Restriction map for
pFN21AB8337
(Circular) (Six-base) MAPSORT of: pFN21AB8337.seq Check: 911 from: 1 to: 6322
pFN21AB8337 6322 bp
With 182 enzymes: SgfI *
February 27, 2009 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4132 278
Size: 53 83 186 936 2596 2468
Fragments arranged by size:
2596 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3665 1937
Size: 1728 4594
Acc65I G'GTAC_C
Cuts at: 3643 3643
Size: 6322
AclI AA'CG_TT
Cuts at: 1563 4449 4822 1563
Size: 2886 373 3063
Fragments arranged by size:
3063 2886 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2620 2784 2788 3040 3460 4385 5769 6037
Size: 988 164 4 252 420 925 1384 268
Cuts at: 6037 1632
Size: 1917
Fragments arranged by size:
1917 1384 988 925 420 268 252 164
4
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6125
AflIII A'CryG_T
Cuts at: 1203 3576 5221 6198 1203
Size: 2373 1645 977 1327
Fragments arranged by size:
2373 1645 1327 977
AgeI A'CCGG_T
Cuts at: 2862 5153 2862
Size: 2291 4031
AhdI GACnn_n'nnGTC
Cuts at: 5051 5051
Size: 6322
AleI CACnn'nnGTG
Cuts at: 1166 2529 1166
Size: 1363 4959
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6322
AlwNI CAG_nnn'CTG
Cuts at: 2706 3427 4065 5637 2706
Size: 721 638 1572 3391
Fragments arranged by size:
3391 1572 721 638
ApaI G_GGCC'C
Cuts at: 2102 2102
Size: 6322
ApaLI G'TGCA_C
Cuts at: 4379 5535 4379
Size: 1156 5166
ApoI r'AATT_y
Cuts at: 1493 2670 3174 3631 3857 4009 6118 1493
Size: 1177 504 457 226 152 2109 1697
Fragments arranged by size:
2109 1697 1177 504 457 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4876 160
Size: 872 3844 1606
Fragments arranged by size:
3844 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6322
AvaI C'yCGr_G
Cuts at: 1870 1943 2076 3647 1870
Size: 73 133 1571 4545
Fragments arranged by size:
4545 1571 133 73
BamHI G'GATC_C
Cuts at: 3510 3652 4019 3510
Size: 142 367 5813
Fragments arranged by size:
5813 367 142
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2246 3643 5098 618
Size: 325 217 627 459 1397 1455 1842
Fragments arranged by size:
1842 1455 1397 627 459 325 217
BanII G_rGCy'C
Cuts at: 1391 2102 2226 3641 1391
Size: 711 124 1415 4072
Fragments arranged by size:
4072 1415 711 124
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2888 928
Size: 971 989 4362
Fragments arranged by size:
4362 989 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4513 4547 1903
Size: 34 2576 34 3678
Fragments arranged by size:
3678 2576 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4214 5424 4214
Size: 1210 5112
BclI T'GATC_A
Cuts at: 1548 2512 1548
Size: 964 5358
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2097 4933
Size: 107 122 71 631 605 337 88 2836
Cuts at: 4933 136
Size: 1525
Fragments arranged by size:
2836 1525 631 605 337 122 107 88
71
BglII A'GATC_T
Cuts at: 6317 6317
Size: 6322
BlpI GC'TnA_GC
Cuts at: 3739 3739
Size: 6322
Bme1580I G_kGCm'C
Cuts at: 1790 2102 2249 4383 5539 1790
Size: 312 147 2134 1156 2573
Fragments arranged by size:
2573 2134 1156 312 147
BmgBI CAC'GTC
Cuts at: 1318 2059 2128 1318
Size: 741 69 5512
Fragments arranged by size:
5512 741 69
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2171 2688 2940
Size: 416 53 83 399 24 754 517 252
Cuts at: 2940 4053 5011 442
Size: 1113 958 1753
Fragments arranged by size:
1753 1113 958 754 517 416 399 252
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6322
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3631 3631
Size: 6322
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2156 2211 3204 3288 4982 1239
Size: 917 55 993 84 1694 2579
Fragments arranged by size:
2579 1694 993 917 84 55
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4447 5312 5610 4447
Size: 865 298 5159
Fragments arranged by size:
5159 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2132 2512 4985 882
Size: 617 633 380 2473 2219
Fragments arranged by size:
2473 2219 633 617 380
BsaAI yAC'GTr
Cuts at: 493 1204 3489 4113 493
Size: 711 2285 624 2702
Fragments arranged by size:
2702 2285 711 624
BsaBI GATnn'nnATC
Cuts at: 6316 6316
Size: 6322
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4129 4511 5146 275
Size: 53 83 186 936 2596 382 635 1451
Fragments arranged by size:
2596 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2862 3014 3098 3504 4755 5153 5427 5574 5985
Size: 152 84 406 1251 398 274 147 411
Cuts at: 5985 2862
Size: 3199
Fragments arranged by size:
3199 1251 411 406 398 274 152 147
84
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6097
Fragments arranged by size:
6097 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6322
BseYI C'CCAG_C
Cuts at: 2281 5525 6021 2281
Size: 3244 496 2582
Fragments arranged by size:
3244 2582 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3391 3413 3559 1163
Size: 573 1655 22 146 3926
Fragments arranged by size:
3926 1655 573 146 22
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3772 4533 5561 1540
Size: 275 186 1771 761 1028 2301
Fragments arranged by size:
2301 1771 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2265 3488 4383 4468 5539 2265
Size: 1223 895 85 1071 3048
Fragments arranged by size:
3048 1223 1071 895 85
BsmI GAATG_Cn'
Cuts at: 3290 3833 3926 3290
Size: 543 93 5686
Fragments arranged by size:
5686 543 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2102 2226 2249 2265 3488 3641 4383
Size: 399 312 124 23 16 1223 153 742
Cuts at: 4383 4468 5539 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1223 1071 742 399 312 153 124
85 23 16
BspEI T'CCGG_A
Cuts at: 5985 5985
Size: 6322
BspHI T'CATG_A
Cuts at: 2907 4211 5941 2907
Size: 1304 1730 3288
Fragments arranged by size:
3288 1730 1304
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2182 3677 844
Size: 1338 1495 3489
Fragments arranged by size:
3489 1495 1338
BsrBI CCG'CTC
Cuts at: 1394 1823 4209 1394
Size: 429 2386 3507
Fragments arranged by size:
3507 2386 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3295 4817 4991 65
Size: 1183 2047 1522 174 1396
Fragments arranged by size:
2047 1522 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2862 4966 5153 1700
Size: 110 1052 2104 187 2869
Fragments arranged by size:
2869 2104 1052 187 110
BsrGI T'GTAC_A
Cuts at: 96 2587 96
Size: 2491 3831
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6322
BssSI C'ACGA_G
Cuts at: 3242 4382 5394 3242
Size: 1140 1012 4170
Fragments arranged by size:
4170 1140 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3462 794
Size: 2668 3654
BstBI TT'CG_AA
Cuts at: 5126 5126
Size: 6322
BstEII G'GTnAC_C
Cuts at: 1184 2044 1184
Size: 860 5462
BstXI CCAn_nnnn'nTGG
Cuts at: 3244 3244
Size: 6322
BstYI r'GATC_y
Cuts at: 1972 2344 2354 3510 3652 4019 4404 4421 5873
Size: 372 10 1156 142 367 385 17 1452
Cuts at: 5873 5959 5971 6317 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1156 385 372 367 346 142
86 17 12 10
Bsu36I CC'TnA_GG
Cuts at: 2486 2486
Size: 6322
BtgI C'CryG_G
Cuts at: 513 1065 2004 3433 3618 513
Size: 552 939 1429 185 3217
Fragments arranged by size:
3217 1429 939 552 185
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5583
Fragments arranged by size:
5583 683 56
BtsI GCAGTG_nn'
Cuts at: 3838 4632 4652 3838
Size: 794 20 5508
Fragments arranged by size:
5508 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6322
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3109 3144 3277 3312 529
Size: 35 2545 35 133 35 3539
Fragments arranged by size:
3539 2545 133 35 35 35
DraI TTT'AAA
Cuts at: 3626 3626
Size: 6322
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5329 809
Size: 507 4013 1802
Fragments arranged by size:
4013 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3769 4658 6287
Size: 54 1425 50 275 18 1939 889 1629
Cuts at: 6287 8
Size: 43
Fragments arranged by size:
1939 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3769 1537
Size: 275 1957 4090
Fragments arranged by size:
4090 1957 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2760 2989 4252 1353
Size: 1407 229 1263 3423
Fragments arranged by size:
3423 1407 1263 229
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4909 5283 5429 387
Size: 1064 138 3320 374 146 1280
Fragments arranged by size:
3320 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2156 2211 2620 2784 2788 3040 3204
Size: 393 524 55 409 164 4 252 164
Cuts at: 3204 3288 3460 4385 4982 5769 6037 1239
Size: 84 172 925 597 787 268 1524
Fragments arranged by size:
1524 925 787 597 524 409 393 268
252 172 164 164 84 55 4
EcoNI CCTnn'n_nnAGG
Cuts at: 3605 3605
Size: 6322
EcoO109I rG'GnC_Cy
Cuts at: 2099 2113 2171 2180 2230 2429 2099
Size: 14 58 9 50 199 5992
Fragments arranged by size:
5992 199 58 50 14 9
EcoRI G'AATT_C
Cuts at: 3174 3631 4009 3174
Size: 457 378 5487
Fragments arranged by size:
5487 457 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3490 3490
Size: 6322
FspI TGC'GCA
Cuts at: 4828 4828
Size: 6322
HaeII r_GCGC'y
Cuts at: 1678 2979 5469 1678
Size: 1301 2490 2531
Fragments arranged by size:
2531 2490 1301
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2551 2553 2583 2585 4963
Size: 32 135 32 979 2 30 2 2378
Cuts at: 4963 4995 5037 5069 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2378 979 135 42 32 32 32
32 30 2 2
HincII GTy'rAC
Cuts at: 669 1657 1938 2275 2316 3666 3912 669
Size: 988 281 337 41 1350 246 3079
Fragments arranged by size:
3079 1350 988 337 281 246 41
HindIII A'AGCT_T
Cuts at: 748 3219 748
Size: 2471 3851
HpaI GTT'AAC
Cuts at: 3912 3912
Size: 6322
KpnI G_GTAC'C
Cuts at: 3647 3647
Size: 6322
MfeI C'AATT_G
Cuts at: 3921 3921
Size: 6322
MluI A'CGCG_T
Cuts at: 6198 6198
Size: 6322
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2187 3068 3103 3187 3597 5436 5620 1305
Size: 882 881 35 84 410 1839 184 2007
Fragments arranged by size:
2007 1839 882 881 410 184 84 35
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4500
Fragments arranged by size:
4500 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2529 2583 3115 4280 4639 4798 518
Size: 648 1363 54 532 1165 359 159 2042
Fragments arranged by size:
2042 1363 1165 648 532 359 159 54
MspA1I CmG'CkG
Cuts at: 1606 2752 3592 3738 4415 5563 5808 1606
Size: 1146 840 146 677 1148 245 2120
Fragments arranged by size:
2120 1148 1146 840 677 245 146
NcoI C'CATG_G
Cuts at: 513 1065 2004 3618 513
Size: 552 939 1614 3217
Fragments arranged by size:
3217 1614 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6322
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6322
NotI GC'GGCC_GC
Cuts at: 3769 3769
Size: 6322
NspI r_CATG'y
Cuts at: 1132 1229 3580 3680 5225 1132
Size: 97 2351 100 1545 2229
Fragments arranged by size:
2351 2229 1545 100 97
PasI CC'CwG_GG
Cuts at: 1348 2226 2350 2433 1348
Size: 878 124 83 5237
Fragments arranged by size:
5237 878 124 83
PciI A'CATG_T
Cuts at: 3576 5221 3576
Size: 1645 4677
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2288 1108
Size: 1180 5142
PfoI T'CCnGG_A
Cuts at: 2232 3603 2232
Size: 1371 4951
PmeI GTTT'AAAC
Cuts at: 3626 3626
Size: 6322
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2141 2173 4391 4423 5904 5936 2141
Size: 32 2218 32 1481 32 2527
Fragments arranged by size:
2527 2218 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2171 2230 2171
Size: 59 6263
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6322
PsiI TTA'TAA
Cuts at: 3892 3892
Size: 6322
PspOMI G'GGCC_C
Cuts at: 2098 2098
Size: 6322
PstI C_TGCA'G
Cuts at: 830 2179 3674 830
Size: 1349 1495 3478
Fragments arranged by size:
3478 1495 1349
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6322
SalI G'TCGA_C
Cuts at: 1936 3664 1936
Size: 1728 4594
SbfI CC_TGCA'GG
Cuts at: 2179 3674 2179
Size: 1495 4827
ScaI AGT'ACT
Cuts at: 4570 6282 4570
Size: 1712 4610
SfcI C'TryA_G
Cuts at: 826 1045 2119 2175 3448 3670 4805 5486 5677
Size: 219 1074 56 1273 222 1135 681 191
Cuts at: 5677 826
Size: 1471
Fragments arranged by size:
1471 1273 1135 1074 681 222 219 191
56
SfiI GGCCn_nnn'nGGCC
Cuts at: 2097 2097
Size: 6322
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6322
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6322
SmaI CCC'GGG
Cuts at: 1872 2078 3649 1872
Size: 206 1571 4545
Fragments arranged by size:
4545 1571 206
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4426 5327 5589 820
Size: 197 926 2483 901 262 1553
Fragments arranged by size:
2483 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4113 493
Size: 3620 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6322
SphI G_CATG'C
Cuts at: 1132 3680 1132
Size: 2548 3774
SspI AAT'ATT
Cuts at: 5 52 4246 5
Size: 47 4194 2081
Fragments arranged by size:
4194 2081 47
StuI AGG'CCT
Cuts at: 1517 3389 3557 1517
Size: 1872 168 4282
Fragments arranged by size:
4282 1872 168
StyI C'CwwG_G
Cuts at: 513 1065 2004 3618 3761 513
Size: 552 939 1614 143 3074
Fragments arranged by size:
3074 1614 939 552 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4349 4366 4519 4704 5149 325
Size: 1255 2769 17 153 185 445 1498
Fragments arranged by size:
2769 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2304 2587 4568
Size: 274 80 33 51 1443 327 283 1981
Cuts at: 4568 6280 96
Size: 1712 138
Fragments arranged by size:
1981 1712 1443 327 283 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3312 3344 3386 3418 6282 6314 3312
Size: 32 42 32 2864 32 3320
Fragments arranged by size:
3320 2864 42 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3084 1316
Size: 1768 4554
XbaI T'CTAG_A
Cuts at: 3658 3658
Size: 6322
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6322
XmaI C'CCGG_G
Cuts at: 1870 2076 3647 1870
Size: 206 1571 4545
Fragments arranged by size:
4545 1571 206
XmnI GAAnn'nnTTC
Cuts at: 2717 2801 2885 3053 3221 3389 3473 3557 4451
Size: 84 84 168 168 168 84 84 894
Cuts at: 4451 2717
Size: 4588
Fragments arranged by size:
4588 894 168 168 168 84 84 84
84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4130 276
Size: 53 83 186 936 2596 2468
Fragments arranged by size:
2596 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfoI SrfI SwaI XcmI