Restriction map for
pFN21AB8335
(Circular) (Six-base) MAPSORT of: pFN21AB8335.seq Check: 4589 from: 1 to: 6025
pFN21AB8335 6025 bp
With 182 enzymes: SgfI *
February 27, 2009 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3835 278
Size: 53 83 186 936 2299 2468
Fragments arranged by size:
2468 2299 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2418 3368 1937
Size: 481 950 4594
Fragments arranged by size:
4594 950 481
Acc65I G'GTAC_C
Cuts at: 3346 3346
Size: 6025
AclI AA'CG_TT
Cuts at: 1563 4152 4525 1563
Size: 2589 373 3063
Fragments arranged by size:
3063 2589 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2061 2579 4088 5472 5740 1632
Size: 429 518 1509 1384 268 1917
Fragments arranged by size:
1917 1509 1384 518 429 268
AfeI AGC'GCT
Cuts at: 3215 3215
Size: 6025
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5828
AflIII A'CryG_T
Cuts at: 1203 4924 5901 1203
Size: 3721 977 1327
Fragments arranged by size:
3721 1327 977
AgeI A'CCGG_T
Cuts at: 4856 4856
Size: 6025
AhdI GACnn_n'nnGTC
Cuts at: 4754 4754
Size: 6025
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6025
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2525 3075 1184
Size: 1341 550 4134
Fragments arranged by size:
4134 1341 550
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2348 2380 2348
Size: 32 5993
AlwNI CAG_nnn'CTG
Cuts at: 2185 2518 3106 3768 5340 2185
Size: 333 588 662 1572 2870
Fragments arranged by size:
2870 1572 662 588 333
ApaLI G'TGCA_C
Cuts at: 2324 4082 5238 2324
Size: 1758 1156 3111
Fragments arranged by size:
3111 1758 1156
ApoI r'AATT_y
Cuts at: 1493 2543 3334 3560 3712 5821 1493
Size: 1050 791 226 152 2109 1697
Fragments arranged by size:
2109 1697 1050 791 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4579 160
Size: 872 3547 1606
Fragments arranged by size:
3547 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6025
AvaI C'yCGr_G
Cuts at: 1870 1943 2030 3350 1870
Size: 73 87 1320 4545
Fragments arranged by size:
4545 1320 87 73
BamHI G'GATC_C
Cuts at: 2489 3355 3722 2489
Size: 866 367 4792
Fragments arranged by size:
4792 866 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3346 4801 618
Size: 325 217 627 1559 1455 1842
Fragments arranged by size:
1842 1559 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2090 2175 3344 1391
Size: 699 85 1169 4072
Fragments arranged by size:
4072 1169 699 85
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2403 928
Size: 971 504 4550
Fragments arranged by size:
4550 971 504
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4216 4250 1903
Size: 34 2279 34 3678
Fragments arranged by size:
3678 2279 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3917 5127 3917
Size: 1210 4815
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6025
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4636 136
Size: 107 122 71 631 605 2964 1525
Fragments arranged by size:
2964 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6020 6020
Size: 6025
BlpI GC'TnA_GC
Cuts at: 2633 2768 2816 3442 2633
Size: 135 48 626 5216
Fragments arranged by size:
5216 626 135 48
Bme1580I G_kGCm'C
Cuts at: 1790 2328 4086 5242 1790
Size: 538 1758 1156 2573
Fragments arranged by size:
2573 1758 1156 538
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6025
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3280 3756 4714
Size: 416 53 83 399 24 1863 476 958
Cuts at: 4714 442
Size: 1753
Fragments arranged by size:
1863 1753 958 476 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6025
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2104 2104
Size: 6025
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2141 2693 2707 3047 3059 3153 4685 1239
Size: 902 552 14 340 12 94 1532 2579
Fragments arranged by size:
2579 1532 902 552 340 94 14 12
Bpu10I CC'TnA_GC
Cuts at: 2829 2829
Size: 6025
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2641 2858 4150 5015 5313 2641
Size: 217 1292 865 298 3353
Fragments arranged by size:
3353 1292 865 298 217
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2004 3128 4688 882
Size: 617 505 1124 1560 2219
Fragments arranged by size:
2219 1560 1124 617 505
BsaAI yAC'GTr
Cuts at: 493 1204 3816 493
Size: 711 2612 2702
Fragments arranged by size:
2702 2612 711
BsaBI GATnn'nnATC
Cuts at: 6019 6019
Size: 6025
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3832 4214 4849 275
Size: 53 83 186 936 2299 382 635 1451
Fragments arranged by size:
2299 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4458 4856 5130 5277 5688 4458
Size: 398 274 147 411 4795
Fragments arranged by size:
4795 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2321 2348 2351 2378 2959
Size: 30 165 30 918 27 3 27 581
Cuts at: 2959 2989 1178
Size: 30 4214
Fragments arranged by size:
4214 918 581 165 30 30 30 27
27 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2237 1186
Size: 1051 4974
BseYI C'CCAG_C
Cuts at: 2970 5228 5724 2970
Size: 2258 496 3271
Fragments arranged by size:
3271 2258 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3219 1163
Size: 573 1483 3969
Fragments arranged by size:
3969 1483 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3475 4236 5264 1540
Size: 275 186 1474 761 1028 2301
Fragments arranged by size:
2301 1474 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2328 4086 4171 5242 2328
Size: 1758 85 1071 3111
Fragments arranged by size:
3111 1758 1071 85
BsmI GAATG_Cn'
Cuts at: 3536 3629 3536
Size: 93 5932
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2090 2175 2328 3344 4086 4171 5242
Size: 399 300 85 153 1016 742 85 1071
Cuts at: 5242 1391
Size: 2174
Fragments arranged by size:
2174 1071 1016 742 399 300 153 85
85
BspEI T'CCGG_A
Cuts at: 5688 5688
Size: 6025
BspHI T'CATG_A
Cuts at: 3914 5644 3914
Size: 1730 4295
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2655 2817 3114 3380 844
Size: 1811 162 297 266 3489
Fragments arranged by size:
3489 1811 297 266 162
BsrBI CCG'CTC
Cuts at: 1394 1823 3022 3175 3912 1394
Size: 429 1199 153 737 3507
Fragments arranged by size:
3507 1199 737 429 153
BsrDI GCAATG_nn'
Cuts at: 65 1248 4520 4694 65
Size: 1183 3272 174 1396
Fragments arranged by size:
3272 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2691 4669 4856 1700
Size: 110 881 1978 187 2869
Fragments arranged by size:
2869 1978 881 187 110
BsrGI T'GTAC_A
Cuts at: 96 2802 96
Size: 2706 3319
BssHII G'CGCG_C
Cuts at: 1925 2834 1925
Size: 909 5116
BssSI C'ACGA_G
Cuts at: 2330 4085 5097 2330
Size: 1755 1012 3258
Fragments arranged by size:
3258 1755 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2518 3106 3255 794
Size: 1724 588 149 3564
Fragments arranged by size:
3564 1724 588 149
BstBI TT'CG_AA
Cuts at: 4829 4829
Size: 6025
BstEII G'GTnAC_C
Cuts at: 1184 2191 1184
Size: 1007 5018
BstXI CCAn_nnnn'nTGG
Cuts at: 2317 3015 2317
Size: 698 5327
BstYI r'GATC_y
Cuts at: 1972 2489 3003 3355 3722 4107 4124 5576 5662
Size: 517 514 352 367 385 17 1452 86
Cuts at: 5662 5674 6020 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 517 514 385 367 352 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2478 2823 2478
Size: 345 5680
BtgI C'CryG_G
Cuts at: 513 1065 2004 2197 513
Size: 552 939 193 4341
Fragments arranged by size:
4341 939 552 193
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5286
Fragments arranged by size:
5286 683 56
BtsI GCAGTG_nn'
Cuts at: 2241 2900 3168 3202 3541 4335 4355 2241
Size: 659 268 34 339 794 20 3911
Fragments arranged by size:
3911 794 659 339 268 34 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6025
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2199 2234 529
Size: 35 1635 35 4320
Fragments arranged by size:
4320 1635 35 35
DraI TTT'AAA
Cuts at: 3329 3329
Size: 6025
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5032 809
Size: 507 3716 1802
Fragments arranged by size:
3716 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3472 4361 5990
Size: 54 1425 50 275 18 1642 889 1629
Cuts at: 5990 8
Size: 43
Fragments arranged by size:
1642 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3472 1537
Size: 275 1660 4090
Fragments arranged by size:
4090 1660 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3955 1353
Size: 2602 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4612 4986 5132 387
Size: 1064 138 3023 374 146 1280
Fragments arranged by size:
3023 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2061 2141 2579 2693 2707 3047 3059
Size: 393 429 80 438 114 14 340 12
Cuts at: 3059 3153 4088 4685 5472 5740 1239
Size: 94 935 597 787 268 1524
Fragments arranged by size:
1524 935 787 597 438 429 393 340
268 114 94 80 14 12
EcoO109I rG'GnC_Cy
Cuts at: 2206 2563 2986 3103 2206
Size: 357 423 117 5128
Fragments arranged by size:
5128 423 357 117
EcoRI G'AATT_C
Cuts at: 3334 3712 3334
Size: 378 5647
FspI TGC'GCA
Cuts at: 2063 4531 2063
Size: 2468 3557
HaeII r_GCGC'y
Cuts at: 1678 3217 5172 1678
Size: 1539 1955 2531
Fragments arranged by size:
2531 1955 1539
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2591 2623 2856 2888 4666
Size: 32 135 32 1019 32 233 32 1778
Cuts at: 4666 4698 4740 4772 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1778 1019 233 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3369 3615 669
Size: 988 281 1431 246 3079
Fragments arranged by size:
3079 1431 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2876 748
Size: 2128 3897
HpaI GTT'AAC
Cuts at: 3615 3615
Size: 6025
KpnI G_GTAC'C
Cuts at: 3350 3350
Size: 6025
MfeI C'AATT_G
Cuts at: 3057 3624 3057
Size: 567 5458
MluI A'CGCG_T
Cuts at: 5901 5901
Size: 6025
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2757 2976 5139 5323 1305
Size: 1452 219 2163 184 2007
Fragments arranged by size:
2163 2007 1452 219 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4203
Fragments arranged by size:
4203 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2315 2321 3983 4342 4501 518
Size: 648 1149 6 1662 359 159 2042
Fragments arranged by size:
2042 1662 1149 648 359 159 6
MspA1I CmG'CkG
Cuts at: 1606 2107 2914 3183 3441 4118 5266 5511 1606
Size: 501 807 269 258 677 1148 245 2120
Fragments arranged by size:
2120 1148 807 677 501 269 258 245
NaeI GCC'GGC
Cuts at: 2693 2693
Size: 6025
NcoI C'CATG_G
Cuts at: 513 1065 2004 2197 513
Size: 552 939 193 4341
Fragments arranged by size:
4341 939 552 193
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6025
NgoMIV G'CCGG_C
Cuts at: 2691 2691
Size: 6025
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6025
NotI GC'GGCC_GC
Cuts at: 3472 3472
Size: 6025
NspI r_CATG'y
Cuts at: 1132 1229 2137 3083 3383 4928 1132
Size: 97 908 946 300 1545 2229
Fragments arranged by size:
2229 1545 946 908 300 97
PasI CC'CwG_GG
Cuts at: 1348 3307 1348
Size: 1959 4066
PciI A'CATG_T
Cuts at: 4924 4924
Size: 6025
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6025
PmeI GTTT'AAAC
Cuts at: 3329 3329
Size: 6025
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2348 2380 4094 4126 5607 5639 2348
Size: 32 1714 32 1481 32 2734
Fragments arranged by size:
2734 1714 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2206 2986 3103 2206
Size: 780 117 5128
Fragments arranged by size:
5128 780 117
PshAI GACnn'nnGTC
Cuts at: 1613 3293 1613
Size: 1680 4345
PsiI TTA'TAA
Cuts at: 3595 3595
Size: 6025
PstI C_TGCA'G
Cuts at: 830 2276 2523 2715 3117 3197 3251 3377 830
Size: 1446 247 192 402 80 54 126 3478
Fragments arranged by size:
3478 1446 402 247 192 126 80 54
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6025
PvuII CAG'CTG
Cuts at: 2914 2914
Size: 6025
SalI G'TCGA_C
Cuts at: 1936 3367 1936
Size: 1431 4594
SbfI CC_TGCA'GG
Cuts at: 3377 3377
Size: 6025
ScaI AGT'ACT
Cuts at: 2574 4273 5985 2574
Size: 1699 1712 2614
Fragments arranged by size:
2614 1712 1699
SexAI A'CCwGG_T
Cuts at: 2988 2988
Size: 6025
SfcI C'TryA_G
Cuts at: 826 1045 2272 2519 2711 3113 3139 3193 3247
Size: 219 1227 247 192 402 26 54 54
Cuts at: 3247 3373 4508 5189 5380 826
Size: 126 1135 681 191 1471
Fragments arranged by size:
1471 1227 1135 681 402 247 219 192
191 126 54 54 26
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6025
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6025
SmaI CCC'GGG
Cuts at: 1872 2032 3352 1872
Size: 160 1320 4545
Fragments arranged by size:
4545 1320 160
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2620 2873 4129 5030 5292 820
Size: 197 926 677 253 1256 901 262 1553
Fragments arranged by size:
1553 1256 926 901 677 262 253 197
SnaBI TAC'GTA
Cuts at: 493 3816 493
Size: 3323 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6025
SphI G_CATG'C
Cuts at: 1132 3383 1132
Size: 2251 3774
SspI AAT'ATT
Cuts at: 5 52 3949 5
Size: 47 3897 2081
Fragments arranged by size:
3897 2081 47
StuI AGG'CCT
Cuts at: 1517 2336 1517
Size: 819 5206
StyI C'CwwG_G
Cuts at: 513 1065 2004 2197 2859 3464 513
Size: 552 939 193 662 605 3074
Fragments arranged by size:
3074 939 662 605 552 193
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2210 4052 4069 4222 4407 4852 325
Size: 1255 630 1842 17 153 185 445 1498
Fragments arranged by size:
1842 1498 1255 630 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2572 2802 4271
Size: 274 80 33 51 1443 595 230 1469
Cuts at: 4271 5983 96
Size: 1712 138
Fragments arranged by size:
1712 1469 1443 595 274 230 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2321 2353 5985 6017 2321
Size: 32 3632 32 2329
Fragments arranged by size:
3632 2329 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2402 1316
Size: 1086 4939
XbaI T'CTAG_A
Cuts at: 3361 3361
Size: 6025
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2317 2462 2011
Size: 306 145 5574
Fragments arranged by size:
5574 306 145
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6025
XmaI C'CCGG_G
Cuts at: 1870 2030 3350 1870
Size: 160 1320 4545
Fragments arranged by size:
4545 1320 160
XmnI GAAnn'nnTTC
Cuts at: 2258 2303 4154 2258
Size: 45 1851 4129
Fragments arranged by size:
4129 1851 45
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3833 276
Size: 53 83 186 936 2299 2468
Fragments arranged by size:
2468 2299 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PfoI
PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SfiI SfoI SrfI SwaI