Restriction map for
pFN21AB8315
(Circular) (Six-base) MAPSORT of: pFN21AB8315.seq Check: 1702 from: 1 to: 5467
pFN21AB8315 5467 bp
With 182 enzymes: SgfI *
February 27, 2009 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3277 278
Size: 53 83 186 936 1741 2468
Fragments arranged by size:
2468 1741 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2810 1937
Size: 873 4594
Acc65I G'GTAC_C
Cuts at: 2788 2788
Size: 5467
AclI AA'CG_TT
Cuts at: 1563 3594 3967 1563
Size: 2031 373 3063
Fragments arranged by size:
3063 2031 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2454 2769 3530 4914 5182 1632
Size: 822 315 761 1384 268 1917
Fragments arranged by size:
1917 1384 822 761 315 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5270
AflIII A'CryG_T
Cuts at: 1203 4366 5343 1203
Size: 3163 977 1327
Fragments arranged by size:
3163 1327 977
AgeI A'CCGG_T
Cuts at: 4298 4298
Size: 5467
AhdI GACnn_n'nnGTC
Cuts at: 4196 4196
Size: 5467
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5467
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5467
AlwNI CAG_nnn'CTG
Cuts at: 2384 2692 3210 4782 2384
Size: 308 518 1572 3069
Fragments arranged by size:
3069 1572 518 308
ApaLI G'TGCA_C
Cuts at: 3524 4680 3524
Size: 1156 4311
ApoI r'AATT_y
Cuts at: 1493 2197 2776 3002 3154 5263 1493
Size: 704 579 226 152 2109 1697
Fragments arranged by size:
2109 1697 704 579 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4021 160
Size: 872 2989 1606
Fragments arranged by size:
2989 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5467
AvaI C'yCGr_G
Cuts at: 1870 1943 2327 2792 1870
Size: 73 384 465 4545
Fragments arranged by size:
4545 465 384 73
BamHI G'GATC_C
Cuts at: 2797 3164 2797
Size: 367 5100
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2788 4243 618
Size: 325 217 627 1001 1455 1842
Fragments arranged by size:
1842 1455 1001 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2328 2531 2786 1391
Size: 937 203 255 4072
Fragments arranged by size:
4072 937 255 203
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2418 928
Size: 971 519 3977
Fragments arranged by size:
3977 971 519
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2007 2041 2746 2780 3658 3692 1903
Size: 34 70 34 705 34 878 34 3678
Fragments arranged by size:
3678 878 705 70 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2023 3359 4569 2023
Size: 1336 1210 2921
Fragments arranged by size:
2921 1336 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5467
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4078 136
Size: 107 122 71 631 605 2406 1525
Fragments arranged by size:
2406 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2020 2115 5462 2020
Size: 95 3347 2025
Fragments arranged by size:
3347 2025 95
BlpI GC'TnA_GC
Cuts at: 2884 2884
Size: 5467
Bme1580I G_kGCm'C
Cuts at: 1790 3528 4684 1790
Size: 1738 1156 2573
Fragments arranged by size:
2573 1738 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5467
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3198 4156 442
Size: 416 53 83 399 24 1781 958 1753
Fragments arranged by size:
1781 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5467
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2506 4127 1239
Size: 1267 1621 2579
Fragments arranged by size:
2579 1621 1267
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3592 4457 4755 3592
Size: 865 298 4304
Fragments arranged by size:
4304 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2123 4130 882
Size: 617 624 2007 2219
Fragments arranged by size:
2219 2007 624 617
BsaAI yAC'GTr
Cuts at: 493 1204 3258 493
Size: 711 2054 2702
Fragments arranged by size:
2702 2054 711
BsaBI GATnn'nnATC
Cuts at: 2415 5461 2415
Size: 3046 2421
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3274 3656 4291 275
Size: 53 83 186 936 1741 382 635 1451
Fragments arranged by size:
1741 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3900 4298 4572 4719 5130 3900
Size: 398 274 147 411 4237
Fragments arranged by size:
4237 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2315 2345 1178
Size: 30 165 30 912 30 4300
Fragments arranged by size:
4300 912 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2164 2453 2551 2572 1186
Size: 978 289 98 21 4081
Fragments arranged by size:
4081 978 289 98 21
BseYI C'CCAG_C
Cuts at: 2156 2648 2653 4670 5166 2156
Size: 492 5 2017 496 2457
Fragments arranged by size:
2457 2017 496 492 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4894
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2473 2917 3678 4706 1540
Size: 275 186 472 444 761 1028 2301
Fragments arranged by size:
2301 1028 761 472 444 275 186
BsiHKAI G_wGCw'C
Cuts at: 2531 2641 3528 3613 4684 2531
Size: 110 887 85 1071 3314
Fragments arranged by size:
3314 1071 887 110 85
BsmI GAATG_Cn'
Cuts at: 2978 3071 2978
Size: 93 5374
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2328 2531 2641 2786 3528 3613 4684
Size: 399 538 203 110 145 742 85 1071
Cuts at: 4684 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 538 399 203 145 110
85
BspEI T'CCGG_A
Cuts at: 5130 5130
Size: 5467
BspHI T'CATG_A
Cuts at: 3356 5086 3356
Size: 1730 3737
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2822 844
Size: 1978 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2677 3354 1394
Size: 429 854 677 3507
Fragments arranged by size:
3507 854 677 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3962 4136 65
Size: 1183 2714 174 1396
Fragments arranged by size:
2714 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2055 2336 4111 4298 1700
Size: 110 245 281 1775 187 2869
Fragments arranged by size:
2869 1775 281 245 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5467
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5467
BssSI C'ACGA_G
Cuts at: 3527 4539 3527
Size: 1012 4455
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5467
BstBI TT'CG_AA
Cuts at: 4271 4271
Size: 5467
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5467
BstYI r'GATC_y
Cuts at: 1972 2020 2115 2797 3164 3549 3566 5018 5104
Size: 48 95 682 367 385 17 1452 86
Cuts at: 5104 5116 5462 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 682 385 367 346 95 86
48 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3976
Fragments arranged by size:
3976 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4728
Fragments arranged by size:
4728 683 56
BtsI GCAGTG_nn'
Cuts at: 2983 3777 3797 2983
Size: 794 20 4653
Fragments arranged by size:
4653 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5467
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5432
DraI TTT'AAA
Cuts at: 2771 2771
Size: 5467
DraIII CAC_nnn'GTG
Cuts at: 2404 2404
Size: 5467
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4474 809
Size: 507 3158 1802
Fragments arranged by size:
3158 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2470 2914 3803
Size: 54 1425 50 275 18 640 444 889
Cuts at: 3803 5432 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 640 444 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2470 2914 1537
Size: 275 658 444 4090
Fragments arranged by size:
4090 658 444 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2286 2430 2602 2726 3397 1353
Size: 933 144 172 124 671 3423
Fragments arranged by size:
3423 933 671 172 144 124
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2155 2345 4054 4428 4574 387
Size: 1064 138 566 190 1709 374 146 1280
Fragments arranged by size:
1709 1280 1064 566 374 190 146 138
EcoICRI GAG'CTC
Cuts at: 2529 2529
Size: 5467
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2454 2506 2769 3530 4127 4914 5182
Size: 393 822 52 263 761 597 787 268
Cuts at: 5182 1239
Size: 1524
Fragments arranged by size:
1524 822 787 761 597 393 268 263
52
EcoNI CCTnn'n_nnAGG
Cuts at: 2082 2082
Size: 5467
EcoO109I rG'GnC_Cy
Cuts at: 2671 2671
Size: 5467
EcoRI G'AATT_C
Cuts at: 2197 2776 3154 2197
Size: 579 378 4510
Fragments arranged by size:
4510 579 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2134 2134
Size: 5467
FspI TGC'GCA
Cuts at: 3973 3973
Size: 5467
HaeII r_GCGC'y
Cuts at: 1678 4614 1678
Size: 2936 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2315 2347 2408 2440 4108
Size: 32 135 32 743 32 61 32 1668
Cuts at: 4108 4140 4182 4214 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1668 743 135 61 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2811 3057 669
Size: 988 281 873 246 3079
Fragments arranged by size:
3079 988 873 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5467
HpaI GTT'AAC
Cuts at: 3057 3057
Size: 5467
KpnI G_GTAC'C
Cuts at: 2792 2792
Size: 5467
MfeI C'AATT_G
Cuts at: 3066 3066
Size: 5467
MluI A'CGCG_T
Cuts at: 5343 5343
Size: 5467
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4581 4765 1305
Size: 3276 184 2007
Fragments arranged by size:
3276 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3645
Fragments arranged by size:
3645 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3425 3784 3943 518
Size: 648 2259 359 159 2042
Fragments arranged by size:
2259 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2689 2883 3560 4708 4953 1606
Size: 1083 194 677 1148 245 2120
Fragments arranged by size:
2120 1148 1083 677 245 194
NaeI GCC'GGC
Cuts at: 2338 2338
Size: 5467
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3976
Fragments arranged by size:
3976 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5467
NgoMIV G'CCGG_C
Cuts at: 2336 2336
Size: 5467
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5467
NotI GC'GGCC_GC
Cuts at: 2914 2914
Size: 5467
NspI r_CATG'y
Cuts at: 1132 1229 2825 4370 1132
Size: 97 1596 1545 2229
Fragments arranged by size:
2229 1596 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5467
PciI A'CATG_T
Cuts at: 4366 4366
Size: 5467
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5467
PfoI T'CCnGG_A
Cuts at: 2522 2522
Size: 5467
PmeI GTTT'AAAC
Cuts at: 2771 2771
Size: 5467
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3536 3568 5049 5081 3536
Size: 32 1481 32 3922
Fragments arranged by size:
3922 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2671 2671
Size: 5467
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5467
PsiI TTA'TAA
Cuts at: 3037 3037
Size: 5467
PstI C_TGCA'G
Cuts at: 830 2819 830
Size: 1989 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5467
PvuII CAG'CTG
Cuts at: 2689 2689
Size: 5467
SacI G_AGCT'C
Cuts at: 2531 2531
Size: 5467
SalI G'TCGA_C
Cuts at: 1936 2809 1936
Size: 873 4594
SbfI CC_TGCA'GG
Cuts at: 2819 2819
Size: 5467
ScaI AGT'ACT
Cuts at: 3715 5427 3715
Size: 1712 3755
SexAI A'CCwGG_T
Cuts at: 2400 2400
Size: 5467
SfcI C'TryA_G
Cuts at: 826 1045 2079 2815 3950 4631 4822 826
Size: 219 1034 736 1135 681 191 1471
Fragments arranged by size:
1471 1135 1034 736 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5467
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5467
SmaI CCC'GGG
Cuts at: 1872 2794 1872
Size: 922 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3571 4472 4734 820
Size: 197 926 1628 901 262 1553
Fragments arranged by size:
1628 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3258 493
Size: 2765 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5467
SphI G_CATG'C
Cuts at: 1132 2825 1132
Size: 1693 3774
SspI AAT'ATT
Cuts at: 5 52 3391 5
Size: 47 3339 2081
Fragments arranged by size:
3339 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5467
StyI C'CwwG_G
Cuts at: 513 1065 2004 2906 513
Size: 552 939 902 3074
Fragments arranged by size:
3074 939 902 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3494 3511 3664 3849 4294 325
Size: 1255 1914 17 153 185 445 1498
Fragments arranged by size:
1914 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3713 5425 96
Size: 274 80 33 51 1443 1736 1712 138
Fragments arranged by size:
1736 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5427 5459 5427
Size: 32 5435
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5467
XbaI T'CTAG_A
Cuts at: 2803 2803
Size: 5467
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2530 2530
Size: 5467
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5467
XmaI C'CCGG_G
Cuts at: 1870 2792 1870
Size: 922 4545
XmnI GAAnn'nnTTC
Cuts at: 2117 3596 2117
Size: 1479 3988
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3275 276
Size: 53 83 186 936 1741 2468
Fragments arranged by size:
2468 1741 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I EcoRV FseI FspAI KasI NarI NruI NsiI
PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI
SapI SfiI SfoI SrfI SwaI