Restriction map for
pFN21AB8338
(Circular) (Six-base) MAPSORT of: pFN21AB8338.seq Check: 2548 from: 1 to: 5521
pFN21AB8338 5521 bp
With 182 enzymes: SgfI *
February 27, 2009 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3331 278
Size: 53 83 186 936 1795 2468
Fragments arranged by size:
2468 1795 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2864 1937
Size: 927 4594
Acc65I G'GTAC_C
Cuts at: 2842 2842
Size: 5521
AclI AA'CG_TT
Cuts at: 1563 3648 4021 1563
Size: 2085 373 3063
Fragments arranged by size:
3063 2085 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2140 3584 4968 5236 1632
Size: 508 1444 1384 268 1917
Fragments arranged by size:
1917 1444 1384 508 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5324
AflIII A'CryG_T
Cuts at: 1203 2688 4420 5397 1203
Size: 1485 1732 977 1327
Fragments arranged by size:
1732 1485 1327 977
AgeI A'CCGG_T
Cuts at: 4352 4352
Size: 5521
AhdI GACnn_n'nnGTC
Cuts at: 4250 4250
Size: 5521
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5521
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2342 1184
Size: 1158 4363
AlwNI CAG_nnn'CTG
Cuts at: 2659 3264 4836 2659
Size: 605 1572 3344
Fragments arranged by size:
3344 1572 605
ApaI G_GGCC'C
Cuts at: 2023 2023
Size: 5521
ApaLI G'TGCA_C
Cuts at: 2213 2279 3578 4734 2213
Size: 66 1299 1156 3000
Fragments arranged by size:
3000 1299 1156 66
ApoI r'AATT_y
Cuts at: 1493 2830 3056 3208 5317 1493
Size: 1337 226 152 2109 1697
Fragments arranged by size:
2109 1697 1337 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4075 160
Size: 872 3043 1606
Fragments arranged by size:
3043 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5521
AvaI C'yCGr_G
Cuts at: 1870 1943 2846 1870
Size: 73 903 4545
Fragments arranged by size:
4545 903 73
BamHI G'GATC_C
Cuts at: 2851 3218 2851
Size: 367 5154
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2057 2195 2225 2290 2308
Size: 325 217 627 270 138 30 65 18
Cuts at: 2308 2522 2634 2842 4297 618
Size: 214 112 208 1455 1842
Fragments arranged by size:
1842 1455 627 325 270 217 214 208
138 112 65 30 18
BanII G_rGCy'C
Cuts at: 1391 2023 2346 2840 1391
Size: 632 323 494 4072
Fragments arranged by size:
4072 632 494 323
BbeI G_GCGC'C
Cuts at: 2061 2199 2229 2294 2312 2526 2061
Size: 138 30 65 18 214 5056
Fragments arranged by size:
5056 214 138 65 30 18
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4550
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3712 3746 1903
Size: 34 1775 34 3678
Fragments arranged by size:
3678 1775 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3413 4623 3413
Size: 1210 4311
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5521
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2122 4132 136
Size: 107 122 71 631 605 450 2010 1525
Fragments arranged by size:
2010 1525 631 605 450 122 107 71
BglII A'GATC_T
Cuts at: 2757 5516 2757
Size: 2759 2762
BlpI GC'TnA_GC
Cuts at: 2078 2938 2078
Size: 860 4661
Bme1580I G_kGCm'C
Cuts at: 1790 2023 2217 2283 2639 3582 4738 1790
Size: 233 194 66 356 943 1156 2573
Fragments arranged by size:
2573 1156 943 356 233 194 66
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5521
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2025 2204 3252
Size: 416 53 83 399 24 608 179 1048
Cuts at: 3252 4210 442
Size: 958 1753
Fragments arranged by size:
1753 1048 958 608 416 399 179 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5521
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2360 2365 2475 2687 4181 1239
Size: 1121 5 110 212 1494 2579
Fragments arranged by size:
2579 1494 1121 212 110 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3646 4511 4809 3646
Size: 865 298 4358
Fragments arranged by size:
4358 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4184 882
Size: 617 2685 2219
Fragments arranged by size:
2685 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2038 2278 3312 493
Size: 711 834 240 1034 2702
Fragments arranged by size:
2702 1034 834 711 240
BsaBI GATnn'nnATC
Cuts at: 5515 5515
Size: 5521
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2058 2064 2196 2226
Size: 53 83 186 936 525 6 132 30
Cuts at: 2226 2291 2309 2523 3328 3710 4345 275
Size: 65 18 214 805 382 635 1451
Fragments arranged by size:
1451 936 805 635 525 382 214 186
132 83 65 53 30 18 6
BsaWI w'CCGG_w
Cuts at: 3954 4352 4626 4773 5184 3954
Size: 398 274 147 411 4291
Fragments arranged by size:
4291 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2315 2345 2660 2690 1178
Size: 30 165 30 912 30 315 30 4009
Fragments arranged by size:
4009 912 315 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2478 1186
Size: 1292 4229
BseYI C'CCAG_C
Cuts at: 2053 2513 2590 4724 5220 2053
Size: 460 77 2134 496 2354
Fragments arranged by size:
2354 2134 496 460 77
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2185 1163
Size: 573 449 4499
Fragments arranged by size:
4499 573 449
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2971 3732 4760 1540
Size: 275 186 970 761 1028 2301
Fragments arranged by size:
2301 1028 970 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2148 2217 2283 3582 3667 4738 2148
Size: 69 66 1299 85 1071 2931
Fragments arranged by size:
2931 1299 1071 85 69 66
BsmI GAATG_Cn'
Cuts at: 3032 3125 3032
Size: 93 5428
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2023 2148 2217 2283 2346 2639 2840
Size: 399 233 125 69 66 63 293 201
Cuts at: 2840 3582 3667 4738 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 293 233 201 125
85 69 66 63
BspEI T'CCGG_A
Cuts at: 5184 5184
Size: 5521
BspHI T'CATG_A
Cuts at: 3410 5140 3410
Size: 1730 3791
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2310 2700 2740 2876 844
Size: 1466 390 40 136 3489
Fragments arranged by size:
3489 1466 390 136 40
BsrBI CCG'CTC
Cuts at: 1394 1823 2556 3408 1394
Size: 429 733 852 3507
Fragments arranged by size:
3507 852 733 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4016 4190 65
Size: 1183 2768 174 1396
Fragments arranged by size:
2768 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2385 4165 4352 1700
Size: 110 575 1780 187 2869
Fragments arranged by size:
2869 1780 575 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5521
BssHII G'CGCG_C
Cuts at: 1925 2612 1925
Size: 687 4834
BssSI C'ACGA_G
Cuts at: 2200 3581 4593 2200
Size: 1381 1012 3128
Fragments arranged by size:
3128 1381 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5521
BstBI TT'CG_AA
Cuts at: 4325 4325
Size: 5521
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5521
BstYI r'GATC_y
Cuts at: 1972 2757 2851 3218 3603 3620 5072 5158 5170
Size: 785 94 367 385 17 1452 86 12
Cuts at: 5170 5516 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 785 385 367 346 94 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2067 2295 513
Size: 552 939 63 228 3739
Fragments arranged by size:
3739 939 552 228 63
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4782
Fragments arranged by size:
4782 683 56
BtsI GCAGTG_nn'
Cuts at: 3037 3831 3851 3037
Size: 794 20 4707
Fragments arranged by size:
4707 794 20
ClaI AT'CG_AT
Cuts at: 1554 2577 1554
Size: 1023 4498
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5486
DraI TTT'AAA
Cuts at: 2825 2825
Size: 5521
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4528 809
Size: 507 3212 1802
Fragments arranged by size:
3212 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2086 2139
Size: 54 1425 50 275 18 177 79 53
Cuts at: 2139 2418 2968 3857 5486 8
Size: 279 550 889 1629 43
Fragments arranged by size:
1629 1425 889 550 279 275 177 79
54 53 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2968 1537
Size: 275 1156 4090
Fragments arranged by size:
4090 1156 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3451 1353
Size: 2098 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2574 4108 4482 4628 387
Size: 1064 138 985 1534 374 146 1280
Fragments arranged by size:
1534 1280 1064 985 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2140 2360 2365 2475 2687 3584 4181
Size: 393 508 220 5 110 212 897 597
Cuts at: 4181 4968 5236 1239
Size: 787 268 1524
Fragments arranged by size:
1524 897 787 597 508 393 268 220
212 110 5
EcoO109I rG'GnC_Cy
Cuts at: 2019 2020 2103 2019
Size: 1 83 5437
Fragments arranged by size:
5437 83 1
EcoRI G'AATT_C
Cuts at: 2830 3208 2830
Size: 378 5143
FspI TGC'GCA
Cuts at: 4027 4027
Size: 5521
HaeII r_GCGC'y
Cuts at: 1678 2061 2199 2229 2294 2312 2526 4668 1678
Size: 383 138 30 65 18 214 2142 2531
Fragments arranged by size:
2531 2142 383 214 138 65 30 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4162 4194 4236 4268 1373
Size: 32 135 32 2590 32 42 32 2626
Fragments arranged by size:
2626 2590 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2865 3111 669
Size: 988 281 927 246 3079
Fragments arranged by size:
3079 988 927 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5521
HpaI GTT'AAC
Cuts at: 3111 3111
Size: 5521
KasI G'GCGC_C
Cuts at: 2057 2195 2225 2290 2308 2522 2057
Size: 138 30 65 18 214 5056
Fragments arranged by size:
5056 214 138 65 30 18
KpnI G_GTAC'C
Cuts at: 2846 2846
Size: 5521
MfeI C'AATT_G
Cuts at: 3120 3120
Size: 5521
MluI A'CGCG_T
Cuts at: 5397 5397
Size: 5521
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4635 4819 1305
Size: 3330 184 2007
Fragments arranged by size:
3330 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 10
Size: 54 1425 343 177 3522
Fragments arranged by size:
3522 1425 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3479 3838 3997 518
Size: 648 2313 359 159 2042
Fragments arranged by size:
2313 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2069 2243 2413 2937 3614 4762 5007 1606
Size: 463 174 170 524 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 524 463 245 174 170
NaeI GCC'GGC
Cuts at: 2387 2387
Size: 5521
NarI GG'CG_CC
Cuts at: 2058 2196 2226 2291 2309 2523 2058
Size: 138 30 65 18 214 5056
Fragments arranged by size:
5056 214 138 65 30 18
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4030
Fragments arranged by size:
4030 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5521
NgoMIV G'CCGG_C
Cuts at: 2385 2385
Size: 5521
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5521
NotI GC'GGCC_GC
Cuts at: 2968 2968
Size: 5521
NspI r_CATG'y
Cuts at: 1132 1229 2329 2692 2879 4424 1132
Size: 97 1100 363 187 1545 2229
Fragments arranged by size:
2229 1545 1100 363 187 97
PasI CC'CwG_GG
Cuts at: 1348 2023 2107 1348
Size: 675 84 4762
Fragments arranged by size:
4762 675 84
PciI A'CATG_T
Cuts at: 2688 4420 2688
Size: 1732 3789
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2659 1108
Size: 1551 3970
PmeI GTTT'AAAC
Cuts at: 2825 2825
Size: 5521
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2666 2698 3590 3622 5103 5135 2666
Size: 32 892 32 1481 32 3052
Fragments arranged by size:
3052 1481 892 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2103 2103
Size: 5521
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5521
PsiI TTA'TAA
Cuts at: 3091 3091
Size: 5521
PspOMI G'GGCC_C
Cuts at: 2019 2019
Size: 5521
PstI C_TGCA'G
Cuts at: 830 2873 830
Size: 2043 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5521
SacII CC_GC'GG
Cuts at: 2070 2070
Size: 5521
SalI G'TCGA_C
Cuts at: 1936 2863 1936
Size: 927 4594
SanDI GG'GwC_CC
Cuts at: 2103 2103
Size: 5521
SbfI CC_TGCA'GG
Cuts at: 2873 2873
Size: 5521
ScaI AGT'ACT
Cuts at: 3769 5481 3769
Size: 1712 3809
SfcI C'TryA_G
Cuts at: 826 1045 2869 4004 4685 4876 826
Size: 219 1824 1135 681 191 1471
Fragments arranged by size:
1824 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2059 2197 2227 2292 2310 2524 2059
Size: 138 30 65 18 214 5056
Fragments arranged by size:
5056 214 138 65 30 18
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5521
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5521
SmaI CCC'GGG
Cuts at: 1872 2848 1872
Size: 976 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3625 4526 4788 820
Size: 197 926 1682 901 262 1553
Fragments arranged by size:
1682 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3312 493
Size: 2819 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5521
SphI G_CATG'C
Cuts at: 1132 2879 1132
Size: 1747 3774
SspI AAT'ATT
Cuts at: 5 52 3445 5
Size: 47 3393 2081
Fragments arranged by size:
3393 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5521
StyI C'CwwG_G
Cuts at: 513 1065 2004 2178 2960 513
Size: 552 939 174 782 3074
Fragments arranged by size:
3074 939 782 552 174
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2820 3548 3565 3718 3903 4348 325
Size: 1255 1240 728 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1240 728 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3767 5479 96
Size: 274 80 33 51 1443 1790 1712 138
Fragments arranged by size:
1790 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2444 2476 5481 5513 2444
Size: 32 3005 32 2452
Fragments arranged by size:
3005 2452 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5521
XbaI T'CTAG_A
Cuts at: 2857 2857
Size: 5521
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2020 2598 2020
Size: 578 4943
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5521
XmaI C'CCGG_G
Cuts at: 1870 2846 1870
Size: 976 4545
XmnI GAAnn'nnTTC
Cuts at: 3650 3650
Size: 5521
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3329 276
Size: 53 83 186 936 1795 2468
Fragments arranged by size:
2468 1795 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI SacII SalI SanDI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI
PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI
SapI SexAI SfiI SrfI SwaI