Restriction map for
pFN21AB8332
(Circular) (Six-base) MAPSORT of: pFN21AB8332.seq Check: 196 from: 1 to: 6541
pFN21AB8332 6541 bp
With 182 enzymes: SgfI *
February 27, 2009 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2699 2699
Size: 6541
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4351 278
Size: 53 83 186 936 2815 2468
Fragments arranged by size:
2815 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2364 2393 3399 3884 1937
Size: 427 29 1006 485 4594
Fragments arranged by size:
4594 1006 485 427 29
Acc65I G'GTAC_C
Cuts at: 3604 3862 3604
Size: 258 6283
AclI AA'CG_TT
Cuts at: 1563 4668 5041 1563
Size: 3105 373 3063
Fragments arranged by size:
3105 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2205 2508 2574 3717 4604 5988 6256 1632
Size: 573 303 66 1143 887 1384 268 1917
Fragments arranged by size:
1917 1384 1143 887 573 303 268 66
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6344
AflIII A'CryG_T
Cuts at: 1203 5440 6417 1203
Size: 4237 977 1327
Fragments arranged by size:
4237 1327 977
AgeI A'CCGG_T
Cuts at: 5372 5372
Size: 6541
AhdI GACnn_n'nnGTC
Cuts at: 5270 5270
Size: 6541
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6541
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6541
AlwNI CAG_nnn'CTG
Cuts at: 2056 3059 3224 4284 5856 2056
Size: 1003 165 1060 1572 2741
Fragments arranged by size:
2741 1572 1060 1003 165
ApaLI G'TGCA_C
Cuts at: 4598 5754 4598
Size: 1156 5385
ApoI r'AATT_y
Cuts at: 1493 2588 2951 3144 3850 4076 4228 6337 1493
Size: 1095 363 193 706 226 152 2109 1697
Fragments arranged by size:
2109 1697 1095 706 363 226 193 152
AseI AT'TA_AT
Cuts at: 160 1032 3789 5095 160
Size: 872 2757 1306 1606
Fragments arranged by size:
2757 1606 1306 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6541
AvaI C'yCGr_G
Cuts at: 1870 1943 2376 3866 1870
Size: 73 433 1490 4545
Fragments arranged by size:
4545 1490 433 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3588 3621 3588
Size: 33 6508
BamHI G'GATC_C
Cuts at: 3871 4238 3871
Size: 367 6174
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2306 3604 3862 5317 618
Size: 325 217 627 519 1298 258 1455 1842
Fragments arranged by size:
1842 1455 1298 627 519 325 258 217
BanII G_rGCy'C
Cuts at: 1391 2635 3860 1391
Size: 1244 1225 4072
Fragments arranged by size:
4072 1244 1225
BbeI G_GCGC'C
Cuts at: 2310 2310
Size: 6541
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2691 928
Size: 971 792 4778
Fragments arranged by size:
4778 971 792
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4732 4766 1903
Size: 34 2795 34 3678
Fragments arranged by size:
3678 2795 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2808 4433 5643 2808
Size: 1625 1210 3706
Fragments arranged by size:
3706 1625 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6541
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3312 5152 136
Size: 107 122 71 631 605 1640 1840 1525
Fragments arranged by size:
1840 1640 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2158 6536 2158
Size: 4378 2163
BlpI GC'TnA_GC
Cuts at: 3958 3958
Size: 6541
Bme1580I G_kGCm'C
Cuts at: 1790 3763 4602 5758 1790
Size: 1973 839 1156 2573
Fragments arranged by size:
2573 1973 1156 839
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6541
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4272 5230 442
Size: 416 53 83 399 24 2855 958 1753
Fragments arranged by size:
2855 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6541
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2199 2319 2397 3015 5201 1239
Size: 960 120 78 618 2186 2579
Fragments arranged by size:
2579 2186 960 618 120 78
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4666 5531 5829 4666
Size: 865 298 5378
Fragments arranged by size:
5378 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5204 882
Size: 617 3705 2219
Fragments arranged by size:
3705 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2391 4332 493
Size: 711 1187 1941 2702
Fragments arranged by size:
2702 1941 1187 711
BsaBI GATnn'nnATC
Cuts at: 2651 3125 6535 2651
Size: 474 3410 2657
Fragments arranged by size:
3410 2657 474
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2307 4348 4730 5365
Size: 53 83 186 936 774 2041 382 635
Cuts at: 5365 275
Size: 1451
Fragments arranged by size:
2041 1451 936 774 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2800 4974 5372 5646 5793 6204 2800
Size: 2174 398 274 147 411 3137
Fragments arranged by size:
3137 2174 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2395 2425 1178
Size: 30 165 30 992 30 5294
Fragments arranged by size:
5294 992 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2226 2364 3827 1186
Size: 1040 138 1463 3900
Fragments arranged by size:
3900 1463 1040 138
BseYI C'CCAG_C
Cuts at: 3535 5744 6240 3535
Size: 2209 496 3836
Fragments arranged by size:
3836 2209 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3045 1163
Size: 573 1309 4659
Fragments arranged by size:
4659 1309 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3991 4752 5780 1540
Size: 275 186 1990 761 1028 2301
Fragments arranged by size:
2301 1990 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2270 2635 4602 4687 5758 2270
Size: 365 1967 85 1071 3053
Fragments arranged by size:
3053 1967 1071 365 85
BsmI GAATG_Cn'
Cuts at: 4052 4145 4052
Size: 93 6448
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2270 2635 3763 3860 4602 4687 5758
Size: 399 480 365 1128 97 742 85 1071
Cuts at: 5758 1391
Size: 2174
Fragments arranged by size:
2174 1128 1071 742 480 399 365 97
85
BspEI T'CCGG_A
Cuts at: 2800 6204 2800
Size: 3404 3137
BspHI T'CATG_A
Cuts at: 2542 2845 4430 6160 2542
Size: 303 1585 1730 2923
Fragments arranged by size:
2923 1730 1585 303
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2699 3844 3896 844
Size: 1855 1145 52 3489
Fragments arranged by size:
3489 1855 1145 52
BsrBI CCG'CTC
Cuts at: 1394 1823 4428 1394
Size: 429 2605 3507
Fragments arranged by size:
3507 2605 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2189 3754 5036 5210 65
Size: 1183 941 1565 1282 174 1396
Fragments arranged by size:
1565 1396 1282 1183 941 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5185 5372 1700
Size: 110 3375 187 2869
Fragments arranged by size:
3375 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3294 96
Size: 3198 3343
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6541
BssSI C'ACGA_G
Cuts at: 2810 4601 5613 2810
Size: 1791 1012 3738
Fragments arranged by size:
3738 1791 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6541
BstBI TT'CG_AA
Cuts at: 5345 5345
Size: 6541
BstEII G'GTnAC_C
Cuts at: 1184 3522 1184
Size: 2338 4203
BstXI CCAn_nnnn'nTGG
Cuts at: 2297 2297
Size: 6541
BstYI r'GATC_y
Cuts at: 1972 2158 2315 3871 4238 4623 4640 6092 6178
Size: 186 157 1556 367 385 17 1452 86
Cuts at: 6178 6190 6536 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1556 1452 385 367 346 186 157
86 17 12
BstZ17I GTA'TAC
Cuts at: 2394 2394
Size: 6541
BtgI C'CryG_G
Cuts at: 513 1065 2310 3310 513
Size: 552 1245 1000 3744
Fragments arranged by size:
3744 1245 1000 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5802
Fragments arranged by size:
5802 683 56
BtsI GCAGTG_nn'
Cuts at: 2914 4057 4851 4871 2914
Size: 1143 794 20 4584
Fragments arranged by size:
4584 1143 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6541
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6506
DraI TTT'AAA
Cuts at: 2505 3845 2505
Size: 1340 5201
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3235 5548 809
Size: 507 1919 2313 1802
Fragments arranged by size:
2313 1919 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3769 3988 4877
Size: 54 1425 50 275 18 1939 219 889
Cuts at: 4877 6506 8
Size: 1629 43
Fragments arranged by size:
1939 1629 1425 889 275 219 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3988 1537
Size: 275 2176 4090
Fragments arranged by size:
4090 2176 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2075 2436 2487 4471 1353
Size: 722 361 51 1984 3423
Fragments arranged by size:
3423 1984 722 361 51
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5128 5502 5648 387
Size: 1064 138 3539 374 146 1280
Fragments arranged by size:
3539 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2633 2633
Size: 6541
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2199 2205 2319 2397 2508 2574 3015
Size: 393 567 6 114 78 111 66 441
Cuts at: 3015 3717 4604 5201 5988 6256 1239
Size: 702 887 597 787 268 1524
Fragments arranged by size:
1524 887 787 702 597 567 441 393
268 114 111 78 66 6
EcoO109I rG'GnC_Cy
Cuts at: 2327 2327
Size: 6541
EcoRI G'AATT_C
Cuts at: 3850 4228 3850
Size: 378 6163
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2210 2210
Size: 6541
FspI TGC'GCA
Cuts at: 5047 5047
Size: 6541
HaeII r_GCGC'y
Cuts at: 1678 2310 5688 1678
Size: 632 3378 2531
Fragments arranged by size:
3378 2531 632
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2370 2402 2738 2770 2774
Size: 32 135 32 798 32 336 32 4
Cuts at: 2774 2806 5182 5214 5256 5288 1373
Size: 32 2376 32 42 32 2626
Fragments arranged by size:
2626 2376 798 336 135 42 32 32
32 32 32 32 32 4
HincII GTy'rAC
Cuts at: 669 1657 1938 2365 3259 3885 4131 669
Size: 988 281 427 894 626 246 3079
Fragments arranged by size:
3079 988 894 626 427 281 246
HindIII A'AGCT_T
Cuts at: 748 3446 748
Size: 2698 3843
HpaI GTT'AAC
Cuts at: 4131 4131
Size: 6541
KasI G'GCGC_C
Cuts at: 2306 2306
Size: 6541
KpnI G_GTAC'C
Cuts at: 3608 3866 3608
Size: 258 6283
MfeI C'AATT_G
Cuts at: 4140 4140
Size: 6541
MluI A'CGCG_T
Cuts at: 6417 6417
Size: 6541
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2016 3076 3229 3370 5655 5839 1305
Size: 711 1060 153 141 2285 184 2007
Fragments arranged by size:
2285 2007 1060 711 184 153 141
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4719
Fragments arranged by size:
4719 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3378 3623 4499 4858 5017 518
Size: 648 2212 245 876 359 159 2042
Fragments arranged by size:
2212 2042 876 648 359 245 159
MspA1I CmG'CkG
Cuts at: 1606 2927 3059 3386 3957 4634 5782 6027 1606
Size: 1321 132 327 571 677 1148 245 2120
Fragments arranged by size:
2120 1321 1148 677 571 327 245 132
NarI GG'CG_CC
Cuts at: 2307 2307
Size: 6541
NcoI C'CATG_G
Cuts at: 513 1065 3310 513
Size: 552 2245 3744
Fragments arranged by size:
3744 2245 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6541
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6541
NotI GC'GGCC_GC
Cuts at: 3988 3988
Size: 6541
NspI r_CATG'y
Cuts at: 1132 1229 3169 3306 3899 5444 1132
Size: 97 1940 137 593 1545 2229
Fragments arranged by size:
2229 1940 1545 593 137 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6541
PciI A'CATG_T
Cuts at: 5440 5440
Size: 6541
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3365 1108
Size: 2257 4284
PmeI GTTT'AAAC
Cuts at: 3845 3845
Size: 6541
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4610 4642 6123 6155 4610
Size: 32 1481 32 4996
Fragments arranged by size:
4996 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2327 2327
Size: 6541
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6541
PsiI TTA'TAA
Cuts at: 4111 4111
Size: 6541
PstI C_TGCA'G
Cuts at: 830 3893 830
Size: 3063 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6541
PvuII CAG'CTG
Cuts at: 2927 3059 3386 2927
Size: 132 327 6082
Fragments arranged by size:
6082 327 132
SacI G_AGCT'C
Cuts at: 2635 2635
Size: 6541
SalI G'TCGA_C
Cuts at: 1936 2363 3883 1936
Size: 427 1520 4594
Fragments arranged by size:
4594 1520 427
SbfI CC_TGCA'GG
Cuts at: 3893 3893
Size: 6541
ScaI AGT'ACT
Cuts at: 4789 6501 4789
Size: 1712 4829
SfcI C'TryA_G
Cuts at: 826 1045 2665 3889 5024 5705 5896 826
Size: 219 1620 1224 1135 681 191 1471
Fragments arranged by size:
1620 1471 1224 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2308 2308
Size: 6541
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6541
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6541
SmaI CCC'GGG
Cuts at: 1872 3868 1872
Size: 1996 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2376 4645 5546 5808 820
Size: 197 926 433 2269 901 262 1553
Fragments arranged by size:
2269 1553 926 901 433 262 197
SnaBI TAC'GTA
Cuts at: 493 4332 493
Size: 3839 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6541
SphI G_CATG'C
Cuts at: 1132 3306 3899 1132
Size: 2174 593 3774
Fragments arranged by size:
3774 2174 593
SspI AAT'ATT
Cuts at: 5 52 4465 5
Size: 47 4413 2081
Fragments arranged by size:
4413 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6541
StyI C'CwwG_G
Cuts at: 513 1065 3310 3980 513
Size: 552 2245 670 3074
Fragments arranged by size:
3074 2245 670 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3549 3677 4568 4585 4738 4923 5368
Size: 1255 1969 128 891 17 153 185 445
Cuts at: 5368 325
Size: 1498
Fragments arranged by size:
1969 1498 1255 891 445 185 153 128
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3294 4787 6499
Size: 274 80 33 51 1443 1317 1493 1712
Cuts at: 6499 96
Size: 138
Fragments arranged by size:
1712 1493 1443 1317 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6501 6533 6501
Size: 32 6509
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6541
XbaI T'CTAG_A
Cuts at: 2318 3877 2318
Size: 1559 4982
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3622 3622
Size: 6541
XhoI C'TCGA_G
Cuts at: 1943 2376 1943
Size: 433 6108
XmaI C'CCGG_G
Cuts at: 1870 3866 1870
Size: 1996 4545
XmnI GAAnn'nnTTC
Cuts at: 4670 4670
Size: 6541
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4349 276
Size: 53 83 186 936 2815 2468
Fragments arranged by size:
2815 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI Bsu36I DraIII EcoNI EcoRV FseI
FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI
PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI
SfiI SrfI SwaI