Restriction map for
pFN21AB8330
(Circular) (Six-base) MAPSORT of: pFN21AB8330.seq Check: 1433 from: 1 to: 5116
pFN21AB8330 5116 bp
With 182 enzymes: SgfI *
August 16, 2010 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2926 278
Size: 53 83 186 936 1390 2468
Fragments arranged by size:
2468 1390 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2459 1937
Size: 522 4594
Acc65I G'GTAC_C
Cuts at: 2437 2437
Size: 5116
AclI AA'CG_TT
Cuts at: 1563 3243 3616 1563
Size: 1680 373 3063
Fragments arranged by size:
3063 1680 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3179 4563 4831 1632
Size: 1547 1384 268 1917
Fragments arranged by size:
1917 1547 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4919
AflIII A'CryG_T
Cuts at: 1203 4015 4992 1203
Size: 2812 977 1327
Fragments arranged by size:
2812 1327 977
AgeI A'CCGG_T
Cuts at: 3947 3947
Size: 5116
AhdI GACnn_n'nnGTC
Cuts at: 3845 3845
Size: 5116
AleI CACnn'nnGTG
Cuts at: 1166 2198 1166
Size: 1032 4084
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5116
AlwNI CAG_nnn'CTG
Cuts at: 2859 4431 2859
Size: 1572 3544
ApaI G_GGCC'C
Cuts at: 2235 2235
Size: 5116
ApaLI G'TGCA_C
Cuts at: 3173 4329 3173
Size: 1156 3960
ApoI r'AATT_y
Cuts at: 1493 2240 2425 2651 2803 4912 1493
Size: 747 185 226 152 2109 1697
Fragments arranged by size:
2109 1697 747 226 185 152
AseI AT'TA_AT
Cuts at: 160 1032 3670 160
Size: 872 2638 1606
Fragments arranged by size:
2638 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5116
AvaI C'yCGr_G
Cuts at: 1870 1943 2441 1870
Size: 73 498 4545
Fragments arranged by size:
4545 498 73
BamHI G'GATC_C
Cuts at: 2446 2813 2446
Size: 367 4749
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2034 2437 3892 618
Size: 325 217 627 247 403 1455 1842
Fragments arranged by size:
1842 1455 627 403 325 247 217
BanII G_rGCy'C
Cuts at: 1391 2235 2435 1391
Size: 844 200 4072
Fragments arranged by size:
4072 844 200
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4145
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3307 3341 1903
Size: 34 1370 34 3678
Fragments arranged by size:
3678 1370 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3008 4218 3008
Size: 1210 3906
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5116
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3727 136
Size: 107 122 71 631 605 2055 1525
Fragments arranged by size:
2055 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5111 5111
Size: 5116
BlpI GC'TnA_GC
Cuts at: 2533 2533
Size: 5116
Bme1580I G_kGCm'C
Cuts at: 1790 2235 3177 4333 1790
Size: 445 942 1156 2573
Fragments arranged by size:
2573 1156 942 445
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5116
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2847 3805 442
Size: 416 53 83 399 24 1430 958 1753
Fragments arranged by size:
1753 1430 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5116
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2402 3776 1239
Size: 1163 1374 2579
Fragments arranged by size:
2579 1374 1163
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3241 4106 4404 3241
Size: 865 298 3953
Fragments arranged by size:
3953 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2072 3779 882
Size: 617 573 1707 2219
Fragments arranged by size:
2219 1707 617 573
BsaAI yAC'GTr
Cuts at: 493 1204 2907 493
Size: 711 1703 2702
Fragments arranged by size:
2702 1703 711
BsaBI GATnn'nnATC
Cuts at: 5110 5110
Size: 5116
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2923 3305 3940 275
Size: 53 83 186 936 1390 382 635 1451
Fragments arranged by size:
1451 1390 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2184 3549 3947 4221 4368 4779 2184
Size: 1365 398 274 147 411 2521
Fragments arranged by size:
2521 1365 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2110 2140 1178
Size: 30 165 30 707 30 4154
Fragments arranged by size:
4154 707 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5116
BseYI C'CCAG_C
Cuts at: 2053 4319 4815 2053
Size: 2266 496 2354
Fragments arranged by size:
2354 2266 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2073 2372 1163
Size: 573 337 299 3907
Fragments arranged by size:
3907 573 337 299
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2566 3327 4355 1540
Size: 275 186 565 761 1028 2301
Fragments arranged by size:
2301 1028 761 565 275 186
BsiHKAI G_wGCw'C
Cuts at: 3177 3262 4333 3177
Size: 85 1071 3960
Fragments arranged by size:
3960 1071 85
BsmI GAATG_Cn'
Cuts at: 2627 2720 2627
Size: 93 5023
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2235 2435 3177 3262 4333 1391
Size: 399 445 200 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 445 399 200 85
BspEI T'CCGG_A
Cuts at: 4779 4779
Size: 5116
BspHI T'CATG_A
Cuts at: 3005 4735 3005
Size: 1730 3386
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2471 844
Size: 1627 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3003 1394
Size: 429 1180 3507
Fragments arranged by size:
3507 1180 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3611 3785 65
Size: 1183 2363 174 1396
Fragments arranged by size:
2363 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3760 3947 1700
Size: 110 1950 187 2869
Fragments arranged by size:
2869 1950 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5116
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5116
BssSI C'ACGA_G
Cuts at: 2390 3176 4188 2390
Size: 786 1012 3318
Fragments arranged by size:
3318 1012 786
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5116
BstBI TT'CG_AA
Cuts at: 3920 3920
Size: 5116
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5116
BstXI CCAn_nnnn'nTGG
Cuts at: 2161 2200 2161
Size: 39 5077
BstYI r'GATC_y
Cuts at: 1972 2446 2813 3198 3215 4667 4753 4765 5111
Size: 474 367 385 17 1452 86 12 346
Cuts at: 5111 1972
Size: 1977
Fragments arranged by size:
1977 1452 474 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2131 513
Size: 552 1066 3498
Fragments arranged by size:
3498 1066 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4377
Fragments arranged by size:
4377 683 56
BtsI GCAGTG_nn'
Cuts at: 2632 3426 3446 2632
Size: 794 20 4302
Fragments arranged by size:
4302 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5116
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5081
DraI TTT'AAA
Cuts at: 2420 2420
Size: 5116
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2348 4123 809
Size: 507 1032 1775 1802
Fragments arranged by size:
1802 1775 1032 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2202 2563 3452
Size: 54 1425 50 275 18 372 361 889
Cuts at: 3452 5081 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 372 361 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2563 1537
Size: 275 751 4090
Fragments arranged by size:
4090 751 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3046 1353
Size: 1693 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3703 4077 4223 387
Size: 1064 138 2114 374 146 1280
Fragments arranged by size:
2114 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2402 3179 3776 4563 4831 1239
Size: 393 770 777 597 787 268 1524
Fragments arranged by size:
1524 787 777 770 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2210 2231 2232 2210
Size: 21 1 5094
Fragments arranged by size:
5094 21 1
EcoRI G'AATT_C
Cuts at: 2425 2803 2425
Size: 378 4738
FspI TGC'GCA
Cuts at: 3622 3622
Size: 5116
HaeII r_GCGC'y
Cuts at: 1678 4263 1678
Size: 2585 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2171 2203 3757 3789 3831
Size: 32 135 32 599 32 1554 32 42
Cuts at: 3831 3863 1373
Size: 32 2626
Fragments arranged by size:
2626 1554 599 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2460 2706 669
Size: 988 281 522 246 3079
Fragments arranged by size:
3079 988 522 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5116
HpaI GTT'AAC
Cuts at: 2706 2706
Size: 5116
KpnI G_GTAC'C
Cuts at: 2441 2441
Size: 5116
MfeI C'AATT_G
Cuts at: 2715 2715
Size: 5116
MluI A'CGCG_T
Cuts at: 4992 4992
Size: 5116
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4230 4414 1305
Size: 2925 184 2007
Fragments arranged by size:
2925 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3294
Fragments arranged by size:
3294 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2198 3074 3433 3592 518
Size: 648 1032 876 359 159 2042
Fragments arranged by size:
2042 1032 876 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2133 2532 3209 4357 4602 1606
Size: 527 399 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 527 399 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4564
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5116
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5116
NotI GC'GGCC_GC
Cuts at: 2563 2563
Size: 5116
NspI r_CATG'y
Cuts at: 1132 1229 2474 4019 1132
Size: 97 1245 1545 2229
Fragments arranged by size:
2229 1545 1245 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5116
PciI A'CATG_T
Cuts at: 4015 4015
Size: 5116
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5116
PfoI T'CCnGG_A
Cuts at: 2114 2114
Size: 5116
PmeI GTTT'AAAC
Cuts at: 2420 2420
Size: 5116
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3185 3217 4698 4730 3185
Size: 32 1481 32 3571
Fragments arranged by size:
3571 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2210 2210
Size: 5116
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5116
PsiI TTA'TAA
Cuts at: 2686 2686
Size: 5116
PspOMI G'GGCC_C
Cuts at: 2231 2231
Size: 5116
PstI C_TGCA'G
Cuts at: 830 2468 830
Size: 1638 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5116
SacII CC_GC'GG
Cuts at: 2134 2134
Size: 5116
SalI G'TCGA_C
Cuts at: 1936 2458 1936
Size: 522 4594
SbfI CC_TGCA'GG
Cuts at: 2468 2468
Size: 5116
ScaI AGT'ACT
Cuts at: 3364 5076 3364
Size: 1712 3404
SfcI C'TryA_G
Cuts at: 826 1045 2464 3599 4280 4471 826
Size: 219 1419 1135 681 191 1471
Fragments arranged by size:
1471 1419 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5116
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5116
SmaI CCC'GGG
Cuts at: 1872 2443 1872
Size: 571 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3220 4121 4383 820
Size: 197 926 1277 901 262 1553
Fragments arranged by size:
1553 1277 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2907 493
Size: 2414 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5116
SphI G_CATG'C
Cuts at: 1132 2474 1132
Size: 1342 3774
SspI AAT'ATT
Cuts at: 5 52 3040 5
Size: 47 2988 2081
Fragments arranged by size:
2988 2081 47
StuI AGG'CCT
Cuts at: 1517 2331 2369 1517
Size: 814 38 4264
Fragments arranged by size:
4264 814 38
StyI C'CwwG_G
Cuts at: 513 1065 2122 2172 2360 2555 513
Size: 552 1057 50 188 195 3074
Fragments arranged by size:
3074 1057 552 195 188 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3143 3160 3313 3498 3943 325
Size: 1255 1563 17 153 185 445 1498
Fragments arranged by size:
1563 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3362 5074 96
Size: 274 80 33 51 1443 1385 1712 138
Fragments arranged by size:
1712 1443 1385 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5076 5108 5076
Size: 32 5084
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5116
XbaI T'CTAG_A
Cuts at: 2452 2452
Size: 5116
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5116
XmaI C'CCGG_G
Cuts at: 1870 2441 1870
Size: 571 4545
XmnI GAAnn'nnTTC
Cuts at: 3245 3245
Size: 5116
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2924 276
Size: 53 83 186 936 1390 2468
Fragments arranged by size:
2468 1390 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI SacII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI
PvuII RsrII SacI SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI