Restriction map for
pFN21AB8339
(Circular) (Six-base) MAPSORT of: pFN21AB8339.seq Check: 5475 from: 1 to: 5635
pFN21AB8339 5635 bp
With 182 enzymes: SgfI *
February 27, 2009 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3445 278
Size: 53 83 186 936 1909 2468
Fragments arranged by size:
2468 1909 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2978 1937
Size: 1041 4594
Acc65I G'GTAC_C
Cuts at: 2393 2956 2393
Size: 563 5072
AclI AA'CG_TT
Cuts at: 1563 2934 3762 4135 1563
Size: 1371 828 373 3063
Fragments arranged by size:
3063 1371 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2811 3698 5082 5350 1632
Size: 1179 887 1384 268 1917
Fragments arranged by size:
1917 1384 1179 887 268
AfeI AGC'GCT
Cuts at: 2531 2531
Size: 5635
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5438
AflIII A'CryG_T
Cuts at: 1203 4534 5511 1203
Size: 3331 977 1327
Fragments arranged by size:
3331 1327 977
AgeI A'CCGG_T
Cuts at: 4466 4466
Size: 5635
AhdI GACnn_n'nnGTC
Cuts at: 4364 4364
Size: 5635
AleI CACnn'nnGTG
Cuts at: 1166 2372 1166
Size: 1206 4429
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5635
AlwNI CAG_nnn'CTG
Cuts at: 2339 2417 3378 4950 2339
Size: 78 961 1572 3024
Fragments arranged by size:
3024 1572 961 78
ApaLI G'TGCA_C
Cuts at: 3692 4848 3692
Size: 1156 4479
ApoI r'AATT_y
Cuts at: 1493 2944 3170 3322 5431 1493
Size: 1451 226 152 2109 1697
Fragments arranged by size:
2109 1697 1451 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4189 160
Size: 872 3157 1606
Fragments arranged by size:
3157 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5635
AvaI C'yCGr_G
Cuts at: 1870 1943 2589 2960 1870
Size: 73 646 371 4545
Fragments arranged by size:
4545 646 371 73
AvrII C'CTAG_G
Cuts at: 2543 2543
Size: 5635
BamHI G'GATC_C
Cuts at: 2965 3332 2965
Size: 367 5268
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2240 2393 2895 2956 4411
Size: 325 217 627 453 153 502 61 1455
Cuts at: 4411 618
Size: 1842
Fragments arranged by size:
1842 1455 627 502 453 325 217 153
61
BanII G_rGCy'C
Cuts at: 1391 2438 2611 2887 2954 1391
Size: 1047 173 276 67 4072
Fragments arranged by size:
4072 1047 276 173 67
BbeI G_GCGC'C
Cuts at: 2244 2244
Size: 5635
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2088 2146 928
Size: 971 189 58 4417
Fragments arranged by size:
4417 971 189 58
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3826 3860 1903
Size: 34 1889 34 3678
Fragments arranged by size:
3678 1889 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3527 4737 3527
Size: 1210 4425
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5635
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4246 136
Size: 107 122 71 631 605 2574 1525
Fragments arranged by size:
2574 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2854 5630 2854
Size: 2776 2859
BlpI GC'TnA_GC
Cuts at: 2610 3052 2610
Size: 442 5193
Bme1580I G_kGCm'C
Cuts at: 1790 3696 4852 1790
Size: 1906 1156 2573
Fragments arranged by size:
2573 1906 1156
BmgBI CAC'GTC
Cuts at: 1318 2833 1318
Size: 1515 4120
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2085 3366 4324
Size: 416 53 83 399 24 668 1281 958
Cuts at: 4324 442
Size: 1753
Fragments arranged by size:
1753 1281 958 668 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5635
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4295 1239
Size: 3056 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3760 4625 4923 3760
Size: 865 298 4472
Fragments arranged by size:
4472 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4298 882
Size: 617 2799 2219
Fragments arranged by size:
2799 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3426 493
Size: 711 2222 2702
Fragments arranged by size:
2702 2222 711
BsaBI GATnn'nnATC
Cuts at: 5629 5629
Size: 5635
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2241 3442 3824 4459
Size: 53 83 186 936 708 1201 382 635
Cuts at: 4459 275
Size: 1451
Fragments arranged by size:
1451 1201 936 708 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4068 4466 4740 4887 5298 4068
Size: 398 274 147 411 4405
Fragments arranged by size:
4405 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5410
Fragments arranged by size:
5410 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5635
BseYI C'CCAG_C
Cuts at: 2363 2449 4838 5334 2363
Size: 86 2389 496 2664
Fragments arranged by size:
2664 2389 496 86
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2069 2085 2684 1163
Size: 573 333 16 599 4114
Fragments arranged by size:
4114 599 573 333 16
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3085 3846 4874 1540
Size: 275 186 1084 761 1028 2301
Fragments arranged by size:
2301 1084 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2705 2887 3696 3781 4852 2705
Size: 182 809 85 1071 3488
Fragments arranged by size:
3488 1071 809 182 85
BsmI GAATG_Cn'
Cuts at: 3146 3239 3146
Size: 93 5542
BsmBI CGTCTCn'nnnn_
Cuts at: 2336 2336
Size: 5635
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2438 2611 2705 2887 2954 3696 3781
Size: 399 648 173 94 182 67 742 85
Cuts at: 3781 4852 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 648 399 182 173 94
85 67
BspEI T'CCGG_A
Cuts at: 5298 5298
Size: 5635
BspHI T'CATG_A
Cuts at: 3524 5254 3524
Size: 1730 3905
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2990 844
Size: 2146 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3522 1394
Size: 429 1699 3507
Fragments arranged by size:
3507 1699 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4130 4304 65
Size: 1183 2882 174 1396
Fragments arranged by size:
2882 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4279 4466 1700
Size: 110 2469 187 2869
Fragments arranged by size:
2869 2469 187 110
BsrGI T'GTAC_A
Cuts at: 96 2184 96
Size: 2088 3547
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5635
BssSI C'ACGA_G
Cuts at: 3695 4707 3695
Size: 1012 4623
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5635
BstBI TT'CG_AA
Cuts at: 4439 4439
Size: 5635
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5635
BstYI r'GATC_y
Cuts at: 1972 2854 2965 3332 3717 3734 5186 5272 5284
Size: 882 111 367 385 17 1452 86 12
Cuts at: 5284 5630 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 882 385 367 346 111 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2899 513
Size: 552 1834 3249
Fragments arranged by size:
3249 1834 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4896
Fragments arranged by size:
4896 683 56
BtsI GCAGTG_nn'
Cuts at: 2073 2379 2444 3151 3945 3965 2073
Size: 306 65 707 794 20 3743
Fragments arranged by size:
3743 794 707 306 65 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5635
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2076 2111 529
Size: 35 1512 35 4053
Fragments arranged by size:
4053 1512 35 35
DraI TTT'AAA
Cuts at: 2939 2939
Size: 5635
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2141 4642 809
Size: 507 825 2501 1802
Fragments arranged by size:
2501 1802 825 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2221 2713 3082
Size: 54 1425 50 275 18 391 492 369
Cuts at: 3082 3971 5600 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 492 391 369 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3082 1537
Size: 275 1270 4090
Fragments arranged by size:
4090 1270 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2031 3565 1353
Size: 678 1534 3423
Fragments arranged by size:
3423 1534 678
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4222 4596 4742 387
Size: 1064 138 2633 374 146 1280
Fragments arranged by size:
2633 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2885 2885
Size: 5635
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2811 3698 4295 5082 5350 1239
Size: 393 1179 887 597 787 268 1524
Fragments arranged by size:
1524 1179 887 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2944 3322 2944
Size: 378 5257
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2170 2170
Size: 5635
FspI TGC'GCA
Cuts at: 4141 4141
Size: 5635
HaeII r_GCGC'y
Cuts at: 1678 2244 2533 4782 1678
Size: 566 289 2249 2531
Fragments arranged by size:
2531 2249 566 289
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2631 2663 2895 2927 4276
Size: 32 135 32 1059 32 232 32 1349
Cuts at: 4276 4308 4350 4382 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1349 1059 232 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2320 2979 3225 669
Size: 988 281 382 659 246 3079
Fragments arranged by size:
3079 988 659 382 281 246
HindIII A'AGCT_T
Cuts at: 748 2742 748
Size: 1994 3641
HpaI GTT'AAC
Cuts at: 3225 3225
Size: 5635
KasI G'GCGC_C
Cuts at: 2240 2240
Size: 5635
KpnI G_GTAC'C
Cuts at: 2397 2960 2397
Size: 563 5072
MfeI C'AATT_G
Cuts at: 2535 3234 2535
Size: 699 4936
MluI A'CGCG_T
Cuts at: 5511 5511
Size: 5635
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4749 4933 1305
Size: 3444 184 2007
Fragments arranged by size:
3444 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2223 2715 10
Size: 54 1425 343 391 492 2930
Fragments arranged by size:
2930 1425 492 391 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2372 3593 3952 4111 518
Size: 648 1206 1221 359 159 2042
Fragments arranged by size:
2042 1221 1206 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2027 2120 2215 2336 2414 2821 3051 3728
Size: 421 93 95 121 78 407 230 677
Cuts at: 3728 4876 5121 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 421 407 245 230 121
95 93 78
NarI GG'CG_CC
Cuts at: 2241 2241
Size: 5635
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5083
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5635
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5635
NotI GC'GGCC_GC
Cuts at: 3082 3082
Size: 5635
NspI r_CATG'y
Cuts at: 1132 1229 2993 4538 1132
Size: 97 1764 1545 2229
Fragments arranged by size:
2229 1764 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2431 1348
Size: 1083 4552
PciI A'CATG_T
Cuts at: 4534 4534
Size: 5635
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5635
PfoI T'CCnGG_A
Cuts at: 2757 2757
Size: 5635
PmeI GTTT'AAAC
Cuts at: 2939 2939
Size: 5635
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3704 3736 5217 5249 3704
Size: 32 1481 32 4090
Fragments arranged by size:
4090 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5635
PsiI TTA'TAA
Cuts at: 3205 3205
Size: 5635
PstI C_TGCA'G
Cuts at: 830 2517 2802 2820 2987 830
Size: 1687 285 18 167 3478
Fragments arranged by size:
3478 1687 285 167 18
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5635
PvuII CAG'CTG
Cuts at: 2027 2120 2215 2336 2414 2821 2027
Size: 93 95 121 78 407 4841
Fragments arranged by size:
4841 407 121 95 93 78
SacI G_AGCT'C
Cuts at: 2887 2887
Size: 5635
SalI G'TCGA_C
Cuts at: 1936 2977 1936
Size: 1041 4594
SbfI CC_TGCA'GG
Cuts at: 2987 2987
Size: 5635
ScaI AGT'ACT
Cuts at: 2511 3883 5595 2511
Size: 1372 1712 2551
Fragments arranged by size:
2551 1712 1372
SfcI C'TryA_G
Cuts at: 826 1045 2513 2798 2816 2983 4118 4799 4990
Size: 219 1468 285 18 167 1135 681 191
Cuts at: 4990 826
Size: 1471
Fragments arranged by size:
1471 1468 1135 681 285 219 191 167
18
SfoI GGC'GCC
Cuts at: 2242 2242
Size: 5635
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5635
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5635
SmaI CCC'GGG
Cuts at: 1872 2962 1872
Size: 1090 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3739 4640 4902 820
Size: 197 926 1796 901 262 1553
Fragments arranged by size:
1796 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3426 493
Size: 2933 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5635
SphI G_CATG'C
Cuts at: 1132 2993 1132
Size: 1861 3774
SspI AAT'ATT
Cuts at: 5 52 3559 5
Size: 47 3507 2081
Fragments arranged by size:
3507 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5635
StyI C'CwwG_G
Cuts at: 513 1065 2543 3074 513
Size: 552 1478 531 3074
Fragments arranged by size:
3074 1478 552 531
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3662 3679 3832 4017 4462 325
Size: 1255 2082 17 153 185 445 1498
Fragments arranged by size:
2082 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2184 2408 2509
Size: 274 80 33 51 1443 207 224 101
Cuts at: 2509 3881 5593 96
Size: 1372 1712 138
Fragments arranged by size:
1712 1443 1372 274 224 207 138 101
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2543 2575 5595 5627 2543
Size: 32 3020 32 2551
Fragments arranged by size:
3020 2551 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5635
XbaI T'CTAG_A
Cuts at: 2971 2971
Size: 5635
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2371 2723 2371
Size: 352 5283
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5635
XmaI C'CCGG_G
Cuts at: 1870 2960 1870
Size: 1090 4545
XmnI GAAnn'nnTTC
Cuts at: 3764 3764
Size: 5635
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3443 276
Size: 53 83 186 936 1909 2468
Fragments arranged by size:
2468 1909 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I
EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SrfI SwaI