Restriction map for
pFN21AB8310
(Circular) (Six-base) MAPSORT of: pFN21AB8310.seq Check: 2689 from: 1 to: 5914
pFN21AB8310 5914 bp
With 182 enzymes: SgfI *
February 27, 2009 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3724 278
Size: 53 83 186 936 2188 2468
Fragments arranged by size:
2468 2188 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2622 3257 1937
Size: 685 635 4594
Fragments arranged by size:
4594 685 635
Acc65I G'GTAC_C
Cuts at: 3235 3235
Size: 5914
AclI AA'CG_TT
Cuts at: 1563 4041 4414 1563
Size: 2478 373 3063
Fragments arranged by size:
3063 2478 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2654 3977 5361 5629 1632
Size: 1022 1323 1384 268 1917
Fragments arranged by size:
1917 1384 1323 1022 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5717
AflIII A'CryG_T
Cuts at: 1203 2074 4813 5790 1203
Size: 871 2739 977 1327
Fragments arranged by size:
2739 1327 977 871
AgeI A'CCGG_T
Cuts at: 4745 4745
Size: 5914
AhdI GACnn_n'nnGTC
Cuts at: 4643 4643
Size: 5914
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5914
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2718 1184
Size: 1534 4380
AlwNI CAG_nnn'CTG
Cuts at: 2039 2308 3657 5229 2039
Size: 269 1349 1572 2724
Fragments arranged by size:
2724 1572 1349 269
ApaLI G'TGCA_C
Cuts at: 3971 5127 3971
Size: 1156 4758
ApoI r'AATT_y
Cuts at: 1493 2158 2966 3038 3160 3223 3449 3601 5710
Size: 665 808 72 122 63 226 152 2109
Cuts at: 5710 1493
Size: 1697
Fragments arranged by size:
2109 1697 808 665 226 152 122 72
63
AseI AT'TA_AT
Cuts at: 160 1032 4468 160
Size: 872 3436 1606
Fragments arranged by size:
3436 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5914
AvaI C'yCGr_G
Cuts at: 1870 1943 3239 1870
Size: 73 1296 4545
Fragments arranged by size:
4545 1296 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2238 2271 2238
Size: 33 5881
BamHI G'GATC_C
Cuts at: 3244 3611 3244
Size: 367 5547
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2317 2876 3235 4690 618
Size: 325 217 627 530 559 359 1455 1842
Fragments arranged by size:
1842 1455 627 559 530 359 325 217
BanII G_rGCy'C
Cuts at: 1391 3233 1391
Size: 1842 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2403 928
Size: 971 504 4439
Fragments arranged by size:
4439 971 504
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4105 4139 1903
Size: 34 2168 34 3678
Fragments arranged by size:
3678 2168 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3806 5016 3806
Size: 1210 4704
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5914
BfrBI ATG'CAT
Cuts at: 2920 2920
Size: 5914
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4525 136
Size: 107 122 71 631 605 2853 1525
Fragments arranged by size:
2853 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5909 5909
Size: 5914
BlpI GC'TnA_GC
Cuts at: 2136 2505 3331 2136
Size: 369 826 4719
Fragments arranged by size:
4719 826 369
Bme1580I G_kGCm'C
Cuts at: 1790 3975 5131 1790
Size: 2185 1156 2573
Fragments arranged by size:
2573 2185 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5914
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3645 4603 442
Size: 416 53 83 399 24 2228 958 1753
Fragments arranged by size:
2228 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3204 1056
Size: 2148 3766
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2883 2883
Size: 5914
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2040 2185 2254 2531 2826 2962 4574 1239
Size: 801 145 69 277 295 136 1612 2579
Fragments arranged by size:
2579 1612 801 295 277 145 136 69
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4039 4904 5202 4039
Size: 865 298 4751
Fragments arranged by size:
4751 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3005 4577 882
Size: 617 1506 1572 2219
Fragments arranged by size:
2219 1572 1506 617
BsaAI yAC'GTr
Cuts at: 493 1204 3705 493
Size: 711 2501 2702
Fragments arranged by size:
2702 2501 711
BsaBI GATnn'nnATC
Cuts at: 5908 5908
Size: 5914
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3721 4103 4738 275
Size: 53 83 186 936 2188 382 635 1451
Fragments arranged by size:
2188 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4347 4745 5019 5166 5577 4347
Size: 398 274 147 411 4684
Fragments arranged by size:
4684 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5689
Fragments arranged by size:
5689 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2351 1186
Size: 1165 4749
BseYI C'CCAG_C
Cuts at: 2166 3107 5117 5613 2166
Size: 941 2010 496 2467
Fragments arranged by size:
2467 2010 941 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5341
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3364 4125 5153 1540
Size: 275 186 1363 761 1028 2301
Fragments arranged by size:
2301 1363 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3975 4060 5131 3975
Size: 85 1071 4758
Fragments arranged by size:
4758 1071 85
BsmI GAATG_Cn'
Cuts at: 3425 3518 3425
Size: 93 5821
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3233 3975 4060 5131 1391
Size: 399 1443 742 85 1071 2174
Fragments arranged by size:
2174 1443 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5577 5577
Size: 5914
BspHI T'CATG_A
Cuts at: 3803 5533 3803
Size: 1730 4184
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2898 3269 844
Size: 2054 371 3489
Fragments arranged by size:
3489 2054 371
BsrBI CCG'CTC
Cuts at: 1394 1823 3801 1394
Size: 429 1978 3507
Fragments arranged by size:
3507 1978 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4409 4583 65
Size: 1183 3161 174 1396
Fragments arranged by size:
3161 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2212 4558 4745 1700
Size: 110 402 2346 187 2869
Fragments arranged by size:
2869 2346 402 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5914
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5914
BssSI C'ACGA_G
Cuts at: 2081 3974 4986 2081
Size: 1893 1012 3009
Fragments arranged by size:
3009 1893 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2308 794
Size: 1514 4400
BstBI TT'CG_AA
Cuts at: 2750 4718 2750
Size: 1968 3946
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5914
BstYI r'GATC_y
Cuts at: 1972 2935 3244 3611 3996 4013 5465 5551 5563
Size: 963 309 367 385 17 1452 86 12
Cuts at: 5563 5909 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 963 385 367 346 309 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2334 2334
Size: 5914
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4423
Fragments arranged by size:
4423 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5175
Fragments arranged by size:
5175 683 56
BtsI GCAGTG_nn'
Cuts at: 2390 3430 4224 4244 2390
Size: 1040 794 20 4060
Fragments arranged by size:
4060 1040 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5914
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5879
DraI TTT'AAA
Cuts at: 2500 3218 2500
Size: 718 5196
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4921 809
Size: 507 3605 1802
Fragments arranged by size:
3605 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2128 3361 4250
Size: 54 1425 50 275 18 298 1233 889
Cuts at: 4250 5879 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1233 889 298 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3361 1537
Size: 275 1549 4090
Fragments arranged by size:
4090 1549 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2536 2747 3844 1353
Size: 1183 211 1097 3423
Fragments arranged by size:
3423 1183 1097 211
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4501 4875 5021 387
Size: 1064 138 2912 374 146 1280
Fragments arranged by size:
2912 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2040 2185 2254 2531 2654 2826 2962
Size: 393 408 145 69 277 123 172 136
Cuts at: 2962 3977 4574 5361 5629 1239
Size: 1015 597 787 268 1524
Fragments arranged by size:
1524 1015 787 597 408 393 277 268
172 145 136 123 69
EcoO109I rG'GnC_Cy
Cuts at: 2036 3076 2036
Size: 1040 4874
EcoRI G'AATT_C
Cuts at: 3038 3223 3601 3038
Size: 185 378 5351
Fragments arranged by size:
5351 378 185
FspI TGC'GCA
Cuts at: 4420 4420
Size: 5914
HaeII r_GCGC'y
Cuts at: 1678 5061 1678
Size: 3383 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2896 2928 4555 4587 4629
Size: 32 135 32 1324 32 1627 32 42
Cuts at: 4629 4661 1373
Size: 32 2626
Fragments arranged by size:
2626 1627 1324 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3258 3504 669
Size: 988 281 1320 246 3079
Fragments arranged by size:
3079 1320 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2192 748
Size: 1444 4470
HpaI GTT'AAC
Cuts at: 3504 3504
Size: 5914
KpnI G_GTAC'C
Cuts at: 3239 3239
Size: 5914
MfeI C'AATT_G
Cuts at: 2237 3513 2237
Size: 1276 4638
MluI A'CGCG_T
Cuts at: 5790 5790
Size: 5914
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2041 2894 5028 5212 1305
Size: 736 853 2134 184 2007
Fragments arranged by size:
2134 2007 853 736 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2130 10
Size: 54 1425 343 298 3794
Fragments arranged by size:
3794 1425 343 298 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3872 4231 4390 518
Size: 648 2706 359 159 2042
Fragments arranged by size:
2706 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2140 2509 2804 3330 4007 5155 5400 1606
Size: 534 369 295 526 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 534 526 369 295 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4423
Fragments arranged by size:
4423 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5914
NheI G'CTAG_C
Cuts at: 1052 3200 1052
Size: 2148 3766
NotI GC'GGCC_GC
Cuts at: 3361 3361
Size: 5914
NsiI A_TGCA'T
Cuts at: 2922 2922
Size: 5914
NspI r_CATG'y
Cuts at: 1132 1229 2846 3272 4817 1132
Size: 97 1617 426 1545 2229
Fragments arranged by size:
2229 1617 1545 426 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5914
PciI A'CATG_T
Cuts at: 4813 4813
Size: 5914
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5914
PfoI T'CCnGG_A
Cuts at: 2855 2855
Size: 5914
PmeI GTTT'AAAC
Cuts at: 3218 3218
Size: 5914
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3983 4015 5496 5528 3983
Size: 32 1481 32 4369
Fragments arranged by size:
4369 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2036 3076 2036
Size: 1040 4874
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5914
PsiI TTA'TAA
Cuts at: 3484 3484
Size: 5914
PstI C_TGCA'G
Cuts at: 830 3266 830
Size: 2436 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5914
PvuII CAG'CTG
Cuts at: 2509 2804 2509
Size: 295 5619
SalI G'TCGA_C
Cuts at: 1936 3256 1936
Size: 1320 4594
SbfI CC_TGCA'GG
Cuts at: 3266 3266
Size: 5914
ScaI AGT'ACT
Cuts at: 3135 4162 5874 3135
Size: 1027 1712 3175
Fragments arranged by size:
3175 1712 1027
SfcI C'TryA_G
Cuts at: 826 1045 2049 3262 4397 5078 5269 826
Size: 219 1004 1213 1135 681 191 1471
Fragments arranged by size:
1471 1213 1135 1004 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5914
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5914
SmaI CCC'GGG
Cuts at: 1872 3241 1872
Size: 1369 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4018 4919 5181 820
Size: 197 926 2075 901 262 1553
Fragments arranged by size:
2075 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3705 493
Size: 3212 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5914
SphI G_CATG'C
Cuts at: 1132 3272 1132
Size: 2140 3774
SspI AAT'ATT
Cuts at: 5 52 2581 3838 5
Size: 47 2529 1257 2081
Fragments arranged by size:
2529 2081 1257 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5914
StyI C'CwwG_G
Cuts at: 513 1065 2004 2947 3353 513
Size: 552 939 943 406 3074
Fragments arranged by size:
3074 943 939 552 406
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2025 2303 2820 3941 3958 4111 4296
Size: 1255 445 278 517 1121 17 153 185
Cuts at: 4296 4741 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1121 517 445 445 278 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2839 3133 4160
Size: 274 80 33 51 1443 862 294 1027
Cuts at: 4160 5872 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1027 862 294 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5874 5906 5874
Size: 32 5882
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5914
XbaI T'CTAG_A
Cuts at: 3250 3250
Size: 5914
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5914
XmaI C'CCGG_G
Cuts at: 1870 3239 1870
Size: 1369 4545
XmnI GAAnn'nnTTC
Cuts at: 4043 4043
Size: 5914
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3722 276
Size: 53 83 186 936 2188 2468
Fragments arranged by size:
2468 2188 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI