Restriction map for
pFN21AB8304
(Circular) (Six-base) MAPSORT of: pFN21AB8304.seq Check: 8003 from: 1 to: 5599
pFN21AB8304 5599 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3409 278
Size: 53 83 186 936 1873 2468
Fragments arranged by size:
2468 1873 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2942 1937
Size: 1005 4594
Acc65I G'GTAC_C
Cuts at: 2505 2920 2505
Size: 415 5184
AclI AA'CG_TT
Cuts at: 1563 3726 4099 1563
Size: 2163 373 3063
Fragments arranged by size:
3063 2163 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3662 5046 5314 1632
Size: 2030 1384 268 1917
Fragments arranged by size:
2030 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2099 2168 820
Size: 197 1082 69 4251
Fragments arranged by size:
4251 1082 197 69
AflIII A'CryG_T
Cuts at: 1203 4498 5475 1203
Size: 3295 977 1327
Fragments arranged by size:
3295 1327 977
AgeI A'CCGG_T
Cuts at: 4430 4430
Size: 5599
AhdI GACnn_n'nnGTC
Cuts at: 4328 4328
Size: 5599
AleI CACnn'nnGTG
Cuts at: 1166 2552 1166
Size: 1386 4213
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5599
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2454 2486 2454
Size: 32 5567
AlwNI CAG_nnn'CTG
Cuts at: 2401 3342 4914 2401
Size: 941 1572 3086
Fragments arranged by size:
3086 1572 941
ApaI G_GGCC'C
Cuts at: 2608 2737 2608
Size: 129 5470
ApaLI G'TGCA_C
Cuts at: 3656 4812 3656
Size: 1156 4443
ApoI r'AATT_y
Cuts at: 1493 2246 2270 2908 3134 3286 5395 1493
Size: 753 24 638 226 152 2109 1697
Fragments arranged by size:
2109 1697 753 638 226 152 24
AseI AT'TA_AT
Cuts at: 160 1032 4153 160
Size: 872 3121 1606
Fragments arranged by size:
3121 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5599
AvaI C'yCGr_G
Cuts at: 1870 1943 2924 1870
Size: 73 981 4545
Fragments arranged by size:
4545 981 73
BamHI G'GATC_C
Cuts at: 2929 3296 2929
Size: 367 5232
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2505 2920 4375 618
Size: 325 217 627 718 415 1455 1842
Fragments arranged by size:
1842 1455 718 627 415 325 217
BanII G_rGCy'C
Cuts at: 1391 2515 2608 2737 2918 1391
Size: 1124 93 129 181 4072
Fragments arranged by size:
4072 1124 181 129 93
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4628
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3790 3824 1903
Size: 34 1853 34 3678
Fragments arranged by size:
3678 1853 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3491 4701 3491
Size: 1210 4389
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5599
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4210 136
Size: 107 122 71 631 605 2538 1525
Fragments arranged by size:
2538 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2157 2260 5594 2157
Size: 103 3334 2162
Fragments arranged by size:
3334 2162 103
BlpI GC'TnA_GC
Cuts at: 3016 3016
Size: 5599
Bme1580I G_kGCm'C
Cuts at: 1790 2045 2139 2608 2737 3660 4816 1790
Size: 255 94 469 129 923 1156 2573
Fragments arranged by size:
2573 1156 923 469 255 129 94
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5599
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2133 3330 4288
Size: 416 53 83 399 24 716 1197 958
Cuts at: 4288 442
Size: 1753
Fragments arranged by size:
1753 1197 958 716 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2435 1056
Size: 1379 4220
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2324 2584 2637 4259 1239
Size: 1085 260 53 1622 2579
Fragments arranged by size:
2579 1622 1085 260 53
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2249 3724 4589 4887 2249
Size: 1475 865 298 2961
Fragments arranged by size:
2961 1475 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2376 4262 882
Size: 617 877 1886 2219
Fragments arranged by size:
2219 1886 877 617
BsaAI yAC'GTr
Cuts at: 493 1204 2046 3390 493
Size: 711 842 1344 2702
Fragments arranged by size:
2702 1344 842 711
BsaBI GATnn'nnATC
Cuts at: 5593 5593
Size: 5599
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3406 3788 4423 275
Size: 53 83 186 936 1873 382 635 1451
Fragments arranged by size:
1873 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2643 4032 4430 4704 4851 5262 2643
Size: 1389 398 274 147 411 2980
Fragments arranged by size:
2980 1389 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2076 2106 2454 2484 1178
Size: 30 165 30 673 30 348 30 4293
Fragments arranged by size:
4293 673 348 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5599
BseYI C'CCAG_C
Cuts at: 4802 5298 4802
Size: 496 5103
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2237 2789 1163
Size: 573 501 552 3973
Fragments arranged by size:
3973 573 552 501
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3049 3810 4838 1540
Size: 275 186 1048 761 1028 2301
Fragments arranged by size:
2301 1048 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2515 3660 3745 4816 2515
Size: 1145 85 1071 3298
Fragments arranged by size:
3298 1145 1071 85
BsiWI C'GTAC_G
Cuts at: 2046 2046
Size: 5599
BsmI GAATG_Cn'
Cuts at: 2298 3110 3203 2298
Size: 812 93 4694
Fragments arranged by size:
4694 812 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2045 2139 2515 2608 2737 2918 3660
Size: 399 255 94 376 93 129 181 742
Cuts at: 3660 3745 4816 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 376 255 181 129
94 93 85
BspEI T'CCGG_A
Cuts at: 2643 5262 2643
Size: 2619 2980
BspHI T'CATG_A
Cuts at: 3488 5218 3488
Size: 1730 3869
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2954 844
Size: 2110 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3486 1394
Size: 429 1663 3507
Fragments arranged by size:
3507 1663 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4094 4268 65
Size: 1183 2846 174 1396
Fragments arranged by size:
2846 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4243 4430 1700
Size: 110 2433 187 2869
Fragments arranged by size:
2869 2433 187 110
BsrGI T'GTAC_A
Cuts at: 96 2058 96
Size: 1962 3637
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5599
BssSI C'ACGA_G
Cuts at: 3659 4671 3659
Size: 1012 4587
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2803 794
Size: 2009 3590
BstBI TT'CG_AA
Cuts at: 4403 4403
Size: 5599
BstEII G'GTnAC_C
Cuts at: 1184 2494 1184
Size: 1310 4289
BstXI CCAn_nnnn'nTGG
Cuts at: 2554 2554
Size: 5599
BstYI r'GATC_y
Cuts at: 1972 2157 2260 2646 2929 3296 3681 3698 5150
Size: 185 103 386 283 367 385 17 1452
Cuts at: 5150 5236 5248 5594 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 386 385 367 346 283 185
103 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4108
Fragments arranged by size:
4108 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4860
Fragments arranged by size:
4860 683 56
BtsI GCAGTG_nn'
Cuts at: 3115 3909 3929 3115
Size: 794 20 4785
Fragments arranged by size:
4785 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5599
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5564
DraI TTT'AAA
Cuts at: 2903 2903
Size: 5599
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4606 809
Size: 507 3290 1802
Fragments arranged by size:
3290 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2684 2832 3046
Size: 54 1425 50 275 18 854 148 214
Cuts at: 3046 3935 5564 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 854 275 214 148 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3046 1537
Size: 275 1234 4090
Fragments arranged by size:
4090 1234 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2310 2645 3529 1353
Size: 957 335 884 3423
Fragments arranged by size:
3423 957 884 335
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2099 4186 4560 4706 387
Size: 1064 138 510 2087 374 146 1280
Fragments arranged by size:
2087 1280 1064 510 374 146 138
EcoICRI GAG'CTC
Cuts at: 2513 2513
Size: 5599
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2324 2584 2637 3662 4259 5046 5314
Size: 393 692 260 53 1025 597 787 268
Cuts at: 5314 1239
Size: 1524
Fragments arranged by size:
1524 1025 787 692 597 393 268 260
53
EcoO109I rG'GnC_Cy
Cuts at: 2344 2371 2605 2664 2344
Size: 27 234 59 5279
Fragments arranged by size:
5279 234 59 27
EcoRI G'AATT_C
Cuts at: 2908 3286 2908
Size: 378 5221
FspI TGC'GCA
Cuts at: 4105 4105
Size: 5599
HaeII r_GCGC'y
Cuts at: 1678 2099 4746 1678
Size: 421 2647 2531
Fragments arranged by size:
2647 2531 421
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2581 2613 4240 4272 4314
Size: 32 135 32 1009 32 1627 32 42
Cuts at: 4314 4346 1373
Size: 32 2626
Fragments arranged by size:
2626 1627 1009 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2079 2943 3189 669
Size: 988 281 141 864 246 3079
Fragments arranged by size:
3079 988 864 281 246 141
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5599
HpaI GTT'AAC
Cuts at: 3189 3189
Size: 5599
KpnI G_GTAC'C
Cuts at: 2509 2924 2509
Size: 415 5184
MfeI C'AATT_G
Cuts at: 3198 3198
Size: 5599
MluI A'CGCG_T
Cuts at: 5475 5475
Size: 5599
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4713 4897 1305
Size: 3408 184 2007
Fragments arranged by size:
3408 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2686 10
Size: 54 1425 343 854 2923
Fragments arranged by size:
2923 1425 854 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2552 3557 3916 4075 518
Size: 648 1386 1005 359 159 2042
Fragments arranged by size:
2042 1386 1005 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2335 2552 3015 3692 4840 5085 1606
Size: 729 217 463 677 1148 245 2120
Fragments arranged by size:
2120 1148 729 677 463 245 217
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4108
Fragments arranged by size:
4108 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5599
NheI G'CTAG_C
Cuts at: 1052 2431 1052
Size: 1379 4220
NotI GC'GGCC_GC
Cuts at: 3046 3046
Size: 5599
NspI r_CATG'y
Cuts at: 1132 1229 2957 4502 1132
Size: 97 1728 1545 2229
Fragments arranged by size:
2229 1728 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5599
PciI A'CATG_T
Cuts at: 4498 4498
Size: 5599
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2023 2491 1108
Size: 915 468 4216
Fragments arranged by size:
4216 915 468
PfoI T'CCnGG_A
Cuts at: 2862 2862
Size: 5599
PmeI GTTT'AAAC
Cuts at: 2903 2903
Size: 5599
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2454 2486 3668 3700 5181 5213 2454
Size: 32 1182 32 1481 32 2840
Fragments arranged by size:
2840 1481 1182 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2371 2664 2371
Size: 293 5306
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5599
PsiI TTA'TAA
Cuts at: 3169 3169
Size: 5599
PspOMI G'GGCC_C
Cuts at: 2604 2733 2604
Size: 129 5470
PstI C_TGCA'G
Cuts at: 830 2951 830
Size: 2121 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5599
PvuII CAG'CTG
Cuts at: 2335 2552 2335
Size: 217 5382
SacI G_AGCT'C
Cuts at: 2515 2515
Size: 5599
SalI G'TCGA_C
Cuts at: 1936 2941 1936
Size: 1005 4594
SanDI GG'GwC_CC
Cuts at: 2664 2664
Size: 5599
SapI GCTCTTCn'nnn_
Cuts at: 2645 2645
Size: 5599
SbfI CC_TGCA'GG
Cuts at: 2951 2951
Size: 5599
ScaI AGT'ACT
Cuts at: 2143 3847 5559 2143
Size: 1704 1712 2183
Fragments arranged by size:
2183 1712 1704
SexAI A'CCwGG_T
Cuts at: 2235 2235
Size: 5599
SfcI C'TryA_G
Cuts at: 826 1045 2947 4082 4763 4954 826
Size: 219 1902 1135 681 191 1471
Fragments arranged by size:
1902 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5599
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5599
SmaI CCC'GGG
Cuts at: 1872 2926 1872
Size: 1054 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2099 2168 2264 3703 4604 4866
Size: 197 926 156 69 96 1439 901 262
Cuts at: 4866 820
Size: 1553
Fragments arranged by size:
1553 1439 926 901 262 197 156 96
69
SnaBI TAC'GTA
Cuts at: 493 3390 493
Size: 2897 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5599
SphI G_CATG'C
Cuts at: 1132 2957 1132
Size: 1825 3774
SspI AAT'ATT
Cuts at: 5 52 2191 2540 3523 5
Size: 47 2139 349 983 2081
Fragments arranged by size:
2139 2081 983 349 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5599
StyI C'CwwG_G
Cuts at: 513 1065 2004 2835 3038 513
Size: 552 939 831 203 3074
Fragments arranged by size:
3074 939 831 552 203
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2498 3626 3643 3796 3981 4426 325
Size: 1255 918 1128 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1128 918 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2058 2141 3845
Size: 274 80 33 51 1443 81 83 1704
Cuts at: 3845 5557 96
Size: 1712 138
Fragments arranged by size:
1712 1704 1443 274 138 83 81 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2076 2108 2284 2316 5559 5591 2076
Size: 32 176 32 3243 32 2084
Fragments arranged by size:
3243 2084 176 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5599
XbaI T'CTAG_A
Cuts at: 2935 2935
Size: 5599
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2842 2842
Size: 5599
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5599
XmaI C'CCGG_G
Cuts at: 1870 2924 1870
Size: 1054 4545
XmnI GAAnn'nnTTC
Cuts at: 3728 3728
Size: 5599
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3407 276
Size: 53 83 186 936 1873 2468
Fragments arranged by size:
2468 1873 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspXI PsrI RsrII SacII SfiI
SfoI SrfI SwaI