Restriction map for
pFN21AB8323
(Circular) (Six-base) MAPSORT of: pFN21AB8323.seq Check: 2996 from: 1 to: 5464
pFN21AB8323 5464 bp
With 182 enzymes: SgfI *
February 27, 2009 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3274 278
Size: 53 83 186 936 1738 2468
Fragments arranged by size:
2468 1738 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2807 1937
Size: 870 4594
Acc65I G'GTAC_C
Cuts at: 2785 2785
Size: 5464
AclI AA'CG_TT
Cuts at: 1563 3591 3964 1563
Size: 2028 373 3063
Fragments arranged by size:
3063 2028 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2054 3527 4911 5179 1632
Size: 422 1473 1384 268 1917
Fragments arranged by size:
1917 1473 1384 422 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5267
AflIII A'CryG_T
Cuts at: 1203 2528 4363 5340 1203
Size: 1325 1835 977 1327
Fragments arranged by size:
1835 1327 1325 977
AgeI A'CCGG_T
Cuts at: 4295 4295
Size: 5464
AhdI GACnn_n'nnGTC
Cuts at: 4193 4193
Size: 5464
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5464
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2594 1184
Size: 1410 4054
AlwNI CAG_nnn'CTG
Cuts at: 2455 3207 4779 2455
Size: 752 1572 3140
Fragments arranged by size:
3140 1572 752
ApaLI G'TGCA_C
Cuts at: 3521 4677 3521
Size: 1156 4308
ApoI r'AATT_y
Cuts at: 1493 2282 2375 2395 2470 2534 2773 2999 3151
Size: 789 93 20 75 64 239 226 152
Cuts at: 3151 5260 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 789 239 226 152 93 75
64 20
AseI AT'TA_AT
Cuts at: 160 1032 4018 160
Size: 872 2986 1606
Fragments arranged by size:
2986 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5464
AvaI C'yCGr_G
Cuts at: 1870 1943 2216 2789 1870
Size: 73 273 573 4545
Fragments arranged by size:
4545 573 273 73
BamHI G'GATC_C
Cuts at: 2794 3161 2794
Size: 367 5097
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2785 4240 618
Size: 325 217 627 998 1455 1842
Fragments arranged by size:
1842 1455 998 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2055 2067 2783 1391
Size: 664 12 716 4072
Fragments arranged by size:
4072 716 664 12
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4493
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3655 3689 1903
Size: 34 1718 34 3678
Fragments arranged by size:
3678 1718 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3356 4566 3356
Size: 1210 4254
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5464
BfrBI ATG'CAT
Cuts at: 2675 2675
Size: 5464
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4075 136
Size: 107 122 71 631 605 2403 1525
Fragments arranged by size:
2403 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5459 5459
Size: 5464
BlpI GC'TnA_GC
Cuts at: 2881 2881
Size: 5464
Bme1580I G_kGCm'C
Cuts at: 1790 3525 4681 1790
Size: 1735 1156 2573
Fragments arranged by size:
2573 1735 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5464
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2703 3195 4153
Size: 416 53 83 399 24 1286 492 958
Cuts at: 4153 442
Size: 1753
Fragments arranged by size:
1753 1286 958 492 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5464
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2444 2677 4124 1239
Size: 1205 233 1447 2579
Fragments arranged by size:
2579 1447 1205 233
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3589 4454 4752 3589
Size: 865 298 4301
Fragments arranged by size:
4301 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2438 4127 882
Size: 617 939 1689 2219
Fragments arranged by size:
2219 1689 939 617
BsaAI yAC'GTr
Cuts at: 493 1204 2531 3255 493
Size: 711 1327 724 2702
Fragments arranged by size:
2702 1327 724 711
BsaBI GATnn'nnATC
Cuts at: 2463 5458 2463
Size: 2995 2469
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3271 3653 4288 275
Size: 53 83 186 936 1738 382 635 1451
Fragments arranged by size:
1738 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3897 4295 4569 4716 5127 3897
Size: 398 274 147 411 4234
Fragments arranged by size:
4234 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5239
Fragments arranged by size:
5239 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2054 2466 1186
Size: 868 412 4184
Fragments arranged by size:
4184 868 412
BseYI C'CCAG_C
Cuts at: 4667 5163 4667
Size: 496 4968
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4891
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2914 3675 4703 1540
Size: 275 186 913 761 1028 2301
Fragments arranged by size:
2301 1028 913 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2067 3525 3610 4681 2067
Size: 1458 85 1071 2850
Fragments arranged by size:
2850 1458 1071 85
BsmI GAATG_Cn'
Cuts at: 2975 3068 2975
Size: 93 5371
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2055 2067 2783 3525 3610 4681 1391
Size: 399 265 12 716 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 716 399 265 85 12
BspEI T'CCGG_A
Cuts at: 5127 5127
Size: 5464
BspHI T'CATG_A
Cuts at: 2130 3353 5083 2130
Size: 1223 1730 2511
Fragments arranged by size:
2511 1730 1223
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2208 2583 2819 844
Size: 1364 375 236 3489
Fragments arranged by size:
3489 1364 375 236
BsrBI CCG'CTC
Cuts at: 1394 1823 3351 1394
Size: 429 1528 3507
Fragments arranged by size:
3507 1528 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2554 3959 4133 65
Size: 1183 1306 1405 174 1396
Fragments arranged by size:
1405 1396 1306 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4108 4295 1700
Size: 110 2298 187 2869
Fragments arranged by size:
2869 2298 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5464
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5464
BssSI C'ACGA_G
Cuts at: 3524 4536 3524
Size: 1012 4452
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5464
BstBI TT'CG_AA
Cuts at: 4268 4268
Size: 5464
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5464
BstYI r'GATC_y
Cuts at: 1972 2794 3161 3546 3563 5015 5101 5113 5459
Size: 822 367 385 17 1452 86 12 346
Cuts at: 5459 1972
Size: 1977
Fragments arranged by size:
1977 1452 822 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3973
Fragments arranged by size:
3973 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4725
Fragments arranged by size:
4725 683 56
BtsI GCAGTG_nn'
Cuts at: 2089 2980 3774 3794 2089
Size: 891 794 20 3759
Fragments arranged by size:
3759 891 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5464
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5429
DraI TTT'AAA
Cuts at: 2768 2768
Size: 5464
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4471 809
Size: 507 3155 1802
Fragments arranged by size:
3155 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2607 2911 3800
Size: 54 1425 50 275 18 777 304 889
Cuts at: 3800 5429 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 777 304 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2911 1537
Size: 275 1099 4090
Fragments arranged by size:
4090 1099 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2072 2132 2286 3394 1353
Size: 719 60 154 1108 3423
Fragments arranged by size:
3423 1108 719 154 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4051 4425 4571 387
Size: 1064 138 2462 374 146 1280
Fragments arranged by size:
2462 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2065 2065
Size: 5464
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2054 2444 2677 3527 4124 4911 5179
Size: 393 422 390 233 850 597 787 268
Cuts at: 5179 1239
Size: 1524
Fragments arranged by size:
1524 850 787 597 422 393 390 268
233
EcoO109I rG'GnC_Cy
Cuts at: 2220 2220
Size: 5464
EcoRI G'AATT_C
Cuts at: 2395 2470 2773 3151 2395
Size: 75 303 378 4708
Fragments arranged by size:
4708 378 303 75
FspI TGC'GCA
Cuts at: 3970 3970
Size: 5464
HaeII r_GCGC'y
Cuts at: 1678 4611 1678
Size: 2933 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4105 4137 4179 4211 1373
Size: 32 135 32 2533 32 42 32 2626
Fragments arranged by size:
2626 2533 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2176 2808 3054 669
Size: 988 281 238 632 246 3079
Fragments arranged by size:
3079 988 632 281 246 238
HindIII A'AGCT_T
Cuts at: 748 2321 748
Size: 1573 3891
HpaI GTT'AAC
Cuts at: 3054 3054
Size: 5464
KpnI G_GTAC'C
Cuts at: 2789 2789
Size: 5464
MfeI C'AATT_G
Cuts at: 3063 3063
Size: 5464
MluI A'CGCG_T
Cuts at: 5340 5340
Size: 5464
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4578 4762 1305
Size: 3273 184 2007
Fragments arranged by size:
3273 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2609 10
Size: 54 1425 343 777 2865
Fragments arranged by size:
2865 1425 777 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3422 3781 3940 518
Size: 648 2256 359 159 2042
Fragments arranged by size:
2256 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2023 2880 3557 4705 4950 1606
Size: 417 857 677 1148 245 2120
Fragments arranged by size:
2120 1148 857 677 417 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3973
Fragments arranged by size:
3973 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5464
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5464
NotI GC'GGCC_GC
Cuts at: 2911 2911
Size: 5464
NsiI A_TGCA'T
Cuts at: 2677 2677
Size: 5464
NspI r_CATG'y
Cuts at: 1132 1229 2822 4367 1132
Size: 97 1593 1545 2229
Fragments arranged by size:
2229 1593 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5464
PciI A'CATG_T
Cuts at: 4363 4363
Size: 5464
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5464
PmeI GTTT'AAAC
Cuts at: 2768 2768
Size: 5464
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3533 3565 5046 5078 3533
Size: 32 1481 32 3919
Fragments arranged by size:
3919 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2220 2220
Size: 5464
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5464
PsiI TTA'TAA
Cuts at: 2237 3034 2237
Size: 797 4667
PstI C_TGCA'G
Cuts at: 830 2205 2451 2816 830
Size: 1375 246 365 3478
Fragments arranged by size:
3478 1375 365 246
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5464
SacI G_AGCT'C
Cuts at: 2067 2067
Size: 5464
SalI G'TCGA_C
Cuts at: 1936 2806 1936
Size: 870 4594
SanDI GG'GwC_CC
Cuts at: 2220 2220
Size: 5464
SapI GCTCTTCn'nnn_
Cuts at: 2072 2132 2072
Size: 60 5404
SbfI CC_TGCA'GG
Cuts at: 2816 2816
Size: 5464
ScaI AGT'ACT
Cuts at: 2315 3712 5424 2315
Size: 1397 1712 2355
Fragments arranged by size:
2355 1712 1397
SfcI C'TryA_G
Cuts at: 826 1045 2201 2447 2812 3947 4628 4819 826
Size: 219 1156 246 365 1135 681 191 1471
Fragments arranged by size:
1471 1156 1135 681 365 246 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5464
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5464
SmaI CCC'GGG
Cuts at: 1872 2218 2791 1872
Size: 346 573 4545
Fragments arranged by size:
4545 573 346
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3568 4469 4731 820
Size: 197 926 1625 901 262 1553
Fragments arranged by size:
1625 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3255 493
Size: 2762 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5464
SphI G_CATG'C
Cuts at: 1132 2822 1132
Size: 1690 3774
SspI AAT'ATT
Cuts at: 5 52 3388 5
Size: 47 3336 2081
Fragments arranged by size:
3336 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5464
StyI C'CwwG_G
Cuts at: 513 1065 2004 2760 2903 513
Size: 552 939 756 143 3074
Fragments arranged by size:
3074 939 756 552 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2241 2352 3491 3508 3661 3846 4291
Size: 1255 661 111 1139 17 153 185 445
Cuts at: 4291 325
Size: 1498
Fragments arranged by size:
1498 1255 1139 661 445 185 153 111
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2313 3710 5422
Size: 274 80 33 51 1443 336 1397 1712
Cuts at: 5422 96
Size: 138
Fragments arranged by size:
1712 1443 1397 336 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5424 5456 5424
Size: 32 5432
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5464
XbaI T'CTAG_A
Cuts at: 2800 2800
Size: 5464
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5464
XmaI C'CCGG_G
Cuts at: 1870 2216 2789 1870
Size: 346 573 4545
Fragments arranged by size:
4545 573 346
XmnI GAAnn'nnTTC
Cuts at: 3593 3593
Size: 5464
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3272 276
Size: 53 83 186 936 1738 2468
Fragments arranged by size:
2468 1738 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
SacI SalI SanDI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI
PsrI PvuII RsrII SacII SexAI SfiI SfoI SrfI
SwaI XcmI