191 1471
Fragments arranged by size:
1761 1471 1135 1074 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6532
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6532
SmaI CCC'GGG
Cuts at: 1872 3859 1872
Size: 1987 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4636 5537 5799 820
Size: 197 926 2693 901 262 1553
Fragments arranged by size:
2693 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4323 493
Size: 3830 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6532
SphI G_CATG'C
Cuts at: 1132 3890 1132
Size: 2758 3774
SspI AAT'ATT
Cuts at: 5 52 4456 5
Size: 47 4404 2081
Fragments arranged by size:
4404 2081 47
StuI AGG'CCT
Cuts at: 1517 2612 2864 2948 3284 3368 3533 1517
Size: 1095 252 84 336 84 165 4516
Fragments arranged by size:
4516 1095 336 252 165 84 84
StyI C'CwwG_G
Cuts at: 513 1065 2406 3971 513
Size: 552 1341 1565 3074
Fragments arranged by size:
3074 1565 1341 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4559 4576 4729 4914 5359 325
Size: 1255 2979 17 153 185 445 1498
Fragments arranged by size:
2979 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2094 2418 4778
Size: 274 80 33 51 1443 117 324 2360
Cuts at: 4778 6490 96
Size: 1712 138
Fragments arranged by size:
2360 1712 1443 324 274 138 117 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6492 6524 6492
Size: 32 6500
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6532
XbaI T'CTAG_A
Cuts at: 3868 3868
Size: 6532
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6532
XmaI C'CCGG_G
Cuts at: 1870 3857 1870
Size: 1987 4545
XmnI GAAnn'nnTTC
Cuts at: 4661 4661
Size: 6532
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4340 276
Size: 53 83 186 936 2806 2468
Fragments arranged by size:
2806 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI