Restriction map for
pFN21AB8340
(Circular) (Six-base) MAPSORT of: pFN21AB8340.seq Check: 1925 from: 1 to: 4924
pFN21AB8340 4924 bp
With 182 enzymes: SgfI *
February 27, 2009 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2734 278
Size: 53 83 186 936 1198 2468
Fragments arranged by size:
2468 1198 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2076 2267 1937
Size: 139 191 4594
Fragments arranged by size:
4594 191 139
Acc65I G'GTAC_C
Cuts at: 2245 2245
Size: 4924
AclI AA'CG_TT
Cuts at: 1563 3051 3424 1563
Size: 1488 373 3063
Fragments arranged by size:
3063 1488 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2987 4371 4639 1632
Size: 1355 1384 268 1917
Fragments arranged by size:
1917 1384 1355 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4727
AflIII A'CryG_T
Cuts at: 1203 3823 4800 1203
Size: 2620 977 1327
Fragments arranged by size:
2620 1327 977
AgeI A'CCGG_T
Cuts at: 3755 3755
Size: 4924
AhdI GACnn_n'nnGTC
Cuts at: 3653 3653
Size: 4924
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 4924
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 4924
AlwNI CAG_nnn'CTG
Cuts at: 2167 2667 4239 2167
Size: 500 1572 2852
Fragments arranged by size:
2852 1572 500
ApaLI G'TGCA_C
Cuts at: 2981 4137 2981
Size: 1156 3768
ApoI r'AATT_y
Cuts at: 1493 2233 2459 2611 4720 1493
Size: 740 226 152 2109 1697
Fragments arranged by size:
2109 1697 740 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3478 160
Size: 872 2446 1606
Fragments arranged by size:
2446 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4924
AvaI C'yCGr_G
Cuts at: 1870 1943 2249 1870
Size: 73 306 4545
Fragments arranged by size:
4545 306 73
BamHI G'GATC_C
Cuts at: 2254 2621 2254
Size: 367 4557
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2245 3700 618
Size: 325 217 627 458 1455 1842
Fragments arranged by size:
1842 1455 627 458 325 217
BanII G_rGCy'C
Cuts at: 1391 2243 1391
Size: 852 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 3953
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3115 3149 1903
Size: 34 1178 34 3678
Fragments arranged by size:
3678 1178 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2816 4026 2816
Size: 1210 3714
BclI T'GATC_A
Cuts at: 1548 1548
Size: 4924
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3535 136
Size: 107 122 71 631 605 337 1526 1525
Fragments arranged by size:
1526 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 4919 4919
Size: 4924
BlpI GC'TnA_GC
Cuts at: 2341 2341
Size: 4924
Bme1580I G_kGCm'C
Cuts at: 1790 2985 4141 1790
Size: 1195 1156 2573
Fragments arranged by size:
2573 1195 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 4924
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2655 3613 442
Size: 416 53 83 399 24 1238 958 1753
Fragments arranged by size:
1753 1238 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 4924
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2075 3584 1239
Size: 836 1509 2579
Fragments arranged by size:
2579 1509 836
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3049 3914 4212 3049
Size: 865 298 3761
Fragments arranged by size:
3761 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2009 2135 3587 882
Size: 617 510 126 1452 2219
Fragments arranged by size:
2219 1452 617 510 126
BsaAI yAC'GTr
Cuts at: 493 1204 2715 493
Size: 711 1511 2702
Fragments arranged by size:
2702 1511 711
BsaBI GATnn'nnATC
Cuts at: 4918 4918
Size: 4924
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2731 3113 3748 275
Size: 53 83 186 936 1198 382 635 1451
Fragments arranged by size:
1451 1198 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3357 3755 4029 4176 4587 3357
Size: 398 274 147 411 3694
Fragments arranged by size:
3694 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4699
Fragments arranged by size:
4699 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2150 2153 1186
Size: 964 3 3957
Fragments arranged by size:
3957 964 3
BseYI C'CCAG_C
Cuts at: 4127 4623 4127
Size: 496 4428
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2227 1163
Size: 573 491 3860
Fragments arranged by size:
3860 573 491
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2118 2374 3135 4163 1540
Size: 275 186 117 256 761 1028 2301
Fragments arranged by size:
2301 1028 761 275 256 186 117
BsiHKAI G_wGCw'C
Cuts at: 2985 3070 4141 2985
Size: 85 1071 3768
Fragments arranged by size:
3768 1071 85
BsmI GAATG_Cn'
Cuts at: 2435 2528 2435
Size: 93 4831
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2243 2985 3070 4141 1391
Size: 399 453 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 453 399 85
BspEI T'CCGG_A
Cuts at: 4587 4587
Size: 4924
BspHI T'CATG_A
Cuts at: 2813 4543 2813
Size: 1730 3194
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2279 844
Size: 1435 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2025 2811 1394
Size: 429 202 786 3507
Fragments arranged by size:
3507 786 429 202
BsrDI GCAATG_nn'
Cuts at: 65 1248 3419 3593 65
Size: 1183 2171 174 1396
Fragments arranged by size:
2171 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3568 3755 1700
Size: 110 1758 187 2869
Fragments arranged by size:
2869 1758 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 4924
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 4924
BssSI C'ACGA_G
Cuts at: 2984 3996 2984
Size: 1012 3912
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 4924
BstBI TT'CG_AA
Cuts at: 3728 3728
Size: 4924
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 4924
BstYI r'GATC_y
Cuts at: 1972 2210 2254 2621 3006 3023 4475 4561 4573
Size: 238 44 367 385 17 1452 86 12
Cuts at: 4573 4919 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 346 238 86 44
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4372
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2095 487
Size: 683 56 869 3316
Fragments arranged by size:
3316 869 683 56
BtsI GCAGTG_nn'
Cuts at: 2440 3234 3254 2440
Size: 794 20 4110
Fragments arranged by size:
4110 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 4924
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 4889
DraI TTT'AAA
Cuts at: 2228 2228
Size: 4924
DraIII CAC_nnn'GTG
Cuts at: 2103 2103
Size: 4924
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3931 809
Size: 507 2615 1802
Fragments arranged by size:
2615 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2115 2371 3260
Size: 54 1425 50 275 18 285 256 889
Cuts at: 3260 4889 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 285 275 256 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2115 2371 1537
Size: 275 303 256 4090
Fragments arranged by size:
4090 303 275 256
EarI CTCTTCn'nnn_
Cuts at: 1353 2854 1353
Size: 1501 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2027 3511 3885 4031 387
Size: 1064 138 438 1484 374 146 1280
Fragments arranged by size:
1484 1280 1064 438 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2075 2987 3584 4371 4639 1239
Size: 393 443 912 597 787 268 1524
Fragments arranged by size:
1524 912 787 597 443 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2132 2132
Size: 4924
EcoRI G'AATT_C
Cuts at: 2233 2611 2233
Size: 378 4546
FspI TGC'GCA
Cuts at: 3430 3430
Size: 4924
HaeII r_GCGC'y
Cuts at: 1678 2167 2185 4071 1678
Size: 489 18 1886 2531
Fragments arranged by size:
2531 1886 489 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2195 2227 3565 3597 3639
Size: 32 135 32 623 32 1338 32 42
Cuts at: 3639 3671 1373
Size: 32 2626
Fragments arranged by size:
2626 1338 623 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2077 2268 2514 669
Size: 988 281 139 191 246 3079
Fragments arranged by size:
3079 988 281 246 191 139
HindIII A'AGCT_T
Cuts at: 748 748
Size: 4924
HpaI GTT'AAC
Cuts at: 2514 2514
Size: 4924
KpnI G_GTAC'C
Cuts at: 2249 2249
Size: 4924
MfeI C'AATT_G
Cuts at: 2523 2523
Size: 4924
MluI A'CGCG_T
Cuts at: 4800 4800
Size: 4924
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4038 4222 1305
Size: 2733 184 2007
Fragments arranged by size:
2733 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3102
Fragments arranged by size:
3102 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2882 3241 3400 518
Size: 648 1716 359 159 2042
Fragments arranged by size:
2042 1716 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2340 3017 4165 4410 1606
Size: 734 677 1148 245 2120
Fragments arranged by size:
2120 1148 734 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4372
NdeI CA'TA_TG
Cuts at: 387 387
Size: 4924
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 4924
NotI GC'GGCC_GC
Cuts at: 2371 2371
Size: 4924
NspI r_CATG'y
Cuts at: 1132 1229 2282 3827 1132
Size: 97 1053 1545 2229
Fragments arranged by size:
2229 1545 1053 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 4924
PciI A'CATG_T
Cuts at: 3823 3823
Size: 4924
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 4924
PfoI T'CCnGG_A
Cuts at: 2052 2052
Size: 4924
PmeI GTTT'AAAC
Cuts at: 2228 2228
Size: 4924
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2993 3025 4506 4538 2993
Size: 32 1481 32 3379
Fragments arranged by size:
3379 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 4924
PsiI TTA'TAA
Cuts at: 2494 2494
Size: 4924
PstI C_TGCA'G
Cuts at: 830 2276 830
Size: 1446 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 4924
SalI G'TCGA_C
Cuts at: 1936 2075 2266 1936
Size: 139 191 4594
Fragments arranged by size:
4594 191 139
SbfI CC_TGCA'GG
Cuts at: 2276 2276
Size: 4924
ScaI AGT'ACT
Cuts at: 3172 4884 3172
Size: 1712 3212
SexAI A'CCwGG_T
Cuts at: 2040 2040
Size: 4924
SfcI C'TryA_G
Cuts at: 826 1045 2272 3407 4088 4279 826
Size: 219 1227 1135 681 191 1471
Fragments arranged by size:
1471 1227 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4924
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 4924
SmaI CCC'GGG
Cuts at: 1872 2251 1872
Size: 379 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3028 3929 4191 820
Size: 197 926 1085 901 262 1553
Fragments arranged by size:
1553 1085 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2715 493
Size: 2222 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 4924
SphI G_CATG'C
Cuts at: 1132 2282 1132
Size: 1150 3774
SspI AAT'ATT
Cuts at: 5 52 2848 5
Size: 47 2796 2081
Fragments arranged by size:
2796 2081 47
StuI AGG'CCT
Cuts at: 1517 2127 2193 1517
Size: 610 66 4248
Fragments arranged by size:
4248 610 66
StyI C'CwwG_G
Cuts at: 513 1065 2363 513
Size: 552 1298 3074
Fragments arranged by size:
3074 1298 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2951 2968 3121 3306 3751 325
Size: 1255 1371 17 153 185 445 1498
Fragments arranged by size:
1498 1371 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2058 3170 4882
Size: 274 80 33 51 1443 81 1112 1712
Cuts at: 4882 96
Size: 138
Fragments arranged by size:
1712 1443 1112 274 138 81 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2075 2107 4884 4916 2075
Size: 32 2777 32 2083
Fragments arranged by size:
2777 2083 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 4924
XbaI T'CTAG_A
Cuts at: 2260 2260
Size: 4924
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 4924
XmaI C'CCGG_G
Cuts at: 1870 2249 1870
Size: 379 4545
XmnI GAAnn'nnTTC
Cuts at: 3053 3053
Size: 4924
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2732 276
Size: 53 83 186 936 1198 2468
Fragments arranged by size:
2468 1198 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SfiI SfoI SrfI SwaI XcmI