Restriction map for
pFN21AB8317
(Circular) (Six-base) MAPSORT of: pFN21AB8317.seq Check: 9613 from: 1 to: 6163
pFN21AB8317 6163 bp
With 182 enzymes: SgfI *
February 27, 2009 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2617 2617
Size: 6163
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3973 278
Size: 53 83 186 936 2437 2468
Fragments arranged by size:
2468 2437 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3506 1937
Size: 1569 4594
Acc65I G'GTAC_C
Cuts at: 3484 3484
Size: 6163
AclI AA'CG_TT
Cuts at: 1563 4290 4663 1563
Size: 2727 373 3063
Fragments arranged by size:
3063 2727 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2328 3032 4226 5610 5878 1632
Size: 696 704 1194 1384 268 1917
Fragments arranged by size:
1917 1384 1194 704 696 268
AflII C'TTAA_G
Cuts at: 820 1017 2327 820
Size: 197 1310 4656
Fragments arranged by size:
4656 1310 197
AflIII A'CryG_T
Cuts at: 1203 5062 6039 1203
Size: 3859 977 1327
Fragments arranged by size:
3859 1327 977
AgeI A'CCGG_T
Cuts at: 4994 4994
Size: 6163
AhdI GACnn_n'nnGTC
Cuts at: 4892 4892
Size: 6163
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6163
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6163
AlwNI CAG_nnn'CTG
Cuts at: 2555 2840 3240 3906 5478 2555
Size: 285 400 666 1572 3240
Fragments arranged by size:
3240 1572 666 400 285
ApaLI G'TGCA_C
Cuts at: 4220 5376 4220
Size: 1156 5007
ApoI r'AATT_y
Cuts at: 1493 3472 3698 3850 5959 1493
Size: 1979 226 152 2109 1697
Fragments arranged by size:
2109 1979 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4717 160
Size: 872 3685 1606
Fragments arranged by size:
3685 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6163
AvaI C'yCGr_G
Cuts at: 1870 1943 2928 3182 3488 1870
Size: 73 985 254 306 4545
Fragments arranged by size:
4545 985 306 254 73
BamHI G'GATC_C
Cuts at: 3493 3860 3493
Size: 367 5796
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2588 3484 4939 618
Size: 325 217 627 801 896 1455 1842
Fragments arranged by size:
1842 1455 896 801 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2028 2139 2172 2498 3029 3183 3191 3219
Size: 637 111 33 326 531 154 8 28
Cuts at: 3219 3482 1391
Size: 263 4072
Fragments arranged by size:
4072 637 531 326 263 154 111 33
28 8
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2052 2868 3020 928
Size: 971 153 816 152 4071
Fragments arranged by size:
4071 971 816 153 152
BbvCI CC'TCA_GC
Cuts at: 2706 2775 2706
Size: 69 6094
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4354 4388 1903
Size: 34 2417 34 3678
Fragments arranged by size:
3678 2417 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4055 5265 4055
Size: 1210 4953
BclI T'GATC_A
Cuts at: 1548 2376 2745 1548
Size: 828 369 4966
Fragments arranged by size:
4966 828 369
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2849 3338 4774
Size: 107 122 71 631 605 1177 489 1436
Cuts at: 4774 136
Size: 1525
Fragments arranged by size:
1525 1436 1177 631 605 489 122 107
71
BglII A'GATC_T
Cuts at: 6158 6158
Size: 6163
BlpI GC'TnA_GC
Cuts at: 2166 3580 2166
Size: 1414 4749
Bme1580I G_kGCm'C
Cuts at: 1790 4224 5380 1790
Size: 2434 1156 2573
Fragments arranged by size:
2573 2434 1156
BmgBI CAC'GTC
Cuts at: 1318 2613 1318
Size: 1295 4868
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2493 3894 4852
Size: 416 53 83 399 24 1076 1401 958
Cuts at: 4852 442
Size: 1753
Fragments arranged by size:
1753 1401 1076 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6163
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2042 2532 2663 3296 4823 1239
Size: 803 490 131 633 1527 2579
Fragments arranged by size:
2579 1527 803 633 490 131
Bpu10I CC'TnA_GC
Cuts at: 2027 2706 2775 3177 3213 2027
Size: 679 69 402 36 4977
Fragments arranged by size:
4977 679 402 69 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2051 2066 3247 4288 5153 5451 2051
Size: 15 1181 1041 865 298 2763
Fragments arranged by size:
2763 1181 1041 865 298 15
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2590 2891 4826 882
Size: 617 1091 301 1935 2219
Fragments arranged by size:
2219 1935 1091 617 301
BsaAI yAC'GTr
Cuts at: 493 1204 3954 493
Size: 711 2750 2702
Fragments arranged by size:
2750 2702 711
BsaBI GATnn'nnATC
Cuts at: 6157 6157
Size: 6163
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2572 3440 3970 4352
Size: 53 83 186 936 1039 868 530 382
Cuts at: 4352 4987 275
Size: 635 1451
Fragments arranged by size:
1451 1039 936 868 635 530 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4596 4994 5268 5415 5826 4596
Size: 398 274 147 411 4933
Fragments arranged by size:
4933 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5938
Fragments arranged by size:
5938 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3070 3263 3279 3434 1186
Size: 1884 193 16 155 3915
Fragments arranged by size:
3915 1884 193 155 16
BseYI C'CCAG_C
Cuts at: 2012 2354 5366 5862 2012
Size: 342 3012 496 2313
Fragments arranged by size:
3012 2313 496 342
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2162 2645 2912 1163
Size: 573 426 483 267 4414
Fragments arranged by size:
4414 573 483 426 267
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3342 3613 4374 5402 1540
Size: 275 186 1341 271 761 1028 2301
Fragments arranged by size:
2301 1341 1028 761 275 271 186
BsiHKAI G_wGCw'C
Cuts at: 3102 3191 4224 4309 5380 3102
Size: 89 1033 85 1071 3885
Fragments arranged by size:
3885 1071 1033 89 85
BsmI GAATG_Cn'
Cuts at: 3674 3767 3674
Size: 93 6070
BsmBI CGTCTCn'nnnn_
Cuts at: 2927 2927
Size: 6163
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2028 2139 2172 2498 3029 3102 3183
Size: 399 238 111 33 326 531 73 81
Cuts at: 3183 3191 3219 3482 4224 4309 5380 1391
Size: 8 28 263 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 531 399 326 263 238
111 85 81 73 33 28 8
BspEI T'CCGG_A
Cuts at: 5826 5826
Size: 6163
BspHI T'CATG_A
Cuts at: 4052 5782 4052
Size: 1730 4433
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2617 3518 844
Size: 1773 901 3489
Fragments arranged by size:
3489 1773 901
BsrBI CCG'CTC
Cuts at: 1394 1823 2349 2586 3000 4050 1394
Size: 429 526 237 414 1050 3507
Fragments arranged by size:
3507 1050 526 429 414 237
BsrDI GCAATG_nn'
Cuts at: 65 1248 4658 4832 65
Size: 1183 3410 174 1396
Fragments arranged by size:
3410 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4807 4994 1700
Size: 110 2997 187 2869
Fragments arranged by size:
2997 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6163
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6163
BssSI C'ACGA_G
Cuts at: 2593 4223 5235 2593
Size: 1630 1012 3521
Fragments arranged by size:
3521 1630 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2840 3240 794
Size: 2046 400 3717
Fragments arranged by size:
3717 2046 400
BstBI TT'CG_AA
Cuts at: 2430 4967 2430
Size: 2537 3626
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6163
BstYI r'GATC_y
Cuts at: 1972 3493 3860 4245 4262 5714 5800 5812 6158
Size: 1521 367 385 17 1452 86 12 346
Cuts at: 6158 1972
Size: 1977
Fragments arranged by size:
1977 1521 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2787 2787
Size: 6163
BtgI C'CryG_G
Cuts at: 513 1065 2004 3318 3336 513
Size: 552 939 1314 18 3340
Fragments arranged by size:
3340 1314 939 552 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5424
Fragments arranged by size:
5424 683 56
BtsI GCAGTG_nn'
Cuts at: 3146 3679 4473 4493 3146
Size: 533 794 20 4816
Fragments arranged by size:
4816 794 533 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6163
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6128
DraI TTT'AAA
Cuts at: 2089 3467 2089
Size: 1378 4785
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5170 809
Size: 507 3854 1802
Fragments arranged by size:
3854 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2344 2607 3339
Size: 54 1425 50 275 18 514 263 732
Cuts at: 3339 3610 4499 6128 8
Size: 271 889 1629 43
Fragments arranged by size:
1629 1425 889 732 514 275 271 263
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3339 3610 1537
Size: 275 1527 271 4090
Fragments arranged by size:
4090 1527 275 271
EarI CTCTTCn'nnn_
Cuts at: 1353 2109 2990 4093 1353
Size: 756 881 1103 3423
Fragments arranged by size:
3423 1103 881 756
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2203 3049 4750 5124 5270 387
Size: 1064 138 614 846 1701 374 146 1280
Fragments arranged by size:
1701 1280 1064 846 614 374 146 138
EcoICRI GAG'CTC
Cuts at: 3189 3189
Size: 6163
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2042 2328 2532 2663 3032 3296 4226
Size: 393 410 286 204 131 369 264 930
Cuts at: 4226 4823 5610 5878 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 930 787 597 410 393 369 286
268 264 204 131
EcoO109I rG'GnC_Cy
Cuts at: 2053 2062 2279 2638 3136 3196 2053
Size: 9 217 359 498 60 5020
Fragments arranged by size:
5020 498 359 217 60 9
EcoRI G'AATT_C
Cuts at: 3472 3850 3472
Size: 378 5785
FspI TGC'GCA
Cuts at: 4669 4669
Size: 6163
HaeII r_GCGC'y
Cuts at: 1678 2260 3078 5310 1678
Size: 582 818 2232 2531
Fragments arranged by size:
2531 2232 818 582
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4804 4836 4878 4910 1373
Size: 32 135 32 3232 32 42 32 2626
Fragments arranged by size:
3232 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3507 3753 669
Size: 988 281 1569 246 3079
Fragments arranged by size:
3079 1569 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2232 2324 748
Size: 1484 92 4587
Fragments arranged by size:
4587 1484 92
HpaI GTT'AAC
Cuts at: 3753 3753
Size: 6163
KpnI G_GTAC'C
Cuts at: 3488 3488
Size: 6163
MfeI C'AATT_G
Cuts at: 3762 3762
Size: 6163
MluI A'CGCG_T
Cuts at: 6039 6039
Size: 6163
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5277 5461 1305
Size: 3972 184 2007
Fragments arranged by size:
3972 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4341
Fragments arranged by size:
4341 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4121 4480 4639 518
Size: 648 2955 359 159 2042
Fragments arranged by size:
2955 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2254 2840 3320 3579 4256 5404 5649 1606
Size: 648 586 480 259 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 648 586 480 259 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4672
Fragments arranged by size:
4672 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6163
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6163
NotI GC'GGCC_GC
Cuts at: 3610 3610
Size: 6163
NspI r_CATG'y
Cuts at: 1132 1229 3521 5066 1132
Size: 97 2292 1545 2229
Fragments arranged by size:
2292 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2386 3029 1348
Size: 1038 643 4482
Fragments arranged by size:
4482 1038 643
PciI A'CATG_T
Cuts at: 5062 5062
Size: 6163
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2555 2740 1108
Size: 1447 185 4531
Fragments arranged by size:
4531 1447 185
PfoI T'CCnGG_A
Cuts at: 2548 2640 2548
Size: 92 6071
PmeI GTTT'AAAC
Cuts at: 3467 3467
Size: 6163
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4232 4264 5745 5777 4232
Size: 32 1481 32 4618
Fragments arranged by size:
4618 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2053 2279 2638 3196 2053
Size: 226 359 558 5020
Fragments arranged by size:
5020 558 359 226
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6163
PsiI TTA'TAA
Cuts at: 3733 3733
Size: 6163
PstI C_TGCA'G
Cuts at: 830 3515 830
Size: 2685 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6163
SacI G_AGCT'C
Cuts at: 3191 3191
Size: 6163
SacII CC_GC'GG
Cuts at: 3321 3321
Size: 6163
SalI G'TCGA_C
Cuts at: 1936 3505 1936
Size: 1569 4594
SanDI GG'GwC_CC
Cuts at: 2279 2279
Size: 6163
SapI GCTCTTCn'nnn_
Cuts at: 2990 2990
Size: 6163
SbfI CC_TGCA'GG
Cuts at: 3515 3515
Size: 6163
ScaI AGT'ACT
Cuts at: 4411 6123 4411
Size: 1712 4451
SfcI C'TryA_G
Cuts at: 826 1045 3511 4646 5327 5518 826
Size: 219 2466 1135 681 191 1471
Fragments arranged by size:
2466 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6163
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6163
SmaI CCC'GGG
Cuts at: 1872 2930 3490 1872
Size: 1058 560 4545
Fragments arranged by size:
4545 1058 560
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2066 2081 2327 3226 4267 5168
Size: 197 926 123 15 246 899 1041 901
Cuts at: 5168 5430 820
Size: 262 1553
Fragments arranged by size:
1553 1041 926 901 899 262 246 197
123 15
SnaBI TAC'GTA
Cuts at: 493 3954 493
Size: 3461 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6163
SphI G_CATG'C
Cuts at: 1132 3521 1132
Size: 2389 3774
SspI AAT'ATT
Cuts at: 5 52 4087 5
Size: 47 4035 2081
Fragments arranged by size:
4035 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6163
StyI C'CwwG_G
Cuts at: 513 1065 2004 2601 3057 3294 3404 3602 513
Size: 552 939 597 456 237 110 198 3074
Fragments arranged by size:
3074 939 597 552 456 237 198 110
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2747 3464 4190 4207 4360 4545 4990
Size: 1255 1167 717 726 17 153 185 445
Cuts at: 4990 325
Size: 1498
Fragments arranged by size:
1498 1255 1167 726 717 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3351 4409 6121
Size: 274 80 33 51 1443 1374 1058 1712
Cuts at: 6121 96
Size: 138
Fragments arranged by size:
1712 1443 1374 1058 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3110 3142 6123 6155 3110
Size: 32 2981 32 3118
Fragments arranged by size:
3118 2981 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6163
XbaI T'CTAG_A
Cuts at: 3499 3499
Size: 6163
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2737 2737
Size: 6163
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6163
XmaI C'CCGG_G
Cuts at: 1870 2928 3488 1870
Size: 1058 560 4545
Fragments arranged by size:
4545 1058 560
XmnI GAAnn'nnTTC
Cuts at: 4292 4292
Size: 6163
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3971 276
Size: 53 83 186 936 2437 2468
Fragments arranged by size:
2468 2437 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstXI BstZ17I DraIII EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI PvuII RsrII SexAI
SfiI SfoI SrfI SwaI