Restriction map for
pFN21AB8326
(Circular) (Six-base) MAPSORT of: pFN21AB8326.seq Check: 5137 from: 1 to: 5803
pFN21AB8326 5803 bp
With 182 enzymes: SgfI *
February 27, 2009 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2248 2592 2248
Size: 344 5459
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3613 278
Size: 53 83 186 936 2077 2468
Fragments arranged by size:
2468 2077 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3146 1937
Size: 1209 4594
Acc65I G'GTAC_C
Cuts at: 3124 3124
Size: 5803
AclI AA'CG_TT
Cuts at: 1563 3930 4303 1563
Size: 2367 373 3063
Fragments arranged by size:
3063 2367 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3866 5250 5518 1632
Size: 2234 1384 268 1917
Fragments arranged by size:
2234 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5606
AflIII A'CryG_T
Cuts at: 1203 4702 5679 1203
Size: 3499 977 1327
Fragments arranged by size:
3499 1327 977
AgeI A'CCGG_T
Cuts at: 4634 4634
Size: 5803
AhdI GACnn_n'nnGTC
Cuts at: 4532 4532
Size: 5803
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5803
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5803
AlwNI CAG_nnn'CTG
Cuts at: 2023 2176 2356 3546 5118 2023
Size: 153 180 1190 1572 2708
Fragments arranged by size:
2708 1572 1190 180 153
ApaI G_GGCC'C
Cuts at: 2674 2674
Size: 5803
ApaLI G'TGCA_C
Cuts at: 2579 3860 5016 2579
Size: 1281 1156 3366
Fragments arranged by size:
3366 1281 1156
ApoI r'AATT_y
Cuts at: 1493 3112 3338 3490 5599 1493
Size: 1619 226 152 2109 1697
Fragments arranged by size:
2109 1697 1619 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4357 160
Size: 872 3325 1606
Fragments arranged by size:
3325 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5803
AvaI C'yCGr_G
Cuts at: 1870 1943 2570 3128 1870
Size: 73 627 558 4545
Fragments arranged by size:
4545 627 558 73
BamHI G'GATC_C
Cuts at: 3133 3500 3133
Size: 367 5436
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2333 2495 2593 2917 3124
Size: 325 217 627 546 162 98 324 207
Cuts at: 3124 4579 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 546 325 324 217 207
162 98
BanII G_rGCy'C
Cuts at: 1391 2674 2910 2985 3096 3122 1391
Size: 1283 236 75 111 26 4072
Fragments arranged by size:
4072 1283 236 111 75 26
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2622 928
Size: 971 723 4109
Fragments arranged by size:
4109 971 723
BbvCI CC'TCA_GC
Cuts at: 2471 2471
Size: 5803
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3994 4028 1903
Size: 34 2057 34 3678
Fragments arranged by size:
3678 2057 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3695 4905 3695
Size: 1210 4593
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5803
BfrBI ATG'CAT
Cuts at: 2302 2302
Size: 5803
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2186 2686 4414
Size: 107 122 71 631 605 514 500 1728
Cuts at: 4414 136
Size: 1525
Fragments arranged by size:
1728 1525 631 605 514 500 122 107
71
BglII A'GATC_T
Cuts at: 5798 5798
Size: 5803
BlpI GC'TnA_GC
Cuts at: 3220 3220
Size: 5803
Bme1580I G_kGCm'C
Cuts at: 1790 2080 2217 2583 2598 2674 3002 3864 5020
Size: 290 137 366 15 76 328 862 1156
Cuts at: 5020 1790
Size: 2573
Fragments arranged by size:
2573 1156 862 366 328 290 137 76
15
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5803
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3534 4492 442
Size: 416 53 83 399 24 2117 958 1753
Fragments arranged by size:
2117 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5803
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2165 2685 2801 4463 1239
Size: 926 520 116 1662 2579
Fragments arranged by size:
2579 1662 926 520 116
Bpu10I CC'TnA_GC
Cuts at: 2338 2471 2338
Size: 133 5670
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3928 4793 5091 3928
Size: 865 298 4640
Fragments arranged by size:
4640 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2144 2413 2548 4466 882
Size: 617 645 269 135 1918 2219
Fragments arranged by size:
2219 1918 645 617 269 135
BsaAI yAC'GTr
Cuts at: 493 1204 3594 493
Size: 711 2390 2702
Fragments arranged by size:
2702 2390 711
BsaBI GATnn'nnATC
Cuts at: 5797 5797
Size: 5803
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3048 3610 3992 4627
Size: 53 83 186 936 1515 562 382 635
Cuts at: 4627 275
Size: 1451
Fragments arranged by size:
1515 1451 936 635 562 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2803 4236 4634 4908 5055 5466 2803
Size: 1433 398 274 147 411 3140
Fragments arranged by size:
3140 1433 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2774 2804 1178
Size: 30 165 30 1371 30 4177
Fragments arranged by size:
4177 1371 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2162 2611 2727 2798 2829 2832 1186
Size: 976 449 116 71 31 3 4157
Fragments arranged by size:
4157 976 449 116 71 31 3
BseYI C'CCAG_C
Cuts at: 2281 2717 2909 2913 3095 5006 5502 2281
Size: 436 192 4 182 1911 496 2582
Fragments arranged by size:
2582 1911 496 436 192 182 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2138 1163
Size: 573 402 4828
Fragments arranged by size:
4828 573 402
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2646 3253 4014 5042 1540
Size: 275 186 645 607 761 1028 2301
Fragments arranged by size:
2301 1028 761 645 607 275 186
BsiHKAI G_wGCw'C
Cuts at: 2583 2985 3864 3949 5020 2583
Size: 402 879 85 1071 3366
Fragments arranged by size:
3366 1071 879 402 85
BsmI GAATG_Cn'
Cuts at: 3314 3407 3314
Size: 93 5710
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2080 2217 2583 2598 2674 2910 2985
Size: 399 290 137 366 15 76 236 75
Cuts at: 2985 3002 3096 3122 3864 3949 5020 1391
Size: 17 94 26 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 366 290 236 137
94 85 76 75 26 17 15
BspEI T'CCGG_A
Cuts at: 5466 5466
Size: 5803
BspHI T'CATG_A
Cuts at: 3692 5422 3692
Size: 1730 4073
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2244 2248 2592 2841 3158 844
Size: 1400 4 344 249 317 3489
Fragments arranged by size:
3489 1400 344 317 249 4
BsrBI CCG'CTC
Cuts at: 1394 1823 3690 1394
Size: 429 1867 3507
Fragments arranged by size:
3507 1867 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4298 4472 65
Size: 1183 3050 174 1396
Fragments arranged by size:
3050 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2587 2645 4447 4634 1700
Size: 110 777 58 1802 187 2869
Fragments arranged by size:
2869 1802 777 187 110 58
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5803
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5803
BssSI C'ACGA_G
Cuts at: 3863 4875 3863
Size: 1012 4791
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2221 794
Size: 1427 4376
BstBI TT'CG_AA
Cuts at: 4607 4607
Size: 5803
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5803
BstXI CCAn_nnnn'nTGG
Cuts at: 2725 2725
Size: 5803
BstYI r'GATC_y
Cuts at: 1972 2037 3133 3500 3885 3902 5354 5440 5452
Size: 65 1096 367 385 17 1452 86 12
Cuts at: 5452 5798 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1096 385 367 346 86 65
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2154 2455 513
Size: 552 939 150 301 3861
Fragments arranged by size:
3861 939 552 301 150
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2362 487
Size: 683 56 1136 3928
Fragments arranged by size:
3928 1136 683 56
BtsI GCAGTG_nn'
Cuts at: 3319 4113 4133 3319
Size: 794 20 4989
Fragments arranged by size:
4989 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5803
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5768
DraI TTT'AAA
Cuts at: 3107 3107
Size: 5803
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4810 809
Size: 507 3494 1802
Fragments arranged by size:
3494 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2047 2178 2208
Size: 54 1425 50 275 18 217 131 30
Cuts at: 2208 2365 2452 2643 3250 4139 5768 8
Size: 157 87 191 607 889 1629 43
Fragments arranged by size:
1629 1425 889 607 275 217 191 157
131 87 54 50 43 30 18
EagI C'GGCC_G
Cuts at: 1537 1812 2643 3250 1537
Size: 275 831 607 4090
Fragments arranged by size:
4090 831 607 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2805 3733 1353
Size: 1452 928 3423
Fragments arranged by size:
3423 1452 928
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 2530 2602 4390 4764 4910
Size: 1064 138 435 506 72 1788 374 146
Cuts at: 4910 387
Size: 1280
Fragments arranged by size:
1788 1280 1064 506 435 374 146 138
72
EcoICRI GAG'CTC
Cuts at: 2983 2983
Size: 5803
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2165 2685 2801 3866 4463 5250 5518
Size: 393 533 520 116 1065 597 787 268
Cuts at: 5518 1239
Size: 1524
Fragments arranged by size:
1524 1065 787 597 533 520 393 268
116
EcoNI CCTnn'n_nnAGG
Cuts at: 2255 2413 2255
Size: 158 5645
EcoO109I rG'GnC_Cy
Cuts at: 2467 2521 2670 2671 2467
Size: 54 149 1 5599
Fragments arranged by size:
5599 149 54 1
EcoRI G'AATT_C
Cuts at: 3112 3490 3112
Size: 378 5425
FspI TGC'GCA
Cuts at: 2225 4309 2225
Size: 2084 3719
HaeII r_GCGC'y
Cuts at: 1678 2144 2655 4950 1678
Size: 466 511 2295 2531
Fragments arranged by size:
2531 2295 511 466
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2774 2806 4444 4476 4518
Size: 32 135 32 1202 32 1638 32 42
Cuts at: 4518 4550 1373
Size: 32 2626
Fragments arranged by size:
2626 1638 1202 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3147 3393 669
Size: 988 281 1209 246 3079
Fragments arranged by size:
3079 1209 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5803
HpaI GTT'AAC
Cuts at: 3393 3393
Size: 5803
KpnI G_GTAC'C
Cuts at: 3128 3128
Size: 5803
MfeI C'AATT_G
Cuts at: 3402 3402
Size: 5803
MluI A'CGCG_T
Cuts at: 5679 5679
Size: 5803
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2211 4917 5101 1305
Size: 906 2706 184 2007
Fragments arranged by size:
2706 2007 906 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2180 2454 10
Size: 54 1425 343 348 274 3359
Fragments arranged by size:
3359 1425 348 343 274 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2303 3761 4120 4279 518
Size: 648 1137 1458 359 159 2042
Fragments arranged by size:
2042 1458 1137 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2023 2353 2482 2767 2913 3099 3219 3896
Size: 417 330 129 285 146 186 120 677
Cuts at: 3896 5044 5289 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 417 330 285 245 186
146 129 120
NaeI GCC'GGC
Cuts at: 2647 2647
Size: 5803
NcoI C'CATG_G
Cuts at: 513 1065 2004 2154 2455 513
Size: 552 939 150 301 3861
Fragments arranged by size:
3861 939 552 301 150
NdeI CA'TA_TG
Cuts at: 387 2304 387
Size: 1917 3886
NgoMIV G'CCGG_C
Cuts at: 2645 2645
Size: 5803
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5803
NotI GC'GGCC_GC
Cuts at: 3250 3250
Size: 5803
NsiI A_TGCA'T
Cuts at: 2304 2304
Size: 5803
NspI r_CATG'y
Cuts at: 1132 1229 2230 2302 3161 4706 1132
Size: 97 1001 72 859 1545 2229
Fragments arranged by size:
2229 1545 1001 859 97 72
PasI CC'CwG_GG
Cuts at: 1348 3077 1348
Size: 1729 4074
PciI A'CATG_T
Cuts at: 4702 4702
Size: 5803
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2176 2894 1108
Size: 1068 718 4017
Fragments arranged by size:
4017 1068 718
PmeI GTTT'AAAC
Cuts at: 3107 3107
Size: 5803
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3872 3904 5385 5417 3872
Size: 32 1481 32 4258
Fragments arranged by size:
4258 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2467 2467
Size: 5803
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5803
PsiI TTA'TAA
Cuts at: 3373 3373
Size: 5803
PspOMI G'GGCC_C
Cuts at: 2670 2670
Size: 5803
PstI C_TGCA'G
Cuts at: 830 2028 2241 2417 3155 830
Size: 1198 213 176 738 3478
Fragments arranged by size:
3478 1198 738 213 176
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5803
PvuII CAG'CTG
Cuts at: 2023 2482 2913 3099 2023
Size: 459 431 186 4727
Fragments arranged by size:
4727 459 431 186
SacI G_AGCT'C
Cuts at: 2985 2985
Size: 5803
SalI G'TCGA_C
Cuts at: 1936 3145 1936
Size: 1209 4594
SapI GCTCTTCn'nnn_
Cuts at: 2805 2805
Size: 5803
SbfI CC_TGCA'GG
Cuts at: 2241 3155 2241
Size: 914 4889
ScaI AGT'ACT
Cuts at: 4051 5763 4051
Size: 1712 4091
SexAI A'CCwGG_T
Cuts at: 2193 2193
Size: 5803
SfcI C'TryA_G
Cuts at: 826 1045 2015 2024 2237 2413 3151 4286 4967
Size: 219 970 9 213 176 738 1135 681
Cuts at: 4967 5158 826
Size: 191 1471
Fragments arranged by size:
1471 1135 970 738 681 219 213 191
176 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 2186 2186
Size: 5803
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5803
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5803
SmaI CCC'GGG
Cuts at: 1872 3130 1872
Size: 1258 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3907 4808 5070 820
Size: 197 926 1964 901 262 1553
Fragments arranged by size:
1964 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3594 493
Size: 3101 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5803
SphI G_CATG'C
Cuts at: 1132 3161 1132
Size: 2029 3774
SspI AAT'ATT
Cuts at: 5 52 3727 5
Size: 47 3675 2081
Fragments arranged by size:
3675 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5803
StyI C'CwwG_G
Cuts at: 513 1065 2004 2154 2455 2547 2682 2724 3242
Size: 552 939 150 301 92 135 42 518
Cuts at: 3242 513
Size: 3074
Fragments arranged by size:
3074 939 552 518 301 150 135 92
42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2905 3830 3847 4000 4185 4630 325
Size: 1255 1325 925 17 153 185 445 1498
Fragments arranged by size:
1498 1325 1255 925 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4049 5761 96
Size: 274 80 33 51 1443 2072 1712 138
Fragments arranged by size:
2072 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5763 5795 5763
Size: 32 5771
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5803
XbaI T'CTAG_A
Cuts at: 3139 3139
Size: 5803
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5803
XmaI C'CCGG_G
Cuts at: 1870 3128 1870
Size: 1258 4545
XmnI GAAnn'nnTTC
Cuts at: 3932 3932
Size: 5803
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3611 276
Size: 53 83 186 936 2077 2468
Fragments arranged by size:
2468 2077 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SexAI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI
KasI NarI NruI PacI PfoI PmlI PspXI PsrI
RsrII SacII SanDI SfoI SrfI SwaI XcmI