Restriction map for
pFN21AB8321
(Circular) (Six-base) MAPSORT of: pFN21AB8321.seq Check: 8603 from: 1 to: 5581
pFN21AB8321 5581 bp
With 182 enzymes: SgfI *
February 27, 2009 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3391 278
Size: 53 83 186 936 1855 2468
Fragments arranged by size:
2468 1855 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2924 1937
Size: 987 4594
Acc65I G'GTAC_C
Cuts at: 2902 2902
Size: 5581
AclI AA'CG_TT
Cuts at: 1563 3708 4081 1563
Size: 2145 373 3063
Fragments arranged by size:
3063 2145 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2615 3644 5028 5296 1632
Size: 983 1029 1384 268 1917
Fragments arranged by size:
1917 1384 1029 983 268
AflII C'TTAA_G
Cuts at: 820 1017 2618 820
Size: 197 1601 3783
Fragments arranged by size:
3783 1601 197
AflIII A'CryG_T
Cuts at: 1203 2322 4480 5457 1203
Size: 1119 2158 977 1327
Fragments arranged by size:
2158 1327 1119 977
AgeI A'CCGG_T
Cuts at: 4412 4412
Size: 5581
AhdI GACnn_n'nnGTC
Cuts at: 4310 4310
Size: 5581
AleI CACnn'nnGTG
Cuts at: 1166 2798 1166
Size: 1632 3949
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5581
AlwNI CAG_nnn'CTG
Cuts at: 2197 3324 4896 2197
Size: 1127 1572 2882
Fragments arranged by size:
2882 1572 1127
ApaLI G'TGCA_C
Cuts at: 3638 4794 3638
Size: 1156 4425
ApoI r'AATT_y
Cuts at: 1493 2129 2162 2303 2543 2890 3116 3268 5377
Size: 636 33 141 240 347 226 152 2109
Cuts at: 5377 1493
Size: 1697
Fragments arranged by size:
2109 1697 636 347 240 226 152 141
33
AseI AT'TA_AT
Cuts at: 160 1032 4135 160
Size: 872 3103 1606
Fragments arranged by size:
3103 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5581
AvaI C'yCGr_G
Cuts at: 1870 1943 2906 1870
Size: 73 963 4545
Fragments arranged by size:
4545 963 73
BamHI G'GATC_C
Cuts at: 2911 3278 2911
Size: 367 5214
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2902 4357 618
Size: 325 217 627 1115 1455 1842
Fragments arranged by size:
1842 1455 1115 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2900 1391
Size: 1509 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2621 928
Size: 971 722 3888
Fragments arranged by size:
3888 971 722
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3772 3806 1903
Size: 34 50 34 1751 34 3678
Fragments arranged by size:
3678 1751 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2277 3473 4683 2277
Size: 1196 1210 3175
Fragments arranged by size:
3175 1210 1196
BclI T'GATC_A
Cuts at: 1548 2644 1548
Size: 1096 4485
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4192 136
Size: 107 122 71 631 605 2520 1525
Fragments arranged by size:
2520 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2344 5576 2344
Size: 3232 2349
BlpI GC'TnA_GC
Cuts at: 2998 2998
Size: 5581
Bme1580I G_kGCm'C
Cuts at: 1790 3642 4798 1790
Size: 1852 1156 2573
Fragments arranged by size:
2573 1852 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5581
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3312 4270 442
Size: 416 53 83 399 24 1895 958 1753
Fragments arranged by size:
1895 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5581
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2459 2459
Size: 5581
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4241 1239
Size: 3002 2579
Bpu10I CC'TnA_GC
Cuts at: 2788 2788
Size: 5581
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3706 4571 4869 3706
Size: 865 298 4418
Fragments arranged by size:
4418 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4244 882
Size: 617 2745 2219
Fragments arranged by size:
2745 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3372 493
Size: 711 2168 2702
Fragments arranged by size:
2702 2168 711
BsaBI GATnn'nnATC
Cuts at: 2849 5575 2849
Size: 2726 2855
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3388 3770 4405 275
Size: 53 83 186 936 1855 382 635 1451
Fragments arranged by size:
1855 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4014 4412 4686 4833 5244 4014
Size: 398 274 147 411 4351
Fragments arranged by size:
4351 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2312 2342 1178
Size: 30 165 30 909 30 4417
Fragments arranged by size:
4417 909 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5581
BseYI C'CCAG_C
Cuts at: 2233 4784 5280 2233
Size: 2551 496 2534
Fragments arranged by size:
2551 2534 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2390 1163
Size: 573 654 4354
Fragments arranged by size:
4354 654 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3031 3792 4820 1540
Size: 275 186 1030 761 1028 2301
Fragments arranged by size:
2301 1030 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2100 3642 3727 4798 2100
Size: 1542 85 1071 2883
Fragments arranged by size:
2883 1542 1071 85
BsmI GAATG_Cn'
Cuts at: 3092 3185 3092
Size: 93 5488
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2100 2900 3642 3727 4798 1391
Size: 399 310 800 742 85 1071 2174
Fragments arranged by size:
2174 1071 800 742 399 310 85
BspEI T'CCGG_A
Cuts at: 5244 5244
Size: 5581
BspHI T'CATG_A
Cuts at: 3470 5200 3470
Size: 1730 3851
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2869 2936 844
Size: 2025 67 3489
Fragments arranged by size:
3489 2025 67
BsrBI CCG'CTC
Cuts at: 1394 1823 3468 1394
Size: 429 1645 3507
Fragments arranged by size:
3507 1645 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2212 4076 4250 65
Size: 1183 964 1864 174 1396
Fragments arranged by size:
1864 1396 1183 964 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4225 4412 1700
Size: 110 2415 187 2869
Fragments arranged by size:
2869 2415 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5581
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5581
BssSI C'ACGA_G
Cuts at: 3641 4653 3641
Size: 1012 4569
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5581
BstBI TT'CG_AA
Cuts at: 4385 4385
Size: 5581
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5581
BstYI r'GATC_y
Cuts at: 1972 2082 2344 2911 3278 3663 3680 5132 5218
Size: 110 262 567 367 385 17 1452 86
Cuts at: 5218 5230 5576 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 567 385 367 346 262 110
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2118 513
Size: 552 1053 3976
Fragments arranged by size:
3976 1053 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4842
Fragments arranged by size:
4842 683 56
BtsI GCAGTG_nn'
Cuts at: 2402 2696 3097 3891 3911 2402
Size: 294 401 794 20 4072
Fragments arranged by size:
4072 794 401 294 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5581
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2752 2787 529
Size: 35 2188 35 3323
Fragments arranged by size:
3323 2188 35 35
DraI TTT'AAA
Cuts at: 2220 2885 2220
Size: 665 4916
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4588 809
Size: 507 3272 1802
Fragments arranged by size:
3272 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3028 3917 5546
Size: 54 1425 50 275 18 1198 889 1629
Cuts at: 5546 8
Size: 43
Fragments arranged by size:
1629 1425 1198 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3028 1537
Size: 275 1216 4090
Fragments arranged by size:
4090 1216 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2064 2379 2526 2597 3511 1353
Size: 711 315 147 71 914 3423
Fragments arranged by size:
3423 914 711 315 147 71
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4168 4542 4688 387
Size: 1064 138 2579 374 146 1280
Fragments arranged by size:
2579 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2615 3644 4241 5028 5296 1239
Size: 393 983 1029 597 787 268 1524
Fragments arranged by size:
1524 1029 983 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2315 2315
Size: 5581
EcoRI G'AATT_C
Cuts at: 2543 2890 3268 2543
Size: 347 378 4856
Fragments arranged by size:
4856 378 347
FspI TGC'GCA
Cuts at: 4087 4087
Size: 5581
HaeII r_GCGC'y
Cuts at: 1678 4728 1678
Size: 3050 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2312 2344 2813 2845 4222
Size: 32 135 32 740 32 469 32 1377
Cuts at: 4222 4254 4296 4328 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1377 740 469 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2212 2925 3171 669
Size: 988 281 274 713 246 3079
Fragments arranged by size:
3079 988 713 281 274 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5581
HpaI GTT'AAC
Cuts at: 3171 3171
Size: 5581
KpnI G_GTAC'C
Cuts at: 2906 2906
Size: 5581
MfeI C'AATT_G
Cuts at: 2154 3180 2154
Size: 1026 4555
MluI A'CGCG_T
Cuts at: 5457 5457
Size: 5581
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2631 4695 4879 1305
Size: 1326 2064 184 2007
Fragments arranged by size:
2064 2007 1326 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3759
Fragments arranged by size:
3759 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2798 3539 3898 4057 518
Size: 648 1632 741 359 159 2042
Fragments arranged by size:
2042 1632 741 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2233 2997 3674 4822 5067 1606
Size: 627 764 677 1148 245 2120
Fragments arranged by size:
2120 1148 764 677 627 245
NcoI C'CATG_G
Cuts at: 513 1065 2118 513
Size: 552 1053 3976
Fragments arranged by size:
3976 1053 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5581
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5581
NotI GC'GGCC_GC
Cuts at: 3028 3028
Size: 5581
NspI r_CATG'y
Cuts at: 1132 1229 2326 2875 2939 4484 1132
Size: 97 1097 549 64 1545 2229
Fragments arranged by size:
2229 1545 1097 549 97 64
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5581
PciI A'CATG_T
Cuts at: 2322 4480 2322
Size: 2158 3423
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5581
PmeI GTTT'AAAC
Cuts at: 2885 2885
Size: 5581
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3650 3682 5163 5195 3650
Size: 32 1481 32 4036
Fragments arranged by size:
4036 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5581
PsiI TTA'TAA
Cuts at: 3151 3151
Size: 5581
PstI C_TGCA'G
Cuts at: 830 2014 2933 830
Size: 1184 919 3478
Fragments arranged by size:
3478 1184 919
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5581
PvuII CAG'CTG
Cuts at: 2233 2233
Size: 5581
SalI G'TCGA_C
Cuts at: 1936 2923 1936
Size: 987 4594
SapI GCTCTTCn'nnn_
Cuts at: 2597 2597
Size: 5581
SbfI CC_TGCA'GG
Cuts at: 2933 2933
Size: 5581
ScaI AGT'ACT
Cuts at: 2768 3829 5541 2768
Size: 1061 1712 2808
Fragments arranged by size:
2808 1712 1061
SfcI C'TryA_G
Cuts at: 826 1045 2010 2366 2929 4064 4745 4936 826
Size: 219 965 356 563 1135 681 191 1471
Fragments arranged by size:
1471 1135 965 681 563 356 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5581
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5581
SmaI CCC'GGG
Cuts at: 1872 2908 1872
Size: 1036 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2618 3685 4586 4848 820
Size: 197 926 675 1067 901 262 1553
Fragments arranged by size:
1553 1067 926 901 675 262 197
SnaBI TAC'GTA
Cuts at: 493 3372 493
Size: 2879 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5581
SphI G_CATG'C
Cuts at: 1132 2939 1132
Size: 1807 3774
SspI AAT'ATT
Cuts at: 5 52 3505 5
Size: 47 3453 2081
Fragments arranged by size:
3453 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5581
StyI C'CwwG_G
Cuts at: 513 1065 2118 3020 513
Size: 552 1053 902 3074
Fragments arranged by size:
3074 1053 902 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3608 3625 3778 3963 4408 325
Size: 1255 2028 17 153 185 445 1498
Fragments arranged by size:
2028 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2325 2766 3827
Size: 274 80 33 51 1443 348 441 1061
Cuts at: 3827 5539 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1061 441 348 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2116 2148 5541 5573 2116
Size: 32 3393 32 2124
Fragments arranged by size:
3393 2124 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2453 1316
Size: 1137 4444
XbaI T'CTAG_A
Cuts at: 2062 2917 2062
Size: 855 4726
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5581
XmaI C'CCGG_G
Cuts at: 1870 2906 1870
Size: 1036 4545
XmnI GAAnn'nnTTC
Cuts at: 2547 2564 3710 2547
Size: 17 1146 4418
Fragments arranged by size:
4418 1146 17
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3389 276
Size: 53 83 186 936 1855 2468
Fragments arranged by size:
2468 1855 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI
SfiI SfoI SrfI SwaI XcmI