Restriction map for
pFN21AB8333
(Circular) (Six-base) MAPSORT of: pFN21AB8333.seq Check: 2837 from: 1 to: 5644
pFN21AB8333 5644 bp
With 182 enzymes: SgfI *
February 27, 2009 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3454 278
Size: 53 83 186 936 1918 2468
Fragments arranged by size:
2468 1918 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2987 1937
Size: 1050 4594
Acc65I G'GTAC_C
Cuts at: 2419 2965 2419
Size: 546 5098
AclI AA'CG_TT
Cuts at: 1563 3771 4144 1563
Size: 2208 373 3063
Fragments arranged by size:
3063 2208 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2627 3707 5091 5359 1632
Size: 995 1080 1384 268 1917
Fragments arranged by size:
1917 1384 1080 995 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5447
AflIII A'CryG_T
Cuts at: 1203 2854 2856 4543 5520 1203
Size: 1651 2 1687 977 1327
Fragments arranged by size:
1687 1651 1327 977 2
AgeI A'CCGG_T
Cuts at: 4475 4475
Size: 5644
AhdI GACnn_n'nnGTC
Cuts at: 2830 4373 2830
Size: 1543 4101
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5644
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2093 1184
Size: 909 4735
AlwNI CAG_nnn'CTG
Cuts at: 3387 4959 3387
Size: 1572 4072
ApaLI G'TGCA_C
Cuts at: 3701 4857 3701
Size: 1156 4488
ApoI r'AATT_y
Cuts at: 1493 2953 3179 3331 5440 1493
Size: 1460 226 152 2109 1697
Fragments arranged by size:
2109 1697 1460 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4198 160
Size: 872 3166 1606
Fragments arranged by size:
3166 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5644
AvaI C'yCGr_G
Cuts at: 1870 1943 2165 2969 1870
Size: 73 222 804 4545
Fragments arranged by size:
4545 804 222 73
BamHI G'GATC_C
Cuts at: 2641 2974 3341 2641
Size: 333 367 4944
Fragments arranged by size:
4944 367 333
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2045 2096 2419 2550 2965
Size: 325 217 627 258 51 323 131 415
Cuts at: 2965 4420 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 415 325 323 258 217
131 51
BanII G_rGCy'C
Cuts at: 1391 2439 2843 2963 1391
Size: 1048 404 120 4072
Fragments arranged by size:
4072 1048 404 120
BbeI G_GCGC'C
Cuts at: 2100 2554 2100
Size: 454 5190
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4673
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2222 2256 3835 3869 1903
Size: 34 285 34 1579 34 3678
Fragments arranged by size:
3678 1579 285 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3536 4746 3536
Size: 1210 4434
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5644
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4255 136
Size: 107 122 71 631 605 2583 1525
Fragments arranged by size:
2583 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5639 5639
Size: 5644
BlpI GC'TnA_GC
Cuts at: 3061 3061
Size: 5644
Bme1580I G_kGCm'C
Cuts at: 1790 2410 2539 2910 3705 4861 1790
Size: 620 129 371 795 1156 2573
Fragments arranged by size:
2573 1156 795 620 371 129
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5644
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3375 4333 442
Size: 416 53 83 399 24 1958 958 1753
Fragments arranged by size:
1958 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5644
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2035 4304 1239
Size: 796 2269 2579
Fragments arranged by size:
2579 2269 796
Bpu10I CC'TnA_GC
Cuts at: 2148 2741 2148
Size: 593 5051
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3769 4634 4932 3769
Size: 865 298 4481
Fragments arranged by size:
4481 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4307 882
Size: 617 2808 2219
Fragments arranged by size:
2808 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2857 3435 493
Size: 711 1653 578 2702
Fragments arranged by size:
2702 1653 711 578
BsaBI GATnn'nnATC
Cuts at: 5638 5638
Size: 5644
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2097 2257 2551 3451
Size: 53 83 186 936 564 160 294 900
Cuts at: 3451 3833 4468 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 900 635 564 382 294 186
160 83 53
BsaWI w'CCGG_w
Cuts at: 4077 4475 4749 4896 5307 4077
Size: 398 274 147 411 4414
Fragments arranged by size:
4414 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2876 2906 1178
Size: 30 165 30 1473 30 3916
Fragments arranged by size:
3916 1473 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2443 2642 1186
Size: 1257 199 4188
Fragments arranged by size:
4188 1257 199
BseYI C'CCAG_C
Cuts at: 4847 5343 4847
Size: 496 5148
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2612 1163
Size: 573 876 4195
Fragments arranged by size:
4195 876 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2812 3094 3855 4883 1540
Size: 275 186 811 282 761 1028 2301
Fragments arranged by size:
2301 1028 811 761 282 275 186
BsiHKAI G_wGCw'C
Cuts at: 2843 3705 3790 4861 2843
Size: 862 85 1071 3626
Fragments arranged by size:
3626 1071 862 85
BsmI GAATG_Cn'
Cuts at: 2324 3155 3248 2324
Size: 831 93 4720
Fragments arranged by size:
4720 831 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2410 2439 2539 2843 2910 2963 3705
Size: 399 620 29 100 304 67 53 742
Cuts at: 3705 3790 4861 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 620 399 304 100 85
67 53 29
BspEI T'CCGG_A
Cuts at: 5307 5307
Size: 5644
BspHI T'CATG_A
Cuts at: 3533 5263 3533
Size: 1730 3914
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2895 2999 844
Size: 2051 104 3489
Fragments arranged by size:
3489 2051 104
BsrBI CCG'CTC
Cuts at: 1394 1823 3531 1394
Size: 429 1708 3507
Fragments arranged by size:
3507 1708 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2070 4139 4313 65
Size: 1183 822 2069 174 1396
Fragments arranged by size:
2069 1396 1183 822 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4288 4475 1700
Size: 110 2478 187 2869
Fragments arranged by size:
2869 2478 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5644
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5644
BssSI C'ACGA_G
Cuts at: 2540 3704 4716 2540
Size: 1164 1012 3468
Fragments arranged by size:
3468 1164 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5644
BstBI TT'CG_AA
Cuts at: 4448 4448
Size: 5644
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5644
BstYI r'GATC_y
Cuts at: 1972 2641 2728 2974 3341 3726 3743 5195 5281
Size: 669 87 246 367 385 17 1452 86
Cuts at: 5281 5293 5639 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 669 385 367 346 246 87
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2065 2554 513
Size: 552 939 61 489 3603
Fragments arranged by size:
3603 939 552 489 61
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4905
Fragments arranged by size:
4905 683 56
BtsI GCAGTG_nn'
Cuts at: 3160 3954 3974 3160
Size: 794 20 4830
Fragments arranged by size:
4830 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5644
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2372 2407 529
Size: 35 1808 35 3766
Fragments arranged by size:
3766 1808 35 35
DraI TTT'AAA
Cuts at: 2948 2948
Size: 5644
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4651 809
Size: 507 3335 1802
Fragments arranged by size:
3335 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2023 2062 2116
Size: 54 1425 50 275 18 193 39 54
Cuts at: 2116 2428 2579 2809 3091 3980 5609 8
Size: 312 151 230 282 889 1629 43
Fragments arranged by size:
1629 1425 889 312 282 275 230 193
151 54 54 50 43 39 18
EagI C'GGCC_G
Cuts at: 1537 1812 2809 3091 1537
Size: 275 997 282 4090
Fragments arranged by size:
4090 997 282 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3574 1353
Size: 2221 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4231 4605 4751 387
Size: 1064 138 2642 374 146 1280
Fragments arranged by size:
2642 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2841 2841
Size: 5644
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2035 2627 3707 4304 5091 5359 1239
Size: 393 403 592 1080 597 787 268 1524
Fragments arranged by size:
1524 1080 787 597 592 403 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2632 2632
Size: 5644
EcoRI G'AATT_C
Cuts at: 2953 3331 2953
Size: 378 5266
EcoRV GAT'ATC
Cuts at: 2359 2359
Size: 5644
FspI TGC'GCA
Cuts at: 4150 4150
Size: 5644
HaeII r_GCGC'y
Cuts at: 1678 2100 2554 4791 1678
Size: 422 454 2237 2531
Fragments arranged by size:
2531 2237 454 422
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2144 2176 2816 2848 4285
Size: 32 135 32 572 32 640 32 1437
Cuts at: 4285 4317 4359 4391 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1437 640 572 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2275 2988 3234 669
Size: 988 281 337 713 246 3079
Fragments arranged by size:
3079 988 713 337 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5644
HpaI GTT'AAC
Cuts at: 3234 3234
Size: 5644
KasI G'GCGC_C
Cuts at: 2096 2550 2096
Size: 454 5190
KpnI G_GTAC'C
Cuts at: 2423 2969 2423
Size: 546 5098
MfeI C'AATT_G
Cuts at: 3243 3243
Size: 5644
MluI A'CGCG_T
Cuts at: 5520 5520
Size: 5644
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2350 4758 4942 1305
Size: 1045 2408 184 2007
Fragments arranged by size:
2408 2007 1045 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2025 2430 10
Size: 54 1425 343 193 405 3224
Fragments arranged by size:
3224 1425 405 343 193 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3602 3961 4120 518
Size: 648 2436 359 159 2042
Fragments arranged by size:
2436 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2426 2565 3060 3737 4885 5130 1606
Size: 820 139 495 677 1148 245 2120
Fragments arranged by size:
2120 1148 820 677 495 245 139
NarI GG'CG_CC
Cuts at: 2097 2551 2097
Size: 454 5190
NcoI C'CATG_G
Cuts at: 513 1065 2004 2554 513
Size: 552 939 550 3603
Fragments arranged by size:
3603 939 552 550
NdeI CA'TA_TG
Cuts at: 387 2616 387
Size: 2229 3415
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5644
NotI GC'GGCC_GC
Cuts at: 3091 3091
Size: 5644
NspI r_CATG'y
Cuts at: 1132 1229 3002 4547 1132
Size: 97 1773 1545 2229
Fragments arranged by size:
2229 1773 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5644
PciI A'CATG_T
Cuts at: 4543 4543
Size: 5644
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5644
PmeI GTTT'AAAC
Cuts at: 2948 2948
Size: 5644
PmlI CAC'GTG
Cuts at: 2857 2857
Size: 5644
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3713 3745 5226 5258 3713
Size: 32 1481 32 4099
Fragments arranged by size:
4099 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2632 2632
Size: 5644
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5644
PsiI TTA'TAA
Cuts at: 3214 3214
Size: 5644
PstI C_TGCA'G
Cuts at: 830 2472 2640 2996 830
Size: 1642 168 356 3478
Fragments arranged by size:
3478 1642 356 168
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5644
PvuII CAG'CTG
Cuts at: 2426 2565 2426
Size: 139 5505
SacI G_AGCT'C
Cuts at: 2843 2843
Size: 5644
SalI G'TCGA_C
Cuts at: 1936 2986 1936
Size: 1050 4594
SbfI CC_TGCA'GG
Cuts at: 2640 2996 2640
Size: 356 5288
ScaI AGT'ACT
Cuts at: 3892 5604 3892
Size: 1712 3932
SfcI C'TryA_G
Cuts at: 826 1045 2468 2636 2992 4127 4808 4999 826
Size: 219 1423 168 356 1135 681 191 1471
Fragments arranged by size:
1471 1423 1135 681 356 219 191 168
SfoI GGC'GCC
Cuts at: 2098 2552 2098
Size: 454 5190
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5644
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5644
SmaI CCC'GGG
Cuts at: 1872 2971 1872
Size: 1099 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3748 4649 4911 820
Size: 197 926 1805 901 262 1553
Fragments arranged by size:
1805 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3435 493
Size: 2942 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5644
SphI G_CATG'C
Cuts at: 1132 3002 1132
Size: 1870 3774
SspI AAT'ATT
Cuts at: 5 52 3568 5
Size: 47 3516 2081
Fragments arranged by size:
3516 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5644
StyI C'CwwG_G
Cuts at: 513 1065 2004 2554 3083 513
Size: 552 939 550 529 3074
Fragments arranged by size:
3074 939 552 550 529
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3671 3688 3841 4026 4471 325
Size: 1255 2091 17 153 185 445 1498
Fragments arranged by size:
2091 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3890 5602 96
Size: 274 80 33 51 1443 1913 1712 138
Fragments arranged by size:
1913 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5604 5636 5604
Size: 32 5612
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5644
XbaI T'CTAG_A
Cuts at: 2260 2980 2260
Size: 720 4924
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5644
XmaI C'CCGG_G
Cuts at: 1870 2969 1870
Size: 1099 4545
XmnI GAAnn'nnTTC
Cuts at: 2657 3773 2657
Size: 1116 4528
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3452 276
Size: 53 83 186 936 1918 2468
Fragments arranged by size:
2468 1918 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoNI FalI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PfoI PspOMI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SrfI SwaI XcmI