Restriction map for
pFN21AB8313
(Circular) (Six-base) MAPSORT of: pFN21AB8313.seq Check: 7030 from: 1 to: 5809
pFN21AB8313 5809 bp
With 182 enzymes: SgfI *
February 27, 2009 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2562 2562
Size: 5809
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3619 278
Size: 53 83 186 936 2083 2468
Fragments arranged by size:
2468 2083 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2859 3152 1937
Size: 922 293 4594
Fragments arranged by size:
4594 922 293
Acc65I G'GTAC_C
Cuts at: 3130 3130
Size: 5809
AclI AA'CG_TT
Cuts at: 1563 3936 4309 1563
Size: 2373 373 3063
Fragments arranged by size:
3063 2373 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2159 3872 5256 5524 1632
Size: 527 1713 1384 268 1917
Fragments arranged by size:
1917 1713 1384 527 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5612
AflIII A'CryG_T
Cuts at: 1203 4708 5685 1203
Size: 3505 977 1327
Fragments arranged by size:
3505 1327 977
AgeI A'CCGG_T
Cuts at: 4640 4640
Size: 5809
AhdI GACnn_n'nnGTC
Cuts at: 4538 4538
Size: 5809
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5809
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2724 2781 1184
Size: 1540 57 4212
Fragments arranged by size:
4212 1540 57
AlwNI CAG_nnn'CTG
Cuts at: 2203 2806 2900 2947 2990 3552 5124 2203
Size: 603 94 47 43 562 1572 2888
Fragments arranged by size:
2888 1572 603 562 94 47 43
ApaLI G'TGCA_C
Cuts at: 3866 5022 3866
Size: 1156 4653
ApoI r'AATT_y
Cuts at: 1493 3118 3344 3496 5605 1493
Size: 1625 226 152 2109 1697
Fragments arranged by size:
2109 1697 1625 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4363 160
Size: 872 3331 1606
Fragments arranged by size:
3331 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5809
AvaI C'yCGr_G
Cuts at: 1870 1943 2162 2256 2476 3134 1870
Size: 73 219 94 220 658 4545
Fragments arranged by size:
4545 658 220 219 94 73
BamHI G'GATC_C
Cuts at: 3139 3506 3139
Size: 367 5442
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3130 4585 618
Size: 325 217 627 1343 1455 1842
Fragments arranged by size:
1842 1455 1343 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2382 2538 3128 1391
Size: 991 156 590 4072
Fragments arranged by size:
4072 991 590 156
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4838
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4000 4034 1903
Size: 34 2063 34 3678
Fragments arranged by size:
3678 2063 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3701 4911 3701
Size: 1210 4599
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5809
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2067 4420 136
Size: 107 122 71 631 605 395 2353 1525
Fragments arranged by size:
2353 1525 631 605 395 122 107 71
BglII A'GATC_T
Cuts at: 3042 5804 3042
Size: 2762 3047
BlpI GC'TnA_GC
Cuts at: 3226 3226
Size: 5809
Bme1580I G_kGCm'C
Cuts at: 1790 3870 5026 1790
Size: 2080 1156 2573
Fragments arranged by size:
2573 2080 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5809
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3540 4498 442
Size: 416 53 83 399 24 2123 958 1753
Fragments arranged by size:
2123 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5809
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2557 2557
Size: 5809
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2452 4469 1239
Size: 1213 2017 2579
Fragments arranged by size:
2579 2017 1213
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2369 3934 4799 5097 2369
Size: 1565 865 298 3081
Fragments arranged by size:
3081 1565 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3008 4472 882
Size: 617 1509 1464 2219
Fragments arranged by size:
2219 1509 1464 617
BsaAI yAC'GTr
Cuts at: 493 1204 3600 493
Size: 711 2396 2702
Fragments arranged by size:
2702 2396 711
BsaBI GATnn'nnATC
Cuts at: 5803 5803
Size: 5809
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3616 3998 4633 275
Size: 53 83 186 936 2083 382 635 1451
Fragments arranged by size:
2083 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2445 4242 4640 4914 5061 5472 2445
Size: 1797 398 274 147 411 2782
Fragments arranged by size:
2782 1797 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5584
Fragments arranged by size:
5584 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2372 2600 2678 2681 2684 1186
Size: 1186 228 78 3 3 4311
Fragments arranged by size:
4311 1186 228 78 3 3
BseYI C'CCAG_C
Cuts at: 2338 2527 2872 3048 5012 5508 2338
Size: 189 345 176 1964 496 2639
Fragments arranged by size:
2639 1964 496 345 189 176
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2230 1163
Size: 573 494 4742
Fragments arranged by size:
4742 573 494
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2062 3259 4020 5048 1540
Size: 275 186 61 1197 761 1028 2301
Fragments arranged by size:
2301 1197 1028 761 275 186 61
BsiHKAI G_wGCw'C
Cuts at: 2382 2468 2493 2538 3870 3955 5026 2382
Size: 86 25 45 1332 85 1071 3165
Fragments arranged by size:
3165 1332 1071 86 85 45 25
BsmI GAATG_Cn'
Cuts at: 3320 3413 3320
Size: 93 5716
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2382 2468 2493 2538 3128 3870 3955
Size: 399 592 86 25 45 590 742 85
Cuts at: 3955 5026 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 592 590 399 86 85
45 25
BspEI T'CCGG_A
Cuts at: 2445 5472 2445
Size: 3027 2782
BspHI T'CATG_A
Cuts at: 3069 3698 5428 3069
Size: 629 1730 3450
Fragments arranged by size:
3450 1730 629
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2562 3164 844
Size: 1718 602 3489
Fragments arranged by size:
3489 1718 602
BsrBI CCG'CTC
Cuts at: 1394 1823 3696 1394
Size: 429 1873 3507
Fragments arranged by size:
3507 1873 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4304 4478 65
Size: 1183 3056 174 1396
Fragments arranged by size:
3056 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4453 4640 1700
Size: 110 2643 187 2869
Fragments arranged by size:
2869 2643 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5809
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5809
BssSI C'ACGA_G
Cuts at: 2345 3869 4881 2345
Size: 1524 1012 3273
Fragments arranged by size:
3273 1524 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5809
BstBI TT'CG_AA
Cuts at: 4613 4613
Size: 5809
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5809
BstYI r'GATC_y
Cuts at: 1972 2235 2319 3042 3139 3506 3891 3908 5360
Size: 263 84 723 97 367 385 17 1452
Cuts at: 5360 5446 5458 5804 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 723 385 367 346 263 97
86 84 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2062 2585 3009 513
Size: 552 939 58 523 424 3313
Fragments arranged by size:
3313 939 552 523 424 58
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5070
Fragments arranged by size:
5070 683 56
BtsI GCAGTG_nn'
Cuts at: 2270 2642 3325 4119 4139 2270
Size: 372 683 794 20 3940
Fragments arranged by size:
3940 794 683 372 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5809
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5774
DraI TTT'AAA
Cuts at: 3113 3113
Size: 5809
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4816 809
Size: 507 3500 1802
Fragments arranged by size:
3500 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2059 3256 4145
Size: 54 1425 50 275 18 229 1197 889
Cuts at: 4145 5774 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1197 889 275 229 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2059 3256 1537
Size: 275 247 1197 4090
Fragments arranged by size:
4090 1197 275 247
EarI CTCTTCn'nnn_
Cuts at: 1353 2543 3739 1353
Size: 1190 1196 3423
Fragments arranged by size:
3423 1196 1190
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2696 4396 4770 4916 387
Size: 1064 138 1107 1700 374 146 1280
Fragments arranged by size:
1700 1280 1107 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2380 2536 2380
Size: 156 5653
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2159 2452 3872 4469 5256 5524 1239
Size: 393 527 293 1420 597 787 268 1524
Fragments arranged by size:
1524 1420 787 597 527 393 293 268
EcoO109I rG'GnC_Cy
Cuts at: 2143 2302 2897 2143
Size: 159 595 5055
Fragments arranged by size:
5055 595 159
EcoRI G'AATT_C
Cuts at: 3118 3496 3118
Size: 378 5431
FspI TGC'GCA
Cuts at: 4315 4315
Size: 5809
HaeII r_GCGC'y
Cuts at: 1678 2893 4956 1678
Size: 1215 2063 2531
Fragments arranged by size:
2531 2063 1215
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2063 2095 4450 4482 4524
Size: 32 135 32 491 32 2355 32 42
Cuts at: 4524 4556 1373
Size: 32 2626
Fragments arranged by size:
2626 2355 491 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3153 3399 669
Size: 988 281 1215 246 3079
Fragments arranged by size:
3079 1215 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5809
HpaI GTT'AAC
Cuts at: 3399 3399
Size: 5809
KpnI G_GTAC'C
Cuts at: 3134 3134
Size: 5809
MfeI C'AATT_G
Cuts at: 3408 3408
Size: 5809
MluI A'CGCG_T
Cuts at: 5685 5685
Size: 5809
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2847 4923 5107 1305
Size: 1542 2076 184 2007
Fragments arranged by size:
2076 2007 1542 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3987
Fragments arranged by size:
3987 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3767 4126 4285 518
Size: 648 2601 359 159 2042
Fragments arranged by size:
2601 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2698 3225 3902 5050 5295 1606
Size: 1092 527 677 1148 245 2120
Fragments arranged by size:
2120 1148 1092 677 527 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4318
Fragments arranged by size:
4318 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5809
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5809
NotI GC'GGCC_GC
Cuts at: 3256 3256
Size: 5809
NspI r_CATG'y
Cuts at: 1132 1229 2602 3167 4712 1132
Size: 97 1373 565 1545 2229
Fragments arranged by size:
2229 1545 1373 565 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5809
PciI A'CATG_T
Cuts at: 4708 4708
Size: 5809
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5809
PmeI GTTT'AAAC
Cuts at: 3113 3113
Size: 5809
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3878 3910 5391 5423 3878
Size: 32 1481 32 4264
Fragments arranged by size:
4264 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2143 2302 2143
Size: 159 5650
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5809
PsiI TTA'TAA
Cuts at: 3379 3379
Size: 5809
PspXI vC'TCGA_Gb
Cuts at: 2162 2476 2162
Size: 314 5495
PstI C_TGCA'G
Cuts at: 830 2277 2559 2703 3161 830
Size: 1447 282 144 458 3478
Fragments arranged by size:
3478 1447 458 282 144
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5809
PvuII CAG'CTG
Cuts at: 2698 2698
Size: 5809
SacI G_AGCT'C
Cuts at: 2382 2538 2382
Size: 156 5653
SalI G'TCGA_C
Cuts at: 1936 3151 1936
Size: 1215 4594
SanDI GG'GwC_CC
Cuts at: 2302 2302
Size: 5809
SapI GCTCTTCn'nnn_
Cuts at: 2543 2543
Size: 5809
SbfI CC_TGCA'GG
Cuts at: 3161 3161
Size: 5809
ScaI AGT'ACT
Cuts at: 2741 4057 5769 2741
Size: 1316 1712 2781
Fragments arranged by size:
2781 1712 1316
SfcI C'TryA_G
Cuts at: 826 1045 2273 2555 2699 2956 3157 4292 4973
Size: 219 1228 282 144 257 201 1135 681
Cuts at: 4973 5164 826
Size: 191 1471
Fragments arranged by size:
1471 1228 1135 681 282 257 219 201
191 144
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5809
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5809
SmaI CCC'GGG
Cuts at: 1872 2258 3136 1872
Size: 386 878 4545
Fragments arranged by size:
4545 878 386
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2162 2384 2476 3913 4814 5076
Size: 197 926 219 222 92 1437 901 262
Cuts at: 5076 820
Size: 1553
Fragments arranged by size:
1553 1437 926 901 262 222 219 197
92
SnaBI TAC'GTA
Cuts at: 493 3600 493
Size: 3107 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5809
SphI G_CATG'C
Cuts at: 1132 3167 1132
Size: 2035 3774
SspI AAT'ATT
Cuts at: 5 52 3733 5
Size: 47 3681 2081
Fragments arranged by size:
3681 2081 47
StuI AGG'CCT
Cuts at: 1517 2522 1517
Size: 1005 4804
StyI C'CwwG_G
Cuts at: 513 1065 2004 3248 513
Size: 552 939 1244 3074
Fragments arranged by size:
3074 1244 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2451 3836 3853 4006 4191 4636 325
Size: 1255 871 1385 17 153 185 445 1498
Fragments arranged by size:
1498 1385 1255 871 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2739 4055 5767
Size: 274 80 33 51 1443 762 1316 1712
Cuts at: 5767 96
Size: 138
Fragments arranged by size:
1712 1443 1316 762 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2192 2224 5769 5801 2192
Size: 32 3545 32 2200
Fragments arranged by size:
3545 2200 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5809
XbaI T'CTAG_A
Cuts at: 3145 3145
Size: 5809
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2116 2116
Size: 5809
XhoI C'TCGA_G
Cuts at: 1943 2162 2476 1943
Size: 219 314 5276
Fragments arranged by size:
5276 314 219
XmaI C'CCGG_G
Cuts at: 1870 2256 3134 1870
Size: 386 878 4545
Fragments arranged by size:
4545 878 386
XmnI GAAnn'nnTTC
Cuts at: 3938 3938
Size: 5809
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3617 276
Size: 53 83 186 936 2083 2468
Fragments arranged by size:
2468 2083 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PstI
PvuI PvuII SacI SalI SanDI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PsrI
RsrII SacII SexAI SfiI SfoI SrfI SwaI