Restriction map for
pFN21AB8305
(Circular) (Six-base) MAPSORT of: pFN21AB8305.seq Check: 9608 from: 1 to: 6676
pFN21AB8305 6676 bp
With 182 enzymes: SgfI *
October 20, 2009 10:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2071 2232 4486 278
Size: 53 83 186 936 535 161 2254 2468
Fragments arranged by size:
2468 2254 936 535 186 161 83 53
AccI GT'mk_AC
Cuts at: 1937 4019 1937
Size: 2082 4594
Acc65I G'GTAC_C
Cuts at: 3997 3997
Size: 6676
AclI AA'CG_TT
Cuts at: 1563 4803 5176 1563
Size: 3240 373 3063
Fragments arranged by size:
3240 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3915 4739 6123 6391 1632
Size: 2283 824 1384 268 1917
Fragments arranged by size:
2283 1917 1384 824 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6479
AflIII A'CryG_T
Cuts at: 1203 2937 3744 5575 6552 1203
Size: 1734 807 1831 977 1327
Fragments arranged by size:
1831 1734 1327 977 807
AgeI A'CCGG_T
Cuts at: 5507 5507
Size: 6676
AhdI GACnn_n'nnGTC
Cuts at: 5405 5405
Size: 6676
AleI CACnn'nnGTG
Cuts at: 1166 3020 3767 1166
Size: 1854 747 4075
Fragments arranged by size:
4075 1854 747
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2679 3653 1184
Size: 1495 974 4207
Fragments arranged by size:
4207 1495 974
AlwNI CAG_nnn'CTG
Cuts at: 3517 4419 5991 3517
Size: 902 1572 4202
Fragments arranged by size:
4202 1572 902
ApaLI G'TGCA_C
Cuts at: 2945 4733 5889 2945
Size: 1788 1156 3732
Fragments arranged by size:
3732 1788 1156
ApoI r'AATT_y
Cuts at: 1493 2216 2761 3985 4211 4363 6472 1493
Size: 723 545 1224 226 152 2109 1697
Fragments arranged by size:
2109 1697 1224 723 545 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5230 160
Size: 872 4198 1606
Fragments arranged by size:
4198 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6676
AvaI C'yCGr_G
Cuts at: 1870 1943 2570 4001 1870
Size: 73 627 1431 4545
Fragments arranged by size:
4545 1431 627 73
BamHI G'GATC_C
Cuts at: 2740 4006 4373 2740
Size: 1266 367 5043
Fragments arranged by size:
5043 1266 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2586 3022 3096 3183 3209
Size: 325 217 627 799 436 74 87 26
Cuts at: 3209 3997 5452 618
Size: 788 1455 1842
Fragments arranged by size:
1842 1455 799 788 627 436 325 217
87 74 26
BanII G_rGCy'C
Cuts at: 1391 2367 2577 3446 3704 3995 1391
Size: 976 210 869 258 291 4072
Fragments arranged by size:
4072 976 869 291 258 210
BbeI G_GCGC'C
Cuts at: 3100 3187 3100
Size: 87 6589
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3413 928
Size: 971 1514 4191
Fragments arranged by size:
4191 1514 971
BbvCI CC'TCA_GC
Cuts at: 3855 3936 3855
Size: 81 6595
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4867 4901 1903
Size: 34 2930 34 3678
Fragments arranged by size:
3678 2930 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4568 5778 4568
Size: 1210 5466
BclI T'GATC_A
Cuts at: 1548 3711 1548
Size: 2163 4513
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5287 136
Size: 107 122 71 631 605 3615 1525
Fragments arranged by size:
3615 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6671 6671
Size: 6676
BlpI GC'TnA_GC
Cuts at: 2152 4093 2152
Size: 1941 4735
Bme1580I G_kGCm'C
Cuts at: 1790 2842 2949 3027 3172 4737 5893 1790
Size: 1052 107 78 145 1565 1156 2573
Fragments arranged by size:
2573 1565 1156 1052 145 107 78
BmgBI CAC'GTC
Cuts at: 1318 3241 3254 3745 1318
Size: 1923 13 491 4249
Fragments arranged by size:
4249 1923 491 13
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2527 3159 4407
Size: 416 53 83 399 24 1110 632 1248
Cuts at: 4407 5365 442
Size: 958 1753
Fragments arranged by size:
1753 1248 1110 958 632 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6676
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2039 3013 3344 3539 5336 1239
Size: 800 974 331 195 1797 2579
Fragments arranged by size:
2579 1797 974 800 331 195
Bpu10I CC'TnA_GC
Cuts at: 2646 2747 3041 3855 3936 2646
Size: 101 294 814 81 5386
Fragments arranged by size:
5386 814 294 101 81
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2413 4801 5666 5964 2413
Size: 2388 865 298 3125
Fragments arranged by size:
3125 2388 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2084 2711 3919 5339 882
Size: 617 585 627 1208 1420 2219
Fragments arranged by size:
2219 1420 1208 627 617 585
BsaAI yAC'GTr
Cuts at: 493 1204 2938 4467 493
Size: 711 1734 1529 2702
Fragments arranged by size:
2702 1734 1529 711
BsaBI GATnn'nnATC
Cuts at: 6670 6670
Size: 6676
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2068 2229 2995 3097
Size: 53 83 186 936 535 161 766 102
Cuts at: 3097 3184 4483 4865 5500 275
Size: 87 1299 382 635 1451
Fragments arranged by size:
1451 1299 936 766 635 535 382 186
161 102 87 83 53
BsaWI w'CCGG_w
Cuts at: 2073 2817 5109 5507 5781 5928 6339 2073
Size: 744 2292 398 274 147 411 2410
Fragments arranged by size:
2410 2292 744 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3145 3175 3512 3542 1178
Size: 30 165 30 1742 30 337 30 4312
Fragments arranged by size:
4312 1742 337 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3953 1186
Size: 2767 3909
BseYI C'CCAG_C
Cuts at: 3596 3610 3840 5879 6375 3596
Size: 14 230 2039 496 3897
Fragments arranged by size:
3897 2039 496 230 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2214 2775 3155 3392 3521 3745 1163
Size: 573 478 561 380 237 129 224 4094
Fragments arranged by size:
4094 573 561 478 380 237 224 129
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4126 4887 5915 1540
Size: 275 186 2125 761 1028 2301
Fragments arranged by size:
2301 2125 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2250 2310 2367 2949 3047 3322 3478 4737 4822
Size: 60 57 582 98 275 156 1259 85
Cuts at: 4822 5893 2250
Size: 1071 3033
Fragments arranged by size:
3033 1259 1071 582 275 156 98 85
60 57
BsmI GAATG_Cn'
Cuts at: 3127 4187 4280 3127
Size: 1060 93 5523
Fragments arranged by size:
5523 1060 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2250 2310 2367 2577 2842 2949 3027
Size: 399 460 60 57 210 265 107 78
Cuts at: 3027 3047 3172 3322 3446 3478 3704 3995 4737
Size: 20 125 150 124 32 226 291 742
Cuts at: 4737 4822 5893 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 460 399 291 265 226
210 150 125 124 107 85 78 60
57 32 20
BspEI T'CCGG_A
Cuts at: 2817 6339 2817
Size: 3522 3154
BspHI T'CATG_A
Cuts at: 2034 4565 6295 2034
Size: 2531 1730 2415
Fragments arranged by size:
2531 2415 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3262 3543 4031 844
Size: 2418 281 488 3489
Fragments arranged by size:
3489 2418 488 281
BsrBI CCG'CTC
Cuts at: 1394 1823 2650 3036 4563 1394
Size: 429 827 386 1527 3507
Fragments arranged by size:
3507 1527 827 429 386
BsrDI GCAATG_nn'
Cuts at: 65 1248 2661 5171 5345 65
Size: 1183 1413 2510 174 1396
Fragments arranged by size:
2510 1413 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2280 5320 5507 1700
Size: 110 470 3040 187 2869
Fragments arranged by size:
3040 2869 470 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6676
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6676
BssSI C'ACGA_G
Cuts at: 4736 5748 4736
Size: 1012 5664
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2734 794
Size: 1940 4736
BstBI TT'CG_AA
Cuts at: 5480 5480
Size: 6676
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6676
BstXI CCAn_nnnn'nTGG
Cuts at: 2612 3424 2612
Size: 812 5864
BstYI r'GATC_y
Cuts at: 1972 2740 3333 3513 4006 4373 4758 4775 6227
Size: 768 593 180 493 367 385 17 1452
Cuts at: 6227 6313 6325 6671 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 768 593 493 385 367 346
180 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2444 3705 3896 2444
Size: 1261 191 5224
Fragments arranged by size:
5224 1261 191
BtgI C'CryG_G
Cuts at: 513 1065 2004 3091 3794 513
Size: 552 939 1087 703 3395
Fragments arranged by size:
3395 1087 939 703 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5937
Fragments arranged by size:
5937 683 56
BtsI GCAGTG_nn'
Cuts at: 2735 2758 4192 4986 5006 2735
Size: 23 1434 794 20 4405
Fragments arranged by size:
4405 1434 794 23 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6676
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6641
DraI TTT'AAA
Cuts at: 3980 3980
Size: 6676
DraIII CAC_nnn'GTG
Cuts at: 2314 2314
Size: 6676
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2903 5683 809
Size: 507 1587 2780 1802
Fragments arranged by size:
2780 1802 1587 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2614 3816 3883
Size: 54 1425 50 275 18 784 1202 67
Cuts at: 3883 4123 5012 6641 8
Size: 240 889 1629 43
Fragments arranged by size:
1629 1425 1202 889 784 275 240 67
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4123 1537
Size: 275 2311 4090
Fragments arranged by size:
4090 2311 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4606 1353
Size: 3253 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5263 5637 5783 387
Size: 1064 138 3674 374 146 1280
Fragments arranged by size:
3674 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2365 2365
Size: 6676
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2039 3013 3344 3539 3915 4739 5336
Size: 393 407 974 331 195 376 824 597
Cuts at: 5336 6123 6391 1239
Size: 787 268 1524
Fragments arranged by size:
1524 974 824 787 597 407 393 376
331 268 195
EcoNI CCTnn'n_nnAGG
Cuts at: 2791 3203 3217 2791
Size: 412 14 6250
Fragments arranged by size:
6250 412 14
EcoO109I rG'GnC_Cy
Cuts at: 2676 2676
Size: 6676
EcoRI G'AATT_C
Cuts at: 3985 4363 3985
Size: 378 6298
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2411 2411
Size: 6676
FspI TGC'GCA
Cuts at: 5182 5182
Size: 6676
HaeII r_GCGC'y
Cuts at: 1678 2932 3100 3187 5823 1678
Size: 1254 168 87 2636 2531
Fragments arranged by size:
2636 2531 1254 168 87
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2411 2443 2795 2827 3512
Size: 32 135 32 839 32 352 32 685
Cuts at: 3512 3544 3564 3596 5317 5349 5391 5423 1373
Size: 32 20 32 1721 32 42 32 2626
Fragments arranged by size:
2626 1721 839 685 352 135 42 32
32 32 32 32 32 32 32 20
HincII GTy'rAC
Cuts at: 669 1657 1938 4020 4266 669
Size: 988 281 2082 246 3079
Fragments arranged by size:
3079 2082 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6676
HpaI GTT'AAC
Cuts at: 4266 4266
Size: 6676
KasI G'GCGC_C
Cuts at: 3096 3183 3096
Size: 87 6589
KpnI G_GTAC'C
Cuts at: 4001 4001
Size: 6676
MfeI C'AATT_G
Cuts at: 2406 4275 2406
Size: 1869 4807
MluI A'CGCG_T
Cuts at: 6552 6552
Size: 6676
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5790 5974 1305
Size: 4485 184 2007
Fragments arranged by size:
4485 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4854
Fragments arranged by size:
4854 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3020 3422 3767 3908 4634 4993 5152
Size: 648 1854 402 345 141 726 359 159
Cuts at: 5152 518
Size: 2042
Fragments arranged by size:
2042 1854 726 648 402 359 345 159
141
MspA1I CmG'CkG
Cuts at: 1606 3717 3859 3935 4092 4769 5917 6162 1606
Size: 2111 142 76 157 677 1148 245 2120
Fragments arranged by size:
2120 2111 1148 677 245 157 142 76
NaeI GCC'GGC
Cuts at: 2282 2282
Size: 6676
NarI GG'CG_CC
Cuts at: 3097 3184 3097
Size: 87 6589
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5185
Fragments arranged by size:
5185 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6676
NgoMIV G'CCGG_C
Cuts at: 2280 2280
Size: 6676
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6676
NotI GC'GGCC_GC
Cuts at: 4123 4123
Size: 6676
NspI r_CATG'y
Cuts at: 1132 1229 2737 3436 3529 3670 4034 5579 1132
Size: 97 1508 699 93 141 364 1545 2229
Fragments arranged by size:
2229 1545 1508 699 364 141 97 93
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6676
PciI A'CATG_T
Cuts at: 5575 5575
Size: 6676
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2788 3180 1108
Size: 1680 392 4604
Fragments arranged by size:
4604 1680 392
PfoI T'CCnGG_A
Cuts at: 3516 3516
Size: 6676
PmeI GTTT'AAAC
Cuts at: 3980 3980
Size: 6676
PmlI CAC'GTG
Cuts at: 2938 2938
Size: 6676
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2690 2722 4745 4777 6258 6290 2690
Size: 32 2023 32 1481 32 3076
Fragments arranged by size:
3076 2023 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2676 2676
Size: 6676
PshAI GACnn'nnGTC
Cuts at: 1613 2112 1613
Size: 499 6177
PsiI TTA'TAA
Cuts at: 4246 4246
Size: 6676
PstI C_TGCA'G
Cuts at: 830 3642 4028 830
Size: 2812 386 3478
Fragments arranged by size:
3478 2812 386
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6676
PvuII CAG'CTG
Cuts at: 3859 3935 3859
Size: 76 6600
SacI G_AGCT'C
Cuts at: 2367 2367
Size: 6676
SalI G'TCGA_C
Cuts at: 1936 4018 1936
Size: 2082 4594
SbfI CC_TGCA'GG
Cuts at: 4028 4028
Size: 6676
ScaI AGT'ACT
Cuts at: 3377 4924 6636 3377
Size: 1547 1712 3417
Fragments arranged by size:
3417 1712 1547
SfcI C'TryA_G
Cuts at: 826 1045 2959 3638 4024 5159 5840 6031 826
Size: 219 1914 679 386 1135 681 191 1471
Fragments arranged by size:
1914 1471 1135 681 679 386 219 191
SfoI GGC'GCC
Cuts at: 3098 3185 3098
Size: 87 6589
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6676
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6676
SmaI CCC'GGG
Cuts at: 1872 4003 1872
Size: 2131 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2428 4780 5681 5943 820
Size: 197 926 485 2352 901 262 1553
Fragments arranged by size:
2352 1553 926 901 485 262 197
SnaBI TAC'GTA
Cuts at: 493 4467 493
Size: 3974 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6676
SphI G_CATG'C
Cuts at: 1132 2737 3670 4034 1132
Size: 1605 933 364 3774
Fragments arranged by size:
3774 1605 933 364
SspI AAT'ATT
Cuts at: 5 52 4600 5
Size: 47 4548 2081
Fragments arranged by size:
4548 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6676
StyI C'CwwG_G
Cuts at: 513 1065 2004 2787 4115 513
Size: 552 939 783 1328 3074
Fragments arranged by size:
3074 1328 939 783 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3854 4703 4720 4873 5058 5503 325
Size: 1255 2274 849 17 153 185 445 1498
Fragments arranged by size:
2274 1498 1255 849 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3375 3429 3501
Size: 274 80 33 51 1443 1398 54 72
Cuts at: 3501 4922 6634 96
Size: 1421 1712 138
Fragments arranged by size:
1712 1443 1421 1398 274 138 80 72
54 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6636 6668 6636
Size: 32 6644
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6676
XbaI T'CTAG_A
Cuts at: 4012 4012
Size: 6676
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6676
XmaI C'CCGG_G
Cuts at: 1870 4001 1870
Size: 2131 4545
XmnI GAAnn'nnTTC
Cuts at: 4805 4805
Size: 6676
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2069 2230 4484 276
Size: 53 83 186 936 535 161 2254 2468
Fragments arranged by size:
2468 2254 936 535 186 161 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BplI BsiWI BsmBI BstZ17I EcoRV FseI FspAI NruI
NsiI PacI PspOMI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SrfI SwaI XcmI