Restriction map for
pFN21AB8316
(Circular) (Six-base) MAPSORT of: pFN21AB8316.seq Check: 5049 from: 1 to: 5974
pFN21AB8316 5974 bp
With 182 enzymes: SgfI *
August 16, 2010 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2262 2424 3784 278
Size: 53 83 186 936 726 162 1360 2468
Fragments arranged by size:
2468 1360 936 726 186 162 83 53
AccI GT'mk_AC
Cuts at: 1937 3317 1937
Size: 1380 4594
Acc65I G'GTAC_C
Cuts at: 3103 3295 3103
Size: 192 5782
AclI AA'CG_TT
Cuts at: 1563 4101 4474 1563
Size: 2538 373 3063
Fragments arranged by size:
3063 2538 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4037 5421 5689 1632
Size: 2405 1384 268 1917
Fragments arranged by size:
2405 1917 1384 268
AfeI AGC'GCT
Cuts at: 2955 2955
Size: 5974
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5777
AflIII A'CryG_T
Cuts at: 1203 2733 4873 5850 1203
Size: 1530 2140 977 1327
Fragments arranged by size:
2140 1530 1327 977
AgeI A'CCGG_T
Cuts at: 3106 4805 3106
Size: 1699 4275
AhdI GACnn_n'nnGTC
Cuts at: 2158 4703 2158
Size: 2545 3429
AleI CACnn'nnGTG
Cuts at: 1166 2927 1166
Size: 1761 4213
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5974
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2216 2248 2216
Size: 32 5942
AlwNI CAG_nnn'CTG
Cuts at: 2311 2687 2717 3204 3717 5289 2311
Size: 376 30 487 513 1572 2996
Fragments arranged by size:
2996 1572 513 487 376 30
ApaI G_GGCC'C
Cuts at: 3240 3240
Size: 5974
ApaLI G'TGCA_C
Cuts at: 4031 5187 4031
Size: 1156 4818
ApoI r'AATT_y
Cuts at: 1493 2050 2995 3283 3509 3661 5770 1493
Size: 557 945 288 226 152 2109 1697
Fragments arranged by size:
2109 1697 945 557 288 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4528 160
Size: 872 3496 1606
Fragments arranged by size:
3496 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5974
AvaI C'yCGr_G
Cuts at: 1870 1943 2155 3299 1870
Size: 73 212 1144 4545
Fragments arranged by size:
4545 1144 212 73
BamHI G'GATC_C
Cuts at: 2693 3304 3671 2693
Size: 611 367 4996
Fragments arranged by size:
4996 611 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2083 2704 2839 3103 3295
Size: 325 217 627 296 621 135 264 192
Cuts at: 3295 4750 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 621 325 296 264 217
192 135
BanII G_rGCy'C
Cuts at: 1391 2174 3240 3293 1391
Size: 783 1066 53 4072
Fragments arranged by size:
4072 1066 783 53
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5003
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4165 4199 1903
Size: 34 2228 34 3678
Fragments arranged by size:
3678 2228 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3866 5076 3866
Size: 1210 4764
BclI T'GATC_A
Cuts at: 1548 2379 1548
Size: 831 5143
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4585 136
Size: 107 122 71 631 605 2913 1525
Fragments arranged by size:
2913 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5969 5969
Size: 5974
BlpI GC'TnA_GC
Cuts at: 3391 3391
Size: 5974
Bme1580I G_kGCm'C
Cuts at: 1790 3240 4035 5191 1790
Size: 1450 795 1156 2573
Fragments arranged by size:
2573 1450 1156 795
BmgBI CAC'GTC
Cuts at: 1318 2736 2920 3210 1318
Size: 1418 184 290 4082
Fragments arranged by size:
4082 1418 290 184
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2092 2175 3705
Size: 416 53 83 399 24 675 83 1530
Cuts at: 3705 4663 442
Size: 958 1753
Fragments arranged by size:
1753 1530 958 675 416 399 83 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3231 1056
Size: 2175 3799
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2049 2049
Size: 5974
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4634 1239
Size: 3395 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2200 4099 4964 5262 2200
Size: 1899 865 298 2912
Fragments arranged by size:
2912 1899 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2105 2557 4637 882
Size: 617 606 452 2080 2219
Fragments arranged by size:
2219 2080 617 606 452
BsaAI yAC'GTr
Cuts at: 493 1204 3765 493
Size: 711 2561 2702
Fragments arranged by size:
2702 2561 711
BsaBI GATnn'nnATC
Cuts at: 5968 5968
Size: 5974
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2259 2421 2500 3781
Size: 53 83 186 936 726 162 79 1281
Cuts at: 3781 4163 4798 275
Size: 382 635 1451
Fragments arranged by size:
1451 1281 936 726 635 382 186 162
83 79 53
BsaWI w'CCGG_w
Cuts at: 2926 3106 4407 4805 5079 5226 5637 2926
Size: 180 1301 398 274 147 411 3263
Fragments arranged by size:
3263 1301 411 398 274 180 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5749
Fragments arranged by size:
5749 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2276 2606 2684 1186
Size: 1090 330 78 4476
Fragments arranged by size:
4476 1090 330 78
BseYI C'CCAG_C
Cuts at: 2125 2880 3125 3172 5177 5673 2125
Size: 755 245 47 2005 496 2426
Fragments arranged by size:
2426 2005 755 496 245 47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2637 3054 3117 3164 3190 1163
Size: 573 901 417 63 47 26 3947
Fragments arranged by size:
3947 901 573 417 63 47 26
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2784 2889 3424 4185 5213 1540
Size: 275 186 783 105 535 761 1028 2301
Fragments arranged by size:
2301 1028 783 761 535 275 186 105
BsiHKAI G_wGCw'C
Cuts at: 3272 4035 4120 5191 3272
Size: 763 85 1071 4055
Fragments arranged by size:
4055 1071 763 85
BsmI GAATG_Cn'
Cuts at: 3485 3578 3485
Size: 93 5881
BsmBI CGTCTCn'nnnn_
Cuts at: 2742 2742
Size: 5974
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2174 3240 3272 3293 4035 4120 5191
Size: 399 384 1066 32 21 742 85 1071
Cuts at: 5191 1391
Size: 2174
Fragments arranged by size:
2174 1071 1066 742 399 384 85 32
21
BspEI T'CCGG_A
Cuts at: 5637 5637
Size: 5974
BspHI T'CATG_A
Cuts at: 3863 5593 3863
Size: 1730 4244
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3329 844
Size: 2485 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3861 1394
Size: 429 2038 3507
Fragments arranged by size:
3507 2038 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3187 4469 4643 65
Size: 1183 1939 1282 174 1396
Fragments arranged by size:
1939 1396 1282 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2779 2783 2884 2888 3106 4618 4805
Size: 110 969 4 101 4 218 1512 187
Cuts at: 4805 1700
Size: 2869
Fragments arranged by size:
2869 1512 969 218 187 110 101 4
4
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5974
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5974
BssSI C'ACGA_G
Cuts at: 2618 4034 5046 2618
Size: 1416 1012 3546
Fragments arranged by size:
3546 1416 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3140 794
Size: 2346 3628
BstBI TT'CG_AA
Cuts at: 4778 4778
Size: 5974
BstEII G'GTnAC_C
Cuts at: 1184 3109 1184
Size: 1925 4049
BstYI r'GATC_y
Cuts at: 1972 2131 2693 2978 3304 3671 4056 4073 5525
Size: 159 562 285 326 367 385 17 1452
Cuts at: 5525 5611 5623 5969 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 562 385 367 346 326 285
159 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2853 3048 3150 513
Size: 552 939 849 195 102 3337
Fragments arranged by size:
3337 939 849 552 195 102
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3246 487
Size: 683 56 2020 3215
Fragments arranged by size:
3215 2020 683 56
BtsI GCAGTG_nn'
Cuts at: 3202 3490 4284 4304 3202
Size: 288 794 20 4872
Fragments arranged by size:
4872 794 288 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5974
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5939
DraI TTT'AAA
Cuts at: 3278 3278
Size: 5974
DraIII CAC_nnn'GTG
Cuts at: 2434 2434
Size: 5974
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2258 4981 809
Size: 507 942 2723 1802
Fragments arranged by size:
2723 1802 942 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2217 2781 2886
Size: 54 1425 50 275 18 387 564 105
Cuts at: 2886 2931 3421 4310 5939 8
Size: 45 490 889 1629 43
Fragments arranged by size:
1629 1425 889 564 490 387 275 105
54 50 45 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2781 2886 3421 1537
Size: 275 969 105 535 4090
Fragments arranged by size:
4090 969 535 275 105
EarI CTCTTCn'nnn_
Cuts at: 1353 2624 2629 3904 1353
Size: 1271 5 1275 3423
Fragments arranged by size:
3423 1275 1271 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2456 2839 4561 4935 5081 387
Size: 1064 138 867 383 1722 374 146 1280
Fragments arranged by size:
1722 1280 1064 867 383 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 4037 4634 5421 5689 1239
Size: 393 2405 597 787 268 1524
Fragments arranged by size:
2405 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2517 3237 2517
Size: 720 5254
EcoRI G'AATT_C
Cuts at: 2995 3283 3661 2995
Size: 288 378 5308
Fragments arranged by size:
5308 378 288
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2103 2479 2103
Size: 376 5598
FspI TGC'GCA
Cuts at: 4480 4480
Size: 5974
HaeII r_GCGC'y
Cuts at: 1678 2957 5121 1678
Size: 1279 2164 2531
Fragments arranged by size:
2531 2164 1279
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2003 2035 2144 2176 2628
Size: 32 135 32 431 32 109 32 452
Cuts at: 2628 2660 2720 2752 4615 4647 4689 4721 1373
Size: 32 60 32 1863 32 42 32 2626
Fragments arranged by size:
2626 1863 452 431 135 109 60 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3318 3564 669
Size: 988 281 1380 246 3079
Fragments arranged by size:
3079 1380 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2226 748
Size: 1478 4496
HpaI GTT'AAC
Cuts at: 3564 3564
Size: 5974
KpnI G_GTAC'C
Cuts at: 3107 3299 3107
Size: 192 5782
MfeI C'AATT_G
Cuts at: 3573 3573
Size: 5974
MluI A'CGCG_T
Cuts at: 5850 5850
Size: 5974
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2233 5088 5272 1305
Size: 928 2855 184 2007
Fragments arranged by size:
2855 2007 928 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4152
Fragments arranged by size:
4152 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2927 2990 3155 3932 4291 4450 518
Size: 648 1761 63 165 777 359 159 2042
Fragments arranged by size:
2042 1761 777 648 359 165 159 63
MspA1I CmG'CkG
Cuts at: 1606 2936 3390 4067 5215 5460 1606
Size: 1330 454 677 1148 245 2120
Fragments arranged by size:
2120 1330 1148 677 454 245
NaeI GCC'GGC
Cuts at: 2886 2890 2886
Size: 4 5970
NcoI C'CATG_G
Cuts at: 513 1065 2004 3048 3150 513
Size: 552 939 1044 102 3337
Fragments arranged by size:
3337 1044 939 552 102
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5974
NgoMIV G'CCGG_C
Cuts at: 2884 2888 2884
Size: 4 5970
NheI G'CTAG_C
Cuts at: 1052 3227 1052
Size: 2175 3799
NotI GC'GGCC_GC
Cuts at: 3421 3421
Size: 5974
NspI r_CATG'y
Cuts at: 1132 1229 3082 3223 3332 4877 1132
Size: 97 1853 141 109 1545 2229
Fragments arranged by size:
2229 1853 1545 141 109 97
PasI CC'CwG_GG
Cuts at: 1348 2269 2942 1348
Size: 921 673 4380
Fragments arranged by size:
4380 921 673
PciI A'CATG_T
Cuts at: 4873 4873
Size: 5974
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2215 2352 1108
Size: 1107 137 4730
Fragments arranged by size:
4730 1107 137
PmeI GTTT'AAAC
Cuts at: 3278 3278
Size: 5974
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4043 4075 5556 5588 4043
Size: 32 1481 32 4429
Fragments arranged by size:
4429 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2517 2517
Size: 5974
PshAI GACnn'nnGTC
Cuts at: 1613 2868 1613
Size: 1255 4719
PsiI TTA'TAA
Cuts at: 3544 3544
Size: 5974
PspOMI G'GGCC_C
Cuts at: 3236 3236
Size: 5974
PstI C_TGCA'G
Cuts at: 830 2953 3326 830
Size: 2123 373 3478
Fragments arranged by size:
3478 2123 373
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5974
SalI G'TCGA_C
Cuts at: 1936 3316 1936
Size: 1380 4594
SbfI CC_TGCA'GG
Cuts at: 3326 3326
Size: 5974
ScaI AGT'ACT
Cuts at: 4222 5934 4222
Size: 1712 4262
SexAI A'CCwGG_T
Cuts at: 2754 2754
Size: 5974
SfcI C'TryA_G
Cuts at: 826 1045 2949 3322 4457 5138 5329 826
Size: 219 1904 373 1135 681 191 1471
Fragments arranged by size:
1904 1471 1135 681 373 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5974
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5974
SmaI CCC'GGG
Cuts at: 1872 2157 3301 1872
Size: 285 1144 4545
Fragments arranged by size:
4545 1144 285
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2179 4078 4979 5241 820
Size: 197 926 236 1899 901 262 1553
Fragments arranged by size:
1899 1553 926 901 262 236 197
SnaBI TAC'GTA
Cuts at: 493 3765 493
Size: 3272 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5974
SphI G_CATG'C
Cuts at: 1132 3082 3332 1132
Size: 1950 250 3774
Fragments arranged by size:
3774 1950 250
SspI AAT'ATT
Cuts at: 5 52 3898 5
Size: 47 3846 2081
Fragments arranged by size:
3846 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5974
StyI C'CwwG_G
Cuts at: 513 1065 2004 3048 3150 3413 513
Size: 552 939 1044 102 263 3074
Fragments arranged by size:
3074 1044 939 552 263 102
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2139 2630 2810 4001 4018 4171 4356
Size: 1255 559 491 180 1191 17 153 185
Cuts at: 4356 4801 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1191 559 491 445 185 180
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3216 4220 5932
Size: 274 80 33 51 1443 1239 1004 1712
Cuts at: 5932 96
Size: 138
Fragments arranged by size:
1712 1443 1239 1004 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3049 3081 5934 5966 3049
Size: 32 2853 32 3057
Fragments arranged by size:
3057 2853 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2756 1316
Size: 1440 4534
XbaI T'CTAG_A
Cuts at: 3310 3310
Size: 5974
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2310 2804 2310
Size: 494 5480
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5974
XmaI C'CCGG_G
Cuts at: 1870 2155 3299 1870
Size: 285 1144 4545
Fragments arranged by size:
4545 1144 285
XmnI GAAnn'nnTTC
Cuts at: 4103 4103
Size: 5974
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2260 2422 3782 276
Size: 53 83 186 936 726 162 1360 2468
Fragments arranged by size:
2468 1360 936 726 186 162 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I
BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI
FspAI KasI NarI NruI NsiI PacI PfoI PmlI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SfiI SfoI SrfI SwaI